LOCUS       CP104434               20384 bp    DNA     circular BCT 22-SEP-2022
DEFINITION  Shigella sonnei strain 1519721 plasmid p1519721_4, complete
            sequence.
ACCESSION   CP104434
VERSION     CP104434.1
DBLINK      BioProject: PRJNA315192
            BioSample: SAMN22962688
KEYWORDS    .
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20384)
  AUTHORS   Greig,D.R., Painset,A. and Jenkins,C.
  TITLE     Nanopore sequencing of Shigella species
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20384)
  AUTHORS   Greig,D.R., Painset,A. and Jenkins,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2022) Gastrointestinal Bacteria Reference Unit, UK
            Health Security Agency, 61 Colindale Avenue, London NW9 5EQ, United
            Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2022
            Assembly Method                   :: Flye v. 2.9
            Assembly Name                     :: 1519721
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 52x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 09/14/2022 18:52:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,359
            CDSs (total)                      :: 5,233
            Genes (coding)                    :: 4,384
            CDSs (with protein)               :: 4,384
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 849
            CDSs (without protein)            :: 849
            Pseudo Genes (ambiguous residues) :: 0 of 849
            Pseudo Genes (frameshifted)       :: 331 of 849
            Pseudo Genes (incomplete)         :: 523 of 849
            Pseudo Genes (internal stop)      :: 129 of 849
            Pseudo Genes (multiple problems)  :: 124 of 849
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..20384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="1519721"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /plasmid="p1519721_4"
                     /geo_loc_name="United Kingdom: United Kingdom"
                     /collection_date="2021-10"
                     /collected_by="PHE"
     gene            join(20072..20384,1..11)
                     /locus_tag="LNB82_26645"
     CDS             join(20072..20384,1..11)
                     /locus_tag="LNB82_26645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UXF43670.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            220..657
                     /locus_tag="LNB82_26650"
                     /pseudo=""
     CDS             220..657
                     /locus_tag="LNB82_26650"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            689..1249
                     /locus_tag="LNB82_26655"
     CDS             689..1249
                     /locus_tag="LNB82_26655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UXF43671.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYLEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            1256..1489
                     /locus_tag="LNB82_26660"
     CDS             1256..1489
                     /locus_tag="LNB82_26660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UXF43685.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1973..2395)
                     /locus_tag="LNB82_26665"
     CDS             complement(1973..2395)
                     /locus_tag="LNB82_26665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="UXF43672.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            2517..2795
                     /locus_tag="LNB82_26670"
     CDS             2517..2795
                     /locus_tag="LNB82_26670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UXF43673.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KIFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(2787..3077)
                     /locus_tag="LNB82_26675"
     CDS             complement(2787..3077)
                     /locus_tag="LNB82_26675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UXF43674.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            3317..4963
                     /gene="cka"
                     /locus_tag="LNB82_26680"
     CDS             3317..4963
