LOCUS       CP113397                3048 bp    DNA     circular ENV 06-DEC-2022
DEFINITION  MAG: Staphylococcus haemolyticus isolate 702 plasmid unnamed2,
            complete sequence.
ACCESSION   CP113397
VERSION     CP113397.1
DBLINK      BioProject: PRJNA865942
            BioSample: SAMN30148944
KEYWORDS    ENV; Metagenome Assembled Genome; MAG.
SOURCE      Staphylococcus haemolyticus (mixed culture metagenome)
  ORGANISM  Staphylococcus haemolyticus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Identification and pathogenic (toxigenic) potential of
            Staphylococcus haemolyticus and Bacillus paranthracis consortium
            isolated from bovine mastitis in Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) Microbiology Laboratory, Federal Research
            Center for Animal Husbandry named after Academy Member L.K. Ernst,
            Dubrovitsy 60, Podolsk Municipal District, Moscow Region 142132,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/30/2022 11:18:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,508
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 85
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 19 of 43
            Pseudo Genes (incomplete)         :: 24 of 43
            Pseudo Genes (internal stop)      :: 12 of 43
            Pseudo Genes (multiple problems)  :: 10 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from staphylococcus haemolyticus.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Staphylococcus haemolyticus"
                     /mol_type="genomic DNA"
                     /isolate="702"
                     /isolation_source="milk"
                     /host="bovine"
                     /db_xref="taxon:1283"
                     /environmental_sample=""
                     /plasmid="unnamed2"
                     /geo_loc_name="Russia: Saratov region"
                     /collection_date="2020"
                     /metagenome_source="mixed culture metagenome"
                     /note="metagenomic"
     gene            join(2272..3048,1..45)
                     /locus_tag="NRZ54_12950"
     CDS             join(2272..3048,1..45)
                     /locus_tag="NRZ54_12950"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="WAI24193.1"
                     /translation="MFFKCSICDEKLKRGYKTLACENNHTFDIAKEGYSNLISGHKKSK
                     YDKKLFIARRELNDSSNLYSILISKIIEIIKKYEIKTKNNVIDVGTGEGYILNEISKTF
                     LGENEFLGIDIAKEGVKKAAKSYKNINWLVADLADIPVMNGNVDFLINVLSPSNYDEFK
                     RVLDKEGKLIKIIPNSDYLKEIRNIVFSNHKNKDYDNSEVIDLFEANFNKIEMIDISYK
                     VKLNKEELNKLLDMTPLTWNLSDVEKEGIIKKNLSEITMSFKVLVGGFKTS"
                     /name="methyltransferase domain-containing protein"
     gene            228..1232
                     /locus_tag="NRZ54_12955"
     CDS             228..1232
                     /locus_tag="NRZ54_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020368559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WAI24194.1"
                     /translation="MQYNTTKCIDENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNVLEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EEVVKEKPTARWLFLTLSTKNAIDGDHLEQSLKHMSKAFNKLKMYTKVKKNLVGFLRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQVEWVDLWQKALQVNYRPVANIKAI
                     KPNQKGDKDIQAAIKETSKYSVKSSDFLTDDDEKNQEIVNDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDAEDGNLINTSDEDKTTDEEEKAHSITAIWNFEKQNYYLKDLKR"
                     /name="protein rep"
     Typing          229..1232
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.806"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acgatgagtt ttaaggtttt ggttggtggt tttaaaacga gttgaaaacg agtttcttct
