LOCUS       CP110021                5128 bp    DNA     circular BCT 02-NOV-2022
DEFINITION  Enterococcus faecalis strain BE67 plasmid pBE67_1, complete
            sequence.
ACCESSION   CP110021
VERSION     CP110021.1
DBLINK      BioProject: PRJNA891504
            BioSample: SAMN31342475
KEYWORDS    .
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Gaeta,C., Pinzauti,D., Marruganti,C., Bollini,R., Fabbro,A.,
            Santoro,F., Grandini,S. and Pozzi,G.
  TITLE     The presence of Enterococcus faecalis in saliva as a risk factor for
            endodontic infection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Pinzauti,D. and Santoro,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2022) Dipartimento di Biotecnologie Mediche,
            University of Siena, viale Bracci 16, Siena 53100, Italy
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Flye, Medaka v. v. 2.8.3, v. 1.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 160x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/26/2022 12:50:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,919
            CDSs (total)                      :: 2,843
            Genes (coding)                    :: 2,668
            CDSs (with protein)               :: 2,668
            Genes (RNA)                       :: 76
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 146 of 175
            Pseudo Genes (incomplete)         :: 24 of 175
            Pseudo Genes (internal stop)      :: 21 of 175
            Pseudo Genes (multiple problems)  :: 14 of 175
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="BE67"
                     /host="Homo sapiens"
                     /db_xref="taxon:1351"
                     /plasmid="pBE67_1"
                     /geo_loc_name="Italy: Tuscany, Siena"
                     /collection_date="2021-04-21"
     gene            complement(760..1056)
                     /locus_tag="OLL91_00005"
     CDS             complement(760..1056)
                     /locus_tag="OLL91_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UYY03118.1"
                     /translation="MGLMTGYNMTKEEYEVTQAVLERLDKFESELEALLLDLEPLDLSR
                     LDTVVRDETLRMFNYLSRSYILLIDTVAYKVTLEEDISCGIVKIDGQSLDEEE"
                     /name="hypothetical protein"
     gene            complement(1183..1905)
                     /locus_tag="OLL91_00010"
     CDS             complement(1183..1905)
                     /locus_tag="OLL91_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UYY03119.1"
                     /translation="MSDKSELAVKYQNELNLVPLKNFNAKQMDLFFALCARMKDKGVEN
                     IRFSFEELKELSDYKMTATKAFVADLEKLYKDMLNLSYRTENDDEIEYFVLFNGFKIDK
                     KQKFVEVRVNQDLDYIINGLTTEFSRFELSAFTSIRSTYAKTLFRLLMQYRSTGYYVVS
                     IEDFRELLDIPEYYQMGNIDQKVLKPAMKELHNYFENLEVTKIKAKKGNKIAKLEFTFT
                     GLKTNKPSVTMHDWVNGE"
                     /name="replication initiation protein"
     gene            2553..2639
                     /locus_tag="OLL91_00015"
     CDS             2553..2639
                     /locus_tag="OLL91_00015"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="UYY03120.1"
                     /translation="MIESLISLIIAPLFVGLVLLLVDRWLDD"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(3158..3358)
                     /locus_tag="OLL91_00020"
     CDS             complement(3158..3358)
                     /locus_tag="OLL91_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UYY03116.1"
                     /translation="MKKLLVLILGVSFISVALSSTTEASAREWDSIECQPGFYWKKGGW
                     TAGGYHKAGGFCIPMHTRWTK"
                     /name="hypothetical protein"
     gene            complement(3447..5126)
                     /locus_tag="OLL91_00025"
     CDS             complement(3447..5126)
                     /locus_tag="OLL91_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="UYY03117.1"
