LOCUS       CP113394                3124 bp    DNA     circular ENV 13-DEC-2022
DEFINITION  MAG: Bacillus paranthracis isolate 1710 plasmid unnamed9, complete
            sequence.
ACCESSION   CP113394
VERSION     CP113394.1
DBLINK      BioProject: PRJNA865942
            BioSample: SAMN30148864
KEYWORDS    ENV; Metagenome Assembled Genome; MAG.
SOURCE      Bacillus paranthracis (mixed culture metagenome)
  ORGANISM  Bacillus paranthracis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Identification and pathogenic (toxigenic) potential of
            Staphylococcus haemolyticus and Bacillus paranthracis consortium
            isolated from bovine mastitis in Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) Microbiology Laboratory, Federal Research
            Center for Animal Husbandry named after Academy Member L.K. Ernst,
            Dubrovitsy 60, Podolsk Municipal District, Moscow Region 142132,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/29/2022 13:02:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,182
            CDSs (total)                      :: 6,034
            Genes (coding)                    :: 5,809
            CDSs (with protein)               :: 5,809
            Genes (RNA)                       :: 148
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 97 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 63 of 225
            Pseudo Genes (multiple problems)  :: 39 of 225
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Bacillus paranthracis.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Bacillus paranthracis"
                     /mol_type="genomic DNA"
                     /isolate="1710"
                     /isolation_source="milk"
                     /host="bovine"
                     /db_xref="taxon:2026186"
                     /environmental_sample=""
                     /plasmid="unnamed9"
                     /geo_loc_name="Russia: Mordovia region"
                     /collection_date="2020"
                     /metagenome_source="mixed culture metagenome"
                     /note="metagenomic"
     gene            581..949
                     /locus_tag="NRZ52_30900"
     CDS             581..949
                     /locus_tag="NRZ52_30900"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI35837.1"
                     /translation="MKKMVQVLLVSFLVLTSVAFVKPSSSYALDCLGRDRPTCAKWDYL
                     RILQKIPKSLKKSNEVVDLKKFSQKIRGTNDYKDPKSGWIISKNKGKSHGGSAWKLYNK
                     GKKERIASLTSSGKVLRE"
                     /name="hypothetical protein"
     gene            974..1177
                     /locus_tag="NRZ52_30905"
     CDS             974..1177
                     /locus_tag="NRZ52_30905"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI35838.1"
                     /translation="MAKPLFCPNCGVKLIDEDFYVKSFTSYETKDRNNNVVVIDLNGWG
                     CECASCNWEGDILPYDEDTEYN"
                     /name="hypothetical protein"
     gene            1495..2448
                     /locus_tag="NRZ52_30910"
     CDS             1495..2448
                     /locus_tag="NRZ52_30910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WAI35839.1"
                     /translation="MTKKNQDEIVWKYQPKKKQNLKIYEFIKDKLSEGGRELFKECSTF
                     NQFISTRDKIKNKLIGSNPCKNRFCPICAWRKARKDAMKIATMMEAIKVEEKKEFLFLT
                     LTTPNIKADMVKSEIDRFNKAFKKLFDRKKVQRSIKGYIRKLEMTYNKERDDYNPHFHV
                     LLCVDKNYFKRKELYIKQQEWLSMWREATDLPEITQVHIQKVELIREGNAVGEIAKYSA
                     KDYELATNQEVFDVFYASLKGRQLITFNGLFKEYALKYENGELDKYKTKDKNEYFYKIL
                     ATWNPDLLKFNREYTELTEEEKEKYNGHLIDEMEVE"
                     /name="protein rep"
     Typing          1496..2402
                     /name="rep_cluster_1151"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.742"
                     /coverage="95.0"
                     /evalue="0.0"
ORIGIN
