LOCUS       CP113382                3899 bp    DNA     circular ENV 13-DEC-2022
DEFINITION  MAG: Bacillus paranthracis isolate 1702 plasmid unnamed7, complete
            sequence.
ACCESSION   CP113382
VERSION     CP113382.1
DBLINK      BioProject: PRJNA865942
            BioSample: SAMN30148863
KEYWORDS    ENV; Metagenome Assembled Genome; MAG.
SOURCE      Bacillus paranthracis (mixed culture metagenome)
  ORGANISM  Bacillus paranthracis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Identification and pathogenic (toxigenic) potential of
            Staphylococcus haemolyticus and Bacillus paranthracis consortium
            isolated from bovine mastitis in Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) Microbiology Laboratory, Federal Research
            Center for Animal Husbandry named after Academy Member L.K. Ernst,
            Dubrovitsy 60, Podolsk Municipal District, Moscow Region 142132,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/29/2022 13:03:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,200
            CDSs (total)                      :: 6,060
            Genes (coding)                    :: 5,841
            CDSs (with protein)               :: 5,841
            Genes (RNA)                       :: 140
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 92 of 219
            Pseudo Genes (incomplete)         :: 115 of 219
            Pseudo Genes (internal stop)      :: 63 of 219
            Pseudo Genes (multiple problems)  :: 40 of 219
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Bacillus paranthracis.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Bacillus paranthracis"
                     /mol_type="genomic DNA"
                     /isolate="1702"
                     /isolation_source="milk"
                     /host="bovine"
                     /db_xref="taxon:2026186"
                     /environmental_sample=""
                     /plasmid="unnamed7"
                     /geo_loc_name="Russia: Saratov region"
                     /collection_date="2020"
                     /metagenome_source="mixed culture metagenome"
                     /note="metagenomic"
     gene            711..1205
                     /locus_tag="NRZ51_30955"
     CDS             711..1205
                     /locus_tag="NRZ51_30955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WAI41672.1"
                     /translation="MEVENNTEKGDEVPKQRTENEIIYNGKSRWRNLDTGEIIETDEVI
                     KKTPRNGFMITYLTAIVQLIDSLGNKKMQVVKYILENMDKSTNTLIITTRELTEKSKVS
                     RQTVIDTLKTLEQAQIITRRTGAIMIHPSLVHRGKDTKEKYLLARFEDFSNNKAPINEV
                     E"
                     /name="replication/maintenance protein RepL"
     gene            1220..1504
                     /locus_tag="NRZ51_30960"
     CDS             1220..1504
                     /locus_tag="NRZ51_30960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WAI41673.1"
                     /translation="MKATGITRKVDELGRIVIPKELRKTLKIDIKDPIEIFTEEDSIIL
                     KKYESNMTCIITGEISSKNVSLFNGKIIVSPEGAELLAKELKKIITPVK"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            complement(1537..2853)
                     /locus_tag="NRZ51_30965"
     CDS             complement(1537..2853)