                     /translation="MSKIVARMEKMKDGNLSGIQRHNQRETNNHSNPDIDIEKSHLNYD
                     LVNPGSINYREKIKQIIESQRISKRAVRKDAVLVNEWIITSDTAFFQENTDTQAFFTDV
                     VAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYEGRLSSKQVFSRQNLLEIQEELPT
                     YLQERGYAIERGLRGSPQKHLSVKEYKDVQKELQVSKQELANQEQILAEKERELHSYEQ
                     AIFRIDRLLEEKNEEVKGKVQAMNQLETKRNEIINSLQTTVLPDLANVKEKKFSLNGLK
                     YVLSASDFSAIKSITKDFETLRTQNKHLKIENNNLAQKNKELTQVNHEQELQITELAYE
                     LTLKKEQLTAFEEKQLNYDQSEQIKQELDTTTQTVSVLETENKRLKDKITDLTYDFSEL
                     NEKYQEIKLKFDGLTTYLHERLGHAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTY
                     LIDEKGTLFLPPDTGSKGVTMSLNPHFYPLKAPFYNRETGCYEFVGEYMANGRSRTNTL
                     QLSEEQVLEKKAFTFEQSTDKKAWQQSKKYSRDMSKGRGLSR"
                     /name="plasmid recombination protein"
     Typing          269..528
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="80.0"
                     /coverage="81.0"
                     /evalue="1.95e-45"
     Typing          complement(1184..1905)
                     /name="rep_cluster_2301"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3451..5126)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="95.0"
                     /evalue="0.0"
ORIGIN
        1 ttttcttgtg tcgttcggtg tctttggatt tcggcagtac ttccaagtaa agtataaacg
       61 cactatactt tacttcgtaa aggtgcgctc tgccgagggc tgtcggctag tactcgaaag
      121 gtcagccttt aggcttccca atctcgcact tagccgattg cctacggtca aactttccat
      181 ttctgctaag gcagaaactc caaatttccc tccgtcagaa aaccgtttaa ataacgactg
      241 cctgaaatta gtgaacgtgc tagctttctg ctagccggtg ctagtaatgt gctaatttgg
      301 tgctaaaaac ctgctagtca ctgctaggaa tctgctagtt tgggtgctag aaacctgcta
      361 ttgagtgcta aaaatgtgct agtttacctg aaagtacctg ctagtaaagt gctagcgaag
      421 aacgaaagtg taccccacgg gaaagccttg cactgcaagg cggggtacct tgtgtgcccc
      481 ccagttcccc ttatgctctt ccgaaacaca ggaagaaatt aagcgaattt caacagtaaa
      541 agcaaaaaat aacagcagtg ggattggggg aaccccaaaa atttgaacga ctcctaccag
      601 tatttttggt aggagtcgtt caaaatatcg caacatggta ccatgttgct ccgcttaaaa
      661 aaagaaagcc taccccaaaa tggaagtagg ctaatcttga tatttttcga gtgttatccc
      721 aaatagtcaa cattttttta taacaagcaa gggtagtttt tattcttctt cgtctagaga
      781 ttgaccgtca atcttcacaa tcccacagga aatatcttct tctagcgtaa ctttgtaggc
      841 taccgtatca ataagtagaa tgtatgaccg acttagataa ttaaacattc ttagcgtttc
      901 gtctcgaact acagtatcta atcgtgataa gtcaagtggc tccaaatcaa gcaatagcgc
      961 ttctagttca ctctcaaact tatctagtcg ctccagtaca gcctgtgtga cttcgtactc
     1021 ctcttttgtc atgttgtatc ctgtcattaa tcccataagt aggacctcca aaataattta
     1081 ttttagaagt cctcacgcaa atatgctaaa ataatctagc agtccggaag attgcgtgag
     1141 gaacttgcta acttgcttaa tgtgaggggg caagttcttt ttttattctc cgtttaccca
     1201 atcgtgcatg gttacgcttg gtttgttggt tttcagacca gtaaacgtaa attctagttt
     1261 ggcaatctta ttgccttttt tggctttgat ttttgttact tctaaattct caaaatagtt
     1321 atggagttct ttcattgctg gttttaaaac cttttggtca atgttaccca tttgataata
     1381 ttcgggtatg tctaacaatt ctctgaagtc ttcaatactg actacataat accctgttga
     1441 acgatattgc attaacaaac gaaacaatgt tttagcatat gttgagcgta ttgaagtaaa
     1501 agcggacagc tcaaacctac taaactctgt tgttaaacca tttattatat agtctaaatc
     1561 ttgattaact cgaacttcaa caaacttttg ttttttgtcg attttaaatc cattgaataa
     1621 aacaaaatac tctatttcgt catcgttttc tgtacgataa gaaaggttca gcatatcttt
     1681 atacaacttt tctaagtctg ctacaaaggc ttttgtagca gtcattttgt agtcactcaa
     1741 ttcctttaat tcctcgaagg agaaacgaat attctctact cctttatctt tcattcgtgc
     1801 gcataacgca aagaacagat ccatttgttt tgcattaaag tttttaagtg gcacaagatt
     1861 taactcattt tgatatttaa cagctaattc cgatttatca ctcattcctt tcgccccctc
     1921 acaattcgaa tattaaaaca aacaaccaac tttgtcaaca gttggttgtt tgttttaata
     1981 ttatgaaaat aaaacagata aaaagttgtt ggtagtcaga taaaaagttg ttggtagtca