        1 ttttatatca ttctatgatt tctttatggt ttttttagtt agagcaaaaa aataataagt
       61 tagtagatcg taaggagatt tttaaaattt gacgagacat aggggaattt aacgaggcgt
      121 ggcgaggaaa aaatgcaggg cagcggggat gtattctcgg attttagcga ggcataggga
      181 ggcgtggtga atgtaaacga agactctttg atactttggt tttttctcgt gaacggtgga
      241 ggtggttatc tggcgtttgt ccaacggtaa gcggaacgta gtgagcatta aggagtggac
      301 gaacttgcta cactgaagac cctcttgttg tctcccaaac tttacgtttg ggggctggct
      361 accgccgagg gagtccttcc gagatcggaa gggttgggta ggttgagttt ttgtcttacg
      421 aatcatctaa aaaaaagata atgaaattaa gatttaataa agttaaatat tcccttttat
      481 atatgtttaa ttttactata tatagaattc aaaaaccata cttgtattat atttccatta
      541 atcttagaat ttaggaaaaa ccaatgcgag gtgttttgag atgaaaaaaa tggtacaagt
      601 tttattagtt agttttcttg ttttaacttc agttgcattt gttaaacctt cttcttcgta
      661 tgctttagat tgtttgggaa gagataggcc gacttgtgca aaatgggatt atttaagaat
      721 acttcaaaag atacctaaat ctttaaaaaa atctaatgaa gttgttgatt tgaagaagtt
      781 ttcgcaaaaa attagaggta ctaatgatta caaagatcct aagtcaggtt ggattatttc
      841 taaaaataag ggtaaatcac atggtggttc agcgtggaag ttgtataata aaggcaaaaa
      901 ggaacgtatt gcttctttaa cgagtagtgg aaaagtgtta agagaataaa aaaatattaa
      961 gggtgattgt tttatggcta aacctttgtt ttgtcctaat tgtggagtta aattaattga
     1021 tgaagatttt tatgtaaaga gttttactag ttatgaaact aaagatcgta ataacaatgt
     1081 agttgtaatt gatttaaatg gatggggatg tgaatgtgct tcctgtaatt gggaaggtga
     1141 cattttacct tacgatgaag acactgaata taattgattt actaaaaaaa agtagagagg
     1201 aaaacgaatc gttttcctct ctactttttt gcgattttcc ggaacggaaa atcgtgtttt
     1261 ttcttttctt gatacaaggg taactatttc ggcggcgaaa tagttaccct tgtatgcctt
     1321 aaagccttgt ggttgtaagg tttttaataa aaaaagactt gctttttcag gcggatagat
     1381 ttaacgtttt aattgtcgag aaaaaaacaa taaactatca cgaaaggagc aagtctcttt
     1441 ttaaagggtt ggtgtaccct tttttactat tcaactggga ctaatatatc agatatgacg
     1501 aaaaaaaatc aagacgaaat tgtttggaag tatcaaccga agaaaaaaca gaatttaaaa
     1561 atctatgaat ttattaaaga taaattgagt gagggtggac gtgaattgtt taaagagtgt
     1621 agtactttta accaatttat ttcaactcgt gacaaaataa aaaataaatt aatagggtct
     1681 aatccttgta aaaataggtt ttgtccgatt tgtgcttgga ggaaagctcg taaagatgcg
     1741 atgaaaatag ctacgatgat ggaagctatc aaggttgagg aaaagaaaga gtttttattt
     1801 ctcactttga ctacgccgaa tattaaagct gatatggtga aaagtgaaat tgatagattt
     1861 aataaagctt ttaagaaatt gtttgataga aaaaaggttc aaagaagtat taaagggtat
     1921 attagaaaat tagagatgac ttacaacaaa gaacgtgatg actataatcc gcattttcat
     1981 gttttacttt gtgtagataa aaattatttc aaacgtaaag agctttacat taaacaacaa
     2041 gaatggcttt cgatgtggcg agaagctacg gatctacctg aaataacaca agtacatatt
     2101 cagaaagtgg aattgattcg agaagggaat gcagttggtg agattgcgaa gtattcagcg
     2161 aaagattatg agctggcaac aaaccaagaa gtgtttgatg tgttttatgc atcgttgaaa
     2221 ggccgacaac tgattacgtt taatggactg tttaaagagt atgctttgaa gtatgaaaat
     2281 ggtgagttgg acaagtataa aacaaaagat aaaaacgagt atttttataa gatattagca
     2341 acatggaatc ctgatttatt gaaatttaat cgtgaatata ctgagttaac tgaagaagaa
     2401 aaagaaaaat ataatgggca tttgattgat gaaatggaag tggaatgaaa aaaagaacct
     2461 ggattagttc ctggttcttt tttatttttt ttatgttaac taaggtatga atactttaaa
     2521 tttaatcatc attcttttgt tttatattgt tgcgaaggtg aatgagtaag aactccactg
     2581 aatatgaatc tgttctttta atttgtgtta ttgcgaaggt gaacagaaaa aaacttcgct
     2641 gaatttcgtt tttattttac cacaaaatta tttttttggt gcaattttag tgaccactga
     2701 gtgaccactg agtgccactg aatgatcact agaacgaaaa aatgaccacc cagtgaccac
     2761 tggataaaaa agaaccgtcc cagttacaac attggtttca tggtcatttg gtggtcattt
     2821 tagacgcata caaaacacat attttcacat acaaaacgca tgcaaatagc cgaaaaacgc
     2881 atacagaacg catacaaccg aaaaaagtct tcccacaatt cacaaactag aaacccaata
     2941 ggcacagcct atttcaccat tttttttata aagtgggatt cggtttcctt atgctctttt
     3001 tttatttctt ggtttttaaa gcgtttaaaa tgattcagaa tcgcttttaa tgattttatg
     3061 tagttgaatt cattagaaaa aaataaagcg tttaaaacga attctgaacg ttctgagacg
     3121 tatt
//