                     /locus_tag="NRZ51_30965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WAI41674.1"
                     /translation="MNKFAIIHMQKFQISDVQGIQKHNQRQGKSKSNLDIDYSKSEQNY
                     DLLNQQKIRYESTIKQEISERVKRKPRANSVVLSEFVVTASPDYMHSLSLEEQKRYFES
                     SLDFIQKRYGKQNTLYAMVHMDEATPHMHIGVMPITEDNRLSAKDIFTKKELVSLQQDF
                     PLEMREKGFDVERGEGSEKKHLSPQAFKEKQDLEVEVEHLSNVKTHLKTKVVETHNQLQ
                     QTTSYIEKQNETLQKIQQQFLSLDKKVKEKKQEFETFRNQIPDKPVSTSYLREETKTEV
                     TTKLFGKPEITEKKTGNIVVTREQWRDMTEKVNAAVIVKKDYERLQKTDLVKENKELHS
                     AVDEICDSLKESQKRNVGLQKENKQLSTEISSLKAHIRDLQTNIKVLYQQTKQVFKEQF
                     KTFRGLIKNELVGREVENHFEREHMREMKSRQKGYDMER"
                     /name="plasmid recombination protein"
     gene            complement(3350..3631)
                     /locus_tag="NRZ51_30970"
     CDS             complement(3350..3631)
                     /locus_tag="NRZ51_30970"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI41671.1"
                     /translation="MFFINKYSVILFSCLFVLISPLTAFAKAEMNIEQRLFINFENKVS
                     DLVITGMELLSNLDKMNGEISEEELYNLALNVGEEFSTRSKILNQISV"
                     /name="hypothetical protein"
     Typing          749..1205
                     /name="rep_cluster_1950"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.323"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1541..2853)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.785"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttcctgccac cataccctta gaataagtca cttttttaag tggcttattt tttataatgt
       61 attgtgataa actataatcg aaataaaaaa attttcctta actaaatatt ttcaaacaca
      121 tatacatggt gtaagggagt cacactcctt attgaacatg tataaaaaag gctaaaatcc
      181 tttcaccagg attttagcct ttttgtgttt ttccttgttt ttgggtgtga gtgctggctg
      241 gggggttggg ggcctagccc ccaggaactt tacgtaacta catagggagt gaaaatgttg
      301 cttagaacga tagaactggg tggttcggct ggtacaattg ttggtccaag tcgagcaact
      361 taaatccccg tttttggtct gttcggcttg gcgaaagcta aatatcagcc gaatttcgaa
      421 atgcaatgga ggaaaggtgg agattttttg agcgtttttt tcaaaaaata ctacctgacc
      481 attgcatgga gaaaaaccct ttaaagaaaa aacaggatct atatatttaa ggcgattttt
      541 atcgccttaa atatatagat aagagtaaca aagaggttaa aagtgaattt tgagttgata
      601 tatagctttt gagacgtatt ttgcttggtc taatttttta taccttttgg tctaattttt
      661 tatacctttt ggtctaattt tttttgacca tttggtctaa aggtgataaa atggaagtag
      721 aaaataatac agaaaaaggt gatgaagtgc caaaacaaag aactgaaaat gaaataattt
      781 ataacggtaa aagtcgatgg agaaacttag atacaggtga aataatagaa actgatgaag
      841 tcataaagaa aactcctaga aatggtttta tgattacgta tttaacagca attgttcaac
      901 tgatagatag tttaggtaat aaaaaaatgc aagtagtaaa atacatatta gaaaacatgg
      961 acaagtcaac aaatacatta attataacga caagagaatt gactgaaaaa agcaaggtat
     1021 ctagacaaac tgtaatagac acattaaaaa cactagaaca agcccaaatc attactagaa
     1081 gaactggagc aataatgatt cacccaagcc ttgttcatcg tggtaaagac acaaaagaga
     1141 aatacttact tgctagattt gaagatttta gtaataataa agcaccaatt aatgaagttg
     1201 aataaaggag agaataaaaa tgaaagctac tggtataact cgtaaagttg atgaattagg
     1261 acgtatcgta atcccaaaag aattacgtaa aacactaaag attgatatca aagatccaat
