LOCUS       CP113384                3124 bp    DNA     circular ENV 13-DEC-2022
DEFINITION  MAG: Bacillus paranthracis isolate 1702 plasmid unnamed9, complete
            sequence.
ACCESSION   CP113384
VERSION     CP113384.1
DBLINK      BioProject: PRJNA865942
            BioSample: SAMN30148863
KEYWORDS    ENV; Metagenome Assembled Genome; MAG.
SOURCE      Bacillus paranthracis (mixed culture metagenome)
  ORGANISM  Bacillus paranthracis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Identification and pathogenic (toxigenic) potential of
            Staphylococcus haemolyticus and Bacillus paranthracis consortium
            isolated from bovine mastitis in Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) Microbiology Laboratory, Federal Research
            Center for Animal Husbandry named after Academy Member L.K. Ernst,
            Dubrovitsy 60, Podolsk Municipal District, Moscow Region 142132,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/29/2022 13:03:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,200
            CDSs (total)                      :: 6,060
            Genes (coding)                    :: 5,841
            CDSs (with protein)               :: 5,841
            Genes (RNA)                       :: 140
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 92 of 219
            Pseudo Genes (incomplete)         :: 115 of 219
            Pseudo Genes (internal stop)      :: 63 of 219
            Pseudo Genes (multiple problems)  :: 40 of 219
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Bacillus paranthracis.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Bacillus paranthracis"
                     /mol_type="genomic DNA"
                     /isolate="1702"
                     /isolation_source="milk"
                     /host="bovine"
                     /db_xref="taxon:2026186"
                     /environmental_sample=""
                     /plasmid="unnamed9"
                     /geo_loc_name="Russia: Saratov region"
                     /collection_date="2020"
                     /metagenome_source="mixed culture metagenome"
                     /note="metagenomic"
     gene            join(2732..3124,1..561)
                     /locus_tag="NRZ51_30990"
     CDS             join(2732..3124,1..561)
                     /locus_tag="NRZ51_30990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WAI41680.1"
                     /translation="MTKKNQDEIVWKYQPKKKQNLKIYEFIKDKLSEGGRELFKECSTF
                     NQFISTRDKIKNKLIGSNPCKNRFCPICAWRKARKDAMKIATMMEAIKVEEKKEFLFLT
                     LTTPNIKADMVKSEIDRFNKAFKKLFDRKKVQRSIKGYIRKLEMTYNKERDDYNPHFHV
                     LLCVDKNYFKRKELYIKQQEWLSMWREATDLPEITQVHIQKVELIREGNAVGEIAKYSA
                     KDYELATNQEVFDVFYASLKGRQLITFNGLFKEYALKYENGELDKYKTKDKNEYFYKIL
                     ATWNPDLLKFNREYTELTEEEKEKYNGHLIDEMEVE"
                     /name="protein rep"
     gene            1818..2186
                     /locus_tag="NRZ51_30995"
     CDS             1818..2186
                     /locus_tag="NRZ51_30995"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI41678.1"
                     /translation="MKKMVQVLLVSFLVLTSVAFVKPSSSYALDCLGRDRPTCAKWDYL
                     RILQKIPKSLKKSNEVVDLKKFSQKIRGTNDYKDPKSGWIISKNKGKSHGGSAWKLYNK
                     GKKERIASLTSSGKVLRE"
                     /name="hypothetical protein"
     gene            2211..2414
                     /locus_tag="NRZ51_31000"
     CDS             2211..2414
                     /locus_tag="NRZ51_31000"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI41679.1"
                     /translation="MAKPLFCPNCGVKLIDEDFYVKSFTSYETKDRNNNVVVIDLNGWG
                     CECASCNWEGDILPYDEDTEYN"
                     /name="hypothetical protein"
ORIGIN
        1 agaaaaaagg ttcaaagaag tattaaaggg tatattagaa aattagagat gacttacaac
