LOCUS       CP113371                5235 bp    DNA     circular ENV 13-DEC-2022
DEFINITION  MAG: Bacillus paranthracis isolate 4M plasmid unnamed7, complete
            sequence.
ACCESSION   CP113371
VERSION     CP113371.1
DBLINK      BioProject: PRJNA865942
            BioSample: SAMN30148851
KEYWORDS    ENV; Metagenome Assembled Genome; MAG.
SOURCE      Bacillus paranthracis (mixed culture metagenome)
  ORGANISM  Bacillus paranthracis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Identification and pathogenic (toxigenic) potential of
            Staphylococcus haemolyticus and Bacillus paranthracis consortium
            isolated from bovine mastitis in Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Sokolov,S., Brovko,F., Solonin,A., Nikanova,D., Fursova,K.,
            Artem'eva,O., Kolodina,E., Sorokin,A., Shchannikova,M.,
            Dzhelyadin,T., Ermakov,A., Boziev,K. and Zinovieva,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2022) Microbiology Laboratory, Federal Research
            Center for Animal Husbandry named after Academy Member L.K. Ernst,
            Dubrovitsy 60, Podolsk Municipal District, Moscow Region 142132,
            Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/30/2022 12:02:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,200
            CDSs (total)                      :: 6,052
            Genes (coding)                    :: 5,834
            CDSs (with protein)               :: 5,834
            Genes (RNA)                       :: 148
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 218
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 91 of 218
            Pseudo Genes (incomplete)         :: 114 of 218
            Pseudo Genes (internal stop)      :: 62 of 218
            Pseudo Genes (multiple problems)  :: 38 of 218
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Bacillus paranthracis.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Bacillus paranthracis"
                     /mol_type="genomic DNA"
                     /isolate="4M"
                     /isolation_source="milk"
                     /host="bovine"
                     /db_xref="taxon:2026186"
                     /environmental_sample=""
                     /plasmid="unnamed7"
                     /geo_loc_name="Russia: Moscow region"
                     /collection_date="2020"
                     /metagenome_source="mixed culture metagenome"
                     /note="metagenomic"
     gene            650..1222
                     /locus_tag="NRZ50_30920"
     CDS             650..1222
                     /locus_tag="NRZ50_30920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017673839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WAI30020.1"
                     /translation="MHYNPYIYPYQYLYYVNVPIPMYNYGRQSVYRTFPNEIEHANRFD
                     SFRSSNNDRRISLTDYGPKPFVVNINEATKQNNTYRTALWTGTHLQVTLMSLNVGEDIG
                     LEVHSNVDQFLRIEQGQGIIQMGKNKDNLNFKRNVYDDYAIMIPAGTWHNLTNTGNIPL
                     KLYSIYAPPNHPFGTVHVTKADAMVNE"
                     /name="cupin domain-containing protein"
     gene            1412..2152
                     /locus_tag="NRZ50_30925"
     CDS             1412..2152
                     /locus_tag="NRZ50_30925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016083310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="WAI30018.1"
