LOCUS       CP110023                9316 bp    DNA     circular BCT 02-NOV-2022
DEFINITION  Enterococcus faecalis strain BE67 plasmid pBE67_2, complete
            sequence.
ACCESSION   CP110023
VERSION     CP110023.1
DBLINK      BioProject: PRJNA891504
            BioSample: SAMN31342475
KEYWORDS    .
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9316)
  AUTHORS   Gaeta,C., Pinzauti,D., Marruganti,C., Bollini,R., Fabbro,A.,
            Santoro,F., Grandini,S. and Pozzi,G.
  TITLE     The presence of Enterococcus faecalis in saliva as a risk factor for
            endodontic infection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9316)
  AUTHORS   Pinzauti,D. and Santoro,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2022) Dipartimento di Biotecnologie Mediche,
            University of Siena, viale Bracci 16, Siena 53100, Italy
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Flye, Medaka v. v. 2.8.3, v. 1.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 160x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/26/2022 12:50:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 76
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 146 of 175
            Pseudo Genes (incomplete)         :: 24 of 175
            Pseudo Genes (internal stop)      :: 21 of 175
            Pseudo Genes (multiple problems)  :: 14 of 175
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9316
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="BE67"
                     /host="Homo sapiens"
                     /db_xref="taxon:1351"
                     /plasmid="pBE67_2"
                     /geo_loc_name="Italy: Tuscany, Siena"
                     /collection_date="2021-04-21"
     gene            187..1155
                     /locus_tag="OLL91_14150"
     CDS             187..1155
                     /locus_tag="OLL91_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UYY05702.1"
                     /translation="MANELVKYHSELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKELSNYKATANMRFIDDLEETYKKILELRFGRRSQSGLTREFFVMFTKFKINGD
                     TDEPYVDIQIFEDALPLLNDLDSWVRYSLQQFNELQSSYSKTMFRLLKQFRTKGYAYFS
                     KEDFQELLDIPKSYKQGDIDRTVLQPIKQELTAIFKGLSIKKKYGRGRGKPVIGYQFTF
                     KPEIKNANDFNKHAPTKIEQSQALSQVNGQKNYEEQLTTLDDKIKEQERRLDLLKQLKE
                     LEQNKKDEKSLQIQTENKDRFTLSKRDKKDILSGLQNFNKL"
                     /name="replication initiation protein"
     gene            complement(2137..2316)
                     /locus_tag="OLL91_14155"
     CDS             complement(2137..2316)
                     /locus_tag="OLL91_14155"
                     /inference="COORDINATES: protein motif:HMM:NF038034.1"
                     /note="Among the bacteriocins in this family, lactococcin
                     G-beta was reported to be the beta chain of a two-chain
                     bacteriocin, while enterocin 1071B was reported to act on
                     its own; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin G-beta/enterocin 1071B family
                     bacteriocin"
                     /protein_id="UYY05703.1"
                     /translation="MKNIKNASNIKVIEDNELKAITGGGPGKWLPWLQPAYDFVTGLAK
                     GIGKEGNKNKWKNV"
