LOCUS       CP113391                5235 bp    DNA     circular ENV 13-DEC-2022
DEFINITION  MAG: Bacillus paranthracis isolate 1710 plasmid unnamed6, complete
            sequence.
ACCESSION   CP113391
VERSION     CP113391.1
DBLINK      BioProject: PRJNA865942
            BioSample: SAMN30148864
KEYWORDS    ENV; Metagenome Assembled Genome; MAG.
SOURCE      Bacillus paranthracis (mixed culture metagenome)
  ORGANISM  Bacillus paranthracis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Identification and pathogenic (toxigenic) potential of
            Staphylococcus haemolyticus and Bacillus paranthracis consortium
            isolated from bovine mastitis in Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) Microbiology Laboratory, Federal Research
            Center for Animal Husbandry named after Academy Member L.K. Ernst,
            Dubrovitsy 60, Podolsk Municipal District, Moscow Region 142132,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/29/2022 13:02:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,182
            CDSs (total)                      :: 6,034
            Genes (coding)                    :: 5,809
            CDSs (with protein)               :: 5,809
            Genes (RNA)                       :: 148
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 97 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 63 of 225
            Pseudo Genes (multiple problems)  :: 39 of 225
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Bacillus paranthracis.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Bacillus paranthracis"
                     /mol_type="genomic DNA"
                     /isolate="1710"
                     /isolation_source="milk"
                     /host="bovine"
                     /db_xref="taxon:2026186"
                     /environmental_sample=""
                     /plasmid="unnamed6"
                     /geo_loc_name="Russia: Mordovia region"
                     /collection_date="2020"
                     /metagenome_source="mixed culture metagenome"
                     /note="metagenomic"
     gene            join(4983..5235,1..320)
                     /locus_tag="NRZ52_30835"
     CDS             join(4983..5235,1..320)
                     /locus_tag="NRZ52_30835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017673839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WAI35826.1"
                     /translation="MHYNPYIYPYQYLYYVNVPIPMYNYGRQSVYRTFPNEIEHANRFD
                     SFRSSNNDRRISLTDYGPKPFVVNINEATKQNNTYRTALWTGTHLQVTLMSLNVGEDIG
                     LEVHSNVDQFLRIEQGQGIIQMGKNKDNLNFKRNVYDDYAIMIPAGTWHNLTNTGNIPL
                     KLYSIYAPPNHPFGTVHVTKADAMVNE"
                     /name="cupin domain-containing protein"
     gene            510..1250
                     /locus_tag="NRZ52_30840"
     CDS             510..1250
                     /locus_tag="NRZ52_30840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016083310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="WAI35827.1"
                     /translation="MKKVLEREEFVDIGGFQLYTKIFGSDKGTPTVVMDAGYGDYSKAW
                     NEIVSQIAEFTKVLVYDRAGLGKSDSSPNNRVSSEMVKELRTLLKKLQLTPPYIFVGHS