                     /translation="MKKVLEREEFVDIGGFQLYTKIFGSDKGTPTVVMDAGYGDYSKAW
                     NEIVSQIAEFTKVLVYDRAGLGKSDSSPNNRVSSEMVKELRTLLKKLQLTPPYIFVGHS
                     FGGVNVRLYSSLYPSEVAGLILVDSTPEDYRERFLPIMPSDFQEAYNKQFIYEGTYDQF
                     MKSLEEVRLHRKTLIDTNVIILSAGKKAFYSTDAQNLWLHMQKELLDLSTNSSLVIAPN
                     SGHYIQKDEPQYLINAVKKVLQIL"
                     /name="alpha/beta hydrolase"
     gene            2459..2605
                     /locus_tag="NRZ50_30930"
     CDS             2459..2605
                     /locus_tag="NRZ50_30930"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI30019.1"
                     /translation="MEQSGKGGDFLSVFLKKYYLFLVEVLALKEKKKKRPTKSWSFLKY
                     QRG"
                     /name="hypothetical protein"
     gene            2862..3425
                     /locus_tag="NRZ50_30935"
     CDS             2862..3425
                     /locus_tag="NRZ50_30935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WAI30014.1"
                     /translation="MEKHDENLEYHAETQTLVGQKKRELVDTETGEVIHVDQITKRVYG
                     TKNFWKMYLMDFLTVLGIIDNKQLDVFIYIAENTNQSNNLFIGTYRSISKDVGVGVSTV
                     TRIMKKLQESNFIKKKQNGVYIVNPNIMMKGNDTKRQILLSYYEEDKPLNSIDVLRGKQ
                     KALPEQETTKNESKKLGLETKNND"
                     /name="replication/maintenance protein RepL"
     gene            3418..3612
                     /locus_tag="NRZ50_30940"
     CDS             3418..3612
                     /locus_tag="NRZ50_30940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016132552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI30015.1"
                     /translation="MIEGVELNIYLDDDTISFSLSPVQTEVIFKALGLQFDAKTQTLSS
                     FSDNSLQKHVLPKINFVPK"
                     /name="hypothetical protein"
     gene            complement(3643..3828)
                     /locus_tag="NRZ50_30945"
     CDS             complement(3643..3828)
                     /locus_tag="NRZ50_30945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WAI30016.1"
                     /translation="MKKLRRNAIQCRKCGDVIESKFTHDFKYCSCGSVGVDGGLEYARI
                     IGDSENVIELHEYEDK"
                     /name="hypothetical protein"
     gene            complement(3843..5069)
                     /locus_tag="NRZ50_30950"
     CDS             complement(3843..5069)
                     /locus_tag="NRZ50_30950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WAI30017.1"
                     /translation="MSYAICRMQKFKMRDVKGIQIHNQREKESHTNHDIESERTHLNYD
                     LHNDGHIDYLKTVKDKIEQNVETNRAIRKDAVVMCEFVVTSDREFFDVLDMVDPAQQKV
                     FFKEAYSFLKDRYGERNIVHAAVHLDEKTPHMHVGMVPVTEENKLSAKQIFNRKELVSL
                     QDDFHAHMVEKGFYLERGVSSDKKHVEMRKFKAMTAKEEIKALETEIGQCLQKKEVVNK
                     QMEQLESRLNQLQNAVKSTNKVDKMEVKHKGGILRSKTVEMSVEDFEELKTKAKASEAF
                     KRENMTLHRKNEALQANNTDLHTTVKRLEKENEGLKPYKGKYERLAKLFDEMNKFYEKF
                     IPKEIPRFHEIIGFCKRKVNGSINRFSSLRYSEKALNENEKKGYESASKFLATEQKQQR
                     KERGNEREL"
                     /name="plasmid recombination protein"
     Typing          2863..3425
                     /name="rep_cluster_1909"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.823"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3898..5069)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.141"
