LOCUS       CP109749               10607 bp    DNA     circular BCT 06-JUN-2023
DEFINITION  Enterobacter hormaechei strain 2017-45-136-01-01 plasmid
            p2017-45-136-01-01_4, complete sequence.
ACCESSION   CP109749
VERSION     CP109749.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN30974098
KEYWORDS    .
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 10607)
  AUTHORS   Rankin,D., Walters,M., Caicedo,L., Gable,P., Moulton-Meissner,H.,
            Chan,A., Burks,A., Edwards,K., McAllister,G., Kent,A., Sula,E.,
            Laufer Halpin,A., Moore,C., McLemore,T., Thomas,L., Dotson,N. and
            Chu,A.
  TITLE     Concurrent Transmission of Multiple Carbapenemases in a Long-Term
            Acute-Care Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10607)
  AUTHORS   Kent,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2022) Division of Healthcare Quality Promotion,
            U.S. Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA 30329, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2019
            Assembly Method                   :: HGAP v. v3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 154.678x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 10/21/2022 13:41:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.3
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,912
            CDSs (total)                      :: 4,796
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 80 of 140
            Pseudo Genes (incomplete)         :: 64 of 140
            Pseudo Genes (internal stop)      :: 11 of 140
            Pseudo Genes (multiple problems)  :: 14 of 140
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10607
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="2017-45-136-01-01"
                     /isolation_source="Sink Area"
                     /db_xref="taxon:158836"
                     /plasmid="p2017-45-136-01-01_4"
                     /geo_loc_name="USA"
                     /collection_date="2018"
                     /note="genotype: ST597"
     gene            join(9863..10607,1..104)
                     /locus_tag="OI979_21985"
     CDS             join(9863..10607,1..104)
                     /locus_tag="OI979_21985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004796809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="WIJ56183.1"
                     /translation="MKYIIYADEAWTHSQPLYRYHRFFGGILSSENSFDDLECEVKKLK
                     KQHNYKKEIKWSNLSARDISFYTELLELVGTFLCKEDHTKYRQMFMDRSYQYTGVNAPD
                     LDSQFKIYYQFLKHIFGLEFSDANSEFLFKLDNHSSYLHKERLREFISSISNDRVKISI
                     EFIESKTSIPLQICDLLMGAAGYYGNKVDWDLLPGKKRRSKNQIMKSEFGKGVYNLLRN
                     IDARFRGRKAFNWFETTGVDGCISNRYHHKMRIWKFIPNGHEKDVSWEDDAFGKNTVRK
                     K"
                     /name="DUF3800 domain-containing protein"
     gene            complement(216..599)
                     /locus_tag="OI979_21990"
     CDS             complement(216..599)
                     /locus_tag="OI979_21990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004796810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WIJ56184.1"
