LOCUS       CP110607                1952 bp    DNA     linear   BCT 12-NOV-2022
DEFINITION  Klebsiella pneumoniae strain INF281 plasmid pINF281.4, complete
            sequence.
ACCESSION   CP110607
VERSION     CP110607.1
DBLINK      BioProject: PRJNA646837
            BioSample: SAMEA3357277
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Foster-Nyarko,E., Cottingham,H., Wick,R., Judd,L.M., Lam,M.C.,
            Wyres,K.L., Tsang,K., David,S., Aanensen,D.M., Brisse,S. and
            Holt,K.E.
  TITLE     Nanopore-only assemblies for genomic surveillance of the global
            priority drug-resistant pathogen, Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2
  AUTHORS   Hawkey,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2020) THE UNIVERSITY OF MELBOURNE, 30 Flemington
            Rd, Australia
REFERENCE   3  (bases 1 to 1952)
  AUTHORS   Foster-Nyarko,E., Cottingham,H., Judd,L.M., Hawkey,J., Wick,R. and
            Holt,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2022) Department of Infectious Diseases, Monash
            University, Level 2, The Burnet Institute, Melbourne, VIC 3004,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 136x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 11/03/2022 17:22:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,115
            CDSs (total)                      :: 5,986
            Genes (coding)                    :: 5,832
            CDSs (with protein)               :: 5,832
            Genes (RNA)                       :: 129
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 154
            CDSs (without protein)            :: 154
            Pseudo Genes (ambiguous residues) :: 0 of 154
            Pseudo Genes (frameshifted)       :: 74 of 154
            Pseudo Genes (incomplete)         :: 83 of 154
            Pseudo Genes (internal stop)      :: 31 of 154
            Pseudo Genes (multiple problems)  :: 28 of 154
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="INF281"
                     /db_xref="taxon:573"
                     /plasmid="pINF281.4"
     gene            353..1429
                     /locus_tag="JMX78_30570"
     CDS             353..1429
                     /locus_tag="JMX78_30570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="UZJ18032.1"
                     /translation="MVDKVTEAAVVGGVDTHKDLHVAAVVDQNNKVLGTQYFSTTRQGY
                     RQMLAWMTSFGTLKRIGVECTGTYGSGLLRYFQNAGLEVLEVTAPDRMERRKRGKSDTI
                     DAECAAHAAFSGIRTVTPKTRDGMIESLRVLKTCRKTAISARRVALQIIHSNIISAPDE
                     LREQLRNMTRMQLIRTLGSWRPDASEYRNVTNVYRISLKSLARRYLELHDEIADLDVMI
                     AAIVDELAPELIKRNAIGYESASQLLITAGDNPQRLRSESGFAALCGVSPVPVSSGKTN
                     RYRLNRGGDRAANSALHIIAIGRLRTDAKTKEYVARRVAEGHTKMEAIRCLKRYISREV
                     YTLLRNQNRRINSIPITA"
                     /name="IS110 family transposase"
     gene            1446..1724
                     /locus_tag="JMX78_30575"
                     /pseudo=""
     CDS             1446..1724
                     /locus_tag="JMX78_30575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611389.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology. GO_process: GO:0015074 - DNA integration
                     [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
ORIGIN
        1 tggcactgga tgcgttgctg atggcggtct ggcggcgtaa gccttcagaa aacgtcattg
       61 tgcacagcga tagtaataat gccgatgtca gtctttacca ccacttagtt tgccgtttaa
      121 ccagattggt gtgattactg atgcagtgaa gaccttcccg catcctgact cacacagcga
      181 tcgacctttt gtgtcctgcc ctggacccgt cggttgccgg aagcgccttc atgcgaggca
      241 tctcctcacc gatgcgcgtg actcaagaag ggcctgacgg cttgtctcgt tactgtcctg
      301 tccgggttat ctgtctggag attcaactct gtttcctcac aggagctctg tcatggttga
      361 taaagttacc gaagctgccg ttgtgggtgg cgtggataca cataaagatt tgcacgttgc
      421 cgctgtcgta gatcagaaca ataaagtcct tgggactcag tatttctcca caacacgaca
      481 aggttaccgg cagatgctgg catggatgac ctcgtttggg acattaaagc gaattggtgt
      541 tgagtgcaca ggtacctatg gttccggttt gcttcgttat tttcagaacg ccgggttaga
      601 agttcttgag gtgactgctc cagaccggat ggagcgacgc aaacggggta aaagtgacac
      661 aattgatgct gaatgtgccg ctcatgcagc attctcaggc atcaggacag ttactcccaa
      721 aacgcgtgat ggcatgattg agtccctgcg ggtattaaaa acttgccgaa aaacagcaat
      781 atcagcccgc agagtcgctc tccagattat ccattcaaat attatctctg ccccggatga
      841 attacgtgaa cagctcagaa atatgacgcg catgcagctc atcaggaccc tgggttcctg
      901 gagacctgat gccagtgaat accgcaatgt aaccaacgtt tatcgcattt cattaaagtc
      961 ccttgcccga cgctatctcg agttacatga cgaaatcgct gatctggatg tcatgattgc
     1021 ggcaattgtc gacgagttgg cacctgagct gattaaacgt aatgctatcg gatacgaaag
     1081 cgcttcacag ttgctgatca ctgccggaga caatcctcaa cggttaagat cagaatcagg
     1141 atttgcggca ctgtgtggtg tcagcccggt ccccgtttct tctggaaaaa cgaaccgtta
     1201 ccgacttaat cgtggtggag atcgtgctgc aaatagtgca cttcacatca tcgccatcgg
     1261 acgcttacga actgacgcaa aaacaaagga atatgtcgcc aggcgtgtag ccgaaggaca
     1321 tacaaaaatg gaagcgatac gctgtctaaa gcgatatatc tcccgcgaag tttacacgtt
     1381 actgcgcaat caaaacaggc ggatcaacag tatcccgata acagcttgac tcttagaagg
     1441 gcgtccaggg tagtcagtat ggcagtgatg actggccgcg gttctgccgg gccaataacc
     1501 tggcaccggg catgagccgg cgtggcaact gccaggataa tgaggtggcc gaatcgttct
     1561 tcagttcact gaataaagag cgcatcagga agcggatata taaaacccgg gacatggccc
     1621 gggcggatat cttcgattac attgaagtgt tctacaaccg taaccgacgc catagtcacc
     1681 tcggtggcgt cagcccggag gccttcgaaa aggccccatc atgagaacag aatttgtcta
     1741 cgggcgtagg ggcagtccag gcagtgttaa tctggctgta accgcatcga tgccagatga
     1801 tcgcctgtac ggttatgcgc atttgttgac agaacgtatg ttactggttc attccccctg
     1861 gtgacgggaa cgctgggaac acagccttcg gctgccacac tcgtttcggt tccactgatt
     1921 acttatgacg aagaactgcc actgctcagg at
//