                     /name="lactococcin G-beta/enterocin 1071B family
                     bacteriocin"
     gene            complement(2349..2522)
                     /locus_tag="OLL91_14160"
     CDS             complement(2349..2522)
                     /locus_tag="OLL91_14160"
                     /inference="COORDINATES: protein motif:HMM:NF019739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin 1071A family bacteriocin"
                     /protein_id="UYY05704.1"
                     /translation="MKQYKVLNEKEMKKTIGGESVFSKIGNAVGPAAYWILKGLGNMSD
                     VNQADRINRKKH"
                     /name="enterocin 1071A family bacteriocin"
     gene            2747..4841
                     /locus_tag="OLL91_14165"
                     /pseudo=""
     CDS             2747..4841
                     /locus_tag="OLL91_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292468.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]; GO_function: GO:0043214
                     - ABC-type bacteriocin transporter activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /name="peptide cleavage/export ABC transporter"
     gene            4860..6227
                     /locus_tag="OLL91_14170"
     CDS             4860..6227
                     /locus_tag="OLL91_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="UYY05705.1"
                     /translation="MQQKDWLDHSSVYSQQHHTFYRWVLYPVILFLCLGALFLAFAKKE
                     VVIRTPAQLTAQKTEKLQVPVEAKIKTNTLKENQTVKKGEELVTFETTPLQNEKKQLEQ
                     ENTTIEEQKKAAQTFIDSLTNEQNLFETEDSFGYSNQVKSFLAEKEATRYASKQSETNT
                     QKEQEAYNKTKEQLEKQLTTRQKEQKEWEQVRTAWINEQSLEGFSTEITSRYQAWQSQL
                     TDVPEEQKKQIKATTLATIDEQISQVKKEIEQLQSEQSKLVAPTTSDNEVSSQTEKTKQ
                     TKELALATAKQKVVELTETQKKNETALKTINEQLAQSTLKAPITGTVHVNEEVKNQLEV
                     PKGTTLAEIYPKSKDPQLTFTALIPTSESMRIKPGMAIHFKLDKKGVATKTIDGTLTEI
                     SENSTTTEQGAFYTIKGTLKPPKEFTGRYGLTGELSLIVGKKTYWQQIKDMLLNQE"
                     /name="bacteriocin secretion accessory protein"
     gene            6248..6556
                     /locus_tag="OLL91_14175"
     CDS             6248..6556
                     /locus_tag="OLL91_14175"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UYY05698.1"
                     /translation="MNELEKLQIQEQKLREKKKQLLAKEYSRLGRAFYKKAKVKSFDEA
                     QQAVTNYQPGTLGITNDQVEKLKQFANNLTWNESGNYWYTNEVKELTQFLAQFREEV"
                     /name="hypothetical protein"
     gene            7161..7544
                     /locus_tag="OLL91_14180"
     CDS             7161..7544
                     /locus_tag="OLL91_14180"
                     /inference="COORDINATES: protein motif:HMM:NF017524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UYY05699.1"
                     /translation="MTEEKEQGVNRKSPKKRHYTQVNFRLTEAEKERWQKQASSLNMTL
                     PKFSKHLINLFFDSGTIKQPKIDKQQGTEIARHLAKLGGNVNQIAKWCNTHQHDISDVQ
                     AKRLAENLAHVQKELKQVWQQLN"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7526..8566
                     /locus_tag="OLL91_14185"
     CDS             7526..8566
                     /locus_tag="OLL91_14185"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UYY05700.1"