                     FGGVNVRLYSSLYPSEVAGLILVDSTPEDYRERFLPIMPSDFQEAYNKQFIYEGTYDQF
                     MKSLEEVRLHRKTLIDTNVIILSAGKKAFYSTDAQNLWLHMQKELLDLSTNSSLVIAPN
                     SGHYIQKDEPQYLINAVKKVLQIL"
                     /name="alpha/beta hydrolase"
     gene            1960..2523
                     /locus_tag="NRZ52_30845"
     CDS             1960..2523
                     /locus_tag="NRZ52_30845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WAI35828.1"
                     /translation="MEKHDENLEYHAETQTLVGQKKRELVDTETGEVIHVDQITKRVYG
                     TKNFWKMYLMDFLTVLGIIDNKQLDVFIYIAENTNQSNNLFIGTYRSISKDVGVGVSTV
                     TRIMKKLQESNFIKKKQNGVYIVNPNIMMKGNDTKRQILLSYYEEDKPLNSIDVLRGKQ
                     KALPEQETTKNESKKLGLETKNND"
                     /name="replication/maintenance protein RepL"
     gene            2516..2710
                     /locus_tag="NRZ52_30850"
     CDS             2516..2710
                     /locus_tag="NRZ52_30850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016132552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI35829.1"
                     /translation="MIEGVELNIYLDDDTISFSLSPVQTEVIFKALGLQFDAKTQTLSS
                     FSDNSLQKHVLPKINFVPK"
                     /name="hypothetical protein"
     gene            complement(2741..2926)
                     /locus_tag="NRZ52_30855"
     CDS             complement(2741..2926)
                     /locus_tag="NRZ52_30855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI35824.1"
                     /translation="MKKLRRNAIQCRKCGDVIESKFTHDFKYCSCGSVGVDGGLEYARI
                     IGDSENVIELHEYEDK"
                     /name="hypothetical protein"
     gene            complement(2941..4167)
                     /locus_tag="NRZ52_30860"
     CDS             complement(2941..4167)
                     /locus_tag="NRZ52_30860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WAI35825.1"
                     /translation="MSYAICRMQKFKMRDVKGIQIHNQREKESHTNHDIESERTHLNYD
                     LHNDGHIDYLKTVKDKIEQNVETNRAIRKDAVVMCEFVVTSDREFFDVLDMVDPAQQKV
                     FFKEAYSFLKDRYGERNIVHAAVHLDEKTPHMHVGMVPVTEENKLSAKQIFNRKELVSL
                     QDDFHAHMVEKGFYLERGVSSDKKHVEMRKFKAMTAKEEIKALETEIGQCLQKKEVVNK
                     QMEQLESRLNQLQNAVKSTNKVDKMEVKHKGGILRSKTVEMSVEDFEELKTKAKASEAF
                     KRENMTLHRKNEALQANNTDLHTTVKRLEKENEGLKPYKGKYERLAKLFDEMNKFYEKF
                     IPKEIPRFHEIIGFCKRKVNGSINRFSSLRYSEKALNENEKKGYESASKFLATEQKQQR
                     KERGNEREL"
                     /name="plasmid recombination protein"
     Typing          1961..2523
                     /name="rep_cluster_1909"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.823"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2996..4167)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.141"
                     /coverage="95.0"
                     /evalue="0.0"
ORIGIN
        1 ggacaggaac acatttacaa gttactttaa tgagtctcaa tgttggcgaa gatatcggtt
       61 tggaagtaca ttctaacgtt gatcaattct tacgtattga acaaggccag gggattattc
      121 aaatgggcaa gaataaagat aatttaaact ttaaaagaaa tgtctatgat gattatgcca
