LOCUS       CP115340                2372 bp    DNA     circular BCT 30-AUG-2023
DEFINITION  Escherichia coli strain CUVET16-242 plasmid pCUVET16-242.7, complete
            sequence.
ACCESSION   CP115340
VERSION     CP115340.1
DBLINK      BioProject: PRJNA914526
            BioSample: SAMN32339849
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 17-NOV-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2023 15:24:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,228
            CDSs (total)                      :: 5,103
            Genes (coding)                    :: 4,456
            CDSs (with protein)               :: 4,456
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 647
            CDSs (without protein)            :: 647
            Pseudo Genes (ambiguous residues) :: 0 of 647
            Pseudo Genes (frameshifted)       :: 467 of 647
            Pseudo Genes (incomplete)         :: 191 of 647
            Pseudo Genes (internal stop)      :: 57 of 647
            Pseudo Genes (multiple problems)  :: 60 of 647
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET16-242"
                     /isolation_source="Urinary bladder mucosa"
                     /host="dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET16-242.7"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2016-07-03"
                     /collected_by="Pattrarat Chanchaithong"
     gene            67..1035
                     /locus_tag="PCS78_26125"
     CDS             67..1035
                     /locus_tag="PCS78_26125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WMO56087.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="plasmid recombination protein"
     gene            complement(1105..1335)
                     /locus_tag="PCS78_26130"
     CDS             complement(1105..1335)
                     /locus_tag="PCS78_26130"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WMO56088.1"
                     /translation="MMNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRT
                     SGNKYSTKGLSPKQKNAAFSILKKHGYHLND"
                     /name="hypothetical protein"
     gene            complement(1322..1762)
                     /locus_tag="PCS78_26135"
     CDS             complement(1322..1762)
                     /locus_tag="PCS78_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WMO56086.1"
                     /translation="MDENGEVKQDSKIETFSIPAEPPYVKLYIADIAMIHGLSKSQNDV
                     LLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVDKQIFFRAGRGELEA
                     NPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVMDDEC"
                     /name="hypothetical protein"
     Typing          59..1032
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.077"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 gcccgccgga ggtatcgcaa tgcgctcccc cgcctggctg aatgaatgcc ggaatcaggg
       61 gggaacatgg cgaactacgc gataatgcgc tgcaaaaagc tgacgggaat gggaagcgtg
      121 gccagcgcgt tgcagcactg ctaccgagag cgtgagacgc cgaacgctaa cgctgaacgg
      181 acaccagaaa actactgctc tgtgtccaaa tcaacggatc aggcaatggg gcgtgtgcgg
      241 gagctgctgc cggaaaaacg ccgtaaagat gcggtactgg ccgttgaata cgttatgaca
      301 gcaagccctg agtggtggaa agaggccaca ccacggcagc aggcggagtt tttcgcccgc
      361 tctgagcaat ggctggagaa gaaatacggg aaggatcgtg ttgtggcggc ggtggttcac
      421 cgggacgagg ccaccccgca cctgtctgcg ttcgtggtgc cattgacgca ggatggacgg
      481 ctgtccgcga aagagtttat tggcgggcgg tcaaagatgc gtgaagatca gagcacctac
      541 gctgaatcag taaaaaaact gggtcttgag cgcggcatcg agggaagccg cgccacgcat
      601 cagacagttc agcactacta cgaatcgata aaccgtggca cccgtagcca ggtatcgatc
      661 tcgccggagg cgctggagcc gcgtgtattg cggaagggca ttttcaccaa agacgtggaa
      721 gatcaggcag ccatagccaa acgtctaagc cacgcagtga acgatggctt tgcaggaacc
      781 atcgctatgg cctctcagag cgcccagaac gcaaaaagag cgcgtgagct acagaagact
      841 atggatgcgc agcagaaacg cctacagagc gtcacagagc cttttaaggg gctttccaga
      901 gagcagatga ccgaaatcct gatgatggct cagcggttta agcagcaaaa ccaggaaaag
      961 gaaaaacagc agcgtattga acgtgaaaaa cagcgacaga cgcgatcgcg tggcatgggt
     1021 ggtatggagc gttagttttg atgcgatgca ggatagggct aacgccgcaa agtgacgtca
     1081 tttgcggcat tcaccacgaa aaaattaatc attcagatgg taaccatgtt tttttaaaat
     1141 agaaaaagct gcgtttttct gctttggtga caaaccctta gttgagtatt tattaccgct
     1201 agttctacga tatatgctgt aaacactgct taaaaaagta cgctcccaaa cagtcagaac
     1261 ctcattcttt tttagtgaat catgcagcat tagcctgtct acatttttaa aaagtttatc
     1321 ttcagcattc atcatccatt accttcgtct gcacggttct tccattggtg tcataactga
     1381 tctttaactc aattttcttt cttctttcgt aaattgatgg ccagtctccc cgtgcaaaaa
     1441 gaaaggggtt agcttcaagt tccccacgcc ctgcccggaa gaatatttgt ttatctacaa
     1501 gagccgcaat tatgttttta aaggtctgat attcaatatg aaccctgggc tgtatggttt
     1561 tttcaaatct agtttttgat acagacacaa acccatccca tccaatcagc attgccaatt
     1621 gtaaaagcac atcattttga gattttgata aaccatgaat catcgcaatg tctgcaatgt
     1681 atagcttgac gtagggaggt tcggctggga tggaaaaggt ctcgatctta gagtcttgtt
     1741 ttacctcacc gttttcatcc atgtatgtat gccgttcaag cctagaaatc tttttcgtat
     1801 tcactccagc cccacgtttc gccatgattt agtactcgca tttaagtact aaagagacat
     1861 acagtcaagt cctttgtatc aagtacgatt agtacttgag gtgagtacga ttagtccttg
     1921 aggtgagtac gattagtact tgaggtgagt acgattggta cttgaggtga gtacgatctt
     1981 cgctctagcc cttggtacaa gcgggttccc atatctatat ctatatatta agggggaaaa
     2041 taagggctga aaaacggcat ttttagacac tgaaaaactg ccttttttta gcaaggagtg
     2101 tacgcatttc tatgtacggg gagtggaaat tccgcttcct cgctttggct gttcgttcct
     2161 cgtcgcgttg ctcctgcggt acgcctcagc caaagctgcg gcgagcggtt cctgctcttt
     2221 caaagacgat ttcattgcct gatttcgtca agggcgagcg tagcgagtgc catttaccct
     2281 tgacgacact ccggataaaa caggcttggc agtggtgggg ggtggcgcga agcgacgcct
     2341 tccacctgcc agaccgccgt tgattttgac ct
//