                     /gene="cka"
                     /locus_tag="LNB82_26680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="UXF43675.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEVKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            complement(4960..5250)
                     /gene="cki"
                     /locus_tag="LNB82_26685"
     CDS             complement(4960..5250)
                     /gene="cki"
                     /locus_tag="LNB82_26685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="UXF43676.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            5280..5411
                     /locus_tag="LNB82_26690"
     CDS             5280..5411
                     /locus_tag="LNB82_26690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="UXF43677.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5606..5749)
                     /locus_tag="LNB82_26695"
     CDS             complement(5606..5749)
                     /locus_tag="LNB82_26695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UXF43678.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(6415..6576)
                     /locus_tag="LNB82_26700"
     CDS             complement(6415..6576)
                     /locus_tag="LNB82_26700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UXF43679.1"
                     /translation="MARFIRSQTLTLLEKLNELDADDQADICEALHDHADELYRSCLAR
                     FGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6673..6996
                     /locus_tag="LNB82_26705"
                     /pseudo=""
     CDS             6673..6996
                     /locus_tag="LNB82_26705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7314..7640
                     /locus_tag="LNB82_26710"
                     /pseudo=""
     CDS             7314..7640
                     /locus_tag="LNB82_26710"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            7669..8226
                     /locus_tag="LNB82_26715"
     CDS             7669..8226
                     /locus_tag="LNB82_26715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UXF43680.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFWRHGADSVAGKPDCGEHPGYQGPGRDAGETPGKNMGCDVSRGQEREISGVA
                     VRGEGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            8233..8466
                     /locus_tag="LNB82_26720"
     CDS             8233..8466
                     /locus_tag="LNB82_26720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UXF43682.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(8947..9369)
                     /locus_tag="LNB82_26725"
     CDS             complement(8947..9369)
                     /locus_tag="LNB82_26725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="UXF43681.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(9757..10090)
                     /locus_tag="LNB82_26730"
                     /pseudo=""
     CDS             complement(9757..10090)
                     /locus_tag="LNB82_26730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349482.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            10286..11927
                     /gene="cka"
                     /locus_tag="LNB82_26735"
                     /pseudo=""
     CDS             10286..11927
                     /gene="cka"
                     /locus_tag="LNB82_26735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /name="cka"
     gene            complement(11924..12214)
                     /gene="cki"
                     /locus_tag="LNB82_26740"
     CDS             complement(11924..12214)
                     /gene="cki"
                     /locus_tag="LNB82_26740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="UXF43663.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKNHWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            complement(13008..13199)
                     /locus_tag="LNB82_26745"
     CDS             complement(13008..13199)
                     /locus_tag="LNB82_26745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UXF43664.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            13264..13572
