LOCUS       CP113401                3048 bp    DNA     circular ENV 06-DEC-2022
DEFINITION  MAG: Staphylococcus haemolyticus isolate 1710 plasmid unnamed2,
            complete sequence.
ACCESSION   CP113401
VERSION     CP113401.1
DBLINK      BioProject: PRJNA865942
            BioSample: SAMN30148945
KEYWORDS    ENV; Metagenome Assembled Genome; MAG.
SOURCE      Staphylococcus haemolyticus (mixed culture metagenome)
  ORGANISM  Staphylococcus haemolyticus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Identification and pathogenic (toxigenic) potential of
            Staphylococcus haemolyticus and Bacillus paranthracis consortium
            isolated from bovine mastitis in Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) Microbiology Laboratory, Federal Research
            Center for Animal Husbandry named after Academy Member L.K. Ernst,
            Dubrovitsy 60, Podolsk Municipal District, Moscow Region 142132,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/30/2022 11:10:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,595
            CDSs (total)                      :: 2,510
            Genes (coding)                    :: 2,467
            CDSs (with protein)               :: 2,467
            Genes (RNA)                       :: 85
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 19 of 43
            Pseudo Genes (incomplete)         :: 24 of 43
            Pseudo Genes (internal stop)      :: 12 of 43
            Pseudo Genes (multiple problems)  :: 10 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from staphylococcus haemolyticus.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Staphylococcus haemolyticus"
                     /mol_type="genomic DNA"
                     /isolate="1710"
                     /isolation_source="milk"
                     /host="bovine"
                     /db_xref="taxon:1283"
                     /environmental_sample=""
                     /plasmid="unnamed2"
                     /geo_loc_name="Russia: Mordovia region"
                     /collection_date="2020"
                     /metagenome_source="mixed culture metagenome"
                     /note="metagenomic"
     gene            join(2763..3048,1..536)
                     /locus_tag="NRZ55_12960"
     CDS             join(2763..3048,1..536)
                     /locus_tag="NRZ55_12960"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="WAI21728.1"
                     /translation="MFFKCSICDEKLKRGYKTLACENNHTFDIAKEGYSNLISGHKKSK
                     YDKKLFIARRELNDSSNLYSILISKIIEIIKKYEIKTKNNVIDVGTGEGYILNEISKTF
                     LGENEFLGIDIAKEGVKKAAKSYKNINWLVADLADIPVMNGNVDFLINVLSPSNYDEFK
                     RVLDKEGKLIKIIPNSDYLKEIRNIVFSNHKNKDYDNSEVIDLFEANFNKIEMIDISYK
                     VKLNKEELNKLLDMTPLTWNLSDVEKEGIIKKNLSEITMSFKVLVGGFKTS"
                     /name="methyltransferase domain-containing protein"
     gene            719..1723
                     /locus_tag="NRZ55_12965"
     CDS             719..1723
                     /locus_tag="NRZ55_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020368559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WAI21729.1"
                     /translation="MQYNTTKCIDENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNVLEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EEVVKEKPTARWLFLTLSTKNAIDGDHLEQSLKHMSKAFNKLKMYTKVKKNLVGFLRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQVEWVDLWQKALQVNYRPVANIKAI
                     KPNQKGDKDIQAAIKETSKYSVKSSDFLTDDDEKNQEIVNDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDAEDGNLINTSDEDKTTDEEEKAHSITAIWNFEKQNYYLKDLKR"
                     /name="protein rep"
     Typing          720..1723
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.806"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttttaaatga aattagtaaa acatttcttg gtgaaaatga attcttaggt atagacattg