                     /translation="MATIKLARTTSCNRAISYAEKRAVEKDGVNCEITNAKQEMATIRQ
                     LFSKTDKTQAHLCIQSFSPQESQQLGPKKINQLGIELAEKMAPNHQVAVYTHADTDHIH
                     NHLVINAVNLETGNKYSHHNDFQRVSALHDQLLQEHQLQIVEKQAVERRTMAERKLAEK
                     GQWVWKDQIRQTIDALMKDSSISSYKAFREGLDQRGIKLHERGQNVVYELLEGNKRVRG
                     AKLGTDYEKDVIKHELDHREQTRTIETQARTLANGLRTSDFSRGSDEITHDPRRDSPID
                     FRMGTAISDLKHQLRQATETPSERTERKNRERVEALKQTDTRPIRHTHQPEPDLTPRQS
                     GPSLGL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            8652..8966
                     /locus_tag="OLL91_14190"
     CDS             8652..8966
                     /locus_tag="OLL91_14190"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UYY05701.1"
                     /translation="MKELTANLEKAQEEVQEAMGMFARKQLAQAKTVNNIKDVAMSAVF
                     VLITLAVLWTVGYLLGTFFQLDEFYRWLVASTIGKGVLWGLGLALLVGSIGSVVYSVKN
                     "
                     /name="hypothetical protein"
     BGC             1..9316
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          188..1174
                     /name="rep_cluster_1070"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.595"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7527..8563
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcaaaggagt ttatcgtttt atgaacgtag ttttgtctgt ttatgaacgt agttttgtct
       61 gtttatgaac gtagttttgt ctgtttatga acgtagtttt gtctgtttag aagtttcttt
      121 ataaacgtat tacgttttac aaatacgttt caatatggta gtcttttctt caagaggtga
      181 ctatatatgg caaacgaact tgttaagtat cattcagaat taaataccat tcctttgagg
      241 aaatttactc ctgtagaaat gaacttgttc ttctctattg tttctagaat gcgtgaaaaa
      301 ggcgacaaga cagttcgttt tacttttgac caattaaaag aattaagcaa ttataaagcg
      361 actgctaata tgcgtttcat tgatgattta gaagaaacat ataaaaaaat tttagagttg
      421 cgattcggca ggagaagtca aagtggctta actagagaat tttttgtgat gtttactaaa
      481 ttcaaaatta acggtgatac tgacgaacct tatgtagata ttcaaatttt cgaagatgca
      541 cttcctctgt taaatgactt agattcttgg gttcgctact cattacaaca attcaatgaa
      601 ctacaaagta gttattctaa aaccatgttt cgtttattga aacaatttcg taccaaagga
      661 tatgcgtatt tctctaaaga agactttcaa gaattgttag atattccaaa aagctataaa
      721 caaggagata ttgatagaac tgttttacag cctattaaac aagaacttac tgctatcttc
      781 aaaggtttat caattaaaaa gaaatatgga agaggtcgcg gtaaacccgt tattggttat
      841 caattcacgt ttaaacccga gatcaaaaat gctaatgatt tcaataaaca tgcccctact
      901 aaaatcgaac agagtcaggc gttatctcag gtaaacgggc aaaaaaatta tgaagaacaa
      961 ctaacaactt tggacgataa gattaaagaa caagagcgta gattagactt attaaaacaa
     1021 ctcaaagaat tagagcaaaa taaaaaagac gagaaaagct tgcaaataca gaccgagaat
     1081 aaagatagat ttactctttc aaaaagggat aaaaaagata tacttagcgg tttacaaaat
     1141 ttcaataaat tatagtaaga agcacatagt ttagcagcaa tataaatata ttaaaccaac
     1201 tatatcaaat atctattgaa taccttaaat aaatttattt tcaatacttt acttttggag
     1261 ggattgctat gttattttta ctaaaattta ttacgctatt aagcattatt tcttatttat
     1321 atctatgtat tatgtatatg aaaaatatgg acaaatataa attattagca ttaatactta
     1381 tagggggctg cattttatta tctatcgttc taagtatatt taacttatcg tttatcttaa
     1441 ttagactgtc tctattactt ggattatctg gccttttact attgcttaaa ccagccatta
     1501 aaaatacatt taaagatgat ttttcatttt tagaaggtat agcattaagt gctataacat
