LOCUS       CP113400                6539 bp    DNA     circular ENV 06-DEC-2022
DEFINITION  MAG: Staphylococcus haemolyticus isolate 1710 plasmid unnamed1,
            complete sequence.
ACCESSION   CP113400
VERSION     CP113400.1
DBLINK      BioProject: PRJNA865942
            BioSample: SAMN30148945
KEYWORDS    ENV; Metagenome Assembled Genome; MAG.
SOURCE      Staphylococcus haemolyticus (mixed culture metagenome)
  ORGANISM  Staphylococcus haemolyticus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Identification and pathogenic (toxigenic) potential of
            Staphylococcus haemolyticus and Bacillus paranthracis consortium
            isolated from bovine mastitis in Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) Microbiology Laboratory, Federal Research
            Center for Animal Husbandry named after Academy Member L.K. Ernst,
            Dubrovitsy 60, Podolsk Municipal District, Moscow Region 142132,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/30/2022 11:10:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,595
            CDSs (total)                      :: 2,510
            Genes (coding)                    :: 2,467
            CDSs (with protein)               :: 2,467
            Genes (RNA)                       :: 85
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 19 of 43
            Pseudo Genes (incomplete)         :: 24 of 43
            Pseudo Genes (internal stop)      :: 12 of 43
            Pseudo Genes (multiple problems)  :: 10 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from staphylococcus haemolyticus.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Staphylococcus haemolyticus"
                     /mol_type="genomic DNA"
                     /isolate="1710"
                     /isolation_source="milk"
                     /host="bovine"
                     /db_xref="taxon:1283"
                     /environmental_sample=""
                     /plasmid="unnamed1"
                     /geo_loc_name="Russia: Mordovia region"
                     /collection_date="2020"
                     /metagenome_source="mixed culture metagenome"
                     /note="metagenomic"
     gene            join(6084..6539,1..984)
                     /gene="aph(2'')-Ia"
                     /locus_tag="NRZ55_12935"
     CDS             join(6084..6539,1..984)
                     /gene="aph(2'')-Ia"
                     /locus_tag="NRZ55_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="WAI21725.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLES
                     LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFIGE
                     PNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLPEHE
                     LHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNE
                     YIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTF
                     LTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIY
                     NDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGI
                     IDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIK
                     NIKQEFIENGRKEIYKRTYKD"
                     /name="aph(2'')-Ia"
     gene            1183..2559
                     /gene="tet(L)"
                     /locus_tag="NRZ55_12940"
     ARG             1184..2556
                     /name="tet(L)"
                     /gene="tet(L)"
                     /gene_name="tetracycline efflux MFS transporter Tet(L)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3123..4385
                     /locus_tag="NRZ55_12945"
     CDS             3123..4385
