LOCUS       CP113380                7635 bp    DNA     circular ENV 13-DEC-2022
DEFINITION  MAG: Bacillus paranthracis isolate 1702 plasmid unnamed5, complete
            sequence.
ACCESSION   CP113380
VERSION     CP113380.1
DBLINK      BioProject: PRJNA865942
            BioSample: SAMN30148863
KEYWORDS    ENV; Metagenome Assembled Genome; MAG.
SOURCE      Bacillus paranthracis (mixed culture metagenome)
  ORGANISM  Bacillus paranthracis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 7635)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Identification and pathogenic (toxigenic) potential of
            Staphylococcus haemolyticus and Bacillus paranthracis consortium
            isolated from bovine mastitis in Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7635)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) Microbiology Laboratory, Federal Research
            Center for Animal Husbandry named after Academy Member L.K. Ernst,
            Dubrovitsy 60, Podolsk Municipal District, Moscow Region 142132,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/29/2022 13:03:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,200
            CDSs (total)                      :: 6,060
            Genes (coding)                    :: 5,841
            CDSs (with protein)               :: 5,841
            Genes (RNA)                       :: 140
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 92 of 219
            Pseudo Genes (incomplete)         :: 115 of 219
            Pseudo Genes (internal stop)      :: 63 of 219
            Pseudo Genes (multiple problems)  :: 40 of 219
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Bacillus paranthracis.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Bacillus paranthracis"
                     /mol_type="genomic DNA"
                     /isolate="1702"
                     /isolation_source="milk"
                     /host="bovine"
                     /db_xref="taxon:2026186"
                     /environmental_sample=""
                     /plasmid="unnamed5"
                     /geo_loc_name="Russia: Saratov region"
                     /collection_date="2020"
                     /metagenome_source="mixed culture metagenome"
                     /note="metagenomic"
     gene            join(7458..7635,1..107)
                     /locus_tag="NRZ51_30875"
     CDS             join(7458..7635,1..107)
                     /locus_tag="NRZ51_30875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WAI41658.1"
                     /translation="MTANTRHMETQKIERKVTKIGNSLGLTLPQEFYTTLNIKQGDVVS
                     LEINEENEQIVIKKSKKISMPEDLNPKFLESLNRTINKFDGAFKDLVNR"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            145..546
                     /locus_tag="NRZ51_30880"
     CDS             145..546
                     /locus_tag="NRZ51_30880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="WAI41659.1"
                     /translation="MTIYITETQAIFINETLIDMYSPNEQRGVKDTGLLQSAIYRPQQT
                     VAQEDAYPTIFHKATALFESLAKNHAFYNANKRTALACLEMFLLYNEYELKMSEQESSD
                     FTVNVVEQRLSFEEILKIIKENSSKLPLN"
                     /name="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(708..989)
                     /locus_tag="NRZ51_30885"
     CDS             complement(708..989)