                     /locus_tag="LNB82_26750"
     CDS             13264..13572
                     /locus_tag="LNB82_26750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UXF43683.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGVRSSDTGTAAVA
                     SVRLVGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGRELSELRDEVRREQGARDDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            13562..14122
                     /locus_tag="LNB82_26755"
     CDS             13562..14122
                     /locus_tag="LNB82_26755"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UXF43665.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELID
                     ATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLE
                     LNFVIPNMELQTGKRLQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLVTPITC
                     RKTKQEAAEAITRDWRRFTMPER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            14656..14889
                     /locus_tag="LNB82_26760"
     CDS             14656..14889
                     /locus_tag="LNB82_26760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UXF43684.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(15373..15794)
                     /locus_tag="LNB82_26765"
                     /pseudo=""
     CDS             complement(15373..15794)
                     /locus_tag="LNB82_26765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /name="Exc2 family lipoprotein"
     gene            complement(16185..16475)
                     /locus_tag="LNB82_26770"
     CDS             complement(16185..16475)
                     /locus_tag="LNB82_26770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UXF43666.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            16761..18361
                     /gene="cka"
                     /locus_tag="LNB82_26775"
                     /pseudo=""
     CDS             16761..18361
                     /gene="cka"
                     /locus_tag="LNB82_26775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /name="cka"
     gene            complement(18358..18647)
                     /gene="cki"
                     /locus_tag="LNB82_26780"
                     /pseudo=""
     CDS             complement(18358..18647)
                     /gene="cki"
                     /locus_tag="LNB82_26780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /name="cki"
     gene            18677..18808
                     /locus_tag="LNB82_26785"
     CDS             18677..18808
                     /locus_tag="LNB82_26785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="UXF43667.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(19003..19146)
                     /locus_tag="LNB82_26790"
     CDS             complement(19003..19146)
                     /locus_tag="LNB82_26790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UXF43668.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(19813..20004)
                     /locus_tag="LNB82_26795"
     CDS             complement(19813..20004)
                     /locus_tag="LNB82_26795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UXF43669.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          6244..6329
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.73e-38"
     Typing          12833..12914
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          19642..19727
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.73e-38"
     Typing          complement(5678..6232)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.416"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(12262..12801)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.714"
                     /coverage="100.0"
                     /evalue="1.3399999999999999e-158"
     Typing          complement(19075..19630)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.774"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..1596
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.624"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6413..6771