     1561 caagttactt agtaagctta atttcattat ttatacggtc ttaattatta ttgtgcgttt
     1621 ttctaaaagg taatacaaat ataataaacc tgtggtgcta gttgattttt cttaaaatca
     1681 actagcacca caggtttatt attacgttaa aggaaagagc ccataatata ttaatttact
     1741 taattaaata gttcagtaca atgtatatac attgagtaaa aaagaataac acaaacattg
     1801 tttgattttt ttttgttaaa actgatttct ttttttaaaa ttatatataa atatataata
     1861 gtaattatgc tcaacaattc taacaaatta gttaacagac ttaaattgaa aggtactatc
     1921 atagtaaaaa ccattagaat gaatacgcta aagaaaaatg tataagcttt ttttattgca
     1981 tgatctgaat aattgatatc aattccaatt aaaatgtata aaaatgttat aaaattaaaa
     2041 aaggttatga tgcttataaa aatatccata tttatctcct taaaaataat aaaaacatat
     2101 ggcgttaacg ccatatgttt ttattatact ctttaattat acattcttcc acttattttt
     2161 atttccttct tttccaattc cttttgcaag acctgtaaca aaatcatatg cgggttgtag
     2221 ccatggtaac cattttccag gtcctccacc agtaattgct ttcaactcat tatcttcaat
     2281 tacttttata ttacttgcat tcttaatgtt tttcatatta gcatatctcc tctatttgat
     2341 ttcgattctt aatgtttctt tctattaatt ctatcagctt ggtttacatc actcatatta
     2401 cctaatcctt ttaaaatcca ataagcagct ggacctacag cattacctat tttactaaaa
     2461 accgactctc ccccaatagt ttttttcatt tctttttcat tcaatacttt atattgcttc
     2521 atttatcgtt ccccctaatt atttttgtaa tataattctg gaattatatt acttaattta
     2581 ttaaatcaaa gtctttttat tatgtcaatt aattatataa aaaggttttc aaaaatattt
     2641 tattatagta atattgataa atgaataata acacaagtga ggagaaactt tgaaaaagat
     2701 tatttaccaa caagacgaaa aagattgtgg cgtagcttgt attgccatga ttttaaagca
     2761 ttataataca gaaataccca ttcacaagtt acgtgagttg tctggaacag atttagaggg
     2821 aacttccgct tttggtctaa aaagaacttt agaaaaaatt ggttttgatt ctcctgctat
     2881 tcaagctgat aatgaagtat ggaaagaaaa agaattaccg ttacctttaa ttgcgcatgt
     2941 cttaattgac aataaataca tgcactatgt cgtggtttat ggaataaaag gagatacctt
     3001 attaattgct gatccagatc caacagtagg aaaagtaaaa aaatcaatgg aagaattttc
     3061 caaagaatgg acaggcgttt tactgttacc tacacctaaa gagtcttata ctcctaccaa
     3121 agaaaaagtc gctggattga gctctttttt acccattatt tggcgtcaaa aaggcttagt
     3181 ttttcacatt attttagcgt ctctttttat tacgctattt gggattggga gctcctacta
     3241 ttttcaagga attttagatt actttattcc taatcaagca cgttctactt taaacattgt
     3301 ttcgattggc ttgattgctg tctatctgtt ccgtgtcctg tttgaataca gtcggagtta
     3361 tctcttagtt atcctaggac aacgcatgag catggccgta atgcttcaat actttaaaca
     3421 tgttcttact ttacctatga acttttttgc tacccgtaaa tcaggggaga tcatttctcg
     3481 ttttctagac gcaaataaaa tcattgatgc cctcgccagt gcaaccttat ccgtcttttt
     3541 agacattggg atggtcttct tagtagggat taccttagcc attcaaaatg gtacattatt
     3601 cttaattacc ttagcttcgt taccgtttta ttttgtggcc attctagcct ttgtcaaaag
     3661 ttatgaaaaa gccaatcaag aagaaatggc ggctggcgct accttaaatt caagtattat
     3721 cgagagccta aaaggaatcg agacgatcaa ggcctacagt ggagaagaaa aagtctataa
     3781 tcgcgtggat cgagaattta ttcgattaat gaaaaagtcc ttccggactg ttacgctaga
     3841 taatgtgcaa caagggatta aacatgctat tcagttaatc agtagtgccc ttattttgtg
     3901 gctaggttct ttctatgtca tggatggtac gattagcctt ggccaattga ttacttacaa
     3961 tgccttgttg gtgtttttca cggatccttt acagaatatt attaatctac aagtcaaaat
     4021 gcaaacagcg caagtggcca acaaacgctt aaacgagatt tttgccattg aaccagaaca
     4081 aacaaacgat tcttcggaac gcgaaattcc aaatcaaacg ttccaacaag aaataagact
