LOCUS       CP113396                6539 bp    DNA     circular ENV 06-DEC-2022
DEFINITION  MAG: Staphylococcus haemolyticus isolate 702 plasmid unnamed1,
            complete sequence.
ACCESSION   CP113396
VERSION     CP113396.1
DBLINK      BioProject: PRJNA865942
            BioSample: SAMN30148944
KEYWORDS    ENV; Metagenome Assembled Genome; MAG.
SOURCE      Staphylococcus haemolyticus (mixed culture metagenome)
  ORGANISM  Staphylococcus haemolyticus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Identification and pathogenic (toxigenic) potential of
            Staphylococcus haemolyticus and Bacillus paranthracis consortium
            isolated from bovine mastitis in Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) Microbiology Laboratory, Federal Research
            Center for Animal Husbandry named after Academy Member L.K. Ernst,
            Dubrovitsy 60, Podolsk Municipal District, Moscow Region 142132,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/30/2022 11:18:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,593
            CDSs (total)                      :: 2,508
            Genes (coding)                    :: 2,465
            CDSs (with protein)               :: 2,465
            Genes (RNA)                       :: 85
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 19 of 43
            Pseudo Genes (incomplete)         :: 24 of 43
            Pseudo Genes (internal stop)      :: 12 of 43
            Pseudo Genes (multiple problems)  :: 10 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from staphylococcus haemolyticus.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Staphylococcus haemolyticus"
                     /mol_type="genomic DNA"
                     /isolate="702"
                     /isolation_source="milk"
                     /host="bovine"
                     /db_xref="taxon:1283"
                     /environmental_sample=""
                     /plasmid="unnamed1"
                     /geo_loc_name="Russia: Saratov region"
                     /collection_date="2020"
                     /metagenome_source="mixed culture metagenome"
                     /note="metagenomic"
     gene            join(5388..6539,1..111)
                     /locus_tag="NRZ54_12925"
     CDS             join(5388..6539,1..111)
                     /locus_tag="NRZ54_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WAI24189.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYD
                     LKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFE
                     ESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKF
                     PEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVK
                     SDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDND
                     RLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSK
                     ISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNE
                     FELTHNREVKKERSRDQGMSL"
                     /name="plasmid recombination protein"
     gene            355..1338
                     /locus_tag="NRZ54_12930"
     CDS             355..1338
                     /locus_tag="NRZ54_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WAI24190.1"
                     /translation="MCNNNSINSDEKSSVLIDKTKSGKIRPWREKKMANVDYFELLHIL
                     EFKKAERVKDCAEVLEYKVSYETGEKTLYRVWFCKSRLCPMCNWRRAMKHGIQSQKVVA
                     EVIKQKPTVRWLFLTLTVRNVYDGTELNKSLSDMSKGFNRMMKYKKINKNLVGFMRATE
                     VTVNNIDNSYNQHMHVLVCVESTYFYNTENYVNQKQWIQFWKRAMKLDYDPNVKVQMIR
                     PKNKHKSDIKSAIDETAKYPVKDIDFTTNDEEKNLKRLSDLEEGLHRKRLISYGGLLKE
                     IHKKLNLDDAEDGDLIHTDDEEKADEDGFSIIAMWNWERQNYFIKE"
                     /name="protein rep"
     gene            1417..1809
                     /locus_tag="NRZ54_12935"
     CDS             1417..1809
                     /locus_tag="NRZ54_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WAI24191.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYT
                     LLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACH
                     VYEKLGGKNDYKDEIVYVYDYEKGDK"
                     /name="GNAT family N-acetyltransferase"
     gene            1810..3249
                     /gene="aph(2'')-Ia"
                     /locus_tag="NRZ54_12940"
     CDS             1810..3249
                     /gene="aph(2'')-Ia"
                     /locus_tag="NRZ54_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="WAI24192.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLES
                     LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFIGE
                     PNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLPEHE
                     LHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNE
                     YIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTF
