LOCUS       CP115337                3811 bp    DNA     circular BCT 30-AUG-2023
DEFINITION  Escherichia coli strain CUVET16-242 plasmid pCUVET16-242.4, complete
            sequence.
ACCESSION   CP115337
VERSION     CP115337.1
DBLINK      BioProject: PRJNA914526
            BioSample: SAMN32339849
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 17-NOV-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2023 15:24:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,228
            CDSs (total)                      :: 5,103
            Genes (coding)                    :: 4,456
            CDSs (with protein)               :: 4,456
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 647
            CDSs (without protein)            :: 647
            Pseudo Genes (ambiguous residues) :: 0 of 647
            Pseudo Genes (frameshifted)       :: 467 of 647
            Pseudo Genes (incomplete)         :: 191 of 647
            Pseudo Genes (internal stop)      :: 57 of 647
            Pseudo Genes (multiple problems)  :: 60 of 647
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET16-242"
                     /isolation_source="Urinary bladder mucosa"
                     /host="dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET16-242.4"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2016-07-03"
                     /collected_by="Pattrarat Chanchaithong"
     gene            join(3610..3811,1..89)
                     /locus_tag="PCS78_26060"
     CDS             join(3610..3811,1..89)
                     /locus_tag="PCS78_26060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WMO56075.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVFIGFLFSALQVIHRLAVKFLDLVFNTCF"
                     /name="mobilization protein"
     gene            324..1031
                     /locus_tag="PCS78_26065"
     CDS             324..1031
                     /locus_tag="PCS78_26065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WMO56076.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVASP
                     ELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRSTGE
                     RIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTVVALH
                     FRFKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1286..3431)
                     /locus_tag="PCS78_26070"
                     /pseudo=""
     CDS             complement(1286..3431)
                     /locus_tag="PCS78_26070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     Typing          complement(3074..3432)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.667"
                     /coverage="100.0"
                     /evalue="8.58e-169"
     Typing          3433..3609
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.315"
                     /coverage="100.0"
                     /evalue="5.65e-83"
     Typing          325..1031
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.294"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1814..3000)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.75"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttttattgg gtttttattt tcagcactcc aagttatcca caggctcgcc gttaagtttt
       61 tagatcttgt ttttaatact tgtttttaat acttgtttta gggtcgaaat tcctgattaa
      121 aaacaatgcg ttacacgtgt ataggtgggg aaatttaagg taaaggtgat ataatttaag
      181 gttcttgggt gaggtaattt aaggtaaagg tgaggagatt taaggtaata ttcacagaaa
      241 aatggggggt ggaaatataa ttgctcacca atgctaaagg tgataggttt ttatctcacc
      301 tcatctttgg cctggtggtt aaaatgacat caaaccctct gatagcctat aagtccaatg
      361 cgctggttga ggccagttac aagctaactt tacaagaaca gcgtttctta ttgctctgca
      421 taagccgact taactccggt actgacgtag cttcacctga attgcaaaag acaatgacca
      481 ttacagctgc ggaatatttt gattcattcc ctgatatggg gcggaaaaat gccgaggttc
      541 agctacagga agcgatcgat cgtttgtggg acaggtcgat cattctcaag gacgacgaga
      601 agcgcgagga gttccgctgg attcagtatc gggcacagta cgcgagggga gaggggaaag