                     /locus_tag="NRZ51_30885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002026684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI41663.1"
                     /translation="MEYFHSYGVKFIGTVKELAKKNNLTIEETCRILELTMKIDDYDRK
                     DEQLAGIGELIKEMNDQIMGLSGGLRAISERLRDMNENVVLVSLEDKE"
                     /name="hypothetical protein"
     gene            complement(1021..2226)
                     /locus_tag="NRZ51_30890"
     CDS             complement(1021..2226)
                     /locus_tag="NRZ51_30890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WAI41660.1"
                     /translation="MSKFMVCHMQKFKMTDVKGLQIHNQREKESHSNSDIIQERTEQNY
                     DLIHDKRKVDYKKFIQNRIDNGVVSNRAIRKDAVVCCSFMISASPDYMNSLSQAEQRRF
                     FEESVCFLKARYGEENFVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFKNKSELHDL
                     QDKIYEHVKEKGFDIERGVSSDRKHLSTQRFKAVSLQQEIEKLEQEKKEIDSRLYDLAS
                     SLDQAKSVDEIPVKEKGGFIRSKMVEIASEDFDSIKSLAKSSESLRNENRRLKNEKIKI
                     EREKDDLYKGQRFLERQVTDLKRENRGLKEANDFLKKTLERVKEMYKEKLPELAGVIGY
                     VKGSILDKMNRKFLKRHFAGDDEVKGAQKFLNHKQEHEEQQKRLKQVRRSQQKNWDQGL
                     ER"
                     /name="plasmid recombination protein"
     gene            complement(2637..2849)
                     /locus_tag="NRZ51_30895"
     CDS             complement(2637..2849)
                     /locus_tag="NRZ51_30895"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI41661.1"
                     /translation="MGLGMNIRISVVLPYGYSYSDSNRKCDCINVFGVFERIEKAEALP
                     VKQKNMFVEEVVISLQENSHRSVGR"
                     /name="hypothetical protein"
     gene            complement(3390..3617)
                     /locus_tag="NRZ51_30900"
     CDS             complement(3390..3617)
                     /locus_tag="NRZ51_30900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WAI41662.1"
                     /translation="MKVVNNFRVIMAVKNIDNVAELVRMTGVSRNSINKLWRNDNVSSL
                     RIDTLLPICEKLNVKLSDLIEYIPDDSETK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            3975..4262
                     /locus_tag="NRZ51_30905"
     CDS             3975..4262
                     /locus_tag="NRZ51_30905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI41655.1"
                     /translation="MAYIISKKVKGNIYFYVAQYVGTQPYYSKQYKYKCIYAIGNQKIA
                     LERIAMWLLDNNRIPKELLEIGVSINDVKYWYEKVEKTLQNYSLTNHKNT"
                     /name="hypothetical protein"
     gene            4276..4539
                     /locus_tag="NRZ51_30910"
     CDS             4276..4539
                     /locus_tag="NRZ51_30910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI41656.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPNELIKIGISIDDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     gene            complement(5544..6587)
                     /locus_tag="NRZ51_30915"
     CDS             complement(5544..6587)
                     /locus_tag="NRZ51_30915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WAI41657.1"
                     /translation="MTKKNQDEITGKLQPKKKQNIRIYEFIEKKMSESGRELFKSCSTF
                     NEFVATKDKKKKKRVKGNDCKNRFCPICAWRKAGKDAVKIATMMEAIKIEEKKEFLFLT
                     LTTPNIKADTVKSEIDRFNKAFNKLFKRRNIQRSIKGYIRKLEMTYDKERFITEEMYNN
                     KKRKAYYDSRGLKVDDHNPNYDTYNPHFHVLLCVEKNYFKRKELYIKQEEWLEMWREVT