                     /translation="MLTYQPSPGTIVICDFNGFNHPEMVKKRPVVIFAKSNTNPKLVTI
                     VPISTTEPHEITDLHVEVEGPLDGKRAWVKCDMITTICIDRLDRIKIKRGNKTEWGTKQ
                     LNADLLNLVFKGVAKSLGLQHTE"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(810..926)
                     /locus_tag="OI979_21995"
                     /pseudo=""
     CDS             complement(810..926)
                     /locus_tag="OI979_21995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885426.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /name="GNAT family N-acetyltransferase"
     gene            complement(1190..1555)
                     /locus_tag="OI979_22000"
     CDS             complement(1190..1555)
                     /locus_tag="OI979_22000"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WIJ56185.1"
                     /translation="MKRFIKMRNAYAALFLLTSYAASAEVISGPFKDKAECAAFATLAG
                     YDNRISSEQKWGMYESSLRSFDEMRPNLRGSVRFISEYNYEMNVAAIRATDMVIAIKGK
                     RESAAVKLWNQNDCSSK"
                     /name="hypothetical protein"
     gene            complement(1764..2213)
                     /locus_tag="OI979_22005"
     CDS             complement(1764..2213)
                     /locus_tag="OI979_22005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="WIJ56186.1"
                     /translation="MALVSISEAARLTGKSRTTVQSYIKQGKLTKCTDSGGASKLDTSE
                     LLRVFGSFTGQQVGSEQSGSTVQHLAGDFVQLPDQNLLHENYALKAEIELVKALLREKE
                     LLLDEKDKRNEDLKHALLLIESKLPNTSEPVTPHVVKKSWQFWKK"
                     /name="DNA-binding protein"
     gene            complement(2221..3177)
                     /locus_tag="OI979_22010"
     CDS             complement(2221..3177)
                     /locus_tag="OI979_22010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WIJ56187.1"
                     /translation="MAELVVFKANELAVSRYDLTEHETKLILFCVAKLNPTLETPDETE
                     RTVKFSCSEYSSFMGISYENAWGRLNTSTRELFKRSIELIYPTGAVSMRVFNWAEYAEF
                     NRETQEVTLVFSKYIIPLLFHLKRFIKYNLEHVKSFENKYSMRIYEWLLKELTQRKTHK
                     ANISISIDEFKFMLMLEKSYPEYKLLNHWVLKPISKDLNTYSNIKLSIDKRGRPADTLI
                     FQVELDKQIDLVTELAKEPISKKEDNSIRLTPENRLHEGLKTTLHDALTAKIQLTGFEA
                     KFLSDMQSKYDLNGSFTWLTQKQRATLEKILAKYGRN"
                     /name="replication initiation protein"
     gene            complement(3772..4035)
                     /gene="traD"
                     /locus_tag="OI979_22015"
     CDS             complement(3772..4035)
                     /gene="traD"
                     /locus_tag="OI979_22015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WIJ56188.1"
                     /translation="MNLIQAEKRAEARAARKARDHALYQSAGLLILAGLVDSQTGKPVD
                     DTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSSA"
                     /name="traD"
     gene            complement(4098..4703)
                     /locus_tag="OI979_22020"
     CDS             complement(4098..4703)
                     /locus_tag="OI979_22020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0000150 - DNA strand exchange activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WIJ56189.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            4798..7695