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.5"
                     /coverage="100.0"
                     /evalue="7.440000000000001e-73"
     Typing          6769..6977
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="7.440000000000001e-73"
     Typing          7129..8573
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="81.136"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          13563..14089
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.182"
                     /coverage="99.0"
                     /evalue="2.41e-102"
     Typing          14587..14996
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.54"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          19965..20383
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.571"
                     /coverage="100.0"
                     /evalue="1.87e-75"
ORIGIN
        1 atgatagtta agtttcatgc caggggaaaa ggcggtggca gtggtccggt tgattacctg
       61 ctgggccggg agcgtaaccg ggaaggggcg agagttctgc ggggtgctcc cgaagaggtg
      121 cgggaactca tcgatgccac gccgtttgcg aaaaaataca cctcgggggt tctgtctttt
      181 gcagagcaga ccctgccacc gggagagcgt gaaagggtga tggagagctt tgagcgggta
      241 ctgatgcccg gtcttgaaaa aaatcagtac agcatcctgt gggtggagca ccaggacaag
      301 gggcgtcttg agctgaattt tgtcatcccg aatatggagc tggcgagcgg aaaacggctc
      361 cagccctatt acgaccgagc ggaccgtccg cgaatcaatg cctggcagac actggtgaat
      421 caccattacg ggctgcacga cccgaacgca ccggagaaca ggcggacact tgtcaccccg
      481 aataatctgc cgaaagcgaa acaggaggcc gcagaagcga ttacgagggg actggaggct
      541 ctttaccatg ccggagcgct aaaaacgcgt caggatgtca ctgaggcgct tacagcggca
      601 ggttttgagg ttgtgaggac aaccaggagc agtatcagta tcgccgaccc ggagggtggg
      661 cgtaatctca ggctgaaagg ggcgctgtat gagcaatctt ttacaaatgg ggacagactt
      721 cgagaagaaa ctgaaagagc gtgccgtctc tacagagaac atgctgaaca gcgagttcag
      781 gaagctcgaa gcgtctgtag acagggaact gaacttaaac gggcagaaaa tcagagacgc
      841 tatcagcgca cacacagcag cggtgaaaga acaactggag acgctgaaca gtgccgtgaa
      901 cgcgcagttc agcgccacag agaacgaact ggcccggcgg caggaagagc tgctctggaa
      961 gctggtcaaa gggcggatac tgtacccgtc gctgacggcg ctttgcgtca cgggcgggat
     1021 ttttctggcg tcatgggggc tgattcagtg gcaggaaagc cggattgcgg cgaacatcct
     1081 ggctatcagg gaccaggaag agacgctggc gaaactccgg gaaaaaacat ggggtgtgac
     1141 gtatctcgag ggcaggaacg ggaaatttct ggtgttgccg tccggggtga agggggaaaa
     1201 caactggacg gtggagaaga agaacgcagt aagactggtc agggagtaag tgagtatgac
     1261 agagctggaa atgcacttac tgaacgcctt agagcaattg cagcaggact atacgcagcg
     1321 gctgaaagac tgggagagcg ccttcacgga tttgcagcat atgttcgagc tgacacgaca
     1381 ggagaacggg caactgaggg aacagtgcga cgacttgagt cagcaggtgc agagattagc
     1441 cgggcaggtg gaccacttga gtcggttatt tatcacgaac agcagataag ggatgaacag
     1501 gaaaggcagg cacgcctgga acgggagcgt gcgcggtcct ggcatgaaga gcaggaacgg
     1561 gaagcacggg tatggcgtgg cccgtcaata gggatgtgac ggtgagttgt tccgggaggt
     1621 tgccagatgg taccggaacg cgttaaaaac gcatccgggc gcgattctgg cgtgtcgttt
     1681 ttgttgctgt gttttgcctc tttgccagat cgcgctcatt cctcagcctc atcccgcgat
     1741 gtctgcgtat gtcatcaact gaaaagatgg aaaaaaaaca gtgtcatgag gtttacgcag
     1801 gcaaaaccag cgttattcac atggctgaat agctatgcat acggttaaaa tttatcaggt
     1861 gcgatcgcgg cagcttttcg ggtggtttgt tgccattttt acctgtcagc agccgtgatc
     1921 gcgctgaacg catttcagcg gtgcgtacaa ttaagggatt atggtaaatc ccttactgtc
     1981 tgccctcgta tccatcgaga taaaccgcag aaatggtgtc tgccattagt ttcatttttt
     2041 tcggcgaagg catatcgctg tttgtgtatt ttctgccagc aaaggctgtt gtgctccgga
     2101 gtgagtgaaa aaatttttct gagtggaatt cttttacacg ttgctgtgcc gtttctgctg
     2161 atactccggc tgctttgtct ttcatcccca tatcccggaa ctgctgaaag aacgggagca
     2221 tcatacgtat ggtgtctgct ttctgggtgt agaagttagg atcaaatcca tcatctgaag
     2281 cataaacata atgccgggta tgtcgttcca ggctggtctt tggtgtgcat cctgtaagag
     2341 ctattgttgc tgttatcaga ggtatcagaa gcagcctggc agcggcagat ctcataaaca
     2401 tctccttgcg tttttaatct tgatactcag ggagttatat ctccgaagag tatctcttat
     2461 catacgtcgg tttactccca atgtgatttg atggtcaatg agtcaggaat aatcccgtga
     2521 aaaaatgtgt gccagtcctg ctttttagtg ttctttctgc ttcggttgcc agtcatgcgg
