LOCUS       CP113392                3897 bp    DNA     circular ENV 13-DEC-2022
DEFINITION  MAG: Bacillus paranthracis isolate 1710 plasmid unnamed7, complete
            sequence.
ACCESSION   CP113392
VERSION     CP113392.1
DBLINK      BioProject: PRJNA865942
            BioSample: SAMN30148864
KEYWORDS    ENV; Metagenome Assembled Genome; MAG.
SOURCE      Bacillus paranthracis (mixed culture metagenome)
  ORGANISM  Bacillus paranthracis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Identification and pathogenic (toxigenic) potential of
            Staphylococcus haemolyticus and Bacillus paranthracis consortium
            isolated from bovine mastitis in Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) Microbiology Laboratory, Federal Research
            Center for Animal Husbandry named after Academy Member L.K. Ernst,
            Dubrovitsy 60, Podolsk Municipal District, Moscow Region 142132,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/29/2022 13:02:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,182
            CDSs (total)                      :: 6,034
            Genes (coding)                    :: 5,809
            CDSs (with protein)               :: 5,809
            Genes (RNA)                       :: 148
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 97 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 63 of 225
            Pseudo Genes (multiple problems)  :: 39 of 225
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Bacillus paranthracis.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Bacillus paranthracis"
                     /mol_type="genomic DNA"
                     /isolate="1710"
                     /isolation_source="milk"
                     /host="bovine"
                     /db_xref="taxon:2026186"
                     /environmental_sample=""
                     /plasmid="unnamed7"
                     /geo_loc_name="Russia: Mordovia region"
                     /collection_date="2020"
                     /metagenome_source="mixed culture metagenome"
                     /note="metagenomic"
     gene            929..1423
                     /locus_tag="NRZ52_30865"
     CDS             929..1423
                     /locus_tag="NRZ52_30865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WAI35831.1"
                     /translation="MEVENNTEKGDEVPKQRTENEIIYNGKSRWRNLDTGEIIETDEVI
                     KKTPRNGFMITYLTAIVQLIDSLGNKKMQVVKYILENMDKSTNTLIITTRELTEKSKVS
                     RQTVIDTLKTLEQAQIITRRTGAIMIHPSLVHRGKDTKEKYLLARFEDFSNNKAPINEV
                     E"
                     /name="replication/maintenance protein RepL"
     gene            1438..1722
                     /locus_tag="NRZ52_30870"
     CDS             1438..1722
                     /locus_tag="NRZ52_30870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WAI35832.1"
                     /translation="MKATGITRKVDELGRIVIPKELRKTLKIDIKDPIEIFTEEDSIIL
                     KKYESNMTCIITGEISSKNVSLFNGKIIVSPEGAELLAKELKKIITPVK"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            complement(1755..3071)
                     /locus_tag="NRZ52_30875"
     CDS             complement(1755..3071)