                     /locus_tag="NRZ55_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WAI21727.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYD
                     LKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFE
                     ESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKF
                     PEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVK
                     SDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDND
                     RLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSK
                     ISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNE
                     FELTHNREVKKERSRDQGMSL"
                     /name="plasmid recombination protein"
     gene            4629..5612
                     /locus_tag="NRZ55_12950"
     CDS             4629..5612
                     /locus_tag="NRZ55_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WAI21723.1"
                     /translation="MCNNNSINSDEKSSVLIDKTKSGKIRPWREKKMANVDYFELLHIL
                     EFKKAERVKDCAEVLEYKVSYETGEKTLYRVWFCKSRLCPMCNWRRAMKHGIQSQKVVA
                     EVIKQKPTVRWLFLTLTVRNVYDGTELNKSLSDMSKGFNRMMKYKKINKNLVGFMRATE
                     VTVNNIDNSYNQHMHVLVCVESTYFYNTENYVNQKQWIQFWKRAMKLDYDPNVKVQMIR
                     PKNKHKSDIKSAIDETAKYPVKDIDFTTNDEEKNLKRLSDLEEGLHRKRLISYGGLLKE
                     IHKKLNLDDAEDGDLIHTDDEEKADEDGFSIIAMWNWERQNYFIKE"
                     /name="protein rep"
     gene            5691..6083
                     /locus_tag="NRZ55_12955"
     CDS             5691..6083
                     /locus_tag="NRZ55_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WAI21724.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYT
                     LLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACH
                     VYEKLGGKNDYKDEIVYVYDYEKGDK"
                     /name="GNAT family N-acetyltransferase"
     ARG             6085..6539
                     /name="AAC(6')-Ie-APH(2'')-Ia bifunctional protein"
                     /gene="AAC(6')-Ie-APH(2'')-Ia bifunctional protein"
                     /gene_name="aminoglycoside bifunctional resistance protein"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="31.73"
     ARG             2..984
                     /name="AAC(6')-Ie-APH(2'')-Ia bifunctional protein"
                     /gene="AAC(6')-Ie-APH(2'')-Ia bifunctional protein"
                     /gene_name="aminoglycoside bifunctional resistance protein"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="68.27"
     Typing          4630..5611
                     /name="rep_cluster_2104"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.727"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3124..4382
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.19"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttagaatta ttgaagattt gccagaacat gaattacacg agggcaaaaa agaagattgt
       61 tatttaatgg aatatagata tgatgataat gccacaaatg ttaaggcaat gaaatattta
      121 attgagcatt actttgataa tttcaaagta gatagtattg aaataatcgg tagtggttat
      181 gatagtgtgg catatttagt taataatgaa tacattttta aaacaaaatt tagtactaat
      241 aagaaaaaag gttatgcaaa agaaaaagca atatataatt ttttaaatac aaatttagaa
      301 actaatgtaa aaattcctaa tattgaatat tcgtatatta gtgatgaatt atctatacta
      361 ggttataaag aaattaaagg aactttttta acaccagaaa tttattctac tatgtcagaa
      421 gaagaacaaa atttgttaaa acgagatatt gccagttttt taagacaaat gcacggttta
      481 gattatacag atattagtga atgtactatt gataataaac aaaatgtatt agaagagtat
      541 atattgttgc gtgaaactat ttataatgat ttaactgata tagaaaaaga ttatatagaa
      601 agttttatgg aaagactaaa tgcaacaaca gtttttgagg gtaaaaagtg tttatgccat
      661 aatgatttta gttgtaatca tctattgtta gatggcaata atagattaac tggaataatt