                     /locus_tag="OI979_22025"
     CDS             4798..7695
                     /locus_tag="OI979_22025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WIJ56190.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLGTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            complement(7711..7908)
                     /locus_tag="OI979_22030"
     CDS             complement(7711..7908)
                     /locus_tag="OI979_22030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WIJ56180.1"
                     /translation="MNKSEKQEKWAADRVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKGVGSG"
                     /name="hypothetical protein"
     gene            8072..9187
                     /gene="mobA"
                     /locus_tag="OI979_22035"
     CDS             8072..9187
                     /gene="mobA"
                     /locus_tag="OI979_22035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WIJ56181.1"
                     /translation="MASYHLSVKTGGKGNANPHADYIAREGKYAREKDSDLEHKESGNM
                     PAWAAHKPSEFWKAADTSERANGCTYREIEIALPRELKPEQRLELVRDFVRQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERLNDGIPRDPQQYFKRANTKEPERGGAKKARFG
                     ETPTERKEHLVAQRERWADLQNKYLERYQHADRVDARSLKVQGIDREPERHLGAGKVQR
                     FDSAQLQAILERREAERQVQQSRDERDSVIDVTTSLREALAERDSLTPKQEQKSEPEQE
                     AHSGRTFDFEKEPDKLNTLVSDAMKDIQEEIDLQSLVNDAMAEFQGIHQEMERQRERER
                     LVETQRQQEKERQRLAEQKRQKPDKGWSFSR"
                     /name="mobA"
     gene            9200..9784
                     /locus_tag="OI979_22040"
     CDS             9200..9784
                     /locus_tag="OI979_22040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WIJ56182.1"
                     /translation="MSDDIKQIAALIGHHQALEKRVTSLTEQFQDASAQLQQQSDTLSR
                     LIRELDSASGNMTETVRQSVHAALIQVEKELKQAGLAQQKPATEALNQATDTAKAMIHE
                     MRREMSRYTWKSAIYLVLTLFFVLASCVAALTWFMNDGYGQIAQMEAKKAEWERKAPLA
                     NISTCDGKVCVQVTGSEYTNKQGDRFYIIRQ"
                     /name="mobilization protein MobX"
     Typing          complement(2227..3177)
                     /name="rep_cluster_1155"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.376"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          8073..9184
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.062"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcatcataa aatgaggata tggaagttta ttccaaatgg ccatgaaaaa gacgtttcgt
       61 gggaggatga tgctttcgga aaaaatacag tcaggaaaaa gtaaagacaa gggacatctt
      121 tcgatgtccc cggtgcagta ttaacgagct tgtcatcgct actattgtaa atactggctc
      181 atactagatg agtctcacaa gcccctttcg ggtacctatt cagtatgctg taaccctaag
      241 ctttttgcaa cgcctttgaa cactaaattg agaaggtctg catttaattg ctttgtcccc
      301 cattcagttt tgttgcctct tttaattttt attctatcta atcggtcaat gcaaatcgtt
      361 gttatcatat cacattttac ccaagctcgc ttaccatcaa gagggccttc aacctctaca
      421 tgtaaatcag taatctcatg gggttctgtg gtactgattg gtacaatagt gaccaattta
      481 gggttggtat tgctttttgc aaaaattacg acaggccttt tttttaccat ttcgggatga
