LOCUS       CP113373                3562 bp    DNA     circular ENV 13-DEC-2022
DEFINITION  MAG: Bacillus paranthracis isolate 4M plasmid unnamed9, complete
            sequence.
ACCESSION   CP113373
VERSION     CP113373.1
DBLINK      BioProject: PRJNA865942
            BioSample: SAMN30148851
KEYWORDS    ENV; Metagenome Assembled Genome; MAG.
SOURCE      Bacillus paranthracis (mixed culture metagenome)
  ORGANISM  Bacillus paranthracis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Identification and pathogenic (toxigenic) potential of
            Staphylococcus haemolyticus and Bacillus paranthracis consortium
            isolated from bovine mastitis in Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) Microbiology Laboratory, Federal Research
            Center for Animal Husbandry named after Academy Member L.K. Ernst,
            Dubrovitsy 60, Podolsk Municipal District, Moscow Region 142132,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/30/2022 12:02:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,200
            CDSs (total)                      :: 6,052
            Genes (coding)                    :: 5,834
            CDSs (with protein)               :: 5,834
            Genes (RNA)                       :: 148
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 91 of 218
            Pseudo Genes (incomplete)         :: 114 of 218
            Pseudo Genes (internal stop)      :: 62 of 218
            Pseudo Genes (multiple problems)  :: 38 of 218
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Bacillus paranthracis.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Bacillus paranthracis"
                     /mol_type="genomic DNA"
                     /isolate="4M"
                     /isolation_source="milk"
                     /host="bovine"
                     /db_xref="taxon:2026186"
                     /environmental_sample=""
                     /plasmid="unnamed9"
                     /geo_loc_name="Russia: Moscow region"
                     /collection_date="2020"
                     /metagenome_source="mixed culture metagenome"
                     /note="metagenomic"
     gene            complement(join(2603..3562,1..315))
                     /locus_tag="NRZ50_30975"
     CDS             complement(join(2603..3562,1..315))
                     /locus_tag="NRZ50_30975"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WAI30026.1"
                     /translation="MAHVEKYTKGSVQGLSIHWDRKTENHSNQDIDNERSHLNYDLCEK
                     EGDTLSRMNDRLSEVHCLNRKDVKVCADWAVTLPESLKDASDKEHREFFEKTYEFLINR
                     YGGEKNVLSANVHNDETRPHMHFAFMPVVWDEKKQREKVSAKEVLTRKDLRTFHQDLDE
                     FLKKELPHIYKGGILNDKTIGVDTVKDLKKHSDEIHKQKQQQKADATAEMKVFKEPKEV
                     LKKIEASAKKTMFGDKVTLPSKEFEKLKDLSVSSMKVQYQAEKRMAAANEKIEELEEKY
                     YEADRRADNAEFQFGLLEKEVDTLKEHRTDAIVYKSMLQDINKDLEISDTEKKGRLILH
                     SLENGHKPKNRKEGEQWLSILEENKEAKTIPQKRLEGFIERLKEFLDKILGKERKYSLS
                     GLKRQSEQSKKERRPKTQKNRGMEL"
                     /name="plasmid recombination protein"
     gene            1714..2202
                     /locus_tag="NRZ50_30980"
     CDS             1714..2202
                     /locus_tag="NRZ50_30980"
                     /inference="COORDINATES: protein motif:HMM:NF017542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WAI30027.1"
                     /translation="MAKVTNTISRTVVNEDGQITHQEEEKVINWGTEPNYIKVYLDTIL
                     YLKDLPKGLNSILYAFLKRMSYGNQLVVNAALKRQIAKELGLSLSSINNAISKFVKGEL
                     LIREDTGLYKVNPHLFGKGDWKDIAKIRLEVVFDPKRKTIMSEIEKKETKEEKQKVEI"
                     /name="replication/maintenance protein RepL"
     gene            2338..2562
                     /locus_tag="NRZ50_30985"
     CDS             2338..2562
                     /locus_tag="NRZ50_30985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016111092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI30025.1"
                     /translation="MTANQMLEMIVEQSVQELEKIENLKELHKHEDKFTFEEFEQLEVA
                     ILLEMSNMKEEFYRKGFKQGVKFILSMQK"
                     /name="hypothetical protein"
     Typing          complement(2..315)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="82.857"
                     /coverage="99.0"
                     /evalue="2.27e-50"
     Typing          complement(2607..3562)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.699"
                     /coverage="96.0"
                     /evalue="7.46e-173"
