LOCUS       CP115339                3231 bp    DNA     circular BCT 30-AUG-2023
DEFINITION  Escherichia coli strain CUVET16-242 plasmid pCUVET16-242.6, complete
            sequence.
ACCESSION   CP115339
VERSION     CP115339.1
DBLINK      BioProject: PRJNA914526
            BioSample: SAMN32339849
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 17-NOV-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2023 15:24:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,228
            CDSs (total)                      :: 5,103
            Genes (coding)                    :: 4,456
            CDSs (with protein)               :: 4,456
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 647
            CDSs (without protein)            :: 647
            Pseudo Genes (ambiguous residues) :: 0 of 647
            Pseudo Genes (frameshifted)       :: 467 of 647
            Pseudo Genes (incomplete)         :: 191 of 647
            Pseudo Genes (internal stop)      :: 57 of 647
            Pseudo Genes (multiple problems)  :: 60 of 647
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET16-242"
                     /isolation_source="Urinary bladder mucosa"
                     /host="dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET16-242.6"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2016-07-03"
                     /collected_by="Pattrarat Chanchaithong"
     gene            join(2915..3231,1..4)
                     /gene="mobA"
                     /locus_tag="PCS78_26100"
                     /pseudo=""
     CDS             join(2915..3231,1..4)
                     /gene="mobA"
                     /locus_tag="PCS78_26100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology. GO_process:
                     GO:0006355 - regulation of DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /name="mobA"
     gene            12..194
                     /locus_tag="PCS78_26105"
     CDS             12..194
                     /locus_tag="PCS78_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WMO56083.1"
                     /translation="MNAVSPQTDRHSVSCIVMQRAENNPDAFFRFTPGKTSLFAVFAAE
                     SRSARFCGARNATLW"
                     /name="hypothetical protein"
     gene            complement(186..764)
                     /locus_tag="PCS78_26110"
     CDS             complement(186..764)
                     /locus_tag="PCS78_26110"
                     /inference="COORDINATES: protein motif:HMM:NF025107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WMO56084.1"
                     /translation="MKSEIELKKTEHSSVEIPERILAIWGRSILNSGWTSVPNELLKNQ
                     SKLGIGNTELVLLINLISFMHHPDARVYPSISLLCERMNQDRRTIQRNLNKLVEMDILR
                     IKIRSTGKNSKGMTNLYDITPLMLKLINLKIPSLNTPDEKHTCPKCGKIAISREEITKE
                     FGFRTDTNGKMRTQSWCKDCRGRKIADLP"
                     /name="helix-turn-helix domain-containing protein"
     gene            1162..1614
                     /locus_tag="PCS78_26115"
     CDS             1162..1614
                     /locus_tag="PCS78_26115"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WMO56085.1"
                     /translation="MTTKFNKFELHSAFRDGKLNEKYFVDLPYLKEEQRRALASFLGAI
                     TREEPLPGKNKPSWLDDNSIKISGTDAYEDGNYWHYHCGPYPEGVKLRSMTFNLGMNVA
                     GLTSEVIHYQKLNDGTVFVVGFSPTHEPFPKSDAPHYNPLFTESDD"
                     /name="hypothetical protein"
     gene            complement(2448..2642)
                     /locus_tag="PCS78_26120"
     CDS             complement(2448..2642)
                     /locus_tag="PCS78_26120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WMO56082.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEVENSTEI"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2680..2906
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="88.797"
                     /coverage="99.0"
                     /evalue="6.34e-74"
     Typing          complement(1891..2419)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.701"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          2916..3229
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.048"