                     LTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIY
                     NDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGI
                     IDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIK
                     NIKQEFIENGRKEIYKRTYKD"
                     /name="aph(2'')-Ia"
     gene            3448..4824
                     /gene="tet(L)"
                     /locus_tag="NRZ54_12945"
     ARG             3449..4821
                     /name="tet(L)"
                     /gene="tet(L)"
                     /gene_name="tetracycline efflux MFS transporter Tet(L)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1811..3246
                     /name="aac(6')-Ie/aph(2'')-Ia"
                     /gene="aac(6')-Ie/aph(2'')-Ia"
                     /gene_name="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1811..3249
                     /name="AAC(6')-Ie-APH(2'')-Ia bifunctional protein"
                     /gene="AAC(6')-Ie-APH(2'')-Ia bifunctional protein"
                     /gene_name="aminoglycoside bifunctional resistance protein"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          356..1337
                     /name="rep_cluster_2104"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.727"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5389..6491
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.924"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 gcacaattcc aagaaaaaca cgatttagaa cctaaaaaga acgaatttga actaactcat
       61 aaccgagagg taaaaaaaga acgaagtcga gatcagggaa tgagtttata aaataaaaaa
      121 agcacctgaa aaggtgtctt tttttgatgg ttttgaactt gttctttctt atcttgatac
      181 atatagaaac aacaagtttt tatttttaca gtttacatta cgcttaaagt attggtgtga
      241 ttggttttat aaggttaatg aacctttaaa aatccttgca cagaaaaata ccctatgtta
      301 ttgtttaagt taccacacaa aaacaaattt acataggggg tttctgtatt cattatgtgc
      361 aataataata gcattaattc agatgaaaaa tcaagtgtac taatcgacaa aacaaaaagt
      421 ggaaaaataa gaccttggcg agaaaagaaa atggcaaatg tagattattt tgaactcttg
      481 catattcttg aatttaaaaa ggctgaacgt gtaaaagatt gtgcagaggt acttgaatat
      541 aaagtgagtt atgaaacagg agaaaaaacg ttatatcgtg tgtggttttg taaatctcga
      601 ttatgtccaa tgtgcaactg gaggagagca atgaagcatg gtattcagtc acaaaaggtt
      661 gttgcagaag ttattaaaca aaaaccaaca gttcgttggc ttttccttac gttgaccgtt
      721 agaaatgttt atgatggtac tgaattaaat aaaagtttat cggatatgtc aaaaggtttt
      781 aatcgtatga tgaaatataa gaaaattaat aaaaacttag ttggttttat gcgagcaaca
      841 gaagtaactg taaataatat agataattct tataaccaac acatgcatgt gttggtttgt
      901 gtagaaagca cttattttta taacactgaa aactatgtga atcaaaagca atggattcaa
      961 ttttggaaaa gagctatgaa attagattat gatccaaacg ttaaagtcca aatgattcga
     1021 ccaaaaaata aacataaatc tgatataaaa tcagccattg atgaaactgc aaaatatcca
     1081 gtaaaggata ttgattttac aacaaatgac gaagaaaaga atttgaagcg tttatctgat
     1141 ttggaggaag gtttacaccg taaaaggtta atctcttatg gtggtttgct aaaagaaata
     1201 cataaaaaat taaaccttga tgatgccgaa gatggggatt tgattcatac agatgatgaa
     1261 gaaaaagcag atgaagatgg attttcaatt attgcaatgt ggaactggga aagacaaaat
     1321 tattttataa aagagtagtt caacaaacgg gccatattgt tgtataagtg atgaaatact
     1381 gaatttaaaa cttagtttat atgtggtaaa aagtttatgg aagaagtttt aattgatgat
     1441 aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag ttcatttggg
     1501 tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac acttttaaga
     1561 cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa ctatcaagac
     1621 aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga gattggttgt
     1681 tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt atatgaaaaa
     1741 ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta tgaaaaaggt
     1801 gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat agatgatgat
     1861 tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta tggtggtaga
     1921 gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg ggaagatgaa
     1981 gtttttagag taattattga atataacaat gttcctattg gatatggaca aatatataaa
     2041 atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat agtctatggt
     2101 atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac aagatatatt
     2161 aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat tttagaccct
     2221 cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag aattattgaa
     2281 gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt aatggaatat
     2341 agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga gcattacttt
     2401 gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag tgtggcatat
     2461 ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa aaaaggttat
     2521 gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa tgtaaaaatt
     2581 cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta taaagaaatt
     2641 aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga acaaaatttg