       61 caaaagaagg tgtaaaaaaa gcagcaaaat catataaaaa tataaattgg ttggttgctg
      121 atctagctga tataccagtt atgaatggga atgtagactt tttaattaat gtgcttagcc
      181 cttctaatta tgatgagttt aaaagagttc tagataaaga ggggaaattg ataaaaataa
      241 ttcctaatag tgattattta aaagaaataa ggaatatagt ctttagtaat cataaaaata
      301 aggattatga taattctgag gttatagatt tatttgaagc taattttaat aaaattgaaa
      361 tgatagacat tagttataag gttaaattaa ataaagaaga attgaataaa ttacttgata
      421 tgacgccttt aacgtggaat ttaagtgacg tagaaaaaga aggaataatt aagaaaaact
      481 taagtgaaat tacgatgagt tttaaggttt tggttggtgg ttttaaaacg agttgaaaac
      541 gagtttcttc ttgtcttgat actatataga aataactcaa agatataaaa agttgcttta
      601 tcgttggtat gacagtgttt ttatgtattt ttgtgcgtga aagaaaaaat tgacataaaa
      661 aagctccagt aatatgatta agttacgacg cttaaatcaa tactggaggt ctttttatat
      721 gcaatataat actactaaat gtatagacga aaatcaagat aataaaacat taaaagatat
      781 gacgaaaagt gggaaacaac gcccatggag agaaaagaag atagataatg taagttatgc
      841 agatatactg gaaatcttaa aaattaaaaa ggcttttaac gtaaaacaat gtggtaacgt
      901 cttagaattc aagccaactg atgaaggtta tttgaaatta cataagacat ggttttgtaa
      961 gtcaaaatta tgcccagttt gtaattggag gcgtgctatg aaaaatagtt atcaagctca
     1021 aaaagtgatt gaagaagtgg ttaaagaaaa gccaactgct cgttggttat ttcttacttt
     1081 atcgactaaa aatgcaatag acggtgacca cttagaacaa agtttaaaac acatgtctaa
     1141 ggcttttaat aaattaaaga tgtatacaaa agttaaaaag aatttggtgg gttttttacg
     1201 ctcaactgaa gtaacagtta ataaaaatga cggtagttat aatcaacata tgcacgtttt
     1261 attgtgtgtt gaaaatgcgt atttcagaaa aaaagaaaac tacattacgc aagttgaatg
     1321 ggttgattta tggcaaaaag cattacaggt taattatcga ccagtagcaa atattaaagc
     1381 gatcaaacca aatcaaaaag gcgataaaga tattcaagca gctatcaaag aaacctctaa
     1441 atattcagtt aagtcatctg attttttaac tgatgatgac gaaaaaaatc aagaaatcgt
     1501 gaatgatttg gaaaaaggtt tataccgaaa acgtatgttg agttatggtg gattgcttaa
     1561 acaaaaacat aaaattttaa atttagacga tgccgaagat ggcaatttga ttaatacaag
     1621 tgacgaagat aaaacaacag acgaagaaga aaaagcacat tcaattacgg caatttggaa
     1681 ttttgaaaaa caaaattatt acttaaaaga tttgaaacgt tagcttaaaa gctagcgttt
     1741 tttttatgtc tttttgcccc tgcgggaact atagcgttca accggcatgg ttgcccaatt
     1801 ttcatgttaa tttttaaatt tgcatgtaac tgggcagtgt ttattttttc ttgcccattt
     1861 tgctaacttt taagtttgca tataaatggg cagtgtctaa aaaattcccc actggtcatg
     1921 aacaaaaaaa tgagctaaac aatttaactt tttaagttat ataaatttta gggagttcca
     1981 atcaaattag aaaaatatat aaaagagcta tatcgttttt ctggcttatg tttaaaaagt
     2041 acaagtgatt gtatttaaaa tgactttagc aatttaagta atatgtcttt tgaatacaag
     2101 ttaaatgtca gaaagatgta cccttatttt gctcatattt gcgttttaag aaatgaatat
     2161 gattatacgt atataaaact atataaattt agagtttgct ctaaataccc ccttaaaatt
     2221 caaaataaag gcatttaaaa attaagaaaa taaaaaagct actagcaaaa aactagtagc
     2281 ttaaatctta gttaaatttc tatgtagtat taatttttat atctacgcta caacgtcgat
     2341 tctagttcgt gcagtattaa tttttatatc tgcgccacaa cgccgattcc ttaaaactaa
     2401 gataaaaatt gacgaaggtc gattatggtt ttttacgcgt tctttttaga acgcataagt
     2461 gcgcccttac gggatttaac tagattataa cgacgaaaaa tagacctgta aagcaataac
     2521 agttgggtct actttgtcta ctgggcagac aagaaaagaa cacgcattta tgccgagaaa
     2581 atttattgat gttgagaaga acccttaact aaacttgcag acgaatgtcg gcatagcgtg
     2641 agctattaag ccgaccattc gacaagtttt gggattgtta agggttccga ggctcaacgt
     2701 caataaagca attggaataa agcaactttg ttatttataa ttatttgatt ggaggtgtga
     2761 atatgttttt taagtgttct atatgtgatg aaaaattaaa gagaggttat aaaactttag
     2821 catgtgagaa taaccatact tttgatattg caaaagaggg ttattcgaat ttaatttcag
     2881 ggcataaaaa aagtaaatac gataaaaaat tgtttattgc tagaagagaa ttgaatgata
     2941 gtagcaattt gtattcgata ttaatttcaa aaatcataga aattattaaa aaatatgaga
     3001 ttaaaactaa aaataatgtg atagatgttg gtactggtga gggttata
//