                     DMPEITQVHIQKVELIREGNAVAEVAKYSAKDYEMSVSQDVFDVFYLALKGRQLIVYGG
                     LLKDYAKKYEDGELDKYKSTDKNEYYYRLIAMWNKDLMKFEQEYQELTESEKQEYNGHL
                     IDEMEVE"
                     /name="protein rep"
     Typing          complement(5545..6722)
                     /name="rep_cluster_617"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1025..2226)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccaaaaaaat ttctatgcca gaggatctca atcctaagtt cttggaatcg ttaaacagaa
       61 ccatcaataa atttgacggt gcatttaaag atttggtaaa cagataatta tccttcaggc
      121 gtggaggaaa gtaggaaaac cgaaatgaca atttatataa cagaaactca agctattttt
      181 atcaatgaaa cacttatcga catgtattca cctaatgaac aaagaggtgt taaagataca
      241 gggttattac aatcagcaat ataccgtcca caacaaacag tagcacaaga ggatgcatat
      301 ccgactatct tccacaaagc cactgcatta tttgaatcat tggctaaaaa ccacgcattc
      361 tataatgcaa acaaaagaac ggcattagct tgcctagaaa tgtttctgtt atacaatgag
      421 tacgaattaa aaatgtccga acaagaatct tcagatttca ctgttaatgt agtagaacag
      481 agattatctt ttgaagaaat cttaaaaatt ataaaagaaa actcatcaaa attaccttta
      541 aattagaaaa agacgtggga tttaaccacg tcttttttat taaacaaact atcttcaagg
      601 atgttataca aggctctaca atcctcgcta tattatgcaa aacaagttcc gctaacgcta
      661 cacatctaaa gaaaaaagag actcacacag agcctcctaa catgccatta ttctttatct
      721 tccaaagata ctaatacaac attttcattc atatcacgca aacgttcact tatagctctc
      781 agacctccac taagtcccat aatttgatca ttcatttcct tgataagttc tccaattcca
      841 gctaattgtt catccttacg atcataatca tcaattttca tcgtcaattc caaaatccta
      901 catgtctctt caatcgttaa attatttttc ttagccaatt cctttacagt acctataaac
      961 ttaactccat aactatgaaa atattccatc cttggttgtt gattaaataa tgttcccaca
     1021 ctatctctcc aatccctggt cccaattttt ctgttggctt cttcgtactt gttttaatct
     1081 cttttgttgt tcttcatgct cttgtttgtg attcaagaac ttttgagcac cctttacttc
     1141 atcatcacca gcaaaatgtc gttttaaaaa cttgcgattc atcttgtcca gtatactccc
     1201 cttaacataa ccaatcaccc ctgcgagttc aggaagcttc tccttataca tctctttcac
     1261 tcgctccaac gttttcttaa gaaaatcatt tgcttccttc aaccctcgat tctctctttt
     1321 caagtctgtt acttgccttt ctaaaaaacg ttgcccctta tataaatcat ctttttcacg
     1381 ttcaatctta atcttttcat ttttaaggcg tctattttcg tttctaagag actcagaaga
     1441 cttagctaat gattttatac tatcgaagtc ttctgaagca atctcgacca ttttagagcg
     1501 tatgaacccg cctttttctt tgactggaat ctcatctaca cttttcgctt gatctaaaga
     1561 cgatgctaaa tcataaagac gactatctat ttctttcttt tcctgctcta acttctctat
     1621 ttcttgttgc aaactcaccg ctttaaaccg ctgcgtcgat aaatgtttcc tgtcgctaga
     1681 aacaccacgc tctatatcaa atcccttttc cttcacatgt tcatagattt tatcttgtag
     1741 atcatgaagc tctgatttgt ttttaaaaaa tgaatacgct gatagttttt gtttttcatt
     1801 aaccggaacc atgcccacat gcatatgagg tgttttttca tcaacatgta ctgaagcata
     1861 tacaaagttc tcttctccat atctcgcctt taaaaaacaa acagactctt caaagaatcg
     1921 tctctgttcc gcttgagata aagaattcat ataatcagga ctagccgaaa tcataaacga
     1981 acaacaaaca acagcatctt tccgaatcgc acgattagat acaacaccat tatcaattcg
     2041 attttgaatg aatttcttat aatccacttt tcttttatca tgaataagat cataattctg
     2101 ttccgttcgt tcttgaataa tgtctgaatt tgaatgactt tctttttcac gctgattatg
     2161 tatttgtaat cctttcacat ctgtcatctt gaatttttgc atatgacaaa ccatgaattt
     2221 tgacatgggc atcgctcctt cttcggtcgt agttccatgt aaactgtagc acactacagt