     4141 agaaaatgtc tccttctcct ataacatgaa agcacctacc ttaaacaata tttcttgcac
     4201 cattgcaccg aaaagtaaaa tcgccttggt tggggtaagt ggttcaggga aatcaacatt
     4261 agcaaaatta ttagtgaact tttatgcgcc ttcagaagga accattcgct acggaaaaat
     4321 gaatcattta gatattccgt ttcaacaatt acgtgaacac gtcacctatg tgccgcaaga
     4381 gtcttttttt ctttagcggg acaattgtag aaaacctaac gtttggctta cagaacatgc
     4441 ctagttttga acggattatt gaagtgtgtg aagccgttca actaaacaca ttcattgaac
     4501 aacagccttt acgctatgac atggtactag aagaaggagg ggcgaatctt tcaggaggac
     4561 aacggcaacg attggcgatt gctcgtgctc ttttgaaaga cgcggatgtt ttaatcttag
     4621 acgaagcaac tagcggatta gacacactat tagaacatgc gattcttgat tatttactaa
     4681 cgttagaaga gaaaacattg ctttttattg cgcaccattt gcccattgct aaagcgtgcg
     4741 atcacattct agttcttcat gaagggcaac tggttgaaca aggggctcat gacgacctcc
     4801 gttataatca gggggtttat caacagttat gggatattta ggaaaggaca gaaaaattga
     4861 tgcaacaaaa ggattggctc gatcattcga gtgtttatag ccaacagcac cacaccttct
     4921 atcgatgggt cttgtatcct gtcattcttt ttttgtgctt aggggcctta tttttagctt
     4981 ttgcgaaaaa agaagtcgtg attcgaacac ctgcacaact aacggcacaa aagacagaaa
     5041 aactacaagt accggtagaa gcaaagataa aaacaaatac tctgaaagaa aaccaaacag
     5101 taaaaaaggg cgaagagtta gtcacttttg aaacaacccc tttgcaaaac gaaaaaaaac
     5161 aattagagca agaaaacaca acgattgagg aacaaaaaaa agcggcacaa acttttattg
     5221 acagcctaac aaatgaacaa aatttatttg agacagaaga ttccttcggc tatagcaatc
     5281 aagtaaagag ttttctggct gaaaaagagg caacgcgcta tgcctcaaag caaagtgaaa
     5341 caaacacaca aaaggagcaa gaagcgtaca acaaaaccaa agaacaacta gaaaaacaac
     5401 taactacgcg tcaaaaagaa caaaaggaat gggagcaggt acgcacggca tggatcaatg
     5461 aacaatccct agagggattt tcaactgaaa tcacatccag gtatcaggcg tggcaatctc
     5521 aattaaccga cgtaccagaa gaacaaaaga aacaaattaa agcaacgaca ttggctacaa
     5581 tcgacgaaca aatcagtcag gtaaaaaaag aaatcgagca acttcaaagc gaacaatcaa
     5641 agctagtagc gccaaccacg tctgataatg aagtaagtag tcaaacagaa aaaacaaagc
     5701 aaaccaaaga actagccttg gcaacagcga aacaaaaagt ggtagaatta accgagaccc
     5761 aaaagaaaaa tgagacagca ctgaaaacaa tcaacgaaca actcgcacaa agtacgttga
     5821 aagcacctat cacaggaact gttcacgtaa atgaggaagt caaaaaccaa ttggaagtcc
     5881 cgaaaggcac aacgttggca gaaatttatc cgaaatcaaa agacccacaa cttacgttta
     5941 ccgcacttat tcctacgagt gaaagcatga gaatcaaacc tggaatggca atacatttca
     6001 aactggataa gaaaggagta gcaactaaaa caatcgacgg aacgctaacg gaaatttcag
     6061 aaaacagtac gacaactgaa caaggagctt tttatacaat aaaaggcaca ctaaaacctc
     6121 caaaagagtt taccggtcgc tatggtctta caggggagct ttctctcatt gttggaaaga
     6181 aaacctattg gcaacaaatc aaagatatgt tattaaatca agaatagaaa aagagtgagg
     6241 gattatcatg aatgagttag aaaaactaca aatccaagaa caaaaactac gagaaaagaa
     6301 aaagcaacta ctcgcaaagg aatacagccg tttaggacgt gccttttaca aaaaagcgaa
     6361 agtgaaatcc tttgacgaag cccaacaagc cgtcaccaat tatcaaccag gaacactcgg
     6421 cataacaaac gatcaagtag aaaaattaaa acagtttgct aataacctaa cctggaatga
     6481 atcaggcaac tattggtata cgaatgaggt caaagaatta acacaatttt tggcacaatt
     6541 ccgcgaggaa gtgtaagtcg attgaacggg tttagttgct tcgataatcc gaagcaactt
     6601 ttcctccatt tatggaggaa aaaaggggcg ttttcgaaca gaaaaccgac cctgaaaacg
     6661 ttggagaaaa atcgccgtac aggcgacctt tttcaagcaa cgttcggggg attgggggat
