LOCUS       CP113381                5235 bp    DNA     circular ENV 13-DEC-2022
DEFINITION  MAG: Bacillus paranthracis isolate 1702 plasmid unnamed6, complete
            sequence.
ACCESSION   CP113381
VERSION     CP113381.1
DBLINK      BioProject: PRJNA865942
            BioSample: SAMN30148863
KEYWORDS    ENV; Metagenome Assembled Genome; MAG.
SOURCE      Bacillus paranthracis (mixed culture metagenome)
  ORGANISM  Bacillus paranthracis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Identification and pathogenic (toxigenic) potential of
            Staphylococcus haemolyticus and Bacillus paranthracis consortium
            isolated from bovine mastitis in Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) Microbiology Laboratory, Federal Research
            Center for Animal Husbandry named after Academy Member L.K. Ernst,
            Dubrovitsy 60, Podolsk Municipal District, Moscow Region 142132,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/29/2022 13:03:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,200
            CDSs (total)                      :: 6,060
            Genes (coding)                    :: 5,841
            CDSs (with protein)               :: 5,841
            Genes (RNA)                       :: 140
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 92 of 219
            Pseudo Genes (incomplete)         :: 115 of 219
            Pseudo Genes (internal stop)      :: 63 of 219
            Pseudo Genes (multiple problems)  :: 40 of 219
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Bacillus paranthracis.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Bacillus paranthracis"
                     /mol_type="genomic DNA"
                     /isolate="1702"
                     /isolation_source="milk"
                     /host="bovine"
                     /db_xref="taxon:2026186"
                     /environmental_sample=""
                     /plasmid="unnamed6"
                     /geo_loc_name="Russia: Saratov region"
                     /collection_date="2020"
                     /metagenome_source="mixed culture metagenome"
                     /note="metagenomic"
     gene            join(5111..5235,1..448)
                     /locus_tag="NRZ51_30920"
     CDS             join(5111..5235,1..448)
                     /locus_tag="NRZ51_30920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017673839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WAI41667.1"
                     /translation="MHYNPYIYPYQYLYYVNVPIPMYNYGRQSVYRTFPNEIEHANRFD
                     SFRSSNNDRRISLTDYGPKPFVVNINEATKQNNTYRTALWTGTHLQVTLMSLNVGEDIG
                     LEVHSNVDQFLRIEQGQGIIQMGKNKDNLNFKRNVYDDYAIMIPAGTWHNLTNTGNIPL
                     KLYSIYAPPNHPFGTVHVTKADAMVNE"
                     /name="cupin domain-containing protein"
     gene            638..1378
                     /locus_tag="NRZ51_30925"
     CDS             638..1378
                     /locus_tag="NRZ51_30925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016083310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="WAI41668.1"
                     /translation="MKKVLEREEFVDIGGFQLYTKIFGSDKGTPTVVMDAGYGDYSKAW
                     NEIVSQIAEFTKVLVYDRAGLGKSDSSPNNRVSSEMVKELRTLLKKLQLTPPYIFVGHS