                     /coverage="98.0"
                     /evalue="2.4e-61"
ORIGIN
        1 aaacagacga tatgaacgcc gtaagccccc aaaccgatcg ccattcagtt tcatgcatag
       61 tcatgcaacg cgctgaaaac aatcctgacg catttttccg gtttaccccg gggaaaacat
      121 ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg tttctgcggt gcgcgtaatg
      181 cgacgttatg gtaaatccgc aattttccta cctcgacaat ccttgcacca actttgcgtt
      241 ctcatcttcc cattagtgtc agttctgaat ccaaactcct ttgttatttc ttctcttgat
      301 atggctattt ttccgcattt tggacacgta tgtttttcat cgggcgtatt taaagatggt
      361 atttttaggt tgattaactt cagcattaaa ggggttatgt catacaagtt agtcattcct
      421 ttagagtttt tgccggtgga gcgtattttt atcctcagaa tgtccatttc tactaacttg
      481 tttaaatttc tctgaattgt tcgtctgtcc tgattcattc tttcgcatag aagtgaaatt
      541 gatgggtaca ctcttgcgtc aggatgatgc atgaaagata tcagattaat caaaagtaca
      601 agctctgtgt ttcctattcc tagcttagat tggtttttta atagttcatt ggggacggat
      661 gtccagccag agtttaaaat gctgcgcccc catatggcta agatcctttc tgggatctct
      721 actgatgagt gttctgtttt tttgagttcg atttctgatt tcaatttctg tcaccaatca
      781 taataacaat acctatatat ataggtattg ttattatgat ttattatttg ggcggcatca
      841 atgccgccct ttaatttgac caaaaaggcc taatgtctag cactgggaaa ggacctctct
      901 taccatctaa ttgagtaaga ttttatcttc ccagacttct tcaaacatag gactgacatg
      961 ttggcaaacg tacgatttga taatcacagc tgcgatacag aacgtcgtca gcagtacaaa
     1021 tctcgtgctg acgaagtgct gctctcgcgt tcatacaaga atcaggcaca tgaagcgtta
     1081 aaatgcgatt ctccgcagtc tgatgaatcg aatctttgat atgctatcta cttactcagt
     1141 aaggtaagtg gaaagtgttt gatgacaacc aaattcaata agtttgagct tcattcggct
     1201 ttccgagacg ggaagctgaa tgaaaagtat ttcgtagatt taccctacct caaagaagag
     1261 caacgccggg cattagcctc tttcttgggt gcgataactc gtgaagagcc tcttccaggg
     1321 aaaaacaagc cttcatggct tgatgataat tccattaaaa tttccggtac tgatgcgtac
     1381 gaagacggga actactggca ttatcattgc ggtccatatc cagaaggggt caaacttaga
     1441 tcgatgactt ttaacttggg tatgaacgtt gcggggctaa cctctgaagt gatccactat
     1501 caaaaactca acgatggcac ggtgtttgtc gtaggcttct cccctacaca tgagcccttc
     1561 cctaagtctg atgcgccgca ttacaaccca ctcttcactg aatcagatga ttaaatatct
     1621 gaatagtggc tggtaaggat atgttgagat ttttttctct cagctgtagt ctcttttcct
     1681 gaaacgaaaa aaccacctgg ggaggtggtt tttcgaaggt tcagtaagtt ggggaacttc
     1741 tgaaccgtgg taacagtctt gtgcgagaca tgtcaccaaa tctgtccttt cagtgtagcc
     1801 gcagtcaggc catcacttca agaactctcg atacatctct cgcacatcct gtctaccaat
     1861 ggccgcagcc aatggcgata agtcgtgtct ttccgggttg gactcaagac gatagttacc
     1921 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1981 aacgacctac accgaactga gataccaaca gcgtgagcta tgagaaagcg ccacgcttcc
     2041 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2101 gagggagctt ccaggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2161 tgacttgagc gtcggttttt gtgatgctcg tcagggggcg gagcctatgg aaaaacgcca
     2221 tcggcgcggc ctttgtccct ggcttcggct ttttgctcac atgttctttc cggcgttatc
     2281 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgacaccg ctcgccgcag
     2341 tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaagagcgcc ttatgtgaca
     2401 ttttctcctt acgctctgtt gtgccgttcg gcatcctgcc ctgagcgtta tatctctgtg
     2461 ctattttcta cttcaaagcg tgtctgtatg ctgttctgga gttcttctgc gagttcgtgc
     2521 agcttctcac acatggcggc ctgctcgtca gcatcgagtg cgtccagttt ttcgagcagc
     2581 gtcaggctct ggctttttat gaatcccgcc atgttgagta cagtttgctg ctgcttgttc
     2641 atctttctgt tttctccgtt ctgtctgtca tctgcgtcgt gtgattatat cgcgcaccac
     2701 ttttggaccg tcttaccgcc ggtattctgc cgacggacat ttcagtcaga caacactgtc
     2761 actgccaaaa aaacagcagt gctttgttgg taattcgaac ttgcagacag gacaggatgt
     2821 gcaattgtta taccgcgcat acatgcacgc tattacaatt accctggtca gggcttcgcc
     2881 ccgacacccc atgtcagata cggagccatg ttttatgaca aaacgaagtg gaagtaatac
     2941 gcgcaggcgg gctatcagtc gccctgttcg tctgacggca gaagaagacc aggaaatcag
     3001 aaaaagggct gctgaatgcg gcaagaccgt ttctggtttt ttacgggcgg cagctctcgg
     3061 taagaaagtt aactcactga ctgatgaccg ggtactgaaa gaagttatga gactgggggc
     3121 gttacagaaa aaactcttta tcgacggcaa gcgtgtcggg gacagggagt atgcggaggt
     3181 gctgatcgct attacggagt atcaccgtgc cctgttatcc aggcttatgg c
//