     6721 acccccaaca agctatttgt agtacgaagt gctagcaaat aaactgcttg ccaaaacctc
     6781 aggatgggca cataatgagg ttgcctgttt tgttggtaag tggtgactga ctaaggaaca
     6841 acgaacctac aaaacttaga ggaaaggtgt aaccgaatag ggcacaactt gacgttttct
     6901 cttgtgagag ttgtgagtga caaacgaaca attggcacta gagaaaggct aaagaaaatt
     6961 gaccgaatag ggaatttttc tggcgcgatc ttttgatttt tttttcgtag ccgtaggcac
     7021 gaaaacagac aaagggaaac gactgaagaa ggagttttct ctgtctgctt ggggtgagat
     7081 tgtcgaacag acaactcgca acccaagtga cggaacaaat cggcgaacag ccgactttga
     7141 gaaggaaagg agcgaacaca atgacagaag aaaaagagca aggagtgaac cggaaatccc
     7201 ccaaaaagcg ccactatacg caagtgaatt ttcgtttaac tgaagccgag aaagaacgct
     7261 ggcaaaaaca agccagtagc ctgaacatga ccttaccaaa gttctcaaaa cacttgatta
     7321 atttattctt tgattctggg acaatcaagc aaccaaaaat tgacaaacaa caaggaacgg
     7381 aaattgcccg ccacttagcc aaactaggcg gaaatgtgaa tcaaatcgcc aagtggtgca
     7441 atacccatca acacgacatt tcagacgttc aggctaaacg gttagccgag aacttagcac
     7501 acgtgcaaaa ggagttgaaa caggtatggc aacaattaaa ctagcgcgta ccaccagttg
     7561 caatcgggcc attagctatg cggaaaagcg cgccgtggaa aaagacggcg tcaactgcga
     7621 aatcaccaac gccaagcaag aaatggccac gattcgccaa ctcttctcaa aaaccgataa
     7681 aacacaagcc catttgtgta tccaatcctt tagcccacaa gagagccaac aacttgggcc
     7741 aaagaaaatc aatcagttag gcatagaatt ggccgagaaa atggccccca accatcaagt
     7801 agcggtctat acccacgccg acacagacca tatccataac catctggtga tcaatgcggt
     7861 gaatttagaa accggcaaca aatacagcca ccataacgac tttcagcgcg tatctgccct
     7921 ccatgaccag ctattgcagg aacaccagtt acagatcgtc gaaaaacaag cggtggaacg
     7981 tcggaccatg gccgaacgaa aactcgccga aaaagggcaa tgggtttgga aagaccaaat
     8041 caggcagaca atcgacgcct tgatgaagga tagctctata agctcatata aggcgtttag
     8101 agagggtttg gatcaaagag gtataaaatt acatgaacga ggtcaaaacg tcgtatacga
     8161 gcttttagag gggaataaga gggttcgcgg agcgaagcta ggaacagatt atgaaaagga
     8221 cgtgattaaa catgagttgg accaccgaga acaaacacga acaattgaaa cacaagcaag
     8281 aacgcttgca aatggactta gaacgagcga ttttagtcga ggaagtgacg aaattacgca
     8341 cgatccaaga agagattccc caattgattt cagaatggga acagcaattt cagaccttaa
     8401 gcaccaactt cgacaagcaa cagaaacacc atcagaacga actgagcgaa aaaatcgaga
     8461 acgagttgaa gcacttaaac agacggatac aagaccaatt agacacactc atcagccaga
     8521 accagatctt acaccacgac aatcaggtcc ttctctcgga ctatgaaaac tacctggaca
     8581 aacatgtcaa agaaatcaat caagaatacc gcctacttgt cgaacaccaa gcccttgcac
     8641 aggtgagcca tttgaaagaa ctgacagcaa acctggaaaa agcacaagaa gaagtgcaag
     8701 aagcgatggg tatgttcgct agaaaacagt tagcccaggc aaaaacagtg aataacatca
     8761 aagacgtggc catgagtgcc gtttttgtct tgattacact cgctgttctt tggacagtgg
     8821 gctatctatt aggcacgttt ttccagttgg acgaatttta tcggtggtta gttgcctcaa
     8881 ccattggcaa aggcgttctc tgggggctag ggcttgcact cctagttgga tcaattggta
     8941 gtgtggttta ttcggtaaag aactaatggt ctttccaggg tcgttagggc ttgccctgac
     9001 cgtctgaaag acgcaaacaa ggaaaactat caaggaatgc ttaactcgtc ttagaaaggc
     9061 tgacagtggc agttaggacg atcccacagg tctccttgtt gtgggtttgc ttttagcgag
     9121 tgaccgaaag ggaacgctgc aagtacaatg tgggagcaaa gcgagcactc cttttagcga
     9181 acaacgtgag cgaagcattt ccagtttatt gattacgcga accagttttt agaaaaaaga
     9241 ggtctttttt aggcgggaat ttttgccccc ctcttttata aactcttttt aaacctcttt
     9301 taaacctctt taccgt
//