      661 cacaaatcac tttttctgac gcggtaatgc cttatctgac acagttgaag gggcaattca
      721 ccagagttgt tattaaaaat atctccaatt tatcaagaag ttacagcata agaatatacg
      781 agattctcca gcaattccgc agtactgggg aacgtattat cgccctcgat gattttaaat
      841 catcactgat gcttgatggt aaatataaag atttcaaaac gttaaacagg gatctaataa
      901 aaccctgtgt tgacgaactg aacaaaaaaa gcgacctggc tgtaacggtt gagacgatca
      961 aaaaggggcg cacggttgta gccctgcatt ttcgcttcaa ggaagacaaa cagatcaaga
     1021 tgacgatcta gaatcgattt taaggcacct aaaacgctct ctgttgcgtt ttttccatga
     1081 gaggtatgcg atggtactac tggtgagata aaattgcttc ataatcgatt ctatggcgtt
     1141 caaaaaaaag aacccccaac ttagtggggg cgggaaaacg ataaagttgg atttgtgcaa
     1201 tctgtgtgca tctgacattc atccagttac gatgaatgtc agagaacata acacgatgta
     1261 aaacaaaaca aaatactatc agcagttacc gtgaacagtc gcgatcattc ttcttcccgg
     1321 agacacgtgt tcgtgcatgc tcctcgcgcc gccattcatc ccaggtgtga ttaaagccca
     1381 gtgaacggaa cagatgatcc ggctgttttt ctggtgtgtt gacggcagca aaaagcggtg
     1441 tctcgcgggt tttaagatga aacgccataa gttccggtgc aggtgaaatg tggttgtctg
     1501 catcgtgatt tacggcaaca ccatatcctg catatcgtaa ccccttatcg gtcagcgcac
     1561 tgatgaccag atgcctgtta tcggcggagg ggacagcaaa cgcgggatac agcgaaccat
     1621 taagccggac aacaccgccc agagagtcat ccacgcccat gaccagcaaa agctgaagtt
     1681 gtcggttttc ccggtcgaca gaactcatta ccggcatcag cgtcggtggc agacgtccct
     1741 gcgtaaaatc caccctcctt gccaccggtg atggcatggc tggctggcgg ttttctccaa
     1801 gacagaaaga cttggccatg tcttcggctg gcggagattg cgggtatgtt gcggcgatgt
     1861 ctggcaaacc tgcgtcagaa gttgctcaaa ggtcgcagag gaataaacac gttgcagtgg
     1921 tggatgttga ccgatttcgc ttcggtcaca gatataacca ctcctgtcac gcattaaatc
     1981 aacactctca aagatcctgg tgcggtattt ctccagctcg ttcccccaga gcgtatattc
     2041 tccgttccgg ttgtgaagca gaaggtaaca ggtcatttcc ccgttaccag gcatgggagc
     2101 ctcgccatat cccgtaacga ctgctctcag cacccctgaa tccataacgg cattgtcatt
     2161 tttcctgagc tgctccgcgc gactacgttc tgtatcctcc agctccttcc ggcgcaattc
     2221 ctcttcacgt ttaagcaact gggatctgag agctgcaagg ccggattccc cccactgctg
     2281 ccactgtttt tcaaatgccg tctgactcgc gggaatgtcc tgtttctcca ccttccggta
     2341 atgctgtcgc aatttttcca tttgctggct cagagactgg atttttccct gctcactacg
     2401 gtattccggt gcagcccatt gcatcagggg aatacgggaa aaaccggacg aaaacggcga
     2461 tgattttttc aggctggcag catggctcat cgcttccttt cgttcggtgt tcagctcttc
     2521 ccagcgtctg cgtgtcgcct gcatatctgc catcatcgac atggcgttac tggtgagttt
     2581 ccatgccgcg ttactgtcct tcggcaacgc ttcgatgatg tcacgtagtg tgtacgaagt
     2641 tgcgttgtca ggaagcccga tcttcagaag gtcagccaca cggcgggcag gctctgtcag
     2701 cccgctgttc ctgagttcgg tgcgggaaat ctgtatttcc aggttaagcc tgttaatcag
     2761 acgattctgt tcgccgcgtt ctgtttcgat accgcgtgtt tccatcgcac aggcagactt
     2821 tccgaggtga atacccggcg ttttctcaag cccctgtcgc tcgtatgaac gatggtctat
     2881 ctcttcctga taaccggcgt tcgccagtgc cctgtttgca tggtcagccc atgatgcacg
     2941 ccacgtctcg gcatgtgtcc ggtcgttcca gtccctgacc tttccgccga acccagcagg
     3001 ccgacagccc tcgtggtcag catgatgtgc gcatggggat ttgttttatc catgtgatgg
     3061 aacgccacat cagcaatcat gccctgactg acaaagtttt ctctgacaaa ggcgagaacg
     3121 gtttcccgtg cggcatcctg cggcagttcg tgggggatgg cgagttcaat ttcccgcgca
     3181 agttgtgagt tcttccggcg ttcggccttc tcaacggcat tccagagttc ggcacgattc
     3241 gtacaccagg caggggctgt cgcgggggtc agaatcacag cctcagccac accgcttttg
     3301 cgggtgtagt cgtgagtcag tccggtacgc tcatcaagca ttagtgaact agaacgatag
     3361 gcagcagaag caacggcgct gtagccgttg cttctggaga tgattttcat actgagatga
     3421 taaattgcca tttctgccgt aatgcctttt tgcagttttc tttcagcccc gcagggcgca
     3481 cacaattgaa acggagtgga aattgtataa gtgcgcactt cgtgatttgc cgtgcattat
     3541 gccagctcag tttttaaact accagtgacg cgctaaaatg cgatttttca ttgcgcatgg
     3601 agttacatca tgagcagaac gctcgaacag aagattgctg atgctgaggc gagattacag
     3661 cgcctgaagg cgaagagccg gagcctggac acagcgcaga aggtcattgt gggtgcagca
     3721 ttgctggcaa aggtcagaaa gccggaggaa gtgcagttgc gagcttggtt actccagttc
     3781 ctgaaggcag aggtgacacg gcaggctgat g
//
