LOCUS       CP114350                4072 bp    DNA     circular BCT 29-DEC-2022
DEFINITION  Escherichia coli strain 2022CK-00559 plasmid unnamed3, complete
            sequence.
ACCESSION   CP114350
VERSION     CP114350.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN31390131
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     2022CK-00559
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2022) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
REFERENCE   3  (bases 1 to 4072)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 47.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/14/2022 17:31:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,415
            CDSs (with protein)               :: 4,415
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 211
            CDSs (without protein)            :: 211
            Pseudo Genes (ambiguous residues) :: 0 of 211
            Pseudo Genes (frameshifted)       :: 74 of 211
            Pseudo Genes (incomplete)         :: 137 of 211
            Pseudo Genes (internal stop)      :: 48 of 211
            Pseudo Genes (multiple problems)  :: 45 of 211
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00559"
                     /isolation_source="Wound/Abscess"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA"
                     /collection_date="2022-04-05"
     gene            116..808
                     /locus_tag="OIX73_23730"
     CDS             116..808
                     /locus_tag="OIX73_23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WBD27771.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1064..3223)
                     /locus_tag="OIX73_23735"
     CDS             complement(1064..3223)
                     /locus_tag="OIX73_23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WBD27772.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            3402..3656
                     /gene="mobC"
                     /locus_tag="OIX73_23740"
     CDS             3402..3656
                     /gene="mobC"
                     /locus_tag="OIX73_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WBD27770.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2866..3224)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          3225..3401
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          117..808
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1068..3310)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.332"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggtggaggaa tttaaggtgt tattcacaga aatgtgagga tataaaaaaa aatattccac
       61 atttcttatt tgtggggcac attaaagcat caccacattt gaacgggatc tttgtatgtc
      121 ggacttgatt gcctacaagt cgaatgcttt ggttgaggcc agctacaagt tgaccctaca
      181 ggaacaacgt tttctgttgc tgtgtatcag tcgattgaaa tctggtgctg atgctgaatt
      241 gcaaaagaca atgaccatta ccgctgcgga atattttgat tcattccctg acatggggcg
      301 gaaaaatgcc gaggttcagt tacaggaagc gatcgatcgt ttatgggaca ggtcgatcat
      361 tctcaagaat gacgagaagc gtgaggagtt ccgctggatc cagtatcggg cacagtacgc
      421 taaaggtgag gccaaggctc gaataacctt ttctgacgcg gtaatgcctt atctgacgca
      481 actgaagggg caattcacgc gcgttgttat aaaaaacata tcaggtttat caagtagtta
      541 cagtataaga atatacgagt tcctccagca attccgcagt acgggagaga gaattattgc
      601 tcttaatgac tttcgttcaa tgttagggat tgaaaataaa tacaaacaat tcagagactt
      661 aaataaaata ctaataaaac cctgtgttga cgaactgaac aaaaaaagcg acctggctgt
      721 aacggttgag acgatcaaaa aggggcgcac ggttgtagcc ctgcattttc gcttcaagga
      781 agacaaacag atcaagatga cgatctaaaa tcgattttaa ggcacctaaa acgctccctg
      841 ctgcgttttt tttgtgttag tagtgcgaag gtactattga agtgaaaaat tgcgtcaaaa
      901 gcgctctaat gcagcgaaaa aagaaccccc aatttttggg ggcggaaaaa caaaactatg
      961 cttgcattat gtgtgtgctt cttgcattca tccctgtcga tgaacataga agaacatcga
