LOCUS       CP113398                2362 bp    DNA     circular ENV 06-DEC-2022
DEFINITION  MAG: Staphylococcus haemolyticus isolate 702 plasmid unnamed3,
            complete sequence.
ACCESSION   CP113398
VERSION     CP113398.1
DBLINK      BioProject: PRJNA865942
            BioSample: SAMN30148944
KEYWORDS    ENV; Metagenome Assembled Genome; MAG.
SOURCE      Staphylococcus haemolyticus (mixed culture metagenome)
  ORGANISM  Staphylococcus haemolyticus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Identification and pathogenic (toxigenic) potential of
            Staphylococcus haemolyticus and Bacillus paranthracis consortium
            isolated from bovine mastitis in Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) Microbiology Laboratory, Federal Research
            Center for Animal Husbandry named after Academy Member L.K. Ernst,
            Dubrovitsy 60, Podolsk Municipal District, Moscow Region 142132,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/30/2022 11:18:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,508
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 85
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 19 of 43
            Pseudo Genes (incomplete)         :: 24 of 43
            Pseudo Genes (internal stop)      :: 12 of 43
            Pseudo Genes (multiple problems)  :: 10 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from staphylococcus haemolyticus.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Staphylococcus haemolyticus"
                     /mol_type="genomic DNA"
                     /isolate="702"
                     /isolation_source="milk"
                     /host="bovine"
                     /db_xref="taxon:1283"
                     /environmental_sample=""
                     /plasmid="unnamed3"
                     /geo_loc_name="Russia: Saratov region"
                     /collection_date="2020"
                     /metagenome_source="mixed culture metagenome"
                     /note="metagenomic"
     gene            join(2341..2362,1..713)
                     /gene="erm(C)"
                     /locus_tag="NRZ54_12960"
     ARG             4..710
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="96.72"
     gene            1161..1637
                     /locus_tag="NRZ54_12965"
     CDS             1161..1637
                     /locus_tag="NRZ54_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WAI24196.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     ARG             4..713
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.58"
                     /coverage="96.72"
     Typing          1162..1647
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acagtcaaaa ctttattact tcaaaacata atatagataa aataatgaca aatataagat
       61 taaatgaaca tgataatatc tttgaaatcg gctcaggaaa agggcatttt acccttgaat
      121 tagtacagag gtgtaatttc gtaactgcca ttgaaataga ccataaatta tgcaaaacta
      181 cagaaaataa acttgttgat cacgataatt tccaagtttt aaacaaggat atattgcagt
      241 ttaaatttcc taaaaaccaa tcctataaaa tatttggtaa tataccttat aacataagta
      301 cggatataat acgcaaaatt gtttttgata gtatagctga tgagatttat ttaatcgtgg
      361 aatacgggtt tgctaaaaga ttattaaata caaaacgctc attggcatta tttttaatgg
      421 cagaagttga tatttctata ttaagtatgg ttccaagaga atattttcat cctaaaccta
      481 aagtgaatag ctcacttatc agattaaata gaaaaaaatc aagaatatca cacaaagata
      541 aacagaagta taattatttc gttatgaaat gggttaacaa agaatacaag aaaatattta
      601 caaaaaatca atttaacaat tccttaaaac atgcaggaat tgacgattta aacaatatta
      661 gctttgaaca attcttatct cttttcaata gctataaatt atttaataag taagttaagg
      721 gatgcagttt atgcatccct taacttgttt ttcgtgtacc tattttttgt gaatcgatta
      781 tgtcttttgc gcattcactt cttttctata taaatatgag cgaagcgaat aagcgtcgga
      841 aaagcagcaa aaagtttcct ttttgctgtt ggagcatggg ggttcagggg gtgcagtatc
      901 tgacgtcaat gccgagcgaa agcgagccga agggtagcat ttacgttaga taaccccctg
      961 atatgctccg acgctttata tagaaaagaa gattcaacta ggtaaaatct taatataggt
     1021 tgagatgata aggtttataa ggaatttgtt tgttctaatt tttcactcat tttgttctaa
     1081 tttcttttaa caaatgttct ttttttttta gaacagttat gatatagtta gaatagttta
     1141 aaataaggag tgagaaaaag atgaaagaaa gatatggaac agtctataaa ggctctcaga
     1201 ggctcataga cgaagaaagt ggagaagtca tagaggtaga caagttatac cgtaaacaaa
     1261 cgtctggtaa cttcgtaaag gcatatatag tgcaattaat aagtatgtta gatatgattg
     1321 gcggaaaaaa acttaaaatc gttaactata tcctagataa tgtccactta agtaacaata
     1381 caatgatagc tacaacaaga gaaatagcaa aagctacagg aacaagtcta caaacagtaa
     1441 taacaacact taaaatctta gaagaaggaa atattataaa aagaaaaact ggagtattaa
     1501 tgttaaaccc tgaactacta atgagaggcg acgaccaaaa acaaaaatac ctcttactcg
     1561 aatttgggaa ctttgagcaa gaggcaaatg aaaaacaaga aaatgcatta tctgattatt
     1621 attctttcaa ggactagtat aacataaaat cgtctacaaa tagacaaaaa acctgcacgc
     1681 ttaatgtaga tcaaaagctt aacgcaaatg aaatagattg acctcccaat aacaccacgt
     1741 agttattggg aggtcaatct atgaaatgcg attaagcttt ttctaattca cataagcgtg
     1801 caggtttaaa gtacataaaa aatataatga aaaaaagcat cattatacta acgttatacc
     1861 aacattatac tctcattata ctaattgctt attccaattt cctattggtt ggaaccaaca
     1921 ggcgttagtg tgttgttgag ttggtacttt catgggatta atcccatgaa acccccaacc
     1981 aactcgccaa agctttggct aacacacacg ccattccaac caatagtttt ctcggcataa
     2041 agccatgctc tgacgcttaa atgcactaat gccttaaaaa aacattaaag tctaacacac
     2101 tagacttatt tacttcgtaa ttaagtcgtt aaaccgtgtg ctctacgacc aaaagtataa
     2161 aacctttaag aactttcttt tttcttgtaa aaaaagaaac tagataaatc tctcatatct
     2221 tttattcaat aatcgcatca gattgcagta taaatttaac gatcactcat catgttcata
     2281 tttatcagag ctcgtgctat aattatacta attttataag gaggaaaaaa gagggttata
     2341 atgaacgaga aaaatataaa ac
//
