LOCUS       CP114349                6200 bp    DNA     circular BCT 29-DEC-2022
DEFINITION  Escherichia coli strain 2022CK-00559 plasmid unnamed2, complete
            sequence.
ACCESSION   CP114349
VERSION     CP114349.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN31390131
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     2022CK-00559
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2022) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
REFERENCE   3  (bases 1 to 6200)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 47.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/14/2022 17:31:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,748
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,415
            CDSs (with protein)               :: 4,415
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 211
            CDSs (without protein)            :: 211
            Pseudo Genes (ambiguous residues) :: 0 of 211
            Pseudo Genes (frameshifted)       :: 74 of 211
            Pseudo Genes (incomplete)         :: 137 of 211
            Pseudo Genes (internal stop)      :: 48 of 211
            Pseudo Genes (multiple problems)  :: 45 of 211
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00559"
                     /isolation_source="Wound/Abscess"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA"
                     /collection_date="2022-04-05"
     gene            complement(join(5452..6200,1..55))
                     /gene="aph(3'')-Ib"
                     /locus_tag="OIX73_23695"
     ARG             complement(5456..6198)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="92.88"
     gene            complement(116..931)
                     /gene="sul2"
                     /locus_tag="OIX73_23700"
     ARG             complement(120..931)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2120..2269
                     /locus_tag="OIX73_23705"
     CDS             2120..2269
                     /locus_tag="OIX73_23705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WBD27768.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(3014..3454)
                     /locus_tag="OIX73_23710"
     CDS             complement(3014..3454)
                     /locus_tag="OIX73_23710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WBD27769.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="entry exclusion protein 1"
     gene            3672..4061
                     /locus_tag="OIX73_23715"
     CDS             3672..4061
                     /locus_tag="OIX73_23715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WBD27764.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            4105..4509
                     /locus_tag="OIX73_23720"
                     /pseudo=""
     CDS             4105..4509
                     /locus_tag="OIX73_23720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(4616..5452)
                     /locus_tag="OIX73_23725"
     CDS             complement(4616..5452)
                     /locus_tag="OIX73_23725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WBD27765.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     ARG             complement(4620..5452)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          1912..2085
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(1383..1988)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tgacaggcaa ccagtcagaa tgcgattcac caaaaaaaat attagttcga ttcaatggag
       61 gttccttcag ttttctgatg aagcgcgaat atagagaaat atcccgaatg tgcagttaac
      121 gaattcttgc ggtttctttc agcgccgcca ataccgccag cccgtcgcgc aaggggcgcg
      181 gctcgtgtgt gcggatgaag tcagctccac ctgcggcggc ggcaagctct gcagcgagtg
      241 tcgcggcccc gacatccccc ggaccacggc ctgtgagcgc gcgcagaaag gatttgcgcg
