LOCUS       CP114339                4072 bp    DNA     circular BCT 29-DEC-2022
DEFINITION  Escherichia coli strain 2022CK-00554 plasmid unnamed2, complete
            sequence.
ACCESSION   CP114339
VERSION     CP114339.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN31261076
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     2022CK-00554
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
REFERENCE   3  (bases 1 to 4072)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 53.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/14/2022 17:57:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,623
            Genes (coding)                    :: 4,407
            CDSs (with protein)               :: 4,407
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 74 of 216
            Pseudo Genes (incomplete)         :: 141 of 216
            Pseudo Genes (internal stop)      :: 49 of 216
            Pseudo Genes (multiple problems)  :: 45 of 216
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00554"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA"
                     /collection_date="2022-09-05"
     gene            complement(join(4003..4072,1..2090))
                     /locus_tag="OGU26_23710"
     CDS             complement(join(4003..4072,1..2090))
                     /locus_tag="OGU26_23710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WBD41608.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            2269..2523
                     /gene="mobC"
                     /locus_tag="OGU26_23715"
     CDS             2269..2523
                     /gene="mobC"
                     /locus_tag="OGU26_23715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WBD41606.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            3055..3747
                     /locus_tag="OGU26_23720"
     CDS             3055..3747
                     /locus_tag="OGU26_23720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WBD41607.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(1733..2091)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          2092..2268
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          3056..3747
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..2177)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.448"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 tcatcccagg tacgattgaa acccagagag cggaacagat gatccggctg tttttctggt
       61 gtgttgacgg cagcaaaaag cggtgcctca cgggttttca gatgaaactc cataagttcc
      121 ggcgcaggtg atatgtggct gtctgcatcg tgatttacgg caacaccata ccctgcatac
      181 cgtaacccct tatcggtcag tgcactgatg accagctggc tgttatcggc tgagggaacg
      241 gcaaacgcgg gatacagcgt accattaagc cggacaacac cgcccagaga gtcatccacg
      301 cccatgacca gcaaaagctg aagctggcgg gtttcccggt cgacagaact cattaccggc
      361 atcagcgtcg gtagcagacg tccctgcgta aaatccacct tccttgctgc cagtgatggc
      421 atggctggct ggcggtcttc tccaagacag aaagactccg gccatgtctt cggctggcgg
      481 agattgcggg tatattgcgg ccatgtctgg caaacctgcg tcagaagttg ctcaaaggtc
      541 gcagaggaat aaacacgttg cagtggtgga tgttgaccga tttcgcttcg gtcacagata
      601 taaccactcc tgtcacgcat taaatcaaca cgctcaaaga ttctggtgcg gtatttctcc
      661 agctcgttcc cccagagcgt gtattctccg ttccggttgt gaagcagaag gtaacaggtc
      721 atttccccgt taccaggcat gggggcctcg ccataccccg taacgactgc gctcagtacc
      781 cctgaatcca taacggcatt gtcatttttc ctgagctgtt ccgctcgtcg ctccgcgcga
      841 ctacgttctg tatcctcctg ctccttccgg cgcaattcct cttcacgttt aagcaactgg
      901 actctgagag ctgcaaggcc ggatgccccc cactgctgcc actgtttttc aaatgccgtc
      961 tgactcgcgg gaatgtcctg tttctccacc ttccggtaat gctgtcgcaa tttttccatt
     1021 tgctggctca gcgactggat ttttccctgt tcgctacggt attccggtgc cgcccattgc