      181 taatgatacc cgctggaaca tggcataatc taaccaatac aggtaatatt cctctaaaac
      241 tttactcgat atatgctcct cctaaccatc catttggtac tgtacatgta acaaaagccg
      301 atgccatggt aaatgaataa aactattaaa aataataaaa aggatcttag attgaagatc
      361 ctttttttat gccaataata ggacgttatg ttaacttcac atgcatatgg tattcaaaat
      421 agatttctga ttttcctttt tcatccatga tacaatttat taaataattt acttgttgta
      481 ccttggaata tggaaggagt taaatctgaa tgaaaaaggt tttagaacgt gaggaatttg
      541 ttgatatagg tggttttcaa ttatatacaa aaatatttgg aagtgataaa ggaactccaa
      601 ccgtagtaat ggacgcaggt tatggtgatt actctaaagc ttggaatgaa atagtatcac
      661 aaattgcgga attcacaaaa gttctggtct atgatagagc tggtttagga aagagtgatt
      721 ctagtccgaa caatagagtt agttctgaaa tggtaaaaga gctaagaaca ttactaaaaa
      781 aattacaatt aacgcctcct tatatttttg tggggcattc ctttggtgga gtaaatgttc
      841 gcttatactc atctttatat ccaagtgagg tagcagggct aatacttgtg gattcaaccc
      901 cagaggacta cagagaacga tttcttccca tcatgccttc agatttccaa gaagcttata
      961 ataaacaatt tatttatgaa ggtacatacg atcagtttat gaagagtcta gaagaagtac
     1021 gattacacag aaaaacactt atagatacaa atgttattat tctttctgca ggaaaaaaag
     1081 ctttttattc cactgatgca caaaacttat ggttgcatat gcaaaaggaa ttattggatt
     1141 tatcaactaa tagttcctta gttatagcac ctaacagtgg acactatatc caaaaggatg
     1201 agccacaata tctaataaat gcggtcaaaa aggttcttca aatcttataa ttaacttccg
     1261 agttcgagaa ttatgtgaac atgaaagtcc cccagtcaaa caatctggag gactttttta
     1321 tggtttgttc ttgtcttttg gtcttacatt tgctttttgg ctagagtgct ggctcgtcgt
     1381 gtgggggcga aaccccacgt attacgtatc tattttttga ataaggacta ttttggaacg
     1441 aatgatctaa gcggttcggc tcgtatactt gcttgtccaa gtcgagaacc attgctttgc
     1501 tttttgtctg tcggcttggc gagaaaagca cacgagccga aaaaacttcc gcaaggatgg
     1561 agcaaagcgg aaaaggtgga gattttttga gtgtttttct caaaaaatac tacctgttcc
     1621 ttgtagaagt tttagctctt aaggaaaaaa agaaaaaaag accaactaaa agttggtctt
     1681 ttttaaaata tcaaaggggc taaatagccc ctttgatatt agaagagagg aaacgtgcat
     1741 aaaaagtggg ataaacccag ttatatcaac ggatttcacg tttttgcttg tgttcatatt
     1801 tgatagcaat tgtgttcatt tttgaagatg tctgtattct ttttgtgaac acaattgtat
     1861 tttttatata agtatgttag tgttcttata aagagttatt tggtgttcag gtatgaatac
     1921 acattgaata tatattaaat acatattggg ggatatttca tggaaaaaca tgacgagaat
     1981 ttagaatatc atgcagaaac tcaaacatta gttggtcaaa agaaacgaga attagtagac
     2041 actgaaactg gagaagttat tcatgtagac caaataacca aaagagtata tggaactaag
     2101 aatttttgga agatgtactt aatggatttc ttaacagttc taggaatcat agacaacaaa
     2161 caactagatg tttttatata catagcggaa aatacaaacc aatctaacaa tctttttata
     2221 ggtacatata gaagtatctc aaaagatgtt ggtgtcggtg taagtacagt gacacgtatt
     2281 atgaaaaaac tacaagaaag taattttatc aagaaaaaac aaaatggcgt ttatatcgta
     2341 aatcctaaca tcatgatgaa agggaacgat acaaaacgac aaatattatt aagttattac
     2401 gaagaagata agcctttaaa ttctatcgac gtattaagag ggaaacaaaa ggctcttcca
     2461 gaacaagaaa caacaaaaaa cgaatcaaaa aaattaggat tggagacgaa aaacaatgat
     2521 tgagggcgtt gaattaaata tttatttaga tgacgataca atatcattta gtctatctcc
     2581 tgtacaaaca gaagttatat ttaaagcatt gggactacaa tttgatgcaa agacacaaac
     2641 attaagttca tttagtgaca acagtttaca aaaacatgtt cttcctaaaa ttaattttgt