                     /locus_tag="NRZ52_30875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WAI35833.1"
                     /translation="MNKFAIIHMQKFQISDVQGIQKHNQRQGKSKSNLDIDYSKSEQNY
                     DLLNQQKIRYESTIKQEISERVKRKPRANSVVLSEFVVTASPDYMHSLSLEEQKRYFES
                     SLDFIQKRYGKQNTLYAMVHMDEATPHMHIGVMPITEDNRLSAKDIFTKKELVSLQQDF
                     PLEMREKGFDVERGEGSEKKHLSPQAFKEKQDLEVEVEHLSNVKTHLKTKVVETHNQLQ
                     QTTSYIEKQNETLQKIQQQFLSLDKKVKEKKQEFETFRNQIPDKPVSTSYLREETKTEV
                     TTKLFGKPEITEKKTGNIVVTREQWRDMTEKVNAAVIVKKDYERLQKTDLVKENKELHS
                     AVDEICDSLKESQKRNVGLQKENKQLSTEISSLKAHIRDLQTNIKVLYQQTKQVFKEQF
                     KTFRGLIKNELVGREVENHFEREHMREMKSRQKGYDMER"
                     /name="plasmid recombination protein"
     gene            complement(3568..3849)
                     /locus_tag="NRZ52_30880"
     CDS             complement(3568..3849)
                     /locus_tag="NRZ52_30880"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI35830.1"
                     /translation="MFFINKYSVILFSCLFVLISPLTAFAKAEMNIEQRLFINFENKVS
                     DLVITGMELLSNLDKMNGEISEEELYNLALNVGEEFSTRSKILNQISV"
                     /name="hypothetical protein"
     Typing          967..1423
                     /name="rep_cluster_1950"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.323"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1759..3071)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.785"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagcaataat tttattttaa aaatcaaaaa gggcttattt gggtatatac ataaatttgt
       61 accctgcata cgttgataaa gtgcaaccac ccaactggta attcccacaa aacttttgaa
      121 aggacctatc atgaacgatg caatttgcaa atctttaaag acaatccttt gtaggaaaaa
      181 acatgaacat ttaagggcta cctgtagaga tccttgcaag ttcctgccac cataccctta
      241 gaataagtca cttttttaag tggcttattt tttataatgt attgtgataa actataatcg
      301 aaataaaaaa attttcctta actaaatatt ttcaaacaca tatacatggt gtaagggagt
      361 cacactcctt attgaacatg tataaaaaag gctaaaatcc tttcaccagg attttagcct
      421 ttttgtgttt ttccttgttt ttgggtgtga gtgctggctg gggggttggg ggcctagccc
      481 ccaggaactt tacgtaacta catagggagt gaaaatgttg cttagaacga tagaactggg
      541 tggttcggct ggtacaattg ttggtccaag tcgagcaact taaccccgtt tttggtctgt
      601 tcggcttggc gaaagctaaa tatcagccga atttcgaaat gcaatggagg aaaggtggag
      661 attttttgag cgtttttttc aaaaaatact acctgaccat tgcatggaga aaaacccttt
      721 aaagaaaaaa caggatctat atatttaagg cgatttttat cgccttaaat atatagataa
      781 gagtaacaaa gaggttaaaa gtgaattttg agttgatata tagcttttga gacgtatttt
      841 gcttggtcta attttttata ccttttggtc taatttttta taccttttgg tctaattttt
      901 tttgaccatt tggtctaaag gtgataaaat ggaagtagaa aataatacag aaaaaggtga
      961 tgaagtgcca aaacaaagaa ctgaaaatga aataatttat aacggtaaaa gtcgatggag
     1021 aaacttagat acaggtgaaa taatagaaac tgatgaagtc ataaagaaaa ctcctagaaa
     1081 tggttttatg attacgtatt taacagcaat tgttcaactg atagatagtt taggtaataa
     1141 aaaaatgcaa gtagtaaaat acatattaga aaacatggac aagtcaacaa atacattaat
     1201 tataacgaca agagaattga ctgaaaaaag caaggtatct agacaaactg taatagacac
     1261 attaaaaaca ctagaacaag cccaaatcat tactagaaga actggagcaa taatgattca
     1321 cccaagcctt gttcatcgtg gtaaagacac aaaagagaaa tacttacttg ctagatttga