     1081 atcaggggaa tacgggaaaa accggacgaa aacggcgatg attttttcag gctggcagca
     1141 tggctcatcg cttcctttcg ttcggtgttc agctcttccc agcgtctgcg cgtcgcctgc
     1201 atatctgcca tcatcgacat ggcattactg gtgagtttcc atgccgcatt actgtccttc
     1261 ggcaacgctt cgatgatgtc acgtagtgtg tacgaagttg cgttgtcagg aagcacgata
     1321 ttcagaaggt cagccacacg gcgggcaggc tctgtcagcc cgctgttcct gagttcagtg
     1381 cgggaaatct gtatttccag gttaagcctg ttaatcagac gattctgttc gccgcgttct
     1441 gtttcgatac cgcgtgtttc catcgcacag gcagactttc cgaggtgaat accaggcgtt
     1501 ttctcaagcc cctgtcgctc gtatgaacga tggtctatct cttcctgata accggcgttc
     1561 gccagtgctc tgtttgcatg gtcagcccat gatgcacgcc acgtctcggc atgtgtccgg
     1621 tcgttccagt ccctgacctt tccgccgaac ccagcaggtc cgacagccct cgtggtcagc
     1681 atgatgtgcg catggggatt tgttttatcc atgtgatgga acgccacatc agcaatcatg
     1741 ccctgactga caaagttttc tctgacaaag gcgagaacgg tttcccgtgc ggcatcctgc
     1801 ggcagttcac gggggatagc gagttcaatt tcccgcgcaa gttgtgagtt cttccggcat
     1861 tcggccttct caacggcatt ccagagttcg gcacggttcg tacaccaggc aggggctgtc
     1921 gcgggggtca gaatcacagc ttcagccaca ccgcttttgc gggtgtagtc gtgagtcagt
     1981 ccggtacgct catcaagcat cagtgaaccg gaacgatagg cagcggaagc tacggcgctg
     2041 tagccgctgc ttctggagat aattttcatg ctgagatgat aaatcgccat ttctgccgta
     2101 atgccttttt gtagttttct ttcagccccg cagggcgcac acaatcgaaa cggagtggag
     2161 attgtataag tgcgcacttt gtgatttgtc gcgcattatg ccagctcagt ttttaaacta
     2221 ccagtgacgc gctaaaatgc gatttttcat cgcgcacgga gttacatcat gagcagaacg
     2281 ctcgaacaga agattgctga tgctgaggcg agattacagc gcctgaaggc gaagagccgg
     2341 agcctggaca cagcgcagaa ggttgttgtg ggtgcagcat tgctggcaaa ggtcagaaag
     2401 ccggaggagg tgcagttgcg tgcctggtta ctccagttcc tgaaggcaga ggtgacacga
     2461 caggctgatg tgacgcgcat actgccgctg attaacgagc tggaggcgct gccaggacag
     2521 tgattgtatc gtccgctcgc cgcagcccca gcgaccgagc gttagcgagc gagtgggcga
     2581 ggaagcggaa gtgaatccgt cgccaggttg agcacatacg cacggttttt cggtaggtga
     2641 tatgcgctga acccgcatga atactggatt tccacagggt tggaagggga gtattcggaa
     2701 cttgcaattc tgctttcttg caacacattg tttttattgg ttttttgttt tcagcgcccc
     2761 aagttatcca caggctcgcc gttaagtttt tagatcttgt ttttaagatc ttgtttttaa
     2821 tacttgtttt agggtcaaaa ttcattaata aaatcaatat gttatagagg tatatgtgga
     2881 ggaatttaag gtaaaggtgg aggaatttaa ggtgtttagg tggaggaatt taaggtaaag
     2941 gtggaggaat ttaaggtgtt attcacagaa atgtgaggat ataaaaaaaa atattccaca
     3001 tttcttattt gtggggcaca ttaaagcatc accacatttg aacgggatct ttgtatgtcg
     3061 gacttgattg cctacaagtc gaatgctttg gttgaggcca gctacaagtt gaccctacag
     3121 gaacaacgtt ttctgttgct gtgtatcagt cgattgaaat ctggtgctga tgctgaattg
     3181 caaaagacaa tgaccattac cgctgcggaa tattttgatt cattccctga catggggcgg
     3241 aaaaatgccg aggttcagtt acaggaagcg atcgatcgtt tatgggacag gtcgatcatt
     3301 ctcaagaatg acgagaagcg tgaggagttc cgctggatcc agtatcgggc acagtacgct
     3361 aaaggtgagg ccaaggctcg aataaccttt tctgacgcgg taatgcctta tctgacgcaa
     3421 ctgaaggggc aattcacgcg cgttgttata aaaaacatat caggtttatc aagtagttac
     3481 agtataagaa tatacgagtt cctccagcaa ttccgcagta cgggagagag aattattgct
     3541 cttaatgact ttcgttcaat gttagggatt gaaaataaat acaaacaatt cagagactta
     3601 aataaaatac taataaaacc ctgtgttgac gaactgaaca aaaaaagcga cctggctgta
     3661 acggttgaga cgatcaaaaa ggggcgcacg gttgtagccc tgcattttcg cttcaaggaa
     3721 gacaaacaga tcaagatgac gatctaaaat cgattttaag gcacctaaaa cgctccctgc
     3781 tgcgtttttt ttgtgttagt agtgcgaagg tactattgaa gtgaaaaatt gcgtcaaaag
     3841 cgctctaatg cagcgaaaaa agaaccccca atttttgggg gcggaaaaac aaaactatgc
     3901 ttgcattatg tgtgtgcttc ttgcattcat ccctgtcgat gaacatagaa gaacatcgac
     3961 gaacataaca cgatgttaaa caaaacaaaa tattgttcac attcactgtg accaaccgcg
     4021 atcatgtctc ctctcggtgg tgtgtgttct tgcatcttcc tcgcgccgcc at
//