     2281 ttactgcgta aacacgtgtg ccctacggga catttcggcg accgccgagc catgacaggc
     2341 atggcaaaag aattatgggc tttgcccaaa cccacaaggg aaactcttcc ccttgacccc
     2401 tttcaatcga actccccaac ccctcaatcc ttgagggact ccctcgccgg taggcatccc
     2461 caaaacatgg ttttgggaag aacaagaggg tcttggtcga agttacctat caactcctta
     2521 atgctccatc atttcgctat cgcttcattc cgtccgctta ccgttgacag gtaccataaa
     2581 tactcctccc ccttttaaaa tctgacatcc atatattaaa aagtctcttt ttatattcac
     2641 cgccccaccg atctatggga attttcttgt agtgaaataa ctacttcctc tacaaacata
     2701 tttttttgtt ttacaggaag agcttcagct ttttctattc tttcaaacac cccaaaaaca
     2761 ttaatacaat cacattttct attagaatca ctataagaat agccataggg taaaactaca
     2821 gatattcgta tattcattcc tagacccatt caaataaaaa agagcataag gaaaccgaat
     2881 cccactttgt acaaaaagtg gtgaaataga ctgtgcctat tgggtttctc atgctctact
     2941 tcagggaagc gattttccct aaagtatgcg tcctctatac gtttaaaaac gtttagtatg
     3001 tattctgcat gtgctttgca tacaccatga caaataaaaa aagagcccca caaataggaa
     3061 ctcttttttc attactcatc aattccacca tttcatttta ctgcattacg ataatcacac
     3121 ataaagttaa acaaaatcac gaaatatctg cctacctctc atcttccaag atgttgacgc
     3181 acctagcaag cctataattt catttaccat attcatgtga taacaaacag aaaaagaaag
     3241 aaaaagacag tcataaaagt ttgccgaaat ttaaaataag catataaccc taccaaaaac
     3301 catatgcgaa tataacctgt tttccactag caaatattaa taccgatttc atcatacaca
     3361 agctatctaa agatagaata gcccctaaat tattttgtct cactatcgtc tggaatatat
     3421 tcaattaaat ccgatagttt aacatttaac ttttcacaga tcggtagcaa tgtatctatt
     3481 cgtaaagatg acacattgtc gttacgccac aacttattga tagagttacg acttacccct
     3541 gtcatcctaa ccaactctgc aacattatct atattcttta ccgccataat cacacggaag
     3601 ttatttacta ctttcatttt ttatactcct ttttctcatg aaatatacat gcttttttct
     3661 tataagggca cttttgtgca aagaatagtt gttttttaac cttttaaaca caactttagc
     3721 actttataaa acatttttaa acctgtatgg aataaaaaat catattatgc actatttcat
     3781 acttcattta aattaaagtt aatttatata catataaggt tatttatttt atttatttaa
     3841 actaatttaa aacctattta aattaaaacg ttcaaaatat gcattatttc atactttatt
     3901 tgttgaaaaa ttaacctgat aaagttaata ttaatcaaag taatatagat ttttaatgat
     3961 tggaggtcat ttctttggca tatataatat caaaaaaagt aaaaggaaac atttactttt
     4021 atgtagccca atacgttgga acacaaccat attactcaaa gcaatacaaa tataaatgca
     4081 tttacgcaat cggcaatcaa aaaatagctt tagaaagaat cgcaatgtgg ctattagata
     4141 acaaccgtat accaaaagaa ctactagaaa tcggagtatc cataaacgat gtgaaatatt
     4201 ggtatgaaaa agttgaaaaa acattacaga actactcttt aactaaccat aaaaacacct
     4261 gaaaggagca ttactatggc atttctaaca aattacaaag caaacggaaa aagatacttt
     4321 tatgtagaaa aatatgttgg taaaaaacca tatacctgca aacaaagtga acgtatttat
     4381 tcgattggaa acgaaagaat cacattagaa aggctcactt tatggatttt agataacagt
     4441 ttcattccaa acgaattaat aaagatcgga atatcaattg acgatataga gaactggaga
     4501 gaaaaagtag aaaacaccat aaagagatac tctttataaa taaaactaaa gaaaaaagac
     4561 tgatattttc atcagtcttt tttctttaac tatttatatt ttttcatcat aaaagggtta
     4621 ggtattttaa taatatattt atatatttaa taaaggttac gcgtggcttt ttgaattttt
     4681 catttgcgca caaaagcaaa aagattttaa gagtaaaaag gattaaaaat atacaggaat
     4741 cacgaaataa aaaattcaca gaaacaacaa attcaaacat aatctaaaca tcttttttta
     4801 tcacacaact tgtttaaaac ggtgcaactt tttaacgata tgaaatatct atgaattaaa
     4861 tatgcgagca aagttttttc gttttcagtt cgtcatagag gtactatata aactccctaa
     4921 tttcggaata cagaattaca gagaaaacgg aaaacaaaaa tttcaaaaat cgaaaaatca