                     FGGVNVRLYSSLYPSEVAGLILVDSTPEDYRERFLPIMPSDFQEAYNKQFIYEGTYDQF
                     MKSLEEVRLHRKTLIDTNVIILSAGKKAFYSTDAQNLWLHMQKELLDLSTNSSLVIAPN
                     SGHYIQKDEPQYLINAVKKVLQIL"
                     /name="alpha/beta hydrolase"
     gene            1545..1718
                     /locus_tag="NRZ51_30930"
     CDS             1545..1718
                     /locus_tag="NRZ51_30930"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI41669.1"
                     /translation="MNKDYFGTNDLSGSARILACPSREPLLCFLSVGLARKAHEPKKLP
                     QGWSKAEKVEIF"
                     /name="hypothetical protein"
     gene            2088..2651
                     /locus_tag="NRZ51_30935"
     CDS             2088..2651
                     /locus_tag="NRZ51_30935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WAI41670.1"
                     /translation="MEKHDENLEYHAETQTLVGQKKRELVDTETGEVIHVDQITKRVYG
                     TKNFWKMYLMDFLTVLGIIDNKQLDVFIYIAENTNQSNNLFIGTYRSISKDVGVGVSTV
                     TRIMKKLQESNFIKKKQNGVYIVNPNIMMKGNDTKRQILLSYYEEDKPLNSIDVLRGKQ
                     KALPEQETTKNESKKLGLETKNND"
                     /name="replication/maintenance protein RepL"
     gene            2644..2838
                     /locus_tag="NRZ51_30940"
     CDS             2644..2838
                     /locus_tag="NRZ51_30940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016132552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI41664.1"
                     /translation="MIEGVELNIYLDDDTISFSLSPVQTEVIFKALGLQFDAKTQTLSS
                     FSDNSLQKHVLPKINFVPK"
                     /name="hypothetical protein"
     gene            complement(2869..3054)
                     /locus_tag="NRZ51_30945"
     CDS             complement(2869..3054)
                     /locus_tag="NRZ51_30945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI41665.1"
                     /translation="MKKLRRNAIQCRKCGDVIESKFTHDFKYCSCGSVGVDGGLEYARI
                     IGDSENVIELHEYEDK"
                     /name="hypothetical protein"
     gene            complement(3069..4295)
                     /locus_tag="NRZ51_30950"
     CDS             complement(3069..4295)
                     /locus_tag="NRZ51_30950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WAI41666.1"
                     /translation="MSYAICRMQKFKMRDVKGIQIHNQREKESHTNHDIESERTHLNYD
                     LHNDGHIDYLKTVKDKIEQNVETNRAIRKDAVVMCEFVVTSDREFFDVLDMVDPAQQKV
                     FFKEAYSFLKDRYGERNIVHAAVHLDEKTPHMHVGMVPVTEENKLSAKQIFNRKELVSL
                     QDDFHAHMVEKGFYLERGVSSDKKHVEMRKFKAMTAKEEIKALETEIGQCLQKKEVVNK
                     QMEQLESRLNQLQNAVKSTNKVDKMEVKHKGGILRSKTVEMSVEDFEELKTKAKASEAF
                     KRENMTLHRKNEALQANNTDLHTTVKRLEKENEGLKPYKGKYERLAKLFDEMNKFYEKF
                     IPKEIPRFHEIIGFCKRKVNGSINRFSSLRYSEKALNENEKKGYESASKFLATEQKQQR
                     KERGNEREL"
                     /name="plasmid recombination protein"
     Typing          2089..2651
                     /name="rep_cluster_1909"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.823"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3124..4295)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.141"
                     /coverage="95.0"
                     /evalue="0.0"
ORIGIN