     1321 agaaattttc acagaggaag atagtattat acttaaaaaa tatgaatcta acatgacttg
     1381 tataataact ggagaaattt ctagcaaaaa tgtttcttta tttaacggaa aaattattgt
     1441 aagtcctgaa ggcgctgaat tattagcaaa agaattaaaa aaaattatta ctccagtcaa
     1501 ataaaaacgc cctagaatat ctctaaggcg ttttttctaa cgctccatgt catatccttt
     1561 ctgtctactt ttcatttctc tcatatgttc acgttcaaaa tgattttcta cctctcttcc
     1621 aaccaattca tttttaataa gccctctaaa cgtcttaaat tgctctttaa agacttgttt
     1681 cgtttgttga tacaaaacct ttatgtttgt ctgtaaatct cttatatgag cctttaatga
     1741 gcttatttct gtacttaatt gtttgttttc cttttgcaac cctacatttc tcttttgaga
     1801 ttctttaagt gagtcacata tctcatcgac tgcactatgt aattctttgt tttcttttac
     1861 aagatccgtt ttttgcaaac gctcatagtc cttcttaact ataaccgctg catttacttt
     1921 ctctgtcata tctctccatt gctcacgtgt taccacgatg tttcctgttt ttttctctgt
     1981 gatttctggt tttccaaaca gttttgttgt tacttccgtt ttggtttctt ctcgtaaata
     2041 agacgttgac actggcttgt caggaatttg gtttcgaaac gtttcaaatt cctgtttctt
     2101 ttcttttact tttttatcta aactcaaaaa ttgttgctga attttttgta acgtttcatt
     2161 ctgtttttcg atgtagcttg tagtttgttg taattgatta tgagtttcta ctacttttgt
     2221 cttcaaatgc gtttttacat tggataagtg ttccacttct acttctaaat cttgtttttc
     2281 cttaaaggct tgaggcgata aatgtttttt ctcagaacct tctccccgct ctacatcaaa
     2341 acctttttct cgcatttcca acggaaagtc ctgttgtaac gataccagtt cttttttagt
     2401 gaaaatatct ttagctgata aacgattatc ttcagtaatt ggcataacac caatatgcat
     2461 atgtggtgtt gcttcatcca tatgaaccat cgcatacaac gtattttgtt tgccataacg
     2521 cttttgaata aaatctagtg aactctcaaa ataacgtttc tgttcttcta gacttaatga
     2581 atgcatgtaa tcaggcgaag ctgttactac aaattcagaa agtacaacag aatttgcccg
     2641 tggctttcgt tttaccctct cactgatttc ttgtttaatt gtactttcat atctaatttt
     2701 ttgttgattc aacaaatcat aattttgttc acttttcgaa taatcaatgt ccaaatttga
     2761 tttacttttt ccttgtctct gattatgttt ttgaatccct tgcacatcag aaatttgaaa
     2821 cttctgcatg tgaatgattg caaatttgtt catgtttttc cctcacaatt gtcttccgta
     2881 ggaggactac ttttgagcct atcataacat atcgagagag cgaatccaaa gttttggtat
     2941 agcccactat accaaattac atttggtgtg ggctctgcga gaccttgttg gcttcgccaa
     3001 atcacgccac agcgtgataa agacaagggg ctttgcccca caccccacaa ggggaattct
     3061 tccccttgac cccatcaatc gaactcccca acccctcaat ccttgagggg cttcctcgcc
     3121 ggtcggcgtc cccaaaacgg ttcgttttga ggaactgcca agaggatgga gaatcggctg
     3181 cacgtgtcaa ttccttaatg ctcctttcat ggatcagcac aaaccgctga accatttcag
     3241 tccgcttacc attgacactt accgattatt tttcttctcc ccataatcgt ttggccagtt
     3301 gttttaaccg ctcgttaaat ttacaatatc ctcttttata atattaagtc taaacactaa
     3361 tttggttcaa aattttagaa cgtgttgaaa attcctcgcc tacatttaat gctaaattat
     3421 atagttcttc ttcacttatt tcaccattca tcttatctaa attacttaat aattccatgc
     3481 cagtaatcac cagatcagaa actttgtttt caaaatttat aaaaagacgt tgctctatat
     3541 tcatttcagc tttagcaaag gctgttagtg gggaaatcaa aacaaataaa caactgaata
     3601 aaattacact atatttattt ataaaaaaca tgtctatctc cacctgagtt tttattttat
     3661 ctaaccttat gatgttctaa agcaataatt ttattttaaa aatcaaaaag ggcttatttg
     3721 ggtatataca taaatttgta ccctgcatac gttgataaag tgcaaccacc caactggtaa
     3781 ttcccacaaa acttttgaaa ggacctatca tgaacgatgc aatttgcaaa tctttaaaga
     3841 caatcctttg taggaaaaaa catgaacatt taagggctac ctgtagagat ccttgcaag
//