      541 ttaaacccgt taaaatcgca tatgactata gtgcctggac tcggttggta agtgagcatt
      601 atttatatcc taataaattc cgtatgttac ttcatttaaa ccgtgttttt taacaaaaga
      661 acaatgtaat gattatcata tatctttttg tttataaaat atttttacag gtagacctca
      721 gtattcccca accggaattt ccctaactta agccagattg aacataacat ccctatagcg
      781 cccttccttg gcgctataac agcgttacgt taagggagct ctttgcgcat aaaaacactc
      841 aacggatctg ctttatacgg ggggaatgaa tcgcatatct cgtacccgca tcttcgataa
      901 agcgctatag ccggttgctg gtgaaggggt cagtttggag atcggaaatt tttgtacgtt
      961 aagcgcatta tttaaccaat cttggttcgt aaggggttga ttttgcgtca gtggctatcg
     1021 cttttaagtt gtcgctcttg cagtatcgca tttaggttat ccgattaagg tcaaacctct
     1081 gaaaatgccg tatagcgcgg gagtacaccc tattgctaca ggtaagcctg ttcaaaaaac
     1141 aggcttaccg tacaataatt ctctatatcc aaactgaccc cgtgaatacc tatttcgagc
     1201 tgcaatcgtt ctgattccac aacttcacag cagcactctc gcgctttcct tttatcgcta
     1261 taaccatatc tgtcgcccta attgcagcaa cattcatctc ataattgtat tcactaataa
     1321 accttactga gcctcgcagg ttaggtctca tctcatcaaa actgcgcaat gaactctcat
     1381 acatccccca cttttgttct gatgagattc gattatcgta cccagccaag gttgcaaagg
     1441 cagcacactc agctttgtct ttaaatgggc cagagataac ctctgcgctg gcagcgtagc
     1501 tagtaagtaa aaacaatgcg gcgtatgcat ttctcatttt tataaaacgt ttcacgttat
     1561 ttaccacctc tcggtttcat ataaaaaaaa ccaaatttaa cataagagtt cttgcgcgca
     1621 ccgcccgaac accactcgcc gcaaaaaacc gccggaacgt ccaaaagtac gggttttgct
     1681 gcccgcaaac gggctgttct ggtgttgcta gtttgttatc agaatcgcag atccggtttc
     1741 agtcggtttg ccggctgaaa acgctatttc ttccagaatt gccatgattt tttcaccaca
     1801 tgaggtgtca ctggttccga tgtattcggc agtttagact cgattagcag caaagcatgt
     1861 ttgagatcct cattgcgttt gtctttttca tcgagtagta gctctttctc acgcagcaat
     1921 gcctttacga gttctatttc agcttttaag gcatagtttt catgtagcaa attctggtca
     1981 ggtaactgca caaaatcgcc tgccaaatgc tgcacagtac tgcctgattg ttcactgcca
     2041 acctgctgac cagtaaatga tccgaaaaca cgtagaagct ctgatgtgtc tagttttgaa
     2101 gcaccaccgc tatctgtgca cttggtcagt tttccctgct taatgtatga ctgtactgtt
     2161 gtgcggcttt ttccagttag tctggctgct tcgcttattg atactaaagc catagatacc
     2221 tcagttccgt ccgtacttag ctaaaatttt ctctagcgtg gctcgttgtt tttgagtcag
     2281 ccatgtaaat gagccgttga gatcatactt actctgcatg tcactcagga atttagcttc
     2341 aaatccagta agttgtattt tcgcagttaa agcatcatgt aatgttgttt ttagcccctc
     2401 atgcagacga ttttcaggcg ttaaacggat tgagttatct tctttttttg atattggttc
     2461 ttttgctagc tcagtcacaa ggtcaatttg tttatccagt tcaacttgga agatcagcgt
     2521 atcagcagga cgaccgcgct tatcaataga tagttttata ttgctatagg tatttaaatc
     2581 ttttgaaatt ggttttaaaa cccaatgatt tagtaattta tattctgggt agcttttttc
     2641 taacattagc atgaatttaa actcatcaat gcttattgaa atattggctt tatgtgtttt
     2701 tctttgcgtt agttctttta gcagccattc ataaatgcgc attgagtatt tattttcaaa
     2761 ggacttaaca tgctcgaggt tgtatttaat gaatctcttt aggtgaaata ataatgggat
     2821 tatatattta ctaaaaacta aagttacctc ttgtgtttcc ctattgaact ctgcatattc
     2881 agcccaatta aaaacacgca tagatacagc acctgttgga tatattaatt caatagaccg
     2941 cttaaataac tctcgtgttg atgtatttaa tcgcccccac gcattctcat aacttatccc
     3001 cataaatgaa gaatattcgg agcatgaaaa tttaaccgtt cgttcagttt catctggtgt