     1381 agattttagt aataataaag caccaattaa tgaagttgaa taaaggagag aataaaaatg
     1441 aaagctactg gtataactcg taaagttgat gaattaggac gtatcgtaat cccaaaagaa
     1501 ttacgtaaaa cactaaagat tgatatcaaa gatccaatag aaattttcac agaggaagat
     1561 agtattatac ttaaaaaata tgaatctaac atgacttgta taataactgg agaaatttct
     1621 agcaaaaatg tttctttatt taacggaaaa attattgtaa gtcctgaagg cgctgaatta
     1681 ttagcaaaag aattaaaaaa aattattact ccagtcaaat aaaaacgccc tagaatatct
     1741 ctaaggcgtt ttttctaacg ctccatgtca tatcctttct gtctactttt catttctctc
     1801 atatgttcac gttcaaaatg attttctacc tctcttccaa ccaattcatt tttaataagc
     1861 cctctaaacg tcttaaattg ctctttaaag acttgtttcg tttgttgata caaaaccttt
     1921 atgtttgtct gtaaatctct tatatgagcc tttaatgagc ttatttctgt acttaattgt
     1981 ttgttttcct tttgcaaccc tacatttctc ttttgagatt ctttaagtga gtcacatatc
     2041 tcatcgactg cactatgtaa ttctttgttt tcttttacaa gatccgtttt ttgcaaacgc
     2101 tcatagtcct tcttaactat aaccgctgca tttactttct ctgtcatatc tctccattgc
     2161 tcacgtgtta ccacgatgtt tcctgttttt ttctctgtga tttctggttt tccaaacagt
     2221 tttgttgtta cttccgtttt ggtttcttct cgtaaataag acgttgacac tggcttgtca
     2281 ggaatttggt ttcgaaacgt ttcaaattcc tgtttctttt cttttacttt tttatctaaa
     2341 ctcaaaaatt gttgctgaat tttttgtaac gtttcattct gtttttcgat gtagcttgta
     2401 gtttgttgta attgattatg agtttctact acttttgtct tcaaatgcgt ttttacattg
     2461 gataagtgtt ccacttctac ttctaaatct tgtttttcct taaaggcttg aggcgataaa
     2521 tgttttttct cagaaccttc tccccgctct acatcaaaac ctttttctcg catttccaac
     2581 ggaaagtcct gttgtaacga taccagttct tttttagtga aaatatcttt agctgataaa
     2641 cgattatctt cagtaattgg cataacacca atatgcatat gtggtgttgc ttcatccata
     2701 tgaaccatcg catacaacgt attttgtttg ccataacgct tttgaataaa atctagtgaa
     2761 ctctcaaaat aacgtttctg ttcttctaga cttaatgaat gcatgtaatc aggcgaagct
     2821 gttactacaa attcagaaag tacaacagaa tttgcccgtg gctttcgttt taccctctca
     2881 ctgatttctt gtttaattgt actttcatat ctaatttttt gttgattcaa caaatcataa
     2941 ttttgttcac ttttcgaata atcaatgtcc aaatttgatt tactttttcc ttgtctctga
     3001 ttatgttttt gaatcccttg cacatcagaa atttgaaact tctgcatgtg aatgattgca
     3061 aatttgttca tgtttttccc tcacaattgt cttccgtagg aggactactt ttgagcctat
     3121 cataacatat cgagagagcg aatccaaagt tttggtatag cccactatac caaattacat
     3181 ttggtgtggg ctctgcgaga ccttgttggc ttcgccaaat cacgccacag cgtgataaag
     3241 acaaggggct ttgccccaca ccccacaagg ggaattcttc cccttgaccc catcaatcga
     3301 actccccaac ccctcaatcc ttgaggggct tcctcgccgg tcggcgtccc caaaacggtt
     3361 cgttttgagg aactgccaag aggatggaga atcggctgca cgtgtcaatt ccttaatgct
     3421 cctttcatgg atcagcacaa accgctgaac catttcagtc cgcttaccat tgacacttac
     3481 cgattatttt tcttctcccc ataatcgttt ggccagttgt tttaaccgct cgttaaattt
     3541 acaatatcct cttttataat attaagtcta aacactaatt tggttcaaaa ttttagaacg
     3601 tgttgaaaat tcctcgccta catttaatgc taaattatat agttcttctt cacttatttc
     3661 accattcatc ttatctaaat tacttaataa ttccatgcca gtaatcacca gatcagaaac
     3721 tttgttttca aaatttataa aaagacgttg ctctatattc atttcagctt tagcaaaggc
     3781 tgttagtggg gaaatcaaaa caaataaaca actgaataaa attacactat atttatttat
     3841 aaaaaacatg tctatctcca cctgagtttt tattttatct aaccttatga tgttcta
//