     2581 acagcaatgt ttttcaacaa aaggccgaga agacaaaaaa cggcattgtt gcggatgtta
     2641 atgccagaca gaaaatattc agtaacagac tgaagataca acttcagatc gatgagttga
     2701 gagcagagct ggctggcacc agagatgccc aaaaaagaga ggatttgcgt aaaaaactgg
     2761 agtctctgga gcggcaaaag aaatccttac gctgatgggg gcgtattttc cccggtttct
     2821 tccataacct ttttaccgtt cagatattta cgggcgaggc gaatgcgtct tgcctggtat
     2881 accgccgagt aagcctgggt gggattcaca ccattactga tttcactgac cacatgacgc
     2941 tgatcagcca gtgtcagcgt gtaaacctgt ttctggatga ggtcacgggc tttgtcatgc
     3001 tcatatccgg cagcaacaag ggtgtcgaga agtcgtttgt ctgttttgtt cagtttatgg
     3061 cggggtgatt tgctcacatc gttctccgtt ttttcatgat ccaacagttt tatatctcat
     3121 cctgatttgc tccggtgttc tgtttcgacg ttctcaatgc ctgcctcccc atagtgtgat
     3181 ccttattgca aaaatgaaaa tccatgctct tgacatggac aatactgagt agtaggtttt
     3241 actgtacata aaaccagtgg ttatatgtac agtatattgt ttttaattta ttgttttaat
     3301 tatcaaagag gaatttatgg ctaaagaact aagtggatat ggaccaactg ctggtgagtc
     3361 tatgggggga acaggggcga atctgaatca gcaaggtgga aataataata gtaatagtgg
     3421 ggttcactgg ggtggtggct ctgggcatgg taataatggt ggtcagggaa attcaaattc
     3481 atctggttct acatcaactg tgatgaaaac cggtgagtca tatctgactc cgtggggtga
     3541 tgttgtcatt aataatgatg gccttccggt gatgaatggt attgttatga cagaagagaa
     3601 ttcaactctg gttgataatc catttggagg ggtctccaga gttcttaatt ctttaattag
     3661 tgatatgcca tcactttttg cagaaagctc aggaaataac aacaataaca ctgcttcagt
     3721 taatactgca ccaacgaatg ctcaggtcag tgatatggat aagagttcga aggtggtggg
     3781 taatgtaatt aatgaaaaac aaaagcagaa gaataaaatt gctacacaga ttagtgaaaa
     3841 acagaagaaa atcgaggaga tgaaaaaagt attcaagcat cattcttatc atggtatcac
     3901 tgatcttgag agggatgttg atgagttgca aaaaaaatca aatcagcttg atgcagatat
     3961 tagcaaactt aattcttata aaaatacatt gcaaagtaaa attggtgatg tgaataaaca
     4021 gaaggaagct gaagaaaagg ctcgtgaaaa tgcagaggtc gctgaacatg aaactcttaa
     4081 tgaagagaaa caggcggtgg cggaagcaga gaagcgttta gctgaggtga aagctgaact
     4141 ggcgaaggct gaaagtgatg tacagagtaa gcaagctact gtttccagag ttgcaggaga
     4201 gcttgaaaac gctcagaaga gtgttgacgt gaaggtcacc ggatttccgg gatggcgtga
     4261 cgttcagaaa aaactacagc gtcagcttga agctaagcag gctgagtact ccgcagttga
     4321 aaatgaactt aagaatgctg ttagtttcag ggatggtaag gccgctgagg ttaaagaggc
     4381 agagcagaaa ttaaaagaag ctcaggatgc tcttgaaaag agccagataa aagatgctgt
     4441 tgatactatg gttggatttt atcaatatat aaccgaacaa tatggagaaa aatacgcaaa
     4501 aattgctcag gatttagcgg aaaagtctaa aggaaagaaa attcaaggtg ttgatgaagc
     4561 tctggcagca tttgaaaagt ataagaatgt actggataag aaattcagta aggttgatcg
     4621 ggatgctatt tttaatgcgt tagagtctgt taattatgat gagttatcta agaatttaac
     4681 gaagatatca aagtcgctta aaatcaccag tcgagtatct tttctatatg atgttggttc
     4741 tgattttaaa aatgcaatag aaactggtaa ctggcgacct ttgtttgtca cgctggagaa
     4801 aagtgcggtc gatgtaggtg tggcaaaaat tgtggcatta atgtttagtt ttattgttgg
     4861 tgtgcctctt ggtttctggg ggattgcaat tgtcacaggt attgtttctt cttacatagg
     4921 ggatgatgag ttgagcaaac ttaatgagtt gttagggatt taatttctct ttagagtgta
     4981 atatatgaaa cttggcaatc ctactgggat tgccattaat aaataaaaca atgcagataa
     5041 cttgcataag taccagggtt ctttttttaa ctttgtattt tctttgatat atctagatat
     5101 ggttaattta gagtatggat atagcaatac atgtattgat gatataaata taaataacaa
     5161 aggagtgtta tcattgtcgt taaatattaa aataagaatc catggtataa gtgattcagg
     5221 taaattatgt aggtagtatt ttaaatgcat aagtattttt acttttgttg gaaagaaata
     5281 tgaaaataat aatttttcta attacaatat tattcttgtc aggatgtcaa gcaaactata
     5341 ttcgtgatgt acaaggagga accgttgcac catcttcgtc ttctgaactg acggggatcg
     5401 cggttcagta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5461 ctgcctgtaa acgaaaaaac cgcctgggga ggcggtttag tcgaaggtta agtcagttgg
     5521 ggactgctta accgggtaac tggctttagt ggagcacaga taccaaatgc tgtccttcca
     5581 gtgtagccgc tataaggctt caacttcaag agttctgcgt atctaaatcc actatccagt
     5641 taccaatggc tgctgccagt ggcgttaagt cgtgtcttac cgggttggac tcaagacgat
     5701 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5761 tggagcgaac gacctacacc gaactgagat accaacagcg tgagctatga gaaagcgcca
     5821 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5881 agcgcacgag ggagccacca gggggaaacg cctggtatct ttaagtcctg tcgggtttcg