     3061 ttctaatgta gggtttagct ttgcaacaca aaacaggatt agtttcgttt cgtgctctgt
     3121 taggtcataa cgactaaccg caagctcgtt tgctttgaat actaccaatt cagccatgac
     3181 taaaaccatt ggttgtgatt ttaaaacaac ataacccttc cgcttggtta tgtcaatttc
     3241 attgccataa ccttttatgt ggttgtgtgg attacctaat tttcacttaa ccttttgaaa
     3301 ggagatccta atttttgctt aacctttcct aattttcact taacctttca tgtgtttttt
     3361 acttataaaa caaaatatta aaatcactag aataaagaaa gaataaaaaa gataaaaaag
     3421 aatagatccc agctttgtgg ataactaacc gccagtgcca gttgagcgat atgataaaag
     3481 gatgtcgcaa acagcgtttg ctcctccaaa aaacatacct tcaaacacga aagaatgagg
     3541 tagcaccctc gcaagctcgg gcaaatcgct gaatattcct ttcgtctccg accatcagac
     3601 acctgagtcg ctgtcttttt cgtgacattc agttcgctac gctcacggct ctggcaggga
     3661 atgggggtaa atgatgctac aggcgctttt catggactca cacgaggaaa tctacccatg
     3721 acacaagaaa agcccgtcac gggcttctca gggcgtttta tggtaggtct gctatgcaga
     3781 gctgtcagac ttcttgctgt tcagcagttc ctgacctctg attttccagt ctgaccactt
     3841 cggattatcc ctcgacaggt cattcagact ggctaatgca cccagcaagg cagcggtatc
     3901 atcaacaggc ttccccgtct ggctgtcaac cagacccgcc aggataagca gtccggctga
     3961 ctggtacagc gcatggtcac gggctttacg ggcggctctg gcttctgccc gtttttcggc
     4021 ctgtatcaga ttcatgactt ttgctctggc ttcctgcgct ttttggggtc ggttccggct
     4081 gagggcgaaa tgacacccta agcgttagct ctgtgtcgtt gcacgatgtc agcgacggta
     4141 ttcttgctga taccgagctc gcgtgcgatc cagcgatagc tgcgtccctc ggccctcatc
     4201 gcaaccacct taggcaaaag tcggtctgat tttggtcgca ctccggcctg acgaccaagc
     4261 ctcttaccac gtgccttcgc aacagcaagg cctgacttga cccgctcgct gatgagatcc
     4321 cgctcaaact ccgcaatgcc ggaaagaaac gtcgccagca ttcgtccata cggcgacgaa
     4381 agatcgaacg ccattccatt catggctatc acggaaacct tccagttctc cagttcacgt
     4441 agcgtattga gcagatcgag cgtcgagcgc ccccaccggg aaagctcagt gaccaggatt
     4501 gcatcaattt gtctggactg ggcaagcgcc aggactttct ttcgctcggc ccggtcgagt
     4561 ttagttcctg aacctgtttc cttaaatatt cccaccacgt cgtagccggc acggccggcg
     4621 aaggctcgca gatcaaattc ctggcgttca caagactgat ccgctgttga aacccggcag
     4681 taaatggcgg cacgatgtcc caattgaacc ctcctggatt tttgtatcgg aacgccctga
     4741 tttatatggg ctggctgttg tccaaaacag actatacttc aaaagggacg aatttgtatg
     4801 tcacgacgcc atattttcac cgaacggcag cgagcagcgc tgttcgatct gcccacggac
     4861 gaactgtcgc tactgaagtt ctacacgctg ggcgatgatg acctggaaaa cattaggcag
     4921 cgccgcagac cggaaaacag gattggcttt gccctgcaac tttgtgcctt acgatatccg
     4981 ggccgtgcac tggctcctgg tgagatgatc ccgcgtgaag tcctttcctt cgtcggtgct
     5041 cagcttggag ttccggctga tgcgcttctc acttatgcca cacggcgcca aacccgtcag
     5101 cagcacatgg acacgctgcg cgaaatttac ggctacaaga ccttcacggg ccgtggtgcc
     5161 cgtgatctgc gggagtggac tttcggccag gccgaagatg ccagatcaaa cgaggatctt
     5221 gctcatcgtt ttattgtgcg gtgtcgggaa acttccacca ttctgcccgc agtatcgaca
     5281 atcgagcgct tgtgcgcgga tgctctggtc gccgctgagc ggcggattga aacgcggatt
     5341 gcggaaaatt taacagcgga tgttcgcgat cacctggaca aacttctgag tgaaatgctc
     5401 gccggcaata tcagtcgttt catctggctt cgcaacttcg aggttggtaa caactcggct
     5461 gctgctaacc gtttgctcga caggctcgaa tttctgcgta ccctgaatat caatcatagt
     5521 gctttggcca gcatacctgc ccatcgcatt gcccggctgc gtcggcaggg tgaacgctac
