LOCUS       CP115338                4021 bp    DNA     circular BCT 30-AUG-2023
DEFINITION  Escherichia coli strain CUVET16-242 plasmid pCUVET16-242.5, complete
            sequence.
ACCESSION   CP115338
VERSION     CP115338.1
DBLINK      BioProject: PRJNA914526
            BioSample: SAMN32339849
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 17-NOV-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2023 15:24:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,228
            CDSs (total)                      :: 5,103
            Genes (coding)                    :: 4,456
            CDSs (with protein)               :: 4,456
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 647
            CDSs (without protein)            :: 647
            Pseudo Genes (ambiguous residues) :: 0 of 647
            Pseudo Genes (frameshifted)       :: 467 of 647
            Pseudo Genes (incomplete)         :: 191 of 647
            Pseudo Genes (internal stop)      :: 57 of 647
            Pseudo Genes (multiple problems)  :: 60 of 647
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET16-242"
                     /isolation_source="Urinary bladder mucosa"
                     /host="dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET16-242.5"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2016-07-03"
                     /collected_by="Pattrarat Chanchaithong"
     gene            complement(419..622)
                     /locus_tag="PCS78_26075"
     CDS             complement(419..622)
                     /locus_tag="PCS78_26075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron Se72 family effector protein"
                     /protein_id="WMO56080.1"
                     /translation="MENGFVNFYDHVKGYGFIRRERGRDVFFRYDDFLFLGHDVDICKG
                     ILVRFKLEKTDKGFKAVAIQKV"
                     /name="retron Se72 family effector protein"
     gene            complement(627..1580)
                     /locus_tag="PCS78_26080"
     CDS             complement(627..1580)
                     /locus_tag="PCS78_26080"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="WMO56081.1"
                     /translation="MKGIQKKINKEIFEKIDYPHYLHGALSGRDYISNAAVHTRKRTVI
                     CLDITNFYPSISKKDVCSIFKNLMRFSPDVSLYLTELVTLNNKVPQGGCCSSYIANLLF
                     FNSEYNLYNRLKSMGLSYSRLLDDITISSDKDFSSEEKTKVIKLVHGMVNQYRLSINES
                     KTTIEHSKDSSSKLSVTGLWVKHGVPKLTKERRYIRYLVYICKKQGAYERHTKEYHDLW
                     NRCSGKVAQMSRLGHVQAVELRAILSEIMPVYDDYKISKLKLMAKHYLNKFTPPLTDDQ
                     IRKIDRMLYDFDIVGRTNKNLAKLYRRKLVALLPDR"
                     /name="reverse transcriptase family protein"
     gene            complement(2335..2601)
                     /locus_tag="PCS78_26085"
     CDS             complement(2335..2601)
                     /locus_tag="PCS78_26085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WMO56077.1"
                     /translation="MVKLLLVRNEYFNKNNDLKTVARFDGKTLRDAFSRKIATFDGKVL
                     RDASLKKVATFDGQNLRDASSKIILSADAEVGIVVMAFVAELI"
                     /name="hypothetical protein"
     gene            complement(2826..3149)
                     /gene="mobA"
                     /locus_tag="PCS78_26090"
     CDS             complement(2826..3149)
                     /gene="mobA"
                     /locus_tag="PCS78_26090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006355 - regulation of DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WMO56078.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            3433..3627
                     /locus_tag="PCS78_26095"
     CDS             3433..3627
                     /locus_tag="PCS78_26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WMO56079.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAENRTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(3159..3398)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.755"
                     /coverage="100.0"
                     /evalue="7.66e-118"
     Typing          2..220
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.545"
                     /coverage="86.0"
                     /evalue="3.31e-109"
     Typing          3663..3979
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.375"
                     /coverage="90.0"
                     /evalue="3.06e-135"
     Typing          complement(2830..3149)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="3.07e-62"
ORIGIN
        1 gcgctttctc atagctcacg ctgttggtat ctcggcccgg tgtaggtcgt tcgctccaag
       61 ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat
      121 cgacttgaat ccaaaccgag aagacacgac ttatcgccac tggcagcagc cactggtaac
      181 cgaattagag gagttagcct tgaagtcatg cgcctgttaa ggctaaactg aaagaacaag
