LOCUS       CP113402                2362 bp    DNA     circular ENV 06-DEC-2022
DEFINITION  MAG: Staphylococcus haemolyticus isolate 1710 plasmid unnamed3,
            complete sequence.
ACCESSION   CP113402
VERSION     CP113402.1
DBLINK      BioProject: PRJNA865942
            BioSample: SAMN30148945
KEYWORDS    ENV; Metagenome Assembled Genome; MAG.
SOURCE      Staphylococcus haemolyticus (mixed culture metagenome)
  ORGANISM  Staphylococcus haemolyticus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Identification and pathogenic (toxigenic) potential of
            Staphylococcus haemolyticus and Bacillus paranthracis consortium
            isolated from bovine mastitis in Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) Microbiology Laboratory, Federal Research
            Center for Animal Husbandry named after Academy Member L.K. Ernst,
            Dubrovitsy 60, Podolsk Municipal District, Moscow Region 142132,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/30/2022 11:10:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,595
            CDSs (total)                      :: 2,510
            Genes (coding)                    :: 2,467
            CDSs (with protein)               :: 2,467
            Genes (RNA)                       :: 85
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 19 of 43
            Pseudo Genes (incomplete)         :: 24 of 43
            Pseudo Genes (internal stop)      :: 12 of 43
            Pseudo Genes (multiple problems)  :: 10 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from staphylococcus haemolyticus.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Staphylococcus haemolyticus"
                     /mol_type="genomic DNA"
                     /isolate="1710"
                     /isolation_source="milk"
                     /host="bovine"
                     /db_xref="taxon:1283"
                     /environmental_sample=""
                     /plasmid="unnamed3"
                     /geo_loc_name="Russia: Mordovia region"
                     /collection_date="2020"
                     /metagenome_source="mixed culture metagenome"
                     /note="metagenomic"
     gene            320..1054
                     /gene="erm(C)"
                     /locus_tag="NRZ55_12970"
     ARG             321..1051
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1502..1978
                     /locus_tag="NRZ55_12975"
     CDS             1502..1978
                     /locus_tag="NRZ55_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WAI21730.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     ARG             321..1054
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.59"
                     /coverage="100.0"
     Typing          1503..1988
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatagttttc tcggcataaa gccatgctct gacgcttaaa tgcactaatg ccttaaaaaa
       61 acattaaagt ctaacacact agacttattt acttcgtaat taagtcgtta aaccgtgtgc
      121 tctacgacca aaagtataaa acctttaaga actttctttt ttcttgtaaa aaaagaaact
      181 agataaatct ctcatatctt ttattcaata atcgcatcag attgcagtat aaatttaacg
      241 atcactcatc atgttcatat ttatcagagc tcgtgctata attatactaa ttttataagg
      301 aggaaaaaag agggttataa tgaacgagaa aaatataaaa cacagtcaaa actttattac
      361 ttcaaaacat aatatagata aaataatgac aaatataaga ttaaatgaac atgataatat
      421 ctttgaaatc ggctcaggaa aagggcattt tacccttgaa ttagtacaga ggtgtaattt
      481 cgtaactgcc attgaaatag accataaatt atgcaaaact acagaaaata aacttgttga
      541 tcacgataat ttccaagttt taaacaagga tatattgcag tttaaatttc ctaaaaacca
      601 atcctataaa atatttggta atatacctta taacataagt acggatataa tacgcaaaat
      661 tgtttttgat agtatagctg atgagattta tttaatcgtg gaatacgggt ttgctaaaag
      721 attattaaat acaaaacgct cattggcatt atttttaatg gcagaagttg atatttctat
      781 attaagtatg gttccaagag aatattttca tcctaaacct aaagtgaata gctcacttat
      841 cagattaaat agaaaaaaat caagaatatc acacaaagat aaacagaagt ataattattt
      901 cgttatgaaa tgggttaaca aagaatacaa gaaaatattt acaaaaaatc aatttaacaa
      961 ttccttaaaa catgcaggaa ttgacgattt aaacaatatt agctttgaac aattcttatc
     1021 tcttttcaat agctataaat tatttaataa gtaagttaag ggatgcagtt tatgcatccc
     1081 ttaacttgtt tttcgtgtac ctattttttg tgaatcgatt atgtcttttg cgcattcact
     1141 tcttttctat ataaatatga gcgaagcgaa taagcgtcgg aaaagcagca aaaagtttcc
     1201 tttttgctgt tggagcatgg gggttcaggg ggtgcagtat ctgacgtcaa tgccgagcga
     1261 aagcgagccg aagggtagca tttacgttag ataaccccct gatatgctcc gacgctttat
     1321 atagaaaaga agattcaact aggtaaaatc ttaatatagg ttgagatgat aaggtttata
     1381 aggaatttgt ttgttctaat ttttcactca ttttgttcta atttctttta acaaatgttc
     1441 tttttttttt agaacagtta tgatatagtt agaatagttt aaaataagga gtgagaaaaa
     1501 gatgaaagaa agatatggaa cagtctataa aggctctcag aggctcatag acgaagaaag
     1561 tggagaagtc atagaggtag acaagttata ccgtaaacaa acgtctggta acttcgtaaa
     1621 ggcatatata gtgcaattaa taagtatgtt agatatgatt ggcggaaaaa aacttaaaat
     1681 cgttaactat atcctagata atgtccactt aagtaacaat acaatgatag ctacaacaag
     1741 agaaatagca aaagctacag gaacaagtct acaaacagta ataacaacac ttaaaatctt
     1801 agaagaagga aatattataa aaagaaaaac tggagtatta atgttaaacc ctgaactact
     1861 aatgagaggc gacgaccaaa aacaaaaata cctcttactc gaatttggga actttgagca
     1921 agaggcaaat gaaaaacaag aaaatgcatt atctgattat tattctttca aggactagta
     1981 taacataaaa tcgtctacaa atagacaaaa aacctgcacg cttaatgtag atcaaaagct
     2041 taacgcaaat gaaatagatt gacctcccaa taacaccacg tagttattgg gaggtcaatc
     2101 tatgaaatgc gattaagctt tttctaattc acataagcgt gcaggtttaa agtacataaa
     2161 aaatataatg aaaaaaagca tcattatact aacgttatac caacattata ctctcattat
     2221 actaattgct tattccaatt tcctattggt tggaaccaac aggcgttagt gtgttgttga
     2281 gttggtactt tcatgggatt aatcccatga aacccccaac caactcgcca aagctttggc
     2341 taacacacac gccattccaa cc
//