                     /coverage="95.0"
                     /evalue="0.0"
ORIGIN
        1 aaacctcgga ctttgtccga acctacaagg ggaactcttc cccttgaccc cctcaatcga
       61 actccccaac ccctcaatcc ttgaggggct ccctcgccga ttggcaagaa caaacagtag
      121 tttgttcagt gccgagaggg ttcgggtata gtccagttgg tcaagtcaaa tactcccttc
      181 gcttcgctag gtccgttttg attgaccaac acacgcaacg acctctattg atcgtcttca
      241 ttttcctctc gtttttttca ccgcatattc atataatcgt tcattaaacc ctttatctat
      301 ctttaaaaat tgcttctggg gtaccgccag catttcggaa aaaatctacg ctaagaacat
      361 gcgagatttt atacagtttt ttggctaatc tagtaacaaa cgagaacccg aaaacccacg
      421 ccaggatagg aatgtataaa atgatgcata gaataataca aaaagaaaac atacaaaccc
      481 aattgtatca aaggtttgta cgttttcttt gctaccgata attgtgcgtt atgtatacta
      541 gatttgtata acgtattcat gtgtttttaa taagtcatct tgaatctaag tacattccct
      601 taacaagtgc atagaataat tttgtaaaaa cgcgaaagga ttgggaaaaa tgcactataa
      661 tccttatata tacccatatc aatatcttta ttatgttaat gtaccaatac caatgtataa
      721 ctatggaaga cagtctgttt accggacttt tcctaatgag atagagcatg caaacagatt
      781 tgattctttt cgctcttcta acaatgacag aagaatttca ttaacagatt atggaccaaa
      841 accatttgtc gttaatatca atgaagcaac gaagcaaaac aatacctatc gtactgcttt
      901 atggacagga acacatttac aagttacttt aatgagtctc aatgttggcg aagatatcgg
      961 tttggaagta cattctaacg ttgatcaatt cttacgtatt gaacaaggcc aggggattat
     1021 tcaaatgggc aagaataaag ataatttaaa ctttaaaaga aatgtctatg atgattatgc
     1081 cataatgata cccgctggaa catggcataa tctaaccaat acaggtaata ttcctctaaa
     1141 actttactcg atatatgctc ctcctaacca tccatttggt actgtacatg taacaaaagc
     1201 cgatgccatg gtaaatgaat aaaactatta aaaataataa aaaggatctt agattgaaga
     1261 tccttttttt atgccaataa taggacgtta tgttaacttc acatgcatat ggtattcaaa
     1321 atagatttct gattttcctt tttcatccat gatacaattt attaaataat ttacttgttg
     1381 taccttggaa tatggaagga gttaaatctg aatgaaaaag gttttagaac gtgaggaatt
     1441 tgttgatata ggtggttttc aattatatac aaaaatattt ggaagtgata aaggaactcc
     1501 aaccgtagta atggacgcag gttatggtga ttactctaaa gcttggaatg aaatagtatc
     1561 acaaattgcg gaattcacaa aagttctggt ctatgataga gctggtttag gaaagagtga
     1621 ttctagtccg aacaatagag ttagttctga aatggtaaaa gagctaagaa cattactaaa
     1681 aaaattacaa ttaacgcctc cttatatttt tgtggggcat tcctttggtg gagtaaatgt
     1741 tcgcttatac tcatctttat atccaagtga ggtagcaggg ctaatacttg tggattcaac
     1801 cccagaggac tacagagaac gatttcttcc catcatgcct tcagatttcc aagaagctta
     1861 taataaacaa tttatttatg aaggtacata cgatcagttt atgaagagtc tagaagaagt
     1921 acgattacac agaaaaacac ttatagatac aaatgttatt attctttctg caggaaaaaa
     1981 agctttttat tccactgatg cacaaaactt atggttgcat atgcaaaagg aattattgga
     2041 tttatcaact aatagttcct tagttatagc acctaacagt ggacactata tccaaaagga
     2101 tgagccacaa tatctaataa atgcggtcaa aaaggttctt caaatcttat aattaacttc
     2161 cgagttcgag aattatgtga acatgaaagt cccccagtca aacaatctgg aggacttttt
     2221 tatggtttgt tcttgtcttt tggtcttaca tttgcttttt ggctagagtg ctggctcgtc
     2281 gtgtgggggc gaaaccccac gtattacgta tctatttttt gaataaggac tattttggaa
     2341 cgaatgatct aagcggttcg gctcgtatac ttgcttgtcc aagtcgagaa ccattgcttt
     2401 gctttttgtc tgtcggcttg gcgagaaaag cacacgagcc gaaaaaactt ccgcaaggat
     2461 ggagcaaagc ggaaaaggtg gagatttttt gagtgttttt ctcaaaaaat actacctgtt
     2521 ccttgtagaa gttttagctc ttaaggaaaa aaagaaaaaa agaccaacta aaagttggtc