      721 gattttggag attctggaat tatagatgaa tattgtgatt ttatatactt acttgaagat
      781 agtgaagaag aaataggaac aaattttgga gaagatatat taagaatgta tggaaatata
      841 gatattgaga aagcaaaaga atatcaagat atagttgaag aatattatcc tattgaaact
      901 attgtttatg gaattaaaaa tattaaacag gaatttatcg aaaatggtag aaaagaaatt
      961 tataaaagga cttataaaga ttgattatat aatatatgaa aagctattat aaaagacatt
     1021 agtattaaat agtttaaaaa aatgaaaaat aataaaggtt ttaatcaagt ttaggaggaa
     1081 ttaattatga agtgtaatga atgtaacagg gttcaattaa aagagggaag cgtatcatta
     1141 accctataaa ctacgtctgc cctcattatt ggagggtgaa atgtgaatac atcctattca
     1201 caatcgaatt tacgacacaa ccaaatttta atttggcttt gcattttatc tttttttagc
     1261 gtattaaatg aaatggtttt gaacgtctca ttacctgata ttgcaaatga ttttaataaa
     1321 ccacctgcga gtacaaactg ggtgaacaca gcctttatgt taaccttttc cattggaaca
     1381 gctgtatatg gaaagctatc tgatcaatta ggcatcaaaa ggttactcct atttggaatt
     1441 ataataaatt gtttcgggtc ggtaattggg tttgttggcc attctttctt ttccttactt
     1501 attatggctc gttttattca aggggctggt gcagctgcat ttccagcact cgtaatggtt
     1561 gtagttgcgc gctatattcc aaaggaaaat aggggtaaag catttggtct tattggatcg
     1621 atagtagcca tgggagaagg agtcggtcca gcgattggtg gaatgatagc ccattatatt
     1681 cattggtcct atcttctact cattcctatg ataacaatta tcactgttcc gtttcttatg
     1741 aaattattaa agaaagaagt aaggataaaa ggtcattttg atatcaaagg aattatacta
     1801 atgtctgtag gcattgtatt ttttatgttg tttacaacat catatagcat ttcttttctt
     1861 atcgttagcg tgctgtcatt cctgatattt gtaaaacata tcaggaaagt aacagatcct
     1921 tttgttgatc ccggattagg gaaaaatata ccttttatga ttggagttct ttgtggggga
     1981 attatatttg gaacagtagc agggtttgtc tctatggttc cttatatgat gaaagatgtt
     2041 caccagctaa gtactgccga aatcggaagt gtaattattt tccctggaac aatgagtgtc
     2101 attattttcg gctacattgg tgggatactt gttgatagaa gaggtccttt atacgtgtta
     2161 aacatcggag ttacatttct ttctgttagc tttttaactg cttcctttct tttagaaaca
     2221 acatcatggt tcatgacaat tataatcgta tttgttttag gtgggctttc gttcaccaaa
     2281 acagttatat caacaattgt ttcaagtagc ttgaaacagc aggaagctgg tgctggaatg
     2341 agtttgctta actttaccag ctttttatca gagggaacag gtattgcaat tgtaggtggt
     2401 ttattatcca tacccttact tgatcaaagg ttgttaccta tggaagttga tcagtcaact
     2461 tatctgtata gtaatttgtt attacttttt tcaggaatca ttgtcattag ttggctggtt
     2521 accttgaatg tatataaaca ttctcaaagg gatttctaaa tcgttaaggg atcaactttg
     2581 ggagagagtt caaaattgat ccttttttta taacaggaat tcaaatcttt ttgttccatt
     2641 aaagggcgcg attgctgaat aaaagatacg agagacctct cttgtatctt ttttattttg
     2701 agtggttttg tccgttacac tagaaaaccg aaagacaata aaaattttat tcttgctgag
     2761 tctggctttc ggtaagctag acaaaacgga caaaataaaa attggcaagg gtttaaaggt
     2821 ggagattttt tgagtgatct tctcaaaaaa tactacctgt cccttgctga tttttaaacg
     2881 agcacgagag caaaaccccc ctttgctgag gtggcagagg gcaggttttt ttgtttcttt
     2941 tttctcgtaa aaaaaagaaa ggtcttaaag gttttatggt tttggtcggc actgccgaca
     3001 gcctcgcaga gcacacactt tatgaatata aagtatagtg tgttatactt tacttggaag
     3061 tggttgccgg aaagagcgaa aatgcctcac atttgtgcca cctaaaaagg agcgatttac
     3121 atatgagtta tgcagtttgt agaatgcaaa aagtgaaatc agctggacta aaaggcatgc
     3181 aatttcataa tcaaagagag cgaaaaagta gaacgaatga tgatattgac catgagcgaa
     3241 cacgtgaaaa ttatgatttg aaaaatgata aaaatattga ttacaacgaa cgtgtcaaag
     3301 aaattattga atcacaaaaa acaggtacaa gaaaaacgag gaaagatgct gttcttgtaa
     3361 atgagttgct agtaacatct gaccgagatt tttttgagca actggatcca ggagaacaaa
     3421 aacgattttt tgaggaaagt tataaattat tttccgaacg atatggcaag caaaatattg
     3481 cttatgcaac agttcataat gatgagcaaa cccctcacat gcatttaggt gttgtgccta
     3541 tgcgtgatgg aaaactgcaa ggaaaaaatg tgtttaatcg tcaagaactg ttatggctac
