LOCUS       CP113383                3562 bp    DNA     circular ENV 13-DEC-2022
DEFINITION  MAG: Bacillus paranthracis isolate 1702 plasmid unnamed8, complete
            sequence.
ACCESSION   CP113383
VERSION     CP113383.1
DBLINK      BioProject: PRJNA865942
            BioSample: SAMN30148863
KEYWORDS    ENV; Metagenome Assembled Genome; MAG.
SOURCE      Bacillus paranthracis (mixed culture metagenome)
  ORGANISM  Bacillus paranthracis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Identification and pathogenic (toxigenic) potential of
            Staphylococcus haemolyticus and Bacillus paranthracis consortium
            isolated from bovine mastitis in Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) Microbiology Laboratory, Federal Research
            Center for Animal Husbandry named after Academy Member L.K. Ernst,
            Dubrovitsy 60, Podolsk Municipal District, Moscow Region 142132,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/29/2022 13:03:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,200
            CDSs (total)                      :: 6,060
            Genes (coding)                    :: 5,841
            CDSs (with protein)               :: 5,841
            Genes (RNA)                       :: 140
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 92 of 219
            Pseudo Genes (incomplete)         :: 115 of 219
            Pseudo Genes (internal stop)      :: 63 of 219
            Pseudo Genes (multiple problems)  :: 40 of 219
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Bacillus paranthracis.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Bacillus paranthracis"
                     /mol_type="genomic DNA"
                     /isolate="1702"
                     /isolation_source="milk"
                     /host="bovine"
                     /db_xref="taxon:2026186"
                     /environmental_sample=""
                     /plasmid="unnamed8"
                     /geo_loc_name="Russia: Saratov region"
                     /collection_date="2020"
                     /metagenome_source="mixed culture metagenome"
                     /note="metagenomic"
     gene            join(2734..3562,1..446)
                     /locus_tag="NRZ51_30975"
     CDS             join(2734..3562,1..446)
                     /locus_tag="NRZ51_30975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016111093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WAI41675.1"
                     /translation="MAHVEKYTKGSVQGLSIHWDRKTENHSNQDIDNERSHLNYDLCEK
                     EGDTLSRMNDRLSEVHCLNRKDVKVCADWAVTLPESLKDASDKEHREFFEKTYEFLINR
                     YGGEKNVLSANVHNDETRPHMHFAFMPVVWDEKKQREKVSAKEVLTRKDLRTFHQDLDE
                     FLKKELPHIYKGGILNDKTIGVDTVKDLKKHSDEIHKQKQQQKADATAEMKVFKEPKEV
                     LKKIEASAKKTMFGDKVTLPSKEFEKLKDLSVSSMKVQYQAEKRMAAANEKIEELEEKY
                     YEADRRADNAEFQFGLLEKEVDTLKEHRTDAIVYKSMLQDINKDLEISDTEKKGRLILH
                     SLENGHKPKNRKEGEQWLSILEENKEAKTIPQKRLEGFIERLKEFLDKILGKERKYSLS
                     GLKRQSEQSKKERRPKTQKNRGMEL"
                     /name="plasmid recombination protein"
     gene            complement(487..711)
                     /locus_tag="NRZ51_30980"
     CDS             complement(487..711)
                     /locus_tag="NRZ51_30980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016111092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI41676.1"
                     /translation="MTANQMLEMIVEQSVQELEKIENLKELHKHEDKFTFEEFEQLEVA
                     ILLEMSNMKEEFYRKGFKQGVKFILSMQK"
                     /name="hypothetical protein"
     gene            complement(847..1335)
                     /locus_tag="NRZ51_30985"
     CDS             complement(847..1335)
                     /locus_tag="NRZ51_30985"
                     /inference="COORDINATES: protein motif:HMM:NF017542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WAI41677.1"
                     /translation="MAKVTNTISRTVVNEDGQITHQEEEKVINWGTEPNYIKVYLDTIL
                     YLKDLPKGLNSILYAFLKRMSYGNQLVVNAALKRQIAKELGLSLSSINNAISKFVKGEL
                     LIREDTGLYKVNPHLFGKGDWKDIAKIRLEVVFDPKRKTIMSEIEKKETKEEKQKVEI"
                     /name="replication/maintenance protein RepL"
     Typing          22..443
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.943"
                     /coverage="94.0"
                     /evalue="8.700000000000001e-70"
     Typing          2735..3561
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="82.971"
                     /coverage="99.0"