     1021 cgaacataac acgatgttaa acaaaacaaa atattgttca cattcactgt gaccaaccgc
     1081 gatcatgtct cctctcggtg gtgtgtgttc ttgcatcttc ctcgcgccgc cattcatccc
     1141 aggtacgatt gaaacccaga gagcggaaca gatgatccgg ctgtttttct ggtgtgttga
     1201 cggcagcaaa aagcggtgcc tcacgggttt tcagatgaaa ctccataagt tccggcgcag
     1261 gtgatatgtg gctgtctgca tcgtgattta cggcaacacc ataccctgca taccgtaacc
     1321 ccttatcggt cagtgcactg atgaccagct ggctgttatc ggctgaggga acggcaaacg
     1381 cgggatacag cgtaccatta agccggacaa caccgcccag agagtcatcc acgcccatga
     1441 ccagcaaaag ctgaagctgg cgggtttccc ggtcgacaga actcattacc ggcatcagcg
     1501 tcggtagcag acgtccctgc gtaaaatcca ccttccttgc tgccagtgat ggcatggctg
     1561 gctggcggtc ttctccaaga cagaaagact ccggccatgt cttcggctgg cggagattgc
     1621 gggtatattg cggccatgtc tggcaaacct gcgtcagaag ttgctcaaag gtcgcagagg
     1681 aataaacacg ttgcagtggt ggatgttgac cgatttcgct tcggtcacag atataaccac
     1741 tcctgtcacg cattaaatca acacgctcaa agattctggt gcggtatttc tccagctcgt
     1801 tcccccagag cgtgtattct ccgttccggt tgtgaagcag aaggtaacag gtcatttccc
     1861 cgttaccagg catgggggcc tcgccatacc ccgtaacgac tgcgctcagt acccctgaat
     1921 ccataacggc attgtcattt ttcctgagct gttccgctcg tcgctccgcg cgactacgtt
     1981 ctgtatcctc ctgctccttc cggcgcaatt cctcttcacg tttaagcaac tggactctga
     2041 gagctgcaag gccggatgcc ccccactgct gccactgttt ttcaaatgcc gtctgactcg
     2101 cgggaatgtc ctgtttctcc accttccggt aatgctgtcg caatttttcc atttgctggc
     2161 tcagcgactg gatttttccc tgttcgctac ggtattccgg tgccgcccat tgcatcaggg
     2221 gaatacggga aaaaccggac gaaaacggcg atgatttttt caggctggca gcatggctca
     2281 tcgcttcctt tcgttcggtg ttcagctctt cccagcgtct gcgcgtcgcc tgcatatctg
     2341 ccatcatcga catggcatta ctggtgagtt tccatgccgc attactgtcc ttcggcaacg
     2401 cttcgatgat gtcacgtagt gtgtacgaag ttgcgttgtc aggaagcacg atattcagaa
     2461 ggtcagccac acggcgggca ggctctgtca gcccgctgtt cctgagttca gtgcgggaaa
     2521 tctgtatttc caggttaagc ctgttaatca gacgattctg ttcgccgcgt tctgtttcga
     2581 taccgcgtgt ttccatcgca caggcagact ttccgaggtg aataccaggc gttttctcaa
     2641 gcccctgtcg ctcgtatgaa cgatggtcta tctcttcctg ataaccggcg ttcgccagtg
     2701 ctctgtttgc atggtcagcc catgatgcac gccacgtctc ggcatgtgtc cggtcgttcc
     2761 agtccctgac ctttccgccg aacccagcag gtccgacagc cctcgtggtc agcatgatgt
     2821 gcgcatgggg atttgtttta tccatgtgat ggaacgccac atcagcaatc atgccctgac
     2881 tgacaaagtt ttctctgaca aaggcgagaa cggtttcccg tgcggcatcc tgcggcagtt
     2941 cacgggggat agcgagttca atttcccgcg caagttgtga gttcttccgg cattcggcct
     3001 tctcaacggc attccagagt tcggcacggt tcgtacacca ggcaggggct gtcgcggggg
     3061 tcagaatcac agcttcagcc acaccgcttt tgcgggtgta gtcgtgagtc agtccggtac
     3121 gctcatcaag catcagtgaa ccggaacgat aggcagcgga agctacggcg ctgtagccgc
     3181 tgcttctgga gataattttc atgctgagat gataaatcgc catttctgcc gtaatgcctt
     3241 tttgtagttt tctttcagcc ccgcagggcg cacacaatcg aaacggagtg gagattgtat
     3301 aagtgcgcac tttgtgattt gtcgcgcatt atgccagctc agtttttaaa ctaccagtga
     3361 cgcgctaaaa tgcgattttt catcgcgcac ggagttacat catgagcaga acgctcgaac
     3421 agaagattgc tgatgctgag gcgagattac agcgcctgaa ggcgaagagc cggagcctgg
     3481 acacagcgca gaaggttgtt gtgggtgcag cattgctggc aaaggtcaga aagccggagg
     3541 aggtgcagtt gcgtgcctgg ttactccagt tcctgaaggc agaggtgaca cgacaggctg
     3601 atgtgacgcg catactgccg ctgattaacg agctggaggc gctgccagga cagtgattgt
     3661 atcgtccgct cgccgcagcc ccagcgaccg agcgttagcg agcgagtggg cgaggaagcg
     3721 gaagtgaatc cgtcgccagg ttgagcacat acgcacggtt tttcggtagg tgatatgcgc
     3781 tgaacccgca tgaatactgg atttccacag ggttggaagg ggagtattcg gaacttgcaa
     3841 ttctgctttc ttgcaacaca ttgtttttat tggttttttg ttttcagcgc cccaagttat
     3901 ccacaggctc gccgttaagt ttttagatct tgtttttaag atcttgtttt taatacttgt
     3961 tttagggtca aaattcatta ataaaatcaa tatgttatag aggtatatgt ggaggaattt
     4021 aaggtaaagg tggaggaatt taaggtgttt aggtggagga atttaaggta aa
//