        1 cagatttgat tcttttcgct cttctaacaa tgacagaaga atttcattaa cagattatgg
       61 accaaaacca tttgtcgtta atatcaatga agcaacgaag caaaacaata cctatcgtac
      121 tgctttatgg acaggaacac atttacaagt tactttaatg agtctcaatg ttggcgaaga
      181 tatcggtttg gaagtacatt ctaacgttga tcaattctta cgtattgaac aaggccaggg
      241 gattattcaa atgggcaaga ataaagataa tttaaacttt aaaagaaatg tctatgatga
      301 ttatgccata atgatacccg ctggaacatg gcataatcta accaatacag gtaatattcc
      361 tctaaaactt tactcgatat atgctcctcc taaccatcca tttggtactg tacatgtaac
      421 aaaagccgat gccatggtaa atgaataaaa ctattaaaaa taataaaaag gatcttagat
      481 tgaagatcct ttttttatgc caataatagg acgttatgtt aacttcacat gcatatggta
      541 ttcaaaatag atttctgatt ttcctttttc atccatgata caatttatta aataatttac
      601 ttgttgtacc ttggaatatg gaaggagtta aatctgaatg aaaaaggttt tagaacgtga
      661 ggaatttgtt gatataggtg gttttcaatt atatacaaaa atatttggaa gtgataaagg
      721 aactccaacc gtagtaatgg acgcaggtta tggtgattac tctaaagctt ggaatgaaat
      781 agtatcacaa attgcggaat tcacaaaagt tctggtctat gatagagctg gtttaggaaa
      841 gagtgattct agtccgaaca atagagttag ttctgaaatg gtaaaagagc taagaacatt
      901 actaaaaaaa ttacaattaa cgcctcctta tatttttgtg gggcattcct ttggtggagt
      961 aaatgttcgc ttatactcat ctttatatcc aagtgaggta gcagggctaa tacttgtgga
     1021 ttcaacccca gaggactaca gagaacgatt tcttcccatc atgccttcag atttccaaga
     1081 agcttataat aaacaattta tttatgaagg tacatacgat cagtttatga agagtctaga
     1141 agaagtacga ttacacagaa aaacacttat agatacaaat gttattattc tttctgcagg
     1201 aaaaaaagct ttttattcca ctgatgcaca aaacttatgg ttgcatatgc aaaaggaatt
     1261 attggattta tcaactaata gttccttagt tatagcacct aacagtggac actatatcca
     1321 aaaggatgag ccacaatatc taataaatgc ggtcaaaaag gttcttcaaa tcttataatt
     1381 aacttccgag ttcgagaatt atgtgaacat gaaagtcccc cagtcaaaca atctggagga
     1441 cttttttatg gtttgttctt gtcttttggt cttacatttg ctttttggct agagtgctgg
     1501 ctcgtcgtgt gggggcgaaa ccccacgtat tacgtatcta ttttttgaat aaggactatt
     1561 ttggaacgaa tgatctaagc ggttcggctc gtatacttgc ttgtccaagt cgagaaccat
     1621 tgctttgctt tttgtctgtc ggcttggcga gaaaagcaca cgagccgaaa aaacttccgc
     1681 aaggatggag caaagcggaa aaggtggaga ttttttgagt gtttttctca aaaaatacta
     1741 cctgttcctt gtagaagttt tagctcttaa ggaaaaaaag aaaaaaagac caactaaaag
     1801 ttggtctttt ttaaaatatc aaaggggcta aatagcccct ttgatattag aagagaggaa
     1861 acgtgcataa aaagtgggat aaacccagtt atatcaacgg atttcacgtt tttgcttgtg
     1921 ttcatatttg atagcaattg tgttcatttt tgaagatgtc tgtattcttt ttgtgaacac
     1981 aattgtattt tttatataag tatgttagtg ttcttataaa gagttatttg gtgttcaggt
     2041 atgaatacac attgaatata tattaaatac atattggggg atatttcatg gaaaaacatg
     2101 acgagaattt agaatatcat gcagaaactc aaacattagt tggtcaaaag aaacgagaat
     2161 tagtagacac tgaaactgga gaagttattc atgtagacca aataaccaaa agagtatatg
     2221 gaactaagaa tttttggaag atgtacttaa tggatttctt aacagttcta ggaatcatag
     2281 acaacaaaca actagatgtt tttatataca tagcggaaaa tacaaaccaa tctaacaatc
     2341 tttttatagg tacatataga agtatctcaa aagatgttgg tgtcggtgta agtacagtga
     2401 cacgtattat gaaaaaacta caagaaagta attttatcaa gaaaaaacaa aatggcgttt
     2461 atatcgtaaa tcctaacatc atgatgaaag ggaacgatac aaaacgacaa atattattaa
     2521 gttattacga agaagataag cctttaaatt ctatcgacgt attaagaggg aaacaaaagg
     2581 ctcttccaga acaagaaaca acaaaaaacg aatcaaaaaa attaggattg gagacgaaaa
