LOCUS       CP113372                3897 bp    DNA     circular ENV 13-DEC-2022
DEFINITION  MAG: Bacillus paranthracis isolate 4M plasmid unnamed8, complete
            sequence.
ACCESSION   CP113372
VERSION     CP113372.1
DBLINK      BioProject: PRJNA865942
            BioSample: SAMN30148851
KEYWORDS    ENV; Metagenome Assembled Genome; MAG.
SOURCE      Bacillus paranthracis (mixed culture metagenome)
  ORGANISM  Bacillus paranthracis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Identification and pathogenic (toxigenic) potential of
            Staphylococcus haemolyticus and Bacillus paranthracis consortium
            isolated from bovine mastitis in Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) Microbiology Laboratory, Federal Research
            Center for Animal Husbandry named after Academy Member L.K. Ernst,
            Dubrovitsy 60, Podolsk Municipal District, Moscow Region 142132,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/30/2022 12:02:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,200
            CDSs (total)                      :: 6,052
            Genes (coding)                    :: 5,834
            CDSs (with protein)               :: 5,834
            Genes (RNA)                       :: 148
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 91 of 218
            Pseudo Genes (incomplete)         :: 114 of 218
            Pseudo Genes (internal stop)      :: 62 of 218
            Pseudo Genes (multiple problems)  :: 38 of 218
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Bacillus paranthracis.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Bacillus paranthracis"
                     /mol_type="genomic DNA"
                     /isolate="4M"
                     /isolation_source="milk"
                     /host="bovine"
                     /db_xref="taxon:2026186"
                     /environmental_sample=""
                     /plasmid="unnamed8"
                     /geo_loc_name="Russia: Moscow region"
                     /collection_date="2020"
                     /metagenome_source="mixed culture metagenome"
                     /note="metagenomic"
     gene            complement(371..652)
                     /locus_tag="NRZ50_30955"
     CDS             complement(371..652)
                     /locus_tag="NRZ50_30955"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI30023.1"
                     /translation="MFFINKYSVILFSCLFVLISPLTAFAKAEMNIEQRLFINFENKVS
                     DLVITGMELLSNLDKMNGEISEEELYNLALNVGEEFSTRSKILNQISV"
                     /name="hypothetical protein"
     gene            1629..2123
                     /locus_tag="NRZ50_30960"
     CDS             1629..2123
                     /locus_tag="NRZ50_30960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WAI30024.1"
                     /translation="MEVENNTEKGDEVPKQRTENEIIYNGKSRWRNLDTGEIIETDEVI
                     KKTPRNGFMITYLTAIVQLIDSLGNKKMQVVKYILENMDKSTNTLIITTRELTEKSKVS
                     RQTVIDTLKTLEQAQIITRRTGAIMIHPSLVHRGKDTKEKYLLARFEDFSNNKAPINEV
                     E"
                     /name="replication/maintenance protein RepL"
     gene            2138..2422
                     /locus_tag="NRZ50_30965"
