LOCUS       CP113374                3124 bp    DNA     circular ENV 13-DEC-2022
DEFINITION  MAG: Bacillus paranthracis isolate 4M plasmid unnamed10, complete
            sequence.
ACCESSION   CP113374
VERSION     CP113374.1
DBLINK      BioProject: PRJNA865942
            BioSample: SAMN30148851
KEYWORDS    ENV; Metagenome Assembled Genome; MAG.
SOURCE      Bacillus paranthracis (mixed culture metagenome)
  ORGANISM  Bacillus paranthracis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Identification and pathogenic (toxigenic) potential of
            Staphylococcus haemolyticus and Bacillus paranthracis consortium
            isolated from bovine mastitis in Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) Microbiology Laboratory, Federal Research
            Center for Animal Husbandry named after Academy Member L.K. Ernst,
            Dubrovitsy 60, Podolsk Municipal District, Moscow Region 142132,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/30/2022 12:02:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,200
            CDSs (total)                      :: 6,052
            Genes (coding)                    :: 5,834
            CDSs (with protein)               :: 5,834
            Genes (RNA)                       :: 148
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 91 of 218
            Pseudo Genes (incomplete)         :: 114 of 218
            Pseudo Genes (internal stop)      :: 62 of 218
            Pseudo Genes (multiple problems)  :: 38 of 218
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Bacillus paranthracis.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Bacillus paranthracis"
                     /mol_type="genomic DNA"
                     /isolate="4M"
                     /isolation_source="milk"
                     /host="bovine"
                     /db_xref="taxon:2026186"
                     /environmental_sample=""
                     /plasmid="unnamed10"
                     /geo_loc_name="Russia: Moscow region"
                     /collection_date="2020"
                     /metagenome_source="mixed culture metagenome"
                     /note="metagenomic"
     gene            complement(join(2643..3124,1..472))
                     /locus_tag="NRZ50_30990"
     CDS             complement(join(2643..3124,1..472))
                     /locus_tag="NRZ50_30990"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WAI30028.1"
                     /translation="MTKKNQDEIVWKYQPKKKQNLKIYEFIKDKLSEGGRELFKECSTF
                     NQFISTRDKIKNKLIGSNPCKNRFCPICAWRKARKDAMKIATMMEAIKVEEKKEFLFLT
                     LTTPNIKADMVKSEIDRFNKAFKKLFDRKKVQRSIKGYIRKLEMTYNKERDDYNPHFHV
                     LLCVDKNYFKRKELYIKQQEWLSMWREATDLPEITQVHIQKVELIREGNAVGEIAKYSA
                     KDYELATNQEVFDVFYASLKGRQLITFNGLFKEYALKYENGELDKYKTKDKNEYFYKIL
                     ATWNPDLLKFNREYTELTEEEKEKYNGHLIDEMEVE"
                     /name="protein rep"
     gene            complement(790..993)
                     /locus_tag="NRZ50_30995"
     CDS             complement(790..993)
                     /locus_tag="NRZ50_30995"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI30029.1"
                     /translation="MAKPLFCPNCGVKLIDEDFYVKSFTSYETKDRNNNVVVIDLNGWG
                     CECASCNWEGDILPYDEDTEYN"
                     /name="hypothetical protein"
     gene            complement(1018..1386)
                     /locus_tag="NRZ50_31000"
     CDS             complement(1018..1386)
                     /locus_tag="NRZ50_31000"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI30030.1"
                     /translation="MKKMVQVLLVSFLVLTSVAFVKPSSSYALDCLGRDRPTCAKWDYL
                     RILQKIPKSLKKSNEVVDLKKFSQKIRGTNDYKDPKSGWIISKNKGKSHGGSAWKLYNK
                     GKKERIASLTSSGKVLRE"
                     /name="hypothetical protein"
ORIGIN
        1 tatagtcatc acgttctttg ttgtaagtca tctctaattt tctaatatac cctttaatac