     2701 ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta tacagatatt
     2761 agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt gttgcgtgaa
     2821 actatttata atgatttaac tgatatagaa aaagattata tagaaagttt tatggaaaga
     2881 ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga ttttagttgt
     2941 aatcatctat tgttagatgg caataataga ttaactggaa taattgattt tggagattct
     3001 ggaattatag atgaatattg tgattttata tacttacttg aagatagtga agaagaaata
     3061 ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat tgagaaagca
     3121 aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt ttatggaatt
     3181 aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa aaggacttat
     3241 aaagattgat tatataatat atgaaaagct attataaaag acattagtat taaatagttt
     3301 aaaaaaatga aaaataataa aggttttaat caagtttagg aggaattaat tatgaagtgt
     3361 aatgaatgta acagggttca attaaaagag ggaagcgtat cattaaccct ataaactacg
     3421 tctgccctca ttattggagg gtgaaatgtg aatacatcct attcacaatc gaatttacga
     3481 cacaaccaaa ttttaatttg gctttgcatt ttatcttttt ttagcgtatt aaatgaaatg
     3541 gttttgaacg tctcattacc tgatattgca aatgatttta ataaaccacc tgcgagtaca
     3601 aactgggtga acacagcctt tatgttaacc ttttccattg gaacagctgt atatggaaag
     3661 ctatctgatc aattaggcat caaaaggtta ctcctatttg gaattataat aaattgtttc
     3721 gggtcggtaa ttgggtttgt tggccattct ttcttttcct tacttattat ggctcgtttt
     3781 attcaagggg ctggtgcagc tgcatttcca gcactcgtaa tggttgtagt tgcgcgctat
     3841 attccaaagg aaaatagggg taaagcattt ggtcttattg gatcgatagt agccatggga
     3901 gaaggagtcg gtccagcgat tggtggaatg atagcccatt atattcattg gtcctatctt
     3961 ctactcattc ctatgataac aattatcact gttccgtttc ttatgaaatt attaaagaaa
     4021 gaagtaagga taaaaggtca ttttgatatc aaaggaatta tactaatgtc tgtaggcatt
     4081 gtatttttta tgttgtttac aacatcatat agcatttctt ttcttatcgt tagcgtgctg
     4141 tcattcctga tatttgtaaa acatatcagg aaagtaacag atccttttgt tgatcccgga
     4201 ttagggaaaa atataccttt tatgattgga gttctttgtg ggggaattat atttggaaca
     4261 gtagcagggt ttgtctctat ggttccttat atgatgaaag atgttcacca gctaagtact
     4321 gccgaaatcg gaagtgtaat tattttccct ggaacaatga gtgtcattat tttcggctac
     4381 attggtggga tacttgttga tagaagaggt cctttatacg tgttaaacat cggagttaca
     4441 tttctttctg ttagcttttt aactgcttcc tttcttttag aaacaacatc atggttcatg
     4501 acaattataa tcgtatttgt tttaggtggg ctttcgttca ccaaaacagt tatatcaaca
     4561 attgtttcaa gtagcttgaa acagcaggaa gctggtgctg gaatgagttt gcttaacttt
     4621 accagctttt tatcagaggg aacaggtatt gcaattgtag gtggtttatt atccataccc
     4681 ttacttgatc aaaggttgtt acctatggaa gttgatcagt caacttatct gtatagtaat
     4741 ttgttattac ttttttcagg aatcattgtc attagttggc tggttacctt gaatgtatat
     4801 aaacattctc aaagggattt ctaaatcgtt aagggatcaa ctttgggaga gagttcaaaa
     4861 ttgatccttt ttttataaca ggaattcaaa tctttttgtt ccattaaagg gcgcgattgc
     4921 tgaataaaag atacgagaga cctctcttgt atctttttta ttttgagtgg ttttgtccgt
     4981 tacactagaa aaccgaaaga caataaaaat tttattcttg ctgagtctgg ctttcggtaa
     5041 gctagacaaa acggacaaaa taaaaattgg caagggttta aaggtggaga ttttttgagt
     5101 gatcttctca aaaaatacta cctgtccctt gctgattttt aaacgagcac gagagcaaaa
     5161 cccccctttg ctgaggtggc agagggcagg tttttttgtt tcttttttct cgtaaaaaaa
     5221 agaaaggtct taaaggtttt atggttttgg tcggcactgc cgacagcctc gcagagcaca
     5281 cactttatga atataaagta tagtgtgtta tactttactt ggaagtggtt gccggaaaga
     5341 gcgaaaatgc ctcacatttg tgccacctaa aaaggagcga tttacatatg agttatgcag
     5401 tttgtagaat gcaaaaagtg aaatcagctg gactaaaagg catgcaattt cataatcaaa
     5461 gagagcgaaa aagtagaacg aatgatgata ttgaccatga gcgaacacgt gaaaattatg
     5521 atttgaaaaa tgataaaaat attgattaca acgaacgtgt caaagaaatt attgaatcac
     5581 aaaaaacagg tacaagaaaa acgaggaaag atgctgttct tgtaaatgag ttgctagtaa
     5641 catctgaccg agattttttt gagcaactgg atccaggaga acaaaaacga ttttttgagg
     5701 aaagttataa attattttcc gaacgatatg gcaagcaaaa tattgcttat gcaacagttc
     5761 ataatgatga gcaaacccct cacatgcatt taggtgttgt gcctatgcgt gatggaaaac
     5821 tgcaaggaaa aaatgtgttt aatcgtcaag aactgttatg gctacaagat aaattccccg
     5881 agcatatgaa aaaacagggt tttgagttga agcgtggtga acgtggctct gaccgtaaac
     5941 atattgagac agctaaattt aaaaaacaaa ctttggaaaa agagattgat tttctagaaa
     6001 aaaatttagc agttaaaaaa gatgaatgga ctgcttatag cgataaagtt aaatcagatt
     6061 tagaagtacc agcgaaacga cacatgaaaa gtgttgaagt gccaacgggt gaaaagtcca
     6121 tgtttggttt gggaaaagaa ataatgaaaa cagaaaagaa accaaccaaa aatgttgtta
     6181 tatcggagcg tgattataaa aacttagtga ctgctgcgag agataacgat aggttaaaac
     6241 agcatgttag aaatctcatg agtactgata tggcgagaga atataaaaaa ttaagtaaag
     6301 aacatgggca agttaaagaa aaatatagtg gtcttgtaga gcgatttaat gaaaatgtaa
     6361 atgattataa tgagttgctt gaagaaaaca agtctttaaa gtctaaaata agcgatttaa
     6421 agcgtgatgt gagtttaatc tatgaaagca ctaaggaatt ccttaaggaa cgtacagacg
     6481 gcttaaaagc ctttaaaaac gtttttaagg ggtttgtaga caaggtaaag gataaaaca
//