     2041 gataaaaagt tgttggtagt cagataaaaa gttgttggta gtcagataaa aagttgttgg
     2101 taccatctgc tcttccttag agccacgtgg cacacagccc gcctaaaaag gttttaaaaa
     2161 tatatataaa atatatataa aagaaacgcg tgcgcgcgcg gaaatttccc gcccaaaatt
     2221 aacctctttt tttctacaaa caggtccgcg atatttacaa aactggcact aaaaaatcaa
     2281 aaaaggagtg agcgaaaatc ccgctcacat tttgcttgca gcgttcactt cgttcactcg
     2341 ctaaaatcaa aatcaatacc gttgacaagg taaatcattt ggttaggctc tatttgcttg
     2401 tctgaagatc tttaagacat gtttagttgt tttagggtat cttggttagg attgcgcctt
     2461 gcaggcggtc agggcaagcc ctaacaaccc ccacaagaaa ttcttcttgt gatatacttg
     2521 ttttggcaac tgccccgaaa gggggtgtaa acatgattga aagcttaatt tcgttgatta
     2581 tcgccccact ttttgtgggt ttagtacttt tgctagttga tcgttggcta gacgattagc
     2641 agataatcta tgcagttgct tttctagctc caacgctgaa atcgcaacag tgtaaaaaag
     2701 ccctcctact gagaatagga gggctttttg gtgtaaacat gattttgctt aatttcgctt
     2761 cgcacttaca caataacatt ttttttctgt attttcaaac atcagattag attacgcaaa
     2821 acgtcaatta gagaaatacc atatcgaaat agttagtatt aaacatagta atactatgag
     2881 gatagacaat attttacttt ttggactatc ttcaaagctt ttattttctg gtctgtaagt
     2941 gggattcact actttcgcgt tgtaatccat tgtacttccc aaatatactt ctgaaggaat
     3001 taaaaaaaat aataaaattc cgtaaaaaat aattatcaat atgggcaaat caatattaac
     3061 taaaaaagaa gttatatagc ttaaaactaa aggaatagta atataaaata tattttttat
     3121 tagatatttt ttcatgaaat aacctcaaat ttagcaacta ttttgtccat cttgtatgca
     3181 ttgggataca aaatccaccg gctttatgat atcctcctgc agtccacccg ccttttttcc
     3241 aataaaatcc tggctgacac tctatagaat cccactctct agcacttgct tctgtagttg
     3301 aacttaaagc aacagagata aaagacaccc ctaaaattag aactaacaac tttttcataa
     3361 tgcttacctc actttttttt aatttttatt aatatatttt aatttaactg tttttatgtg
     3421 tcaataattt atcaattaaa ttatttttaa cgagataaac ctcgtccttt tgacatatct
     3481 cttgaatatt ttttactctg ttgccaagcc tttttatctg tcgactgttc aaacgtaaaa
     3541 gctttcttct ctagcacttg ttcttcagac agttgtaaag tattcgttcg agaacgtcca
     3601 ttagccatat attcgccgac aaactcataa caccctgttt ctctgttgta aaacggggct
     3661 tttagggggt agaaatgtgg gtttaaggac attgttaccc cttttgaccc cgtatctggt
     3721 ggtaaaaaca atgtcccttt ttcgtcaatg aggtaagttc tatccttgga atatcgctct
     3781 agggattttt ttaatttagg cgcgttctga aggtacttca ctccttgctg aatcgtaaat
     3841 aaaatggttt ctttagcatg acctagtctt tcgtgaaggt aagtagttaa cccgtcaaac
     3901 tttaatttta tttcttgata tttttcattt agttcagaaa agtcgtaggt taagtcagta
     3961 attttatctt ttaaccgctt attctctgtt tctaacacag aaactgtttg tgttgttgta
     4021 tcaagttctt gtttgatttg ttcgctctgg tcatagttta gttgtttttc ttcaaacgct
     4081 gttaattgtt cttttttcag ggttaattca taggctaatt ccgttatttg taattcttgt
     4141 tcatgattta cttgtgtcaa ttctttattt ttctgtgcca agttattgtt ttcgatttta
     4201 aggtgcttat tttgcgttct aagcgtctcg aaatctttcg taatactttt tatcgccgaa
     4261 aagtcagaag cgcttaaaac gtattttaaa ccgtttaaac taaatttctt ttctttgaca
     4321 tttgctaaat caggtaaaac ggttgtttgt agagagttaa tgatttcgtt tcgcttcgtt
     4381 tccagttggt tcattgcttg gactttgccc tttacttctt cgttcttctc ttcaagaagg
     4441 cggtcaatac ggaagattgc ctgttcataa gaatggagtt ctctctcttt ttctgccaga
     4501 atttgttcct gattagcgag ttcctgctta gaaacttgta gctccttttg aacgtctttg
     4561 tattctttga cagataaatg cttttgcgga ctcccacgga gtccacgctc aatagcatag
     4621 cctcgttctt gcaaataggt tggcaattct tcttgaatct ctaacaaatt ttgccgacta
     4681 aacacctgtt tactgctcaa tcgcccttcg tacataggca caattcctaa gtgcatatgg
     4741 ggcgtggttt cgtctaaatg taccgtggca taggcgacat tttgtcgacc gcagcgatca
     4801 gagaaatatg cgacaacatc ggtaaaaaat gcttgtgtgt ctgtattctc ttgaaaaaag
     4861 gcggtatcac tagtgattat ccattcgttc acaaggactg cgtcttttct aaccgctcgt
     4921 ttactgattc gttggctctc aatgatttgt ttgattttct cccgataatt gattgaacca
     4981 ggattgacta agtcataatt caagtgagat ttctcaatat caatatcagg attggaatga
     5041 ttattggttt ctcgttgatt atgtcgttgg ataccactta aatttccgtc tttcattttt
     5101 tccatacgtg caacaatttt tgacatga
//