     5581 ttcaccgacg gtttgcgtga catcacttcg gaccgccgct gggcgatcct tgccgtctgt
     5641 gttgtggagt gggaagcggc gattgctgat gccatagtcg aaacccatga caggatcgta
     5701 ggaaaaacct ggcgggaagc gaagcgccaa catgacgaaa caatttccgg ctctaaagcc
     5761 acactcacgg atacgatccg taccttcacc gcgctgggag cttcgttgct tgaggcccgc
     5821 agtgacggaa ccccgctgga gatggctgtc gccagttcgg ttgcatggga ccggctcgct
     5881 caactggtag cgacagggac tcaactcagc aacacgctag ccgatgagcc tcttgcatat
     5941 gtcgggcagg gataccatcg ctttcgtcgt tatgcgcccc gcatgttgcg ctgtctgaag
     6001 ctcgaagccg cgccggtcgc cggaccattg gtagcagcag ctttgtcgat cggagagatg
     6061 aaaggtgttg catcgccaga aaggcgtttc ctgcggccca gctccaaatg gaaccgtcat
     6121 ttacgagctc aggaaaaagg agatacccgt ctttgggaag tggcggtact ctttcacctc
     6181 cgggatgctt ttcgttccgg agatgtctgg ctcgctcatt cgcgccgcta tggtgacctc
     6241 aagcaggtac tggtgccgat gatcgcggcg caggaaaatg caaaactggc cgtgccttcc
     6301 aacccacagg attggctggc agacagaaag gcgcgactca cgatcgctct taagcggctg
     6361 gcccgggctg cccgtaacgg cactattccg cacggtagca tagaagatgg aacgttgcgg
     6421 atcgacaggt tgacagcaga cgtgccggat ggtgccgagg cactcatact ggatctgtat
     6481 cgccgaatgc cgtccgttcg gattaccgac atgctgcttg aagttgatgc agcccttggt
     6541 ttcacagatg cgtttaccca tctaggaacc ggggctccat gtcgcgaccg gatcggtctg
     6601 ctcaacgtcc tgctcgctga agggctcaat ctgggcctgc gtaagatggc ggaagctaca
     6661 aacacgcatg attactggca gctctcacgc cttgcccgct ggcatgttga aagcgaagcc
     6721 atgaaccagg cattggcaat tgtggtggcc gcgcagggta aactgccgat gtcacgcgtc
     6781 tgggggatgg gcacgtcagc atcgagcgat ggtcagtttt tcccgacagc gcggcatggc
     6841 gaagccatga acatggtcaa tgccaaatat ggttctgttc ccggcctcaa agcgtatact
     6901 cacgtaagcg accagttcgc gccattcgct tgtcagtcga tcccggcgac cgtgagcgag
     6961 gcaccgtata ttctcgatgg actactgatg aacgaggtcg gtcgccatgt tcgcgaacag
     7021 tatgccgata cagcaggatt caccgaccat ttgttcggag ccagtagcct gctcggctac
     7081 aatctcgttc tgcgaatcag ggatctgcca tcgaagcggt tgtacgtatt taatcccgat
     7141 acgaccccca gggagttacg caagttggta ggtggaaaag cccgggagga tcttatcgtt
     7201 gcgaactggc ctgatatttt ccgttgtgcc gcgacgatga ccgctggcaa aatcaggccc
     7261 agccaactcc tgcgcaagct cgcttcttac ccacgacaaa acaaccttgc agttgcgctt
     7321 cgtgaagttg gtcgtattga acggaccctt ttcattattg agtggatcct ggatacggac
     7381 atgcagcggc gtgctcagat cggtcttaac aagggagagg cccaccatgc gctcaaaaat
     7441 gcgctccgta tcgggaggca gggggaaatt cgcgatcgca cgacagaggg gcagcactac
     7501 cgaatcgctg ggctcaattt attgactgcg gtgatcattt actggaatac cgtccatctt
     7561 ggtcatgccg tcacggagcg gcggaacgaa gggttggatg ttccccctga atttcttccc
     7621 cacatatccc cattgggctg ggcgcacatt ctactgactg gcgaatatct ttggcccaag
     7681 gaaccgaaag cttagggtgt catttcgccc tcagccggaa ccgacccctt tttctgctgc
     7741 ctgttcagcc ttcatcagca gggacagcgt tttgatatcc tgcgctgttt tatccgcttt
     7801 atccgtcagt atcagcatga gtttctgctg ttcattcggc gactttaagc cgcgaatgta
     7861 ctgcactctg tctgctgccc atttttcctg tttttcgctt ttattcatgc ccatttatcc
     7921 tctgaatgtt gatgtggaaa ttatggtcag gaatggcggc cgaggtcaaa tgcaggtaga
     7981 atcactgata agctttttat tatcagtgcg cacttagacg tttcttcact tcgttcagaa
     8041 acggtgcgcg ccttcagcgg gataaaacac gatggcatcc tatcacctta gcgtaaaaac