      301 aaacagacag aagcaccggc aaatcgaagc gcagccgcaa ttcatcgaac cgcgccagca
      361 ccgagagcga ggtttcggga gcagccccca gaaaaaaccc catgccggga tcaaggacaa
      421 ggcggttgcg tttgataccg gcacccgtca gcgccgcgat gcgcgcgtca aagaacgccg
      481 caatgtgatc catgatgtcg ccagcgggtg cctcgcgccg atctgcctgc ccgtcttgca
      541 ccgaatgcat aacgacgagt ttggcagatg atttcgccaa ttgcggatag aacgcagcgt
      601 ctggaaaacc gcgaatatca ttgagatagg ccacaccacg cgacaaggca taggcttgcg
      661 tcgcgggttg ataactgtcg agcgagacgg gaatgccatc tgccttgagc gcgtccagca
      721 ccggcgcgat acgcgcgatt tctgtgtcgg acgaaacagg cgcggcgtcg ggattgctgg
      781 atgccggacc gaggtcgatc acatctgccc cctcggccat cagcttacgc gcctgcgcaa
      841 tggctgcgtc tggcgccaga taccggcctc catcggagaa actgtccgag gttatgttga
      901 cgatgccgaa aatgatgagc gatttattca tgggggcttc tataataata ataatcgagc
      961 atgagtctca tacggatgct cgggtcgaaa gggaatcccc aggcgagtaa cctgtttgcg
     1021 gtgatccgtt tcatgatgat gtatctgtag cattttggaa ataaatatat catctctcaa
     1081 taagtagata tatttatctt ttttgcttct ttggtgctgc aagtcaccaa tacgagggaa
     1141 acctgttacg gagcggttta accgtaatca acagtgactg gtgtcatgtt cggaagatta
     1201 attgatagta atctacaaag gatcttcttg agatcctttt caatgaatcg taatctcttg
     1261 ctctgtaaac gaaaaaaccg cctggggagg cggtttgttc gaaggttaag tcagttgggg
     1321 aactgcttaa cccggtaaca gggttcacga gaccgccatt accaaaactg ttctttcagt
     1381 gtagccgcag tcaggcctct acttcaagac ctcttaatct atcttctcgt gcactcctgt
     1441 tactaatggc tgctgccagt ggcgttttgt cgtgtctttc cgggttggac tcaagttgat
     1501 agttaccgga caaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct
     1561 tggagcgaac tgccttcacg gaactgagtg tcaggcgtgg aatgagaaac cgcggccata
     1621 acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagctac
     1681 cagggggaaa cgcctgggat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc
     1741 gtcagatttt gcgatgcttg tcaggggggc ggagcctatg gaaaaacggc ttcgccgtgg
     1801 ccttatctct tctttgctaa gtgtcttcct ggtatcttcc aggaaatttc cgtccccgtc
     1861 cgtaagccgg taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg
     1921 aagcggaata tctcctgtat cgcatattct gctgacgcgc cggtgctgcc ttttttctcc
     1981 tgccacatga agcacttcac tgattttcgc aatcgtggca acatagtaag ccagtataca
     2041 ctccgctagc gctacatgac tggttcaggg ctgcgccccg aaacccgcta acaacctctc
     2101 agctactgga tatcacccca tgccatctga atatgatgaa caactggatt cgactgatac
     2161 gcagcttccc ggcgaatatg acctttttga tttaatggcg caggttactg aagagaacct
     2221 gcatgcccct gtggattttg ggtcgcctgt cggccgggaa gcgttatagc tttttcgctt
     2281 gcccgcgtac gtggtaatac tccggtatta ccttttattt ctttattttc atgtaattac
     2341 agtggatttt taatacccgg gtattactcg tgaaaacgaa atattccttt tttttctttt
     2401 aattcatgca gttgcgatga aaatcgcggc tgcgttaggt gtatgaccat tttgaggttg
     2461 aaaaataatc aaccatggcg ggtgatttac gcaggttaag gggagatcgt gacaaaaaaa
     2521 gcgttcctca ctgtatcgtt catgcctgca tgaacgggtt attcttcctg actacagaat
     2581 tacatcgctc cttcagttca tacatcactt ttacatcact tatacatcac ttttacatca
     2641 ttccttcagc tcatacgtca ctttcatcct ctgtgccatt tgtacatcac ccccttcctg
     2701 aacgtgctga cagcgggttt tccggctgcg ccggcaacgc ttcacggcca cgcaataaat
     2761 tgcgtccccg ttcaccgttt tcggatggga agaacgccgg aaacaggact taccccggct
     2821 gaatggtgtg aatttccgcg ctatgcactt gtgcgcatac tcatgcatgc cgtaaaaaca
     2881 gagcctgcgc gtttctggcg ggttttcggg tggtttgttg cctgttttac cggtttcccg
     2941 tcagaaacgc cctgagggcg tctgagatgg tgcacgcaac gaatgttatg gtaaatcaga
     3001 gtcacctgca ctgttactct gaaccggagc gaaacagccg cgaccagaat ccccgctttt
     3061 tttcctgtgc cagcgcgtca gccagacggc taatttcggc ctgtagttgt tccctctcct
     3121 catttcgcag gcggttttct gcttcccgcg ccatcttgtc ttcaagcatt agggtaacag
     3181 tctgctgcag agcgtccagt ttctgctgaa tgacgtctag ctgagcattt tccgcctggc