     CDS             2138..2422
                     /locus_tag="NRZ50_30965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WAI30021.1"
                     /translation="MKATGITRKVDELGRIVIPKELRKTLKIDIKDPIEIFTEEDSIIL
                     KKYESNMTCIITGEISSKNVSLFNGKIIVSPEGAELLAKELKKIITPVK"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            complement(2455..3771)
                     /locus_tag="NRZ50_30970"
     CDS             complement(2455..3771)
                     /locus_tag="NRZ50_30970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WAI30022.1"
                     /translation="MNKFAIIHMQKFQISDVQGIQKHNQRQGKSKSNLDIDYSKSEQNY
                     DLLNQQKIRYESTIKQEISERVKRKPRANSVVLSEFVVTASPDYMHSLSLEEQKRYFES
                     SLDFIQKRYGKQNTLYAMVHMDEATPHMHIGVMPITEDNRLSAKDIFTKKELVSLQQDF
                     PLEMREKGFDVERGEGSEKKHLSPQAFKEKQDLEVEVEHLSNVKTHLKTKVVETHNQLQ
                     QTTSYIEKQNETLQKIQQQFLSLDKKVKEKKQEFETFRNQIPDKPVSTSYLREETKTEV
                     TTKLFGKPEITEKKTGNIVVTREQWRDMTEKVNAAVIVKKDYERLQKTDLVKENKELHS
                     AVDEICDSLKESQKRNVGLQKENKQLSTEISSLKAHIRDLQTNIKVLYQQTKQVFKEQF
                     KTFRGLIKNELVGREVENHFEREHMREMKSRQKGYDMER"
                     /name="plasmid recombination protein"
     Typing          1667..2123
                     /name="rep_cluster_1950"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.323"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2459..3771)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.785"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agaccttgtt ggcttcgcca aatcacgcca cagcgtgata aagacaaggg gctttgcccc
       61 acaccccaca aggggaattc ttccccttga ccccatcaat cgaactcccc aacccctcaa
      121 tccttgaggg gcttcctcgc cggtcggcgt ccccaaaacg gttcgttttg aggaactgcc
      181 aagaggatgg agaatcggct gcacgtgtca attccttaat gctcctttca tggatcagca
      241 caaaccgctg aaccatttca gtccgcttac cattgacact taccgattat ttttcttctc
      301 cccataatcg tttggccagt tgttttaacc gctcgttaaa tttacaatat cctcttttat
      361 aatattaagt ctaaacacta atttggttca aaattttaga acgtgttgaa aattcctcgc
      421 ctacatttaa tgctaaatta tatagttctt cttcacttat ttcaccattc atcttatcta
      481 aattacttaa taattccatg ccagtaatca ccagatcaga aactttgttt tcaaaattta
      541 taaaaagacg ttgctctata ttcatttcag ctttagcaaa ggctgttagt ggggaaatca
      601 aaacaaataa acaactgaat aaaattacac tatatttatt tataaaaaac atgtctatct
      661 ccacctgagt ttttatttta tctaacctta tgatgttcta aagcaataat tttattttaa
      721 aaatcaaaaa gggcttattt gggtatatac ataaatttgt accctgcata cgttgataaa
      781 gtgcaaccac ccaactggta attcccacaa aacttttgaa aggacctatc atgaacgatg
      841 caatttgcaa atctttaaag acaatccttt gtaggaaaaa acatgaacat ttaagggcta
      901 cctgtagaga tccttgcaag ttcctgccac cataccctta gaataagtca cttttttaag
      961 tggcttattt tttataatgt attgtgataa actataatcg aaataaaaaa attttcctta
     1021 actaaatatt ttcaaacaca tatacatggt gtaagggagt cacactcctt attgaacatg
     1081 tataaaaaag gctaaaatcc tttcaccagg attttagcct ttttgtgttt ttccttgttt
     1141 ttgggtgtga gtgctggctg gggggttggg ggcctagccc ccaggaactt tacgtaacta
     1201 catagggagt gaaaatgttg cttagaacga tagaactggg tggttcggct ggtacaattg
     1261 ttggtccaag tcgagcaact taaccccgtt tttggtctgt tcggcttggc gaaagctaaa