     8101 cggcgggaaa ggcaatgcca atccccatgc cgattatatt gcgcgtgagg gaaaatatgc
     8161 ccgtgaaaaa gactccgacc ttgagcataa ggagtccgga aatatgcccg catgggcagc
     8221 gcataaaccg tccgagttct ggaaagctgc tgacacgtct gaacgtgcta acggctgcac
     8281 ctaccgcgaa atcgagattg ccctgccgcg tgagctaaaa cccgaacaac gccttgagct
     8341 tgtccgcgat tttgtccggc aggaaatcgg cgaccgccat gcttatcagt ttgccattca
     8401 taacccgaaa gcggcgatag ccggcggcga gcagccgcac gcgcatatca tgttctctga
     8461 acgcctcaat gacggcattc cccgtgaccc gcagcagtat ttcaaacggg ctaataccaa
     8521 agagccggag cgcggtggcg cgaaaaaggc acgtttcggg gaaacaccta ccgaacgtaa
     8581 agagcacctt gttgcccagc gggaacgctg ggctgacctc cagaataagt acctggaacg
     8641 gtatcagcac gctgaccgtg ttgatgcccg ttcactcaaa gtgcagggca ttgatcgtga
     8701 gccggagcgg catcttggcg cggggaaggt tcagcgattc gattcggcgc agttacaggc
     8761 cattcttgag cgcagggagg ctgaaaggca ggtgcagcag tcccgtgatg aacgtgatag
     8821 cgtaattgac gtgaccacct cgctgcgcga ggcactggcg gagcgggata gtctgacgcc
     8881 aaaacaggaa cagaagtcag agcctgagca ggaagcccat tccggcagaa cgtttgattt
     8941 tgaaaaagag cctgacaagc tgaacacact ggtcagtgat gccatgaagg acatacagga
     9001 agaaatcgat cttcagtccc ttgttaacga tgccatggcg gagtttcagg ggatacatca
     9061 ggaaatggaa cggcagagag agcgtgagcg cctggtggaa acacagcgtc aacaggagaa
     9121 agagcggcag cgactcgccg aacaaaaacg acagaagccg gataaaggct ggtcattttc
     9181 acgatagaca ggattttgta tgagcgacga cattaaacaa atcgctgccc tcatcgggca
     9241 tcatcaggcg ctggaaaagc gtgttacctc tctgactgag cagtttcagg acgcctcagc
     9301 acaattacag cagcagagtg acacgctgag caggctcatt cgtgagctgg acagcgcgtc
     9361 cggtaatatg actgaaaccg tcaggcaatc tgttcatgcc gcgctcattc aggttgagaa
     9421 agagctgaaa caagccggat tagcacagca gaaaccggcg acagaagcgc ttaatcaggc
     9481 taccgatacg gcgaaggcga tgatccatga aatgcgacgc gagatgtcgc gctatacgtg
     9541 gaaatctgcg atttatcttg ttctgacact gttcttcgtc ttggcgtcct gcgtggctgc
     9601 gctgacgtgg tttatgaatg atgggtacgg acagatagct caaatggagg caaaaaaagc
     9661 tgagtgggaa aggaaagctc cgctagctaa catcagcaca tgtgatggta aggtatgcgt
     9721 acaggttaca ggttctgagt atacaaacaa acaaggtgat aggttttaca taatcaggca
     9781 atgagtttat gccttggata tcattttgaa cggtgtatca ttatctgtag ttgattgatt
     9841 catttagaaa tgagagggta tcttgaagta tatcatatat gcagatgagg catggactca
     9901 ttcccagcca ctttatagat accatcgttt ttttggtggt atcttatcat cagaaaatag
     9961 ttttgatgat cttgaatgcg aagtaaagaa actaaaaaaa caacataact ataagaaaga
    10021 aataaagtgg tctaacctct cggcgagaga tatttctttt tacactgaat tgctcgagtt
    10081 agttgggacg tttctttgca aagaagatca taccaagtac aggcaaatgt ttatggatag
    10141 atcatatcaa tatactggcg tcaatgctcc tgatcttgat tcgcaattta aaatatacta
    10201 tcagttttta aaacatattt ttggtctcga gttttcagat gctaactctg agttcctatt
    10261 taaactagat aatcactcta gttatttgca taaagagaga ctaagagaat tcatatcctc
    10321 tattagtaac gatagagtta agataagcat agagtttata gaaagtaaga catcaatccc
    10381 tttgcaaatt tgtgatttgt tgatgggggc tgcaggttat tacggaaata aagtcgactg
    10441 ggacctattg cctggaaaaa agaggcgctc taaaaatcaa ataatgaaat cagaatttgg
    10501 gaagggggta tataatttac ttagaaatat tgatgcaagg ttcagaggta gaaaggcttt
    10561 taactggttt gaaactacgg gtgtagacgg ctgtataagt aatagat
//