       61 tgtcttgata ctatatagaa ataactcaaa gatataaaaa gttgctttat cgttggtatg
      121 acagtgtttt tatgtatttt tgtgcgtgaa agaaaaaatt gacataaaaa agctccagta
      181 atatgattaa gttacgacgc ttaaatcaat actggaggtc tttttatatg caatataata
      241 ctactaaatg tatagacgaa aatcaagata ataaaacatt aaaagatatg acgaaaagtg
      301 ggaaacaacg cccatggaga gaaaagaaga tagataatgt aagttatgca gatatactgg
      361 aaatcttaaa aattaaaaag gcttttaacg taaaacaatg tggtaacgtc ttagaattca
      421 agccaactga tgaaggttat ttgaaattac ataagacatg gttttgtaag tcaaaattat
      481 gcccagtttg taattggagg cgtgctatga aaaatagtta tcaagctcaa aaagtgattg
      541 aagaagtggt taaagaaaag ccaactgctc gttggttatt tcttacttta tcgactaaaa
      601 atgcaataga cggtgaccac ttagaacaaa gtttaaaaca catgtctaag gcttttaata
      661 aattaaagat gtatacaaaa gttaaaaaga atttggtggg ttttttacgc tcaactgaag
      721 taacagttaa taaaaatgac ggtagttata atcaacatat gcacgtttta ttgtgtgttg
      781 aaaatgcgta tttcagaaaa aaagaaaact acattacgca agttgaatgg gttgatttat
      841 ggcaaaaagc attacaggtt aattatcgac cagtagcaaa tattaaagcg atcaaaccaa
      901 atcaaaaagg cgataaagat attcaagcag ctatcaaaga aacctctaaa tattcagtta
      961 agtcatctga ttttttaact gatgatgacg aaaaaaatca agaaatcgtg aatgatttgg
     1021 aaaaaggttt ataccgaaaa cgtatgttga gttatggtgg attgcttaaa caaaaacata
     1081 aaattttaaa tttagacgat gccgaagatg gcaatttgat taatacaagt gacgaagata
     1141 aaacaacaga cgaagaagaa aaagcacatt caattacggc aatttggaat tttgaaaaac
     1201 aaaattatta cttaaaagat ttgaaacgtt agcttaaaag ctagcgtttt ttttatgtct
     1261 ttttgcccct gcgggaacta tagcgttcaa ccggcatggt tgcccaattt tcatgttaat
     1321 ttttaaattt gcatgtaact gggcagtgtt tattttttct tgcccatttt gctaactttt
     1381 aagtttgcat ataaatgggc agtgtctaaa aaattcccca ctggtcatga acaaaaaaat
     1441 gagctaaaca atttaacttt ttaagttata taaattttag ggagttccaa tcaaattaga
     1501 aaaatatata aaagagctat atcgtttttc tggcttatgt ttaaaaagta caagtgattg
     1561 tatttaaaat gactttagca atttaagtaa tatgtctttt gaatacaagt taaatgtcag
     1621 aaagatgtac ccttattttg ctcatatttg cgttttaaga aatgaatatg attatacgta
     1681 tataaaacta tataaattta gagtttgctc taaatacccc cttaaaattc aaaataaagg
     1741 catttaaaaa ttaagaaaat aaaaaagcta ctagcaaaaa actagtagct taaatcttag
     1801 ttaaatttct atgtagtatt aatttttata tctacgctac aacgtcgatt ctagttcgtg
     1861 cagtattaat ttttatatct gcgccacaac gccgattcct taaaactaag ataaaaattg
     1921 acgaaggtcg attatggttt tttacgcgtt ctttttagaa cgcataagtg cgcccttacg
     1981 ggatttaact agattataac gacgaaaaat agacctgtaa agcaataaca gttgggtcta
     2041 ctttgtctac tgggcagaca agaaaagaac acgcatttat gccgagaaaa tttattgatg
     2101 ttgagaagaa cccttaacta aacttgcaga cgaatgtcgg catagcgtga gctattaagc
     2161 cgaccattcg acaagttttg ggattgttaa gggttccgag gctcaacgtc aataaagcaa
     2221 ttggaataaa gcaactttgt tatttataat tatttgattg gaggtgtgaa tatgtttttt
     2281 aagtgttcta tatgtgatga aaaattaaag agaggttata aaactttagc atgtgagaat
     2341 aaccatactt ttgatattgc aaaagagggt tattcgaatt taatttcagg gcataaaaaa
     2401 agtaaatacg ataaaaaatt gtttattgct agaagagaat tgaatgatag tagcaatttg
     2461 tattcgatat taatttcaaa aatcatagaa attattaaaa aatatgagat taaaactaaa
     2521 aataatgtga tagatgttgg tactggtgag ggttatattt taaatgaaat tagtaaaaca
     2581 tttcttggtg aaaatgaatt cttaggtata gacattgcaa aagaaggtgt aaaaaaagca
     2641 gcaaaatcat ataaaaatat aaattggttg gttgctgatc tagctgatat accagttatg
     2701 aatgggaatg tagacttttt aattaatgtg cttagccctt ctaattatga tgagtttaaa
     2761 agagttctag ataaagaggg gaaattgata aaaataattc ctaatagtga ttatttaaaa
     2821 gaaataagga atatagtctt tagtaatcat aaaaataagg attatgataa ttctgaggtt
     2881 atagatttat ttgaagctaa ttttaataaa attgaaatga tagacattag ttataaggtt
     2941 aaattaaata aagaagaatt gaataaatta cttgatatga cgcctttaac gtggaattta
     3001 agtgacgtag aaaaagaagg aataattaag aaaaacttaa gtgaaatt
//