     2581 ttttttaaaa tatcaaaggg gctaaatagc ccctttgata ttagaagaga ggaaacgtgc
     2641 ataaaaagtg ggataaaccc agttatatca acggatttca cgtttttgct tgtgttcata
     2701 tttgatagca attgtgttca tttttgaaga tgtctgtatt ctttttgtga acacaattgt
     2761 attttttata taagtatgtt agtgttctta taaagagtta tttggtgttc aggtatgaat
     2821 acacattgaa tatatattaa atacatattg ggggatattt catggaaaaa catgacgaga
     2881 atttagaata tcatgcagaa actcaaacat tagttggtca aaagaaacga gaattagtag
     2941 acactgaaac tggagaagtt attcatgtag accaaataac caaaagagta tatggaacta
     3001 agaatttttg gaagatgtac ttaatggatt tcttaacagt tctaggaatc atagacaaca
     3061 aacaactaga tgtttttata tacatagcgg aaaatacaaa ccaatctaac aatcttttta
     3121 taggtacata tagaagtatc tcaaaagatg ttggtgtcgg tgtaagtaca gtgacacgta
     3181 ttatgaaaaa actacaagaa agtaatttta tcaagaaaaa acaaaatggc gtttatatcg
     3241 taaatcctaa catcatgatg aaagggaacg atacaaaacg acaaatatta ttaagttatt
     3301 acgaagaaga taagccttta aattctatcg acgtattaag agggaaacaa aaggctcttc
     3361 cagaacaaga aacaacaaaa aacgaatcaa aaaaattagg attggagacg aaaaacaatg
     3421 attgagggcg ttgaattaaa tatttattta gatgacgata caatatcatt tagtctatct
     3481 cctgtacaaa cagaagttat atttaaagca ttgggactac aatttgatgc aaagacacaa
     3541 acattaagtt catttagtga caacagttta caaaaacatg ttcttcctaa aattaatttt
     3601 gtacctaaat aaaaagcatt acatccattg atgtaatgct ttttatttat cctcatattc
     3661 atgaagttca atcacatttt ctgaatcacc aataatacga gcatattcta aaccaccatc
     3721 tactcccact gatccgcatg aacaatactt aaaatcatga gtgaatttag attcaattac
     3781 gtcaccacat tttcggcatt gaattgcatt tctccttagc tttttcattc ataatcctcc
     3841 tatcaaagtt ctcgttcatt tccacgttct tttctttgtt gcttttgttc agtcgcaaga
     3901 aactttgatg ctgattcata tccttttttc tcattttcat tcaatgcttt ttccgaatac
     3961 ctaagagaac taaatctatt tatagatcca tttacttttc tcttacaaaa accaattatt
     4021 tcatgaaatc tcggaatttc tttcggaata aacttctcat aaaatttatt catttcatca
     4081 aataattttg ctagacgttc atatttgccc ttatatggct ttaacccttc attttctttt
     4141 tctaaacgct tcactgttgt atgtaaatct gtattattcg cttgtaacgc ttcattctta
     4201 cgatgtaacg tcatattttc gcgtttaaag gcttctgatg cctttgcctt agttttgagt
     4261 tcctcaaagt cttctacgct catttcaaca gttttagagc gtaaaatacc acctttatgt
     4321 ttaacttcca tcttatcaac tttatttgtt gatttcactg cgttttgtaa ttgatttaaa
     4381 cgagattcta attgttccat ttgtttattt actacttctt ttttctgtag acattgtccg
     4441 atttcagttt ctagtgcttt tatctcttct tttgcggtca ttgccttgaa ttttcgcatt
     4501 tctacatgct ttttatcact tgatacacca cgttccaaat aaaagccctt ttctaccatg
     4561 tgagcatgaa aatcatcctg gagtgaaacc aattcttttc ggttaaaaat ttgtttggct
     4621 gataacttat tttcttccgt tactggaacc ataccaacgt gcatatgagg tgttttctca
     4681 tccaaatgta ccgctgcatg cacaatatta cgttcaccat atctatcttt taaaaaacta
     4741 tacgcttctt taaaaaatac tttttgttga gctggatcaa ccatatctaa tacatcaaaa
     4801 aattctctat cacttgtaac aacaaattcg cacatcacaa ccgcatcttt tcgaatcgct
     4861 cgattggtct ctacattctg ttcaattttg tctttcactg ttttcaaata atcaatgtgg
     4921 ccatcattat gtaaatcata atttagatgt gttcgctccg attcaatatc atgatttgtg
     4981 tgactctctt tttctcgctg attatgaatc tgaattccct tcacatctcg cattttaaat
     5041 ttctgcattc ggcaaattgc ataactcatt ttttcgcctc ttttccatag gttctacgtc
     5101 cttatcagtc cctagaacca tataaagtat atatacttct ctactgtttt tcggtggact
     5161 tccatgtaaa gtatagcaca ctatacttta ctatcgtaaa ggtgtgccct gcggggctat
     5221 tcggcaagcc gaatc
//