     5941 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6001 aaacgccacc tgcgcggcct tttccgggtt cttgtttttg ctcacatgtt tcttttggtg
     6061 ttatcccctg attctgtgga taacagcatt tcgtttttga gtgagtagat accgctcgcc
     6121 gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga agcggaaaag cggctggacg
     6181 tgcattttct ccttacgcat ctgtgcggca tttcacaccc ggtatggcgc acttttcata
     6241 ccatccgcac tgatgccgca tggttaagcc agtatacact ccgctatcgc tacgtgactg
     6301 ggtcatggct gcgccccgac acccgctaaa acccgctgac gcgccctgac gggcttgtct
     6361 gctcccggca tccgctcaca ggccagctgt gaccgtctcc gggagctgca tgtgtcagag
     6421 gttttcaccg tcatccccga agcgtgcgag gcagctgcgg taaagctcgt cagcgtggtc
     6481 gtgaagcgct tcgcagatgt cagcctggtc gtcggcgtcc agttcattca gtttttccag
     6541 cagggtcagc gtctggcttc tgatgaagcg ggccatgtta agggcggttt ttcctgttta
     6601 gtcacagttc tgtttctcca tgtgtgggga gtctgtcgga ggtttttaat gataccggtg
     6661 aaacgcgaaa ggatgctcac gatacgggtc acggatgagg aacatgcgcg gctgctggca
     6721 cgttgtgagg gaaaacaact tgcgtcatgg atgcgtaagg tctgcctggg gcaccgccat
     6781 cgaaaacgtc aggtcttccg acactggcac cgccgctgtt gcgccagttc gcttcggtcg
     6841 gcaataacct gaatcagata gcccggaaga taaacagcgg acagtggtcg gggcatgacc
     6901 gggttcatgt ggtggctgca ctgatggcca tcggacgtga actgagtgag ctgagggatg
     6961 aggtccgtaa aacagggaga acgggatgat agttaagttt catgccgggg aaaaggcggt
     7021 ggcagtggtc cggttgatta cctgctgggc cgggagcgta accgggaagg ggcgagagtt
     7081 ctgcggggtg ctcccgaaga ggtgcgggaa ctcatcgatg ccacgccgtt tgcgaaaaat
     7141 acacctcggg ggttctgtct ttgcagagca gaccctgcca ccgggagagc gtgaaagggt
     7201 gatggagagc tttgagcggg tactgatgcc cggtcttgaa aaaatcagta cagcatcctg
     7261 tgggtggagc accaggacaa gggcgtcttg agctgaattt tgtcatcccg aatatggagc
     7321 tggcgagcgg aaaacggctc cagccctatt acgaccgagc ggaccgtccg cgaatcaatg
     7381 cctggcagac actggtgaat caccattacg ggctgcacga cccgaacgca ccggagaaca
     7441 ggcggacact tgtcaccccg aataatctgc cgaaagcgaa acaggaggcc gcagaagcga
     7501 ttacgagggg actggaggct ctttaccatg ccggagcgct aaaaacgcgt caggatgtca
     7561 ctgaggcgct tacagcggca ggttttgagg ttgtgaggac aaccaggagc agtatcagta
     7621 tcgccgaccc ggagggtggg cgtaatctca ggctgaaagg ggcgctgtat gagcaatctt
     7681 ttacaaatgg ggacagactt cgagaagaaa ctgaaagagc gtgccgtctc tacagagaac
     7741 atgctgaaca gcgagttcag gaagctcgaa gcgtctgtag acagggaact gaacttaaac
     7801 gggcagaaaa tcagagacgc tatcagcgca cacacagcag cggtgaaaga acaactggag
     7861 acgctgaaca gtgccgtgaa cgcgcagttc agcgccacag agaacgaact ggcccggcgg
     7921 caggaagagc tgctctggaa gctggtcaaa gggcggatac tgtacccgtc gctgacggcg
     7981 ctttgcgtca cgggcgggat tttctggcgt catggggctg attcagtggc aggaaagccg
     8041 gattgcggcg aacatcctgg ctatcaggga ccaggaagag acgctggcga aactccgggg
     8101 aaaaacatgg ggtgtgacgt atctcgaggg caggaacggg aaatttctgg tgttgccgtc
     8161 cggggtgaag gggaaaacaa ctggacggtg gagaagaaga acgcagtaag actggtcagg
     8221 gagtaagtga gtatgacaga gctggaaatg cacttactga acgccttaga gcaattgcag
     8281 caggactata cgcagcggct gaaagactgg gagagcgcct tcacggattt gcagcatatg
     8341 ttcgagctga cacgacagga gaacgggcaa ctgagggaac agtgcgacga cttgagtcag
     8401 caggtgcaga gattagccgg gcaggtggac cacttgagtc ggttatttat cacgaacagc
     8461 agataaggga tgaacaggaa aggcaggcac gcctggaacg ggagcgtgcg cggtcctggc
     8521 atgaagagca ggaacgggaa gcacgggtat ggcgtggccc gtcaataggg atgtgacggt
     8581 gagttgttcc gggaggttgc cagatggtac cggaacgcgt taaaaacgca tccgggcgcg
     8641 attctggcgt gtcgtttttg ttgctgtgtt ttgcctcttt gccagatcgc gctcattcct
     8701 cagcctcatc ccgcgatgtc tgcgtatgtc atcaactgaa aagatggaaa aaacagtgtc
     8761 atgaggttta cgcaggcaaa accagcgtta ttcacatggc tgaatagcta tgcatacggt
     8821 taaaatttat caggtgcgat cgcggcagtt ttcgggtggt ttgttgccat ttttacctgt
     8881 cagcagccgt gatcgcgctg aacgcatttc agcggtgcgt acaattaagg gattatggta
     8941 aatcccttac tgtctgccct cgtatccatc gagataaacc gcagaaatgg tgtctgccat
     9001 tagtttcatt tttttcggcg aaggcatatc gctgtttgtg tattttctgc cagcaaaggc
     9061 tgttgtgctc cggagtgagt gaaaaaattt ttctgagtgg aattctttta cacgttgctg
     9121 tgccgtttct gctgatactc cggctgcttt gtctttcatc cccatatccc ggaactgctg