      241 ttttggtgac tgcgctcctc caagccagtt acctcggctc aaagagttgg tagctctgag
      301 aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt acacagcaag agattacgac
      361 gagatagaaa ggatctcaag ttctcatatc ataaaaacaa gctgacacat ccacgatgtt
      421 ataccttttg aatcgctaca gctttaaacc ctttatctgt tttttctaat ttaaacctaa
      481 ctaaaatacc tttacatata tcgacatcat gccctaaaaa caaaaaatca tcataccgaa
      541 agaaaacatc acgtccacgc tcacgcctta taaagccata ccctttaaca tggtcataaa
      601 aattaacaaa tccattttcc atataatcac ctatctggaa gtaatgccac gagcttacgt
      661 ctatatagtt ttgcaagatt cttattagtc cgccctacta tatcaaaatc atagagcatc
      721 ctatctattt tcctaatctg atcatcagtt aaaggcggcg taaacttatt tagatagtgc
      781 ttagccatca actttaattt agatatcttg taatcatcat aaactggcat tatctcagaa
      841 agaatcgccc tcaattcaac cgcctggaca tgcccaagcc tcgacatttg agcaaccttg
      901 ccagagcatc tattccataa atcatggtac tctttcgtat gacgttcata agcaccttgt
      961 tttttacata tataaactaa gtacctgata taacgtcttt cttttgttaa cttaggaaca
     1021 ccatgcttta cccacaaccc agtgacacta agtttagaag acgaatcttt tgagtgctct
     1081 atcgttgtct tactttcatt tattgaaagt ctgtactgat tcaccattcc gtgaacgagt
     1141 tttattactt ttgttttttc ctctgatgaa aaatccttat cagaagatat agtaatatca
     1201 tctaaaagcc ttgaatatga aagccccata gacttcaatc tattatagag attatactca
     1261 ctattaaaaa ataaaagatt tgcaatataa ctagaacagc acccaccctg agggacctta
     1321 ttattcaacg tcacaagttc cgttaagtat aaagaaacat caggagaaaa acgcattaaa
     1381 tttttaaaaa tagaacaaac atctttttta ctaatgctgg gataaaaatt ggtgatatca
     1441 agacatataa ctgttctttt cctagtatga acagcggcat tagatatata atcgcgacca
     1501 cttaacgccc catgcaaata atgagggtaa tcaattttct caaaaatctc tttatttatt
     1561 tttttttgaa ttcctttcaa aaattcttgg gttcaaaaat ttgcctttct tttttattct
     1621 tccctgtttc aaccttaaac tgcttgtacg acatcgatac agacttgact atccagtcca
     1681 atctttttct ttctatgcct aacatcgcag ataaagaatc caaagatgca acaggagttc
     1741 cattaaacct gggtttattc atgatgtttg agttaaaagt cgattagtta aaaagtttcg
     1801 atacgtaatc tattacttta aacataatca agcacataga aaacaaaaac tgtgtttttt
     1861 tcagtgcctc cagagagcca acctctcttt ttttcttcat ctgaccgtct ccagaacaag
     1921 atgaagagct cttggttacg ttaatgttga tgttgataat aaacatgatg caactcctat
     1981 tggttatgaa attttgccag accagtagga aagcaaggaa aaactctatt ggctctttgg
     2041 gacttttaac tcacattgcc agacaatcgt cgctattctt ttagtgctga tacacgctca
     2101 ggggcgttct caccattaat tagcggcagg ccggtcatga tctcatgcga gatcgcctta
     2161 aaaacaaggc atcggtttcc ttgcgcaaca tacacgtaac catttgttaa agagcgagag
     2221 ccaggcgtgg ttcgcccgct tcgctgatta ttataaagtt ttttatcttt ctgtcaccaa
     2281 caagaaagcg ccttatcctg ccagcgatag gctaaaaggt atgcagcatg agattcaaat
     2341 caactcagct acaaaggcca taacaacaat cccaacctca gcatcggcac tcaggataat
     2401 ttttgaacta gcatctctta aattctgacc gtcaaaggtt gctacttttt ttaaagaggc
     2461 atcccttaaa accttgccat caaaagtcgc tatttttctc gaaaaggcat ctcttaaggt
     2521 tttaccgtca aaccgagcaa cggtttttaa gtcattgttt ttattgaaat attcatttct
     2581 caccaatagt aatttaacca attgtattta ccataatccc ttaattgtac gcaccacaga
     2641 atcgcgctca gcgcgattct gacacagaca ctgcaaaaag agatgtttta ccctaagaaa
     2701 accggggaaa tgcgtcagga tcgttttcag ctcgctgcat agctatgcat gaaagcgagt
     2761 ggtgatcact ttgggagctt acagtgttca taccgtctgt tttcgacagt tttctcccct
     2821 ggaagctaat ctgccataag cctggataac agggcacggt gatactccgt aatagcgatc
     2881 agcacctccg catactccct gtccccgaca cgcttgccgt cgataaagag ttttttctgt
     2941 aacgccccca gtctcataac ttctttcagt acccgatcat cagtcagtga gttaactttc
     3001 ttaccgagag ctgccgcccg taaaaaaccg gaaacggtct tgccgcattc agcagccctt
     3061 tttctgattt cctggtcttc ttctgccgtc agacgaacag ggcgactgat agcccgcctg
     3121 cgcgtattac ttccacttcg ttttgtcata aaacatggct ccgtatctga catgggtgtc
     3181 ggggcaaagc cctgaccagg gtaattgtaa tagcgtgcat gtatgcgcgg tataacaatt
     3241 gcacatcctg tcctgtcagc aagctcaaat taccgacaaa ggcacggcag ttttttggca
     3301 gtgacagtgt tgtctgacag aaatgcccgt caaagagtca gacagcagaa taccgacggt
     3361 aaaacagtcg aaaagtggta cgcgatataa tcacacaacg cagatgacag aaagaacgga
     3421 gaaaacagaa agatgaacaa gcagcagcaa accgtactca acatggcagg attcataaaa
     3481 agccagagcc tgacgctgct cgaaaaactg gacgcacttg atgctgacga acaggccgcc
     3541 atgtgtgaga agctgcacga actcgcggaa gaacttcaga acagcataca gacacgcttt
     3601 gaagcggaaa atcgcaccgg gatataacgc atccggacag gatgcagaac ggcatagagg
     3661 agcgtaagga gaaaatgtca cataaggcgc tcttccgctt cctcgctcac tgactcgcta
     3721 cgctcggtcg ttcgactgcg gcgagcggtg tcagctcact caaaggcggt aatacggtta
     3781 tccacagaat caggggataa cgccggaaag aacatgtgag caaaaagccg aagccaggga
     3841 caaaggccgc gccgatggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3901 accgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3961 cccctggaag ctccctcgtt gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4021 a
//