     2701 acctaaataa aaagcattac atccattgat gtaatgcttt ttatttatcc tcatattcat
     2761 gaagttcaat cacattttct gaatcaccaa taatacgagc atattctaaa ccaccatcta
     2821 ctcccactga tccgcatgaa caatacttaa aatcatgagt gaatttagat tcaattacgt
     2881 caccacattt tcggcattga attgcatttc tccttagctt tttcattcat aatcctccta
     2941 tcaaagttct cgttcatttc cacgttcttt tctttgttgc ttttgttcag tcgcaagaaa
     3001 ctttgatgct gattcatatc cttttttctc attttcattc aatgcttttt ccgaatacct
     3061 aagagaacta aatctattta tagatccatt tacttttctc ttacaaaaac caattatttc
     3121 atgaaatctc ggaatttctt tcggaataaa cttctcataa aatttattca tttcatcaaa
     3181 taattttgct agacgttcat atttgccctt atatggcttt aacccttcat tttctttttc
     3241 taaacgcttc actgttgtat gtaaatctgt attattcgct tgtaacgctt cattcttacg
     3301 atgtaacgtc atattttcgc gtttaaaggc ttctgatgcc tttgccttag ttttgagttc
     3361 ctcaaagtct tctacgctca tttcaacagt tttagagcgt aaaataccac ctttatgttt
     3421 aacttccatc ttatcaactt tatttgttga tttcactgcg ttttgtaatt gatttaaacg
     3481 agattctaat tgttccattt gtttatttac tacttctttt ttctgtagac attgtccgat
     3541 ttcagtttct agtgctttta tctcttcttt tgcggtcatt gccttgaatt ttcgcatttc
     3601 tacatgcttt ttatcacttg atacaccacg ttccaaataa aagccctttt ctaccatgtg
     3661 agcatgaaaa tcatcctgga gtgaaaccaa ttcttttcgg ttaaaaattt gtttggctga
     3721 taacttattt tcttccgtta ctggaaccat accaacgtgc atatgaggtg ttttctcatc
     3781 caaatgtacc gctgcatgca caatattacg ttcaccatat ctatctttta aaaaactata
     3841 cgcttcttta aaaaatactt tttgttgagc tggatcaacc atatctaata catcaaaaaa
     3901 ttctctatca cttgtaacaa caaattcgca catcacaacc gcatcttttc gaatcgctcg
     3961 attggtctct acattctgtt caattttgtc tttcactgtt ttcaaataat caatgtggcc
     4021 atcattatgt aaatcataat ttagatgtgt tcgctccgat tcaatatcat gatttgtgtg
     4081 actctctttt tctcgctgat tatgaatctg aattcccttc acatctcgca ttttaaattt
     4141 ctgcattcgg caaattgcat aactcatttt ttcgcctctt ttccataggt tctacgtcct
     4201 tatcagtccc tagaaccata taaagtatat atacttctct actgtttttc ggtggacttc
     4261 catgtaaagt atagcacact atactttact atcgtaaagg tgtgccctgc ggggctattc
     4321 ggcaagccga atcaaacctc ggactttgtc cgaacctaca aggggaactc ttccccttga
     4381 ccccctcaat cgaactcccc aacccctcaa tccttgaggg gctccctcgc cgattggcaa
     4441 gaacaaacag tagtttgttc agtgccgaga gggttcgggt atagtccagt tggtcaagtc
     4501 aaatactccc ttcgcttcgc taggtccgtt ttgattgacc aacacacgca acgacctcta
     4561 ttgatcgtct tcattttcct ctcgtttttt tcaccgcata ttcatataat cgttcattaa
     4621 accctttatc tatctttaaa aattgcttct ggggtaccgc cagcatttcg gaaaaaatct
     4681 acgctaagaa catgcgagat tttatacagt tttttggcta atctagtaac aaacgagaac
     4741 ccgaaaaccc acgccaggat aggaatgtat aaaatgatgc atagaataat acaaaaagaa
     4801 aacatacaaa cccaattgta tcaaaggttt gtacgttttc tttgctaccg ataattgtgc
     4861 gttatgtata ctagatttgt ataacgtatt catgtgtttt taataagtca tcttgaatct
     4921 aagtacattc ccttaacaag tgcatagaat aattttgtaa aaacgcgaaa ggattgggaa
     4981 aaatgcacta taatccttat atatacccat atcaatatct ttattatgtt aatgtaccaa
     5041 taccaatgta taactatgga agacagtctg tttaccggac ttttcctaat gagatagagc
     5101 atgcaaacag atttgattct tttcgctctt ctaacaatga cagaagaatt tcattaacag
     5161 attatggacc aaaaccattt gtcgttaata tcaatgaagc aacgaagcaa aacaatacct
     5221 atcgtactgc tttat
//