                     /evalue="3.79e-155"
ORIGIN
        1 tagaagagaa gtattacgag gcggatcgaa gggcagataa tgcggaattt caatttggtt
       61 tactggaaaa agaagtagac acgctgaagg aacacagaac ggatgcgatt gtatataaga
      121 gcatgttgca agatataaac aaagatttag agattagcga cactgaaaag aaaggacgtt
      181 tgattctcca tagtctagag aatgggcata agcctaaaaa tcgaaaagaa ggcgaacaat
      241 ggctttctat cctggaagag aataaagagg cgaagacaat ccctcagaag cgtttagagg
      301 gctttataga gcgtttaaaa gaattcctag ataaaatact agggaaagag cggaaatact
      361 ctctatcggg cttaaaacgt caatcagagc aatctaaaaa agagagacga ccaaagacac
      421 aaaaaaatag agggatggag ctataaaaaa ggcgataccc agtgtaaggg gcatcgcctt
      481 tttgctctat ttttgcatag ataaaatgaa ttttacgcct tgtttaaagc cttttctata
      541 aaattcttct ttcatgttcg acatttctag aagtattgct acttcaagtt gttcaaattc
      601 ttcaaaagtg aatttgtctt cgtgtttatg taattcctta agattttcaa ttttttctag
      661 ttcctgtaca ctttgctcaa caatcatttc taacatttgg tttgcagtca ttatatttcc
      721 ctccagtaaa tgtatttgat ttataaaaaa ggtttgtgca tactggagac atgcaaatag
      781 gggtctcaat cttatctgca taagtgttct ctgtgtccag tatgcaggga acattttttt
      841 tggggtttat atttctactt tttgtttttc ttctttggtt tctttttttt caatttcaga
      901 cattatagtt tttctttttg gatcaaaaac aacttctaaa cgtatttttg caatgtcttt
      961 ccaatcgcct tttccaaaca aatgaggatt tactttataa agtcctgtat cttccctaat
     1021 tagtaattcg cctttcacaa atttagaaat agcgttatta atacttgata gagaaagtcc
     1081 taattctttt gctatttgtc tttttaatgc cgcattaaca actaattgat ttccataaga
     1141 cattcttttt agaaaagcat ataaaatgct atttaatcct ttaggaaggt cttttaaata
     1201 taaaattgtg tctaaataaa cttttatgta attcggctct gtaccccaat ttataacttt
     1261 ctcttcttct tgatgagtaa tttgaccatc ttcattaacg actgttcttg aaattgtatt
     1321 agtcacttta gccataatag cacctcctta ctataaaata atgtaatgtc attataacga
     1381 accgttgtaa tgattgtaaa gagtaaaaat gcaaaacgtt acaataaatc attgtaatgt
     1441 taattacatc tctcgtgtaa tatgattaca tccctcatgt aatgtcgtta catccctcat
     1501 gtaatgccta aaatcgccaa atcccttgat atatctagct taaacactat ttttctagaa
     1561 actatcccct ctcttatctt gtaccatggt tttggtgttt tgttcgacag tataaaaaaa
     1621 ttggctttta actaagcaat ggacgggtag tattttttga aaaaaccact caaaaaatct
     1681 ccaccctttt tccattgctt agttaaattc ggcgggtttt ggaatgtcgc caagccgaac
     1741 agaccaaaaa cgaggattta agttgctcga cttggactag caattgtacc tgccgaaccg
     1801 ccttgatcta tcgttcaaga atttcactaa tgccatataa aattacgtta agttcctggg
     1861 ggctagcccc caaaccccca gccagcactc acacccaaaa acaaggggga taccaataaa
     1921 aaagtcaaaa tcctatatag tggattttga cttttttatt ggtattgaac tacaagggtc
     1981 cctacaggct gtttttgaac acccgtagtt ctttttaaaa gggattaatt tatcagattt
     2041 attaaatgaa taattcataa aatccccttt caatcactct gttgtatggc aatttatcaa
     2101 gtaggcagtt gtacataagt agtgtatgca ggggacaaaa atgtgtatat aactggataa
     2161 gcctttttta tttttggctt ggtatgaaaa aagaacccta atgttggata gggttctttt
     2221 taggggtaac agttgaaggt tgaaggtata tttcatattt attatgtact ttactcttat
     2281 attcattcga ataggaaaca catatgtttg gagtaatttg caaagcgatt gttggaaaag
     2341 ggaaaattag cggtaagtgt caatggtaag cggactggaa ggggtcagcg gtttgtgctg
     2401 atccatgaaa ggagcattaa ggaattgaca cgtgcaattg attcttcacc ctcttggcct
     2461 gtcaaaacgt caaacgtttt ggcctgccaa ccggcgaggg agtccctcaa ggattgaggg
     2521 gttggggagt tcgattgatg gggtcaaggg gaagagttcc ccttgtaggt tcggacaaag
     2581 tccgaggttt ttttgccatg caaggcatgg tttggcgaag ccaacgagcc tcgcagagca
     2641 cgtccaaatg aaatttggta taacgtgcta gaccaaatta ttggattcct tcaatcctcg
     2701 tgttaaattg gttaccaaga agggggattg ctcgtggctc atgtagaaaa atacacaaaa
     2761 ggaagcgtac aaggattatc aattcattgg gacagaaaaa cagaaaatca ttcgaatcaa
     2821 gatattgata atgaacggtc acatttgaat tatgatttat gcgaaaaaga aggagataca
     2881 ctttctcgta tgaatgatcg gttaagtgaa gtacattgtt tgaatcgaaa agatgtaaaa
     2941 gtttgtgcag attgggctgt tacgttacca gaaagtttga aagatgcttc cgacaaggaa
     3001 catcgtgaat tttttgagaa aacctatgaa tttttaataa accgttatgg gggtgaaaaa
     3061 aatgtgttgt ccgctaacgt tcataatgac gaaacaagac cacacatgca ctttgctttt
     3121 atgcctgttg tttgggacga aaaaaaacaa cgtgaaaagg tttcagctaa agaagtttta
     3181 acaagaaaag atttaagaac atttcatcag gatttagatg aatttttaaa aaaagaactg
     3241 ccacatatct acaaaggtgg cattttaaac gataaaacga ttggtgttga taccgttaag
     3301 gatttaaaaa agcattctga tgaaatacat aaacaaaaac aacaacaaaa agcggatgcg
     3361 accgcagaaa tgaaagtgtt taaagaacca aaagaagtac tgaagaaaat tgaagcatca
     3421 gcaaagaaaa cgatgtttgg agataaggtc acattgccgt ctaaggaatt tgagaaatta
     3481 aaagatttat ctgtatctag catgaaagta caatatcaag ctgaaaaaag aatggctgca
     3541 gcaaatgaaa aaattgaaga gt
//