     1321 tatcagccga atttcgaaat gcaatggagg aaaggtggag attttttgag cgtttttttc
     1381 aaaaaatact acctgaccat tgcatggaga aaaacccttt aaagaaaaaa caggatctat
     1441 atatttaagg cgatttttat cgccttaaat atatagataa gagtaacaaa gaggttaaaa
     1501 gtgaattttg agttgatata tagcttttga gacgtatttt gcttggtcta attttttata
     1561 ccttttggtc taatttttta taccttttgg tctaattttt tttgaccatt tggtctaaag
     1621 gtgataaaat ggaagtagaa aataatacag aaaaaggtga tgaagtgcca aaacaaagaa
     1681 ctgaaaatga aataatttat aacggtaaaa gtcgatggag aaacttagat acaggtgaaa
     1741 taatagaaac tgatgaagtc ataaagaaaa ctcctagaaa tggttttatg attacgtatt
     1801 taacagcaat tgttcaactg atagatagtt taggtaataa aaaaatgcaa gtagtaaaat
     1861 acatattaga aaacatggac aagtcaacaa atacattaat tataacgaca agagaattga
     1921 ctgaaaaaag caaggtatct agacaaactg taatagacac attaaaaaca ctagaacaag
     1981 cccaaatcat tactagaaga actggagcaa taatgattca cccaagcctt gttcatcgtg
     2041 gtaaagacac aaaagagaaa tacttacttg ctagatttga agattttagt aataataaag
     2101 caccaattaa tgaagttgaa taaaggagag aataaaaatg aaagctactg gtataactcg
     2161 taaagttgat gaattaggac gtatcgtaat cccaaaagaa ttacgtaaaa cactaaagat
     2221 tgatatcaaa gatccaatag aaattttcac agaggaagat agtattatac ttaaaaaata
     2281 tgaatctaac atgacttgta taataactgg agaaatttct agcaaaaatg tttctttatt
     2341 taacggaaaa attattgtaa gtcctgaagg cgctgaatta ttagcaaaag aattaaaaaa
     2401 aattattact ccagtcaaat aaaaacgccc tagaatatct ctaaggcgtt ttttctaacg
     2461 ctccatgtca tatcctttct gtctactttt catttctctc atatgttcac gttcaaaatg
     2521 attttctacc tctcttccaa ccaattcatt tttaataagc cctctaaacg tcttaaattg
     2581 ctctttaaag acttgtttcg tttgttgata caaaaccttt atgtttgtct gtaaatctct
     2641 tatatgagcc tttaatgagc ttatttctgt acttaattgt ttgttttcct tttgcaaccc
     2701 tacatttctc ttttgagatt ctttaagtga gtcacatatc tcatcgactg cactatgtaa
     2761 ttctttgttt tcttttacaa gatccgtttt ttgcaaacgc tcatagtcct tcttaactat
     2821 aaccgctgca tttactttct ctgtcatatc tctccattgc tcacgtgtta ccacgatgtt
     2881 tcctgttttt ttctctgtga tttctggttt tccaaacagt tttgttgtta cttccgtttt
     2941 ggtttcttct cgtaaataag acgttgacac tggcttgtca ggaatttggt ttcgaaacgt
     3001 ttcaaattcc tgtttctttt cttttacttt tttatctaaa ctcaaaaatt gttgctgaat
     3061 tttttgtaac gtttcattct gtttttcgat gtagcttgta gtttgttgta attgattatg
     3121 agtttctact acttttgtct tcaaatgcgt ttttacattg gataagtgtt ccacttctac
     3181 ttctaaatct tgtttttcct taaaggcttg aggcgataaa tgttttttct cagaaccttc
     3241 tccccgctct acatcaaaac ctttttctcg catttccaac ggaaagtcct gttgtaacga
     3301 taccagttct tttttagtga aaatatcttt agctgataaa cgattatctt cagtaattgg
     3361 cataacacca atatgcatat gtggtgttgc ttcatccata tgaaccatcg catacaacgt
     3421 attttgtttg ccataacgct tttgaataaa atctagtgaa ctctcaaaat aacgtttctg
     3481 ttcttctaga cttaatgaat gcatgtaatc aggcgaagct gttactacaa attcagaaag
     3541 tacaacagaa tttgcccgtg gctttcgttt taccctctca ctgatttctt gtttaattgt
     3601 actttcatat ctaatttttt gttgattcaa caaatcataa ttttgttcac ttttcgaata
     3661 atcaatgtcc aaatttgatt tactttttcc ttgtctctga ttatgttttt gaatcccttg
     3721 cacatcagaa atttgaaact tctgcatgtg aatgattgca aatttgttca tgtttttccc
     3781 tcacaattgt cttccgtagg aggactactt ttgagcctat cataacatat cgagagagcg
     3841 aatccaaagt tttggtatag cccactatac caaattacat ttggtgtggg ctctgcg
//