     4981 agatttcaaa tttaaaaatt tttaaataaa atcccacacc aaaaatccaa aatcaacccc
     5041 aaaaatgtag aatttctatt ttcaaaaaac atcaaaaaac gtgcggtatt ttatcgatat
     5101 agatatatct aaaaaaattc atgcgcacac tataggtttt taactcgtac agtagtaaaa
     5161 cgaaatcatc agaacaaaat atatctcgaa ataaacttta aaaataaatt cacaaattca
     5221 caaatttaaa tttaagaaaa aactcttttt tgttaattct ctgttagatc ataaaaacct
     5281 tttgaatgct acttgctgca ctcagaaggt tttctttcaa tacaaaaaag agagaaccaa
     5341 acttaatatt aagactggtt ctctctttgt cattcaaata tcatcaaccg caccacattt
     5401 ttcacattta acctagcact tatatgagta atatacatct tcaccatctt tgaccttact
     5461 ttttgaaatc cgataaattt tcatttcttc gttataacat tcccaacagg tatgtttaac
     5521 aatatcacct tttttataca aaactattca acctccattt catcaatcaa atgaccgtta
     5581 tattcttgtt tctcactttc cgttaactct tgatattctt gttcaaattt cattaaatcc
     5641 ttattccaca ttgcaattaa tctataataa tactcatttt tatctgtact cttatacttg
     5701 tccaattcgc catcttcgta ttttttagca taatctttta gcaatccacc gtacacaatt
     5761 aattgtcgac cttttaaagc taaataaaac acgtcgaaca catcttgact cactgacatt
     5821 tcataatctt ttgctgaata cttcgcaacc tctgcaactg catttccttc tcgaattaac
     5881 tctacttttt gaatatgtac ttgcgtaatc tctggcatat ctgttacttc tcgccacatt
     5941 tccaaccatt cttcctgctt aatataaagc tctttacgtt taaaataatt tttttcaaca
     6001 caaagtaata catggaaatg aggattatat gtatcataat tcgggttatg atcgtctact
     6061 ttcaatccac gagaatcata atatgctttt ctctttttgt tgttatacat ttcttcagtg
     6121 atgaatcgtt ctttatcata cgtcatttct agctttctaa tataaccctt aatagaacgt
     6181 tgaatattac gtctcttaaa taatttatta aaagctttat taaatctatc aatctcgctt
     6241 tttaccgtat cagctttaat attcggagtt gtcaatgtta agaaaagaaa ctctttcttt
     6301 tcttcaatct tgattgcttc catcattgtt gcgattttca ccgcgtcctt ccctgctttt
     6361 ctccaagcac aaatcggaca aaatcgattt ttacaatcat taccctttac tcgtttcttt
     6421 ttctttttat ctttcgtagc cacaaactca ttaaaagtac tgcacgattt aaacaattct
     6481 ctcccgctct cactcatttt cttctctata aattcataaa ttcttatatt ctgtttcttt
     6541 ttaggttgta atttaccagt gatttcatct tgatttttct ttgtcatgtt tgatatatta
     6601 gtcctatatg aattgcttaa aaaagggtac acgaatccct ttttcggact tgctccttcc
     6661 ttggtagtta ttgtttttaa ttcgacacta aaaatgataa atctacccgc cggaaatagc
     6721 aagtcttttt tttcgaaatc ccttgtgtat caagggataa aagaatacat aggcatcaat
     6781 ttccgtgcgg aaatagttac ctatgtatta aagaaagaag aaaaccgtat ttttcgtgtc
     6841 cgaaaaacca taaaaaaatg cccttcaatc cacttcgtgg acaagggcat tttttctttt
     6901 aaaaacaaaa attattccta tctctcagcc tcttcatttt cacttctaag cgtttttcaa
     6961 acaacttata atattttctc tttaagaatt acacatcaat cccctatacc tcaaaaacat
     7021 atatttcaga acacgttcga agtgttgcca tgaaaaaatc gagaaattct cgaactttac
     7081 ctgctaatta gaataacagg taactccctt tacactttct ttgtctaaat aaaacaaatt
     7141 caagcaaggc tctacaatct tccctatctt ttgcaatgaa atataaattt gtttacatat
     7201 ttctaaaaat gagtataatt gtattgacgg ttaatacgtt ataactgtta taacattcta
     7261 actaaacgtc ataataatac agaaatacta aacacaacaa acaaaagaga aaactaagaa
     7321 gaccgaaaaa accaaaagca aacaaacaaa acaaacataa aatcacacta tataattcga
     7381 aaaactcaat acaaacacaa agtggcaatt agactacgac aaaaaatagt gtggcaaatt
     7441 ccaaggagga agtccaaatg acagcaaaca ctcgccacat ggaaacacaa aaaatcgaac
     7501 gtaaagttac taaaatcggc aattctttag gtttaacatt accgcaagaa ttttacacta
     7561 ctctaaatat caaacaaggt gacgttgtaa gtttagaaat taacgaagaa aacgaacaaa
     7621 tcgttataaa aaaat
//