     3601 aagataaatt ccccgagcat atgaaaaaac agggttttga gttgaagcgt ggtgaacgtg
     3661 gctctgaccg taaacatatt gagacagcta aatttaaaaa acaaactttg gaaaaagaga
     3721 ttgattttct agaaaaaaat ttagcagtta aaaaagatga atggactgct tatagcgata
     3781 aagttaaatc agatttagaa gtaccagcga aacgacacat gaaaagtgtt gaagtgccaa
     3841 cgggtgaaaa gtccatgttt ggtttgggaa aagaaataat gaaaacagaa aagaaaccaa
     3901 ccaaaaatgt tgttatatcg gagcgtgatt ataaaaactt agtgactgct gcgagagata
     3961 acgataggtt aaaacagcat gttagaaatc tcatgagtac tgatatggcg agagaatata
     4021 aaaaattaag taaagaacat gggcaagtta aagaaaaata tagtggtctt gtagagcgat
     4081 ttaatgaaaa tgtaaatgat tataatgagt tgcttgaaga aaacaagtct ttaaagtcta
     4141 aaataagcga tttaaagcgt gatgtgagtt taatctatga aagcactaag gaattcctta
     4201 aggaacgtac agacggctta aaagccttta aaaacgtttt taaggggttt gtagacaagg
     4261 taaaggataa aacagcacaa ttccaagaaa aacacgattt agaacctaaa aagaacgaat
     4321 ttgaactaac tcataaccga gaggtaaaaa aagaacgaag tcgagatcag ggaatgagtt
     4381 tataaaataa aaaaagcacc tgaaaaggtg tctttttttg atggttttga acttgttctt
     4441 tcttatcttg atacatatag aaacaacaag tttttatttt tacagtttac attacgctta
     4501 aagtattggt gtgattggtt ttataaggtt aatgaacctt taaaaatcct tgcacagaaa
     4561 aataccctat gttattgttt aagttaccac acaaaaacaa atttacatag ggggtttctg
     4621 tattcattat gtgcaataat aatagcatta attcagatga aaaatcaagt gtactaatcg
     4681 acaaaacaaa aagtggaaaa ataagacctt ggcgagaaaa gaaaatggca aatgtagatt
     4741 attttgaact cttgcatatt cttgaattta aaaaggctga acgtgtaaaa gattgtgcag
     4801 aggtacttga atataaagtg agttatgaaa caggagaaaa aacgttatat cgtgtgtggt
     4861 tttgtaaatc tcgattatgt ccaatgtgca actggaggag agcaatgaag catggtattc
     4921 agtcacaaaa ggttgttgca gaagttatta aacaaaaacc aacagttcgt tggcttttcc
     4981 ttacgttgac cgttagaaat gtttatgatg gtactgaatt aaataaaagt ttatcggata
     5041 tgtcaaaagg ttttaatcgt atgatgaaat ataagaaaat taataaaaac ttagttggtt
     5101 ttatgcgagc aacagaagta actgtaaata atatagataa ttcttataac caacacatgc
     5161 atgtgttggt ttgtgtagaa agcacttatt tttataacac tgaaaactat gtgaatcaaa
     5221 agcaatggat tcaattttgg aaaagagcta tgaaattaga ttatgatcca aacgttaaag
     5281 tccaaatgat tcgaccaaaa aataaacata aatctgatat aaaatcagcc attgatgaaa
     5341 ctgcaaaata tccagtaaag gatattgatt ttacaacaaa tgacgaagaa aagaatttga
     5401 agcgtttatc tgatttggag gaaggtttac accgtaaaag gttaatctct tatggtggtt
     5461 tgctaaaaga aatacataaa aaattaaacc ttgatgatgc cgaagatggg gatttgattc
     5521 atacagatga tgaagaaaaa gcagatgaag atggattttc aattattgca atgtggaact
     5581 gggaaagaca aaattatttt ataaaagagt agttcaacaa acgggccata ttgttgtata
     5641 agtgatgaaa tactgaattt aaaacttagt ttatatgtgg taaaaagttt atggaagaag
     5701 ttttaattga tgataatatg gtttttgata ttgataattt aaaaggattt cttaatgata
     5761 ccagttcatt tgggtttata gctaaagaaa ataataaaat tataggattt gcatattgct
     5821 atacactttt aagacctgat ggaaaaacaa tgttttattt acactcaata ggaatgttac
     5881 ctaactatca agacaaaggt tatggttcaa aattattatc ttttattaag gaatattcta
     5941 aagagattgg ttgttctgaa atgtttttaa taactgataa aggtaatcct agagcttgcc
     6001 atgtatatga aaaattaggt ggtaaaaatg attataaaga tgaaatagta tatgtatatg
     6061 attatgaaaa aggtgataaa taaatgaata tagttgaaaa tgaaatatgt ataagaactt
     6121 taatagatga tgattttcct ttgatgttaa aatggttaac tgatgaaaga gtattagaat
     6181 tttatggtgg tagagataaa aaatatacat tagaatcatt aaaaaaacat tatacagagc
     6241 cttgggaaga tgaagttttt agagtaatta ttgaatataa caatgttcct attggatatg
     6301 gacaaatata taaaatgtat gatgagttat atactgatta tcattatcca aaaactgatg
     6361 agatagtcta tggtatggat caatttatag gagagccaaa ttattggagt aaaggaattg
     6421 gtacaagata tattaaattg atttttgaat ttttgaaaaa agaaagaaat gctaatgcag
     6481 ttattttaga ccctcataaa aataatccaa gagcaataag ggcataccaa aaatctggt
//