     2641 acaatgattg agggcgttga attaaatatt tatttagatg acgatacaat atcatttagt
     2701 ctatctcctg tacaaacaga agttatattt aaagcattgg gactacaatt tgatgcaaag
     2761 acacaaacat taagttcatt tagtgacaac agtttacaaa aacatgttct tcctaaaatt
     2821 aattttgtac ctaaataaaa agcattacat ccattgatgt aatgcttttt atttatcctc
     2881 atattcatga agttcaatca cattttctga atcaccaata atacgagcat attctaaacc
     2941 accatctact cccactgatc cgcatgaaca atacttaaaa tcatgagtga atttagattc
     3001 aattacgtca ccacattttc ggcattgaat tgcatttctc cttagctttt tcattcataa
     3061 tcctcctatc aaagttctcg ttcatttcca cgttcttttc tttgttgctt ttgttcagtc
     3121 gcaagaaact ttgatgctga ttcatatcct tttttctcat tttcattcaa tgctttttcc
     3181 gaatacctaa gagaactaaa tctatttata gatccattta cttttctctt acaaaaacca
     3241 attatttcat gaaatctcgg aatttctttc ggaataaact tctcataaaa tttattcatt
     3301 tcatcaaata attttgctag acgttcatat ttgcccttat atggctttaa cccttcattt
     3361 tctttttcta aacgcttcac tgttgtatgt aaatctgtat tattcgcttg taacgcttca
     3421 ttcttacgat gtaacgtcat attttcgcgt ttaaaggctt ctgatgcctt tgccttagtt
     3481 ttgagttcct caaagtcttc tacgctcatt tcaacagttt tagagcgtaa aataccacct
     3541 ttatgtttaa cttccatctt atcaacttta tttgttgatt tcactgcgtt ttgtaattga
     3601 tttaaacgag attctaattg ttccatttgt ttatttacta cttctttttt ctgtagacat
     3661 tgtccgattt cagtttctag tgcttttatc tcttcttttg cggtcattgc cttgaatttt
     3721 cgcatttcta catgcttttt atcacttgat acaccacgtt ccaaataaaa gcccttttct
     3781 accatgtgag catgaaaatc atcctggagt gaaaccaatt cttttcggtt aaaaatttgt
     3841 ttggctgata acttattttc ttccgttact ggaaccatac caacgtgcat atgaggtgtt
     3901 ttctcatcca aatgtaccgc tgcatgcaca atattacgtt caccatatct atcttttaaa
     3961 aaactatacg cttctttaaa aaatactttt tgttgagctg gatcaaccat atctaataca
     4021 tcaaaaaatt ctctatcact tgtaacaaca aattcgcaca tcacaaccgc atcttttcga
     4081 atcgctcgat tggtctctac attctgttca attttgtctt tcactgtttt caaataatca
     4141 atgtggccat cattatgtaa atcataattt agatgtgttc gctccgattc aatatcatga
     4201 tttgtgtgac tctctttttc tcgctgatta tgaatctgaa ttcccttcac atctcgcatt
     4261 ttaaatttct gcattcggca aattgcataa ctcatttttt cgcctctttt ccataggttc
     4321 tacgtcctta tcagtcccta gaaccatata aagtatatat acttctctac tgtttttcgg
     4381 tggacttcca tgtaaagtat agcacactat actttactat cgtaaaggtg tgccctgcgg
     4441 ggctattcgg caagccgaat caaacctcgg actttgtccg aacctacaag gggaactctt
     4501 ccccttgacc ccctcaatcg aactccccaa cccctcaatc cttgaggggc tccctcgccg
     4561 attggcaaga acaaacagta gtttgttcag tgccgagagg gttcgggtat agtccagttg
     4621 gtcaagtcaa atactccctt cgcttcgcta ggtccgtttt gattgaccaa cacacgcaac
     4681 gacctctatt gatcgtcttc attttcctct cgtttttttc accgcatatt catataatcg
     4741 ttcattaaac cctttatcta tctttaaaaa ttgcttctgg ggtaccgcca gcatttcgga
     4801 aaaaatctac gctaagaaca tgcgagattt tatacagttt tttggctaat ctagtaacaa
     4861 acgagaaccc gaaaacccac gccaggatag gaatgtataa aatgatgcat agaataatac
     4921 aaaaagaaaa catacaaacc caattgtatc aaaggtttgt acgttttctt tgctaccgat
     4981 aattgtgcgt tatgtatact agatttgtat aacgtattca tgtgttttta ataagtcatc
     5041 ttgaatctaa gtacattccc ttaacaagtg catagaataa ttttgtaaaa acgcgaaagg
     5101 attgggaaaa atgcactata atccttatat atacccatat caatatcttt attatgttaa
     5161 tgtaccaata ccaatgtata actatggaag acagtctgtt taccggactt ttcctaatga
     5221 gatagagcat gcaaa
//