     9181 aaagaacggg agcatcatac gtatggtgtc tgctttctgg gtgtagaagt taggatcaaa
     9241 tccatcatct gaagcataaa cataatgccg ggtatgtcgt tccaggctgg tctttggtgt
     9301 gcatcctgta agagctattg ttgctgttat cagaggtatc agaagcagcc tggcagcggc
     9361 agatctcata aacatctcct tgcgttttta atcttgatac tcagggagtt atatctccga
     9421 agagtatctc ttatcatacg tcggtttact cccaatgtga tttgatggtc aatgagtcag
     9481 gaataatccc gtgaaaaatg tgtgccagtc ctgcttttta gtgttctttc tgcttcggtt
     9541 gccagtcatg cggacagcaa tgtttttcaa caaaggccga gaagacaaaa aacggcattg
     9601 ttgcggatgt taatgccaga cagaaaatat tcagtaacag actgaagata caacttcaga
     9661 tcgatgagtt gagagcagag ctggctggca ccagagatgc ccaaaaagag aggatttgcg
     9721 taaaaactgg agtctctgga gcggcaaaag aaatccttac gctgatgggg gcgtattttc
     9781 cccggtttct tccataacct ttttaccgtt cagatattta cggggcgagg cgaatgcgtc
     9841 ttgcctggta taccgccgag taagcctggg tgggattcac accattactg atttcactga
     9901 ccacatgacg ctgatcagcc agtgtcagcg tgtaaacctg tttctggatg aggtcacggg
     9961 ctttgtcatg ctcatatccg gcagcaacaa gggtgtcgag aagtcgtttg tctgttttgt
    10021 tcagtttatg gcggggtgat ttgctcacat cgttctccgt tttttcatga tccaacagtt
    10081 tatatctcat cctgatttgc tccggtgttc tgtttcgacg ttctcaatgc ctgcctcccc
    10141 atagtgtgat ccttattgca aaaatgaaaa tccatgctct tgacatggac aatactgagt
    10201 agtaggtttt actgtacata aaaccagtgg ttatatgtac agtatattgt tttaatttat
    10261 tgttttaatt atcaaagagg aatttatggc taaagaacta agtggatatg gaccaactgc
    10321 tggtgagtct atgggggaac aggggcgaat ctgaatcagc aaggtggaaa taataatagt
    10381 aatagtgggg ttcactgggg tggtggctct gggcatggta ataatggtgg tcaggaaatt
    10441 caaaattcat ctggttctac atcaactgtg atgaaaaccg gtgagtcata tctgactccg
    10501 tggggtgatg ttgtcattaa taatgatggc cttccggtga tgaatggtat tgttatgaca
    10561 gaagagaatt caactctggt tgataatcca tttggagggg tctccagagt tcttaattct
    10621 ttaattagtg atatgccatc actttttgca gaaagctcag gaaataacaa caataacact
    10681 gcttcagtta atactgcacc aacgaatgct caggtcagtg atatggataa gagttcgaag
    10741 gtggtgggta atgtaattaa tgaaaaacaa aagcagaaga ataaaattgc tacacagatt
    10801 agtgaaaaac agaagaaaat cgaggagatg aaaaaagtat tcaagcatca ttcttatcat
    10861 ggtatcactg atcttgagag ggatgttgat gagttgcaaa aaatcaaatc agcttgatgc
    10921 agatattagc aaacttaatt cttataaaaa tacattgcaa agtaaaattg gtgatgtgaa
    10981 taaacagaag gaagctgaag aaaaggctcg tgaaaatgca gaggtcgctg aacatgaaac
    11041 tcttaatgaa gagaaacagg cggtggcgga agcagagaag cgtttagctg aggtgaaagc
    11101 tgaactggcg aaggctgaaa gtgatgtaca gagtaagcaa gctactgttt ccagagttgc
    11161 aggagagctt gaaaacgctc agaagagtgt tgacgtgaag gtcaccggat ttccgggatg
    11221 gcgtgacgtt cagaaaaaac tacagcgtca gcttgaagct aagcaggctg agtactccgc
    11281 agttgaaaat gaacttaaga atgctgttag tttcagggat ggtaaggccg ctgaggttaa
    11341 agaggcagag cagaaattaa aagaagctca ggatgctctt gaaaagagcc agataaaaga
    11401 tgctgttgat actatggttg gattttatca atatataacc gaacaatatg gagaaaaata
    11461 cgcaaaattg ctcagattta gcggaaaagt ctaaaggaaa gaaaattcaa ggtgttgatg
    11521 aagctctggc agcatttgaa aagtatgtaa gaatgtactg gataagaaat tcagtaaggt
    11581 tgatcgggat gctattttta atgcgttaga gtctgttaat tatgatgagt tatctaagaa
    11641 tttaacgaag atatcaaagt cgcttaaaat caccagtcga gtatcttttc tatatgatgt
    11701 tggttctgat tttaaaatgc aatagaaact ggtaactggc gacctttgtt tgtcacgctg
    11761 gagaaaagtg cggtcgatgt aggtgtggca aaaattgtgg cattaatgtt tagttttatt
    11821 gttggtgtgc ctcttggttt ctggggattg caattgtcac aggtattgtt tcttcttaca
    11881 taggggatga tgagttgagc aaacttaatg agttgttagg gatttaattt ctctttagag
    11941 tgtaatatat gaaacttggc aatcctactg ggattgccat taataaataa aacaatgcag
    12001 ataacttgca taagtaccag tggttctttt ttaactttgt attttctttg atatatctag
    12061 atatggttaa tttagagtat ggatatagca atacatgtat tgatgatata aatataaata
    12121 acaaaggagt gttatcattg tcgttaaata ttaaaataag aatccatggt ataagtgatt
    12181 caggtaaatt atgtaggtag tattttaaat gcataaggac ttttaccaat ggctgctgcc
    12241 agtggcgctt tgtggtgtct tttccgggtt ggacccaaga gaacagttac cggagaaggc
    12301 gcagcagtcg ggctgaacgg ggggttcctg catacagccc agcccggagc gaactgccta
    12361 agtcgccccg agataccaga agacgtgagc tatgagaaag cgccacgtgt cccgtaaggg