       61 aaagaacgtg atgactataa tccgcatttt catgttttac tttgtgtaga taaaaattat
      121 ttcaaacgta aagagcttta cattaaacaa caagaatggc tttcgatgtg gcgagaagct
      181 acggatctac ctgaaataac acaagtacat attcagaaag tggaattgat tcgagaaggg
      241 aatgcagttg gtgagattgc gaagtattca gcgaaagatt atgagctggc aacaaaccaa
      301 gaagtgtttg atgtgtttta tgcatcgttg aaaggccgac aactgattac gtttaatgga
      361 ctgtttaaag agtatgcttt gaagtatgaa aatggtgagt tggacaagta taaaacaaaa
      421 gataaaaacg agtattttta taagatatta gcaacatgga atcctgattt attgaaattt
      481 aatcgtgaat atactgagtt aactgaagaa gaaaaagaaa aatataatgg gcatttgatt
      541 gatgaaatgg aagtggaatg aaaaaaagaa cctggattag ttcctggttc ttttttattt
      601 tttttatgtt aactaaggta tgaatacttt aaatttaatc atcattcttt tgttttatat
      661 tgttgcgaag gtgaatgagt aagaactcca ctgaatatga atctgttctt ttaatttgtg
      721 ttattgcgaa ggtgaacaga aaaaaacttc gctgaatttc gtttttattt taccacaaaa
      781 ttattttttt ggtgcaattt tagtgaccac tgagtgacca ctgagtgcca ctgaatgatc
      841 actagaacga aaaaatgacc acccagtgac cactggataa aaaagaaccg tcccagttac
      901 aacattggtt tcatggtcat ttggtggtca ttttagacgc atacaaaaca catattttca
      961 catacaaaac gcatgcaaat agccgaaaaa cgcatacaga acgcatacaa ccgaaaaaag
     1021 tcttcccaca attcacaaac tagaaaccca ataggcacag cctatttcac catttttttt
     1081 ataaagtggg attcggtttc cttatgctct ttttttattt cttggttttt aaagcgttta
     1141 aaatgattca gaatcgcttt taatgatttt atgtagttga attcattaga aaaaaataaa
     1201 gcgtttaaaa cgaattctga acgttctgag acgtattttt tatatcattc tatgatttct
     1261 ttatggtttt tttagttaga gcaaaaaaat aataagttag tagatcgtaa ggagattttt
     1321 aaaatttgac gagacatagg ggaatttaac gaggcgtggc gaggaaaaaa tgcagggcag
     1381 cggggatgta ttctcggatt ttagcgaggc atagggaggc gtggtgaatg taaacgaaga
     1441 ctctttgata ctttggtttt ttctcgtgaa cggtggaggt ggttatctgg cgtttgtcca
     1501 acggtaagcg gaacgtagtg agcattaagg agtggacgaa cttgctacac tgaagaccct
     1561 cttgttgtct cccaaacttt acgtttgggg gctggctacc gccgagggag tccttccgag
     1621 atcggaaggg ttgggtaggt tgagtttttg tcttacgaat catctaaaaa aaagataatg
     1681 aaattaagat ttaataaagt taaatattcc cttttatata tgtttaattt tactatatat
     1741 agaattcaaa aaccatactt gtattatatt tccattaatc ttagaattta ggaaaaacca
     1801 atgcgaggtg ttttgagatg aaaaaaatgg tacaagtttt attagttagt tttcttgttt
     1861 taacttcagt tgcatttgtt aaaccttctt cttcgtatgc tttagattgt ttgggaagag
     1921 ataggccgac ttgtgcaaaa tgggattatt taagaatact tcaaaagata cctaaatctt
     1981 taaaaaaatc taatgaagtt gttgatttga agaagttttc gcaaaaaatt agaggtacta
     2041 atgattacaa agatcctaag tcaggttgga ttatttctaa aaataagggt aaatcacatg
     2101 gtggttcagc gtggaagttg tataataaag gcaaaaagga acgtattgct tctttaacga
     2161 gtagtggaaa agtgttaaga gaataaaaaa atattaaggg tgattgtttt atggctaaac
     2221 ctttgttttg tcctaattgt ggagttaaat taattgatga agatttttat gtaaagagtt
     2281 ttactagtta tgaaactaaa gatcgtaata acaatgtagt tgtaattgat ttaaatggat
     2341 ggggatgtga atgtgcttcc tgtaattggg aaggtgacat tttaccttac gatgaagaca
     2401 ctgaatataa ttgatttact aaaaaaaagt agagaggaaa acgaatcgtt ttcctctcta
     2461 cttttttgcg attttccgga acggaaaatc gtgttttttc ttttcttgat acaagggtaa
     2521 ctatttcggc ggcgaaatag ttacccttgt atgccttaaa gccttgtggt tgtaaggttt
     2581 ttaataaaaa aagacttgct ttttcaggcg gatagattta acgttttaat tgtcgagaaa
     2641 aaaacaataa actatcacga aaggagcaag tctcttttta aagggttggt gtaccctttt
     2701 ttactattca actgggacta atatatcaga tatgacgaaa aaaaatcaag acgaaattgt
     2761 ttggaagtat caaccgaaga aaaaacagaa tttaaaaatc tatgaattta ttaaagataa
     2821 attgagtgag ggtggacgtg aattgtttaa agagtgtagt acttttaacc aatttatttc
     2881 aactcgtgac aaaataaaaa ataaattaat agggtctaat ccttgtaaaa ataggttttg
     2941 tccgatttgt gcttggagga aagctcgtaa agatgcgatg aaaatagcta cgatgatgga
     3001 agctatcaag gttgaggaaa agaaagagtt tttatttctc actttgacta cgccgaatat
     3061 taaagctgat atggtgaaaa gtgaaattga tagatttaat aaagctttta agaaattgtt
     3121 tgat
//