ORIGIN
        1 ataacggttt attaaaaatt cataggtttt ctcaaaaaat tcacgatgtt ccttgtcgga
       61 agcatctttc aaactttctg gtaacgtaac agcccaatct gcacaaactt ttacatcttt
      121 tcgattcaaa caatgtactt cacttaaccg atcattcata cgagaaagtg tatctccttc
      181 tttttcgcat aaatcataat tcaaatgtga ccgttcatta tcaatatctt gattcgaatg
      241 attttctgtt tttctgtccc aatgaattga taatccttgt acgcttcctt ttgtgtattt
      301 ttctacatga gccacgagca atcccccttc ttggtaacca atttaacacg aggattgaag
      361 gaatccaata atttggtcta gcacgttata ccaaatttca tttggacgtg ctctgcgagg
      421 ctcgttggct tcgccaaacc atgccttgca tggcaaaaaa acctcggact ttgtccgaac
      481 ctacaagggg aactcttccc cttgacccca tcaatcgaac tccccaaccc ctcaatcctt
      541 gagggactcc ctcgccggtt ggcaggccaa aacgtttgac gttttgacag gccaagaggg
      601 tgaagaatca attgcacgtg tcaattcctt aatgctcctt tcatggatca gcacaaaccg
      661 ctgacccctt ccagtccgct taccattgac acttaccgct aattttccct tttccaacaa
      721 tcgctttgca aattactcca aacatatgtg tttcctattc gaatgaatat aagagtaaag
      781 tacataataa atatgaaata taccttcaac cttcaactgt tacccctaaa aagaacccta
      841 tccaacatta gggttctttt ttcataccaa gccaaaaata aaaaaggctt atccagttat
      901 atacacattt ttgtcccctg catacactac ttatgtacaa ctgcctactt gataaattgc
      961 catacaacag agtgattgaa aggggatttt atgaattatt catttaataa atctgataaa
     1021 ttaatccctt ttaaaaagaa ctacgggtgt tcaaaaacag cctgtaggga cccttgtagt
     1081 tcaataccaa taaaaaagtc aaaatccact atataggatt ttgacttttt tattggtatc
     1141 ccccttgttt ttgggtgtga gtgctggctg ggggtttggg ggctagcccc caggaactta
     1201 acgtaatttt atatggcatt agtgaaattc ttgaacgata gatcaaggcg gttcggcagg
     1261 tacaattgct agtccaagtc gagcaactta aatcctcgtt tttggtctgt tcggcttggc
     1321 gacattccaa aacccgccga atttaactaa gcaatggaaa aagggtggag attttttgag
     1381 tggttttttc aaaaaatact acccgtccat tgcttagtta aaagccaatt tttttatact
     1441 gtcgaacaaa acaccaaaac catggtacaa gataagagag gggatagttt ctagaaaaat
     1501 agtgtttaag ctagatatat caagggattt ggcgatttta ggcattacat gagggatgta
     1561 acgacattac atgagggatg taatcatatt acacgagaga tgtaattaac attacaatga
     1621 tttattgtaa cgttttgcat ttttactctt tacaatcatt acaacggttc gttataatga
     1681 cattacatta ttttatagta aggaggtgct attatggcta aagtgactaa tacaatttca
     1741 agaacagtcg ttaatgaaga tggtcaaatt actcatcaag aagaagagaa agttataaat
     1801 tggggtacag agccgaatta cataaaagtt tatttagaca caattttata tttaaaagac
     1861 cttcctaaag gattaaatag cattttatat gcttttctaa aaagaatgtc ttatggaaat
     1921 caattagttg ttaatgcggc attaaaaaga caaatagcaa aagaattagg actttctcta
     1981 tcaagtatta ataacgctat ttctaaattt gtgaaaggcg aattactaat tagggaagat
     2041 acaggacttt ataaagtaaa tcctcatttg tttggaaaag gcgattggaa agacattgca
     2101 aaaatacgtt tagaagttgt ttttgatcca aaaagaaaaa ctataatgtc tgaaattgaa
     2161 aaaaaagaaa ccaaagaaga aaaacaaaaa gtagaaatat aaaccccaaa aaaaatgttc
     2221 cctgcatact ggacacagag aacacttatg cagataagat tgagacccct atttgcatgt
     2281 ctccagtatg cacaaacctt ttttataaat caaatacatt tactggaggg aaatataatg
     2341 actgcaaacc aaatgttaga aatgattgtt gagcaaagtg tacaggaact agaaaaaatt
     2401 gaaaatctta aggaattaca taaacacgaa gacaaattca cttttgaaga atttgaacaa
     2461 cttgaagtag caatacttct agaaatgtcg aacatgaaag aagaatttta tagaaaaggc
     2521 tttaaacaag gcgtaaaatt cattttatct atgcaaaaat agagcaaaaa ggcgatgccc
     2581 cttacactgg gtatcgcctt ttttatagct ccatccctct atttttttgt gtctttggtc
     2641 gtctctcttt tttagattgc tctgattgac gttttaagcc cgatagagag tatttccgct
     2701 ctttccctag tattttatct aggaattctt ttaaacgctc tataaagccc tctaaacgct
     2761 tctgagggat tgtcttcgcc tctttattct cttccaggat agaaagccat tgttcgcctt
     2821 cttttcgatt tttaggctta tgcccattct ctagactatg gagaatcaaa cgtcctttct
     2881 tttcagtgtc gctaatctct aaatctttgt ttatatcttg caacatgctc ttatatacaa
     2941 tcgcatccgt tctgtgttcc ttcagcgtgt ctacttcttt ttccagtaaa ccaaattgaa
     3001 attccgcatt atctgccctt cgatccgcct cgtaatactt ctcttctaac tcttcaattt
     3061 tttcatttgc tgcagccatt cttttttcag cttgatattg tactttcatg ctagatacag
     3121 ataaatcttt taatttctca aattccttag acggcaatgt gaccttatct ccaaacatcg
     3181 ttttctttgc tgatgcttca attttcttca gtacttcttt tggttcttta aacactttca
     3241 tttctgcggt cgcatccgct ttttgttgtt gtttttgttt atgtatttca tcagaatgct
     3301 tttttaaatc cttaacggta tcaacaccaa tcgttttatc gtttaaaatg ccacctttgt
     3361 agatatgtgg cagttctttt tttaaaaatt catctaaatc ctgatgaaat gttcttaaat
     3421 cttttcttgt taaaacttct ttagctgaaa ccttttcacg ttgttttttt tcgtcccaaa
     3481 caacaggcat aaaagcaaag tgcatgtgtg gtcttgtttc gtcattatga acgttagcgg
     3541 acaacacatt tttttcaccc cc
//