     3241 taacgacaga tttggagtgt ccctccttgt gcctttttgg tgtctcagaa gtgcgcagct
     3301 cgccataggc ccgaatcagt tcactggtct cgaactcccg gcgatcattt tttcctgtgc
     3361 gatagctgac aaggccttta gccatgtcac gatataattg cgagcgcgac tttcctgtta
     3421 atttcagtgt ctcttgaact gtgtgccatg ccataaaagt gacagtgtct cctgtgtgta
     3481 ccgtattgta acagcagaac atattactgt tttaacacaa aaggtctgag gctgcccgtg
     3541 aggcgtgaaa aatacacaaa aaacctgttg acggtgtggt tttgacgggc gtagtatttc
     3601 gaatactgta tataaacaca ttgttataaa tacagttttt gctgttttta aaagtcaaag
     3661 aggataattt tatgagtaca gagttaaata gcggtactgt cggtggtttt ggtcacatgg
     3721 gaccaaatga cccctacaat gatggtcata gcggctctgg aagttcggga ggtagtttta
     3781 acggtggtgg taatggtggc catgataata gtagtggttc atcttatact gcatcctttg
     3841 gtctcatcac tggccgacac ccgataactg tcccaggtac gaagaatatt aattctgtcg
     3901 atctttctgc tgtcattaat aacattatac atcatgaagg tgcacgcaat aacatgtacc
     3961 tagatactgc gaacaatgta agagtgggag tgacgggcac tggctggcaa tgtctagcaa
     4021 cggcaggcat ttcggctgag ggtaaaagaa ctttccgcta agcgatagac tgtatgtaaa
     4081 cacagtattg caaggacgcg gaacatgcct catgtggcgg ccaggacggc cagccgggat
     4141 cgggatactg gtcgttacca gagccaccga cccgagcaaa cccttctcta tcagatcgtt
     4201 gacgagtatt acccggcatt cgctgcgctt atggcagagc agggaaagga attgccgggc
     4261 tatgtgcaac gggaatttga agaatttctc caatgcgggc ggctggagca tggctttcta
     4321 cgggttcgct gcgagtcttg ccacgccgag cacctggtcg ctttcagctg taagcgtcgc
     4381 ggtttctgcc cgagctgtgg ggcgcggcgg atggccgaaa gtgccgcctt gctggttgat
     4441 gaagtactgc ctgaacaacc catgcgtcag tgggtgttga gcttcccgtt tcagctgcgt
     4501 ttcctgtttg gggtcgtttg cgggaagggg cggaatccta cgctaaggct ttggccagcg
     4561 atattctccg gtgagattga tgtgttccca ggggatagga gaagtcgctt gatatctagt
     4621 atgacgtctg tcgcacctgc ttgatcgcgg ccgcgatagc tagatcgcgt tgctcctctt
     4681 ctccatccgc gttccaagct gcggaaaggc acccataagc gtacgcctgg tcgagcaggc
     4741 gacgcggatc gacgtccagc gcacgagaga atgcgtccgc catctgtgca atgcgtctag
     4801 gatcgagaca aaggtcgtct ctgtcagccg gatcgtagaa catattggcg gcgccaaagc
     4861 ccacttcacc gaccagaccg acgggatcta tcaccagcca gccgcgactg gagaacatga
     4921 tgttttcatg atgcagatcg ccatgtagcc cacgcagttc cgaggcattg ctcatcattt
     4981 gatcggctat aatcgccgcg tggacgtagt cagtttgaca acctgcgttt tgatcatcgc
     5041 gcgcccgctg aaacaaagct gcaaagcgat cccggatcgg gagaagggca gaaggcaggg
     5101 gttcctcaga tgcggcatac agcttcgcca ttagttccgc tgcaatttcg gtcgcctggt
     5161 agtcgccgtg ctcggcaacg atgtgagaga gcattcgctc cccggcatat tcgagcaaca
     5221 tcagattgtt ctcacgaccg agcaaccgga ctgctcccct cccattgcgc cataccagat
     5281 agtcggcccc gcgcagttca tcagcaatgt cttctatagg tttcaatccc ttgacgattg
     5341 caggagtccc gtctggcaat gaaactttcc aaacgaggct ggaaaaggtg tccgcaatga
     5401 gaacaggttg cgaaacgtgc caatgagcag gaaaaacagg cggcatgaac atcaacccca
     5461 agtcagaggg tccaatcgca gatagaaggc aaggcgttcg cggtcggggg cttcgatccc
     5521 caatacattg aataggacag cgaaggcgcg ctctgcttca tctggcgctg cccagttctc
     5581 ttcggcgtta gcaatcatga gtgccaaatc ggcatagcga tctgctgttc cgagccgccc
     5641 aaggtcgatc agacccgtgc attgaagagt tttagggtcc accatgaagt tcggcatgca
     5701 gggatcacca tggcaaacaa ccatatcggt gcgctcttgg tcgagccgca ccggtagctc
     5761 tcgttcgaca cgagccaaaa gatcgagctg cggcgtactc ttgtcctcgt ccggtaagaa
     5821 gtcgggattg acggcattgc gggacaccac atcaacggcg cgtccgaaca ttcgcgacag
     5881 cctgcgctca aacggacatt gatcaaccga taggctgtga acagcgccaa gttgctgccc
     5941 cattgacggc cacgctttga gcaaatccgc tccagacaga tcagccgccg gtactcccgg
     6001 aattgccgtt atcaccaagc atgcaccctc ctgttcctcc tgccagttga tgacctcggg
     6061 gcaagccaca cctcgacctt tgagccaaat gaggcggtca cgctctccag cgagctcacc
     6121 gcggcgggaa gcaggtgcga ttttcgcgaa ggcatgcccg tcaccacgtc gaaaaacaaa
     6181 atcaccagat tctccgcctc
//