       61 ttctttgaac cttttttcta tcaaacaatt tcttaaaagc tttattaaat ctatcaattt
      121 cacttttcac catatcagct ttaatattcg gcgtagtcaa agtgagaaat aaaaactctt
      181 tcttttcctc aaccttgata gcttccatca tcgtagctat tttcatcgca tctttacgag
      241 ctttcctcca agcacaaatc ggacaaaacc tatttttaca aggattagac cctattaatt
      301 tattttttat tttgtcacga gttgaaataa attggttaaa agtactacac tctttaaaca
      361 attcacgtcc accctcactc aatttatctt taataaattc atagattttt aaattctgtt
      421 ttttcttcgg ttgatacttc caaacaattt cgtcttgatt ttttttcgtc atatctgata
      481 tattagtccc agttgaatag taaaaaaggg tacaccaacc ctttaaaaag agacttgctc
      541 ctttcgtgat agtttattgt ttttttctcg acaattaaaa cgttaaatct atccgcctga
      601 aaaagcaagt ctttttttat taaaaacctt acaaccacaa ggctttaagg catacaaggg
      661 taactatttc gccgccgaaa tagttaccct tgtatcaaga aaagaaaaaa cacgattttc
      721 cgttccggaa aatcgcaaaa aagtagagag gaaaacgatt cgttttcctc tctacttttt
      781 tttagtaaat caattatatt cagtgtcttc atcgtaaggt aaaatgtcac cttcccaatt
      841 acaggaagca cattcacatc cccatccatt taaatcaatt acaactacat tgttattacg
      901 atctttagtt tcataactag taaaactctt tacataaaaa tcttcatcaa ttaatttaac
      961 tccacaatta ggacaaaaca aaggtttagc cataaaacaa tcacccttaa tattttttta
     1021 ttctcttaac acttttccac tactcgttaa agaagcaata cgttcctttt tgcctttatt
     1081 atacaacttc cacgctgaac caccatgtga tttaccctta tttttagaaa taatccaacc
     1141 tgacttagga tctttgtaat cattagtacc tctaattttt tgcgaaaact tcttcaaatc
     1201 aacaacttca ttagattttt ttaaagattt aggtatcttt tgaagtattc ttaaataatc
     1261 ccattttgca caagtcggcc tatctcttcc caaacaatct aaagcatacg aagaagaagg
     1321 tttaacaaat gcaactgaag ttaaaacaag aaaactaact aataaaactt gtaccatttt
     1381 tttcatctca aaacacctcg cattggtttt tcctaaattc taagattaat ggaaatataa
     1441 tacaagtatg gtttttgaat tctatatata gtaaaattaa acatatataa aagggaatat
     1501 ttaactttat taaatcttaa tttcattatc ttttttttag atgattcgta agacaaaaac
     1561 tcaacctacc caacccttcc gatctcggaa ggactccctc ggcggtagcc agcccccaaa
     1621 cgtaaagttt gggagacaac aagagggtct tcagtgtagc aagttcgtcc actccttaat
     1681 gctcactacg ttccgcttac cgttggacaa acgccagata accacctcca ccgttcacga
     1741 gaaaaaacca aagtatcaaa gagtcttcgt ttacattcac cacgcctccc tatgcctcgc
     1801 taaaatccga gaatacatcc ccgctgccct gcattttttc ctcgccacgc ctcgttaaat
     1861 tcccctatgt ctcgtcaaat tttaaaaatc tccttacgat ctactaactt attatttttt
     1921 tgctctaact aaaaaaacca taaagaaatc atagaatgat ataaaaaata cgtctcagaa
     1981 cgttcagaat tcgttttaaa cgctttattt ttttctaatg aattcaacta cataaaatca
     2041 ttaaaagcga ttctgaatca ttttaaacgc tttaaaaacc aagaaataaa aaaagagcat
     2101 aaggaaaccg aatcccactt tataaaaaaa atggtgaaat aggctgtgcc tattgggttt
     2161 ctagtttgtg aattgtggga agactttttt cggttgtatg cgttctgtat gcgtttttcg
     2221 gctatttgca tgcgttttgt atgtgaaaat atgtgttttg tatgcgtcta aaatgaccac
     2281 caaatgacca tgaaaccaat gttgtaactg ggacggttct tttttatcca gtggtcactg
     2341 ggtggtcatt ttttcgttct agtgatcatt cagtggcact cagtggtcac tcagtggtca
     2401 ctaaaattgc accaaaaaaa taattttgtg gtaaaataaa aacgaaattc agcgaagttt
     2461 ttttctgttc accttcgcaa taacacaaat taaaagaaca gattcatatt cagtggagtt
     2521 cttactcatt caccttcgca acaatataaa acaaaagaat gatgattaaa tttaaagtat
     2581 tcatacctta gttaacataa aaaaaataaa aaagaaccag gaactaatcc aggttctttt
     2641 tttcattcca cttccatttc atcaatcaaa tgcccattat atttttcttt ttcttcttca
     2701 gttaactcag tatattcacg attaaatttc aataaatcag gattccatgt tgctaatatc
     2761 ttataaaaat actcgttttt atcttttgtt ttatacttgt ccaactcacc attttcatac
     2821 ttcaaagcat actctttaaa cagtccatta aacgtaatca gttgtcggcc tttcaacgat
     2881 gcataaaaca catcaaacac ttcttggttt gttgccagct cataatcttt cgctgaatac
     2941 ttcgcaatct caccaactgc attcccttct cgaatcaatt ccactttctg aatatgtact
     3001 tgtgttattt caggtagatc cgtagcttct cgccacatcg aaagccattc ttgttgttta
     3061 atgtaaagct ctttacgttt gaaataattt ttatctacac aaagtaaaac atgaaaatgc
     3121 ggat
//