    12421 agaaaggcgg aaaaggtatc cggtaatcga tatggcggga acgtgggggc gcaggaggga
    12481 gcgattcacc ggaaacggtg atgatcttta agccacgtcg ggtttcgcca ccgctggctt
    12541 gcagtgtctg ttttgtgaag tccgtcaggg gggcggagcc tatggaaaaa gcgcctccgg
    12601 cgcggccttt cccggggttt cgttttttgc gcacatgttc tttcctggtt tatccccggg
    12661 ttctgtggat aacagggtta gcgtttttga gaaaggagag tccgctcgcc gcagccgaac
    12721 gaccgagcgt agcgagtcag tgagcgagga agcggaagag cgcctgaact ggcattttct
    12781 ccttacgcat ctgtgcggca tttcacaccg gacatggcgc acttttcata caatccgcac
    12841 tgatgccgaa tggttaagcc agtatacact ccgctatcgc tacgtgactg ggtcagggct
    12901 tcgccccgac accccaaagg tcaacggcct gacgcgccct ggcgggcttg tctgctcccg
    12961 gcatccgctc acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gcggttttca
    13021 ccgtcatccc cgaagcgtgc gaggcaactg cggtaaagct catcggcgtg gtcgtgaagc
    13081 gattcacaga tgtctgcctg ttcatccgcg tccagttcgt tgagcttctc cagcagcgtt
    13141 aatgtctggc ctctgatgaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact
    13201 gatgcctccg tgtaaggggg ggttctgttc aggggggtaa tgataccgat gaaacgcgag
    13261 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgctgtgag
    13321 ggtaaacaac tggcggtatg gatgcggcgg gtctgcctgg gtgtcaggtc ttccgacact
    13381 ggcacagccg ctgttgcgtc agttcgcttg gtcgggaata accttaacca gacagcgagg
    13441 aaggtgaaca gcgggcagtg gtcttccggt gaccgggttc aggtggtggc cgcactgatg
    13501 gccatcggac gtgaactgag tgagctgagg gatgaggtcc gtagggaaca gggggcgcgg
    13561 gatgatagtt aagtttcatg ccaggggaaa aggtggtggc agtggtccgg ttgattacct
    13621 gctgggaagg gagcgtaacc gggaaggggc aacggtgctt caggggaatc cggaagaggt
    13681 ccgggaactc atcgatgcca cgccatttgc gaagaaatac acgtcaggtg ttctgtcgtt
    13741 cgcggagaag gagctgccgc ctggagggcg tgaaaaagtg atggcgagct ttgagcgtgt
    13801 actgatgccc ggtctcgaga aaaatcagta cagcatcctg tgggtggagc accaggacaa
    13861 gggacggctt gagctgaatt ttgtcattcc gaacatggag ctacagaccg gaaaacgcct
    13921 ccagccgtac tacgaccggg cagacaggcc gagaattgat gcctggcaga cgctggtgaa
    13981 tcaccattac ggtctgcatg accccaacgc cccggagaac cgccggacgc ttgtcacccc
    14041 gataacctgc cggaaaacga aacaggaggc cgcagaagcg attacgaggg actggaggcg
    14101 ctttaccatg ccggagagat aaaaggccgt caggatgtga ttcaggcgct cacagaggcg
    14161 gggcttgaag tgacgagttc aggaagctcg aagaatctgt aaacagggaa ctgacctcaa
    14221 acgggcgaaa tcagaacgct atcagcgcac acacagcagc ggtgaaagaa caactggaga
    14281 cgctgaacag tgccgtgaac gcgcagttca gcgccacaga gaacgaactg gcccggcggc
    14341 aggaagagct gctctggaag ctggtcaaag ggcggatact gtacccgtcg ctgacggcgc
    14401 tttgcgtcac gggcgggatt tttctggcgt catgggggct gattcagtgg caggaaagcc
    14461 ggattgcggc gaacatcctg gctatcaggg accaggaaga gacgctggcg aaactccggg
    14521 aaaaaacatg gggtgtgacg tatctcgagg gcaggaacgg gaaatttctg gtgttgccgt
    14581 ccgggggtga agggggaaaa caactggacg gtggagaaga agaacgcagt aagactggtc
    14641 agggagtaag tgagtatgac agagctggaa atgcacttac tgaacgcctt agagcaattg
    14701 cagcaggact atacgcagcg gctgaaagac tgggagagcg ccttcacgga tttgcagcat
    14761 atgttcgagc tgacacgaca ggagaacggg caactgaggg aacagtgcga cgacttgagt
    14821 cagcaggtgc agagattagc cgggcaggtg gaccacttga gtcggttatt tatcacgaac
    14881 agcagataag ggatgaacag gaaaggcagg cacgcctgga acgggagcgt gcgcagtcct
    14941 ggcatgaaga gcaggaacgg gaagcacggg tatggcgtgg cccgtcaata gggatgtgac
    15001 ggtgagttgt tccgggaggt tgccagatgg taccggaacg cgttaaaaac gcatccgggc
    15061 gcgattctgg cgtgtcgttt ttgttgctgt gttttgcctc tttgccagat cgcgctcatt
    15121 cctcagcctc atcccgcgat gtctgcgtat gtcatcaact gaaaagatgg aaaaaaaaca
    15181 gtgtcatgag gtttacgcag gcaaaaccag cgttattcac atggctgaat agctatgcat
    15241 acggttaaaa tttatcaggt gcgatcgcgg cagcttttcg ggtggtttgt tgccattttt
    15301 acctgtcagc agccgtgatc gcgctgaacg catttcagcg gtgcgtacaa ttaagggatt
    15361 atggtaaatc ccttactgtc tgccctcgta tccatcgaga taaaccgcag aaatggtgtc
    15421 tgccattagt ttcatttttt cggcgaaggc atatcgctgt ttgtgtattt tctgccagca
    15481 aaggctgttg tgctccggag tgagtgaaaa aatttttctg agtggaattc ttttacacgt
    15541 tgctgtgccg tttctgctga tactccggct gctttgtctt tcatccccat atcccggaac
    15601 tgctgaaaga acgggagcat catacgtatg gtgtctgctt tctgggtgta gaagttagga
    15661 tcaaatccat catctgaagc ataaacataa tgccgggtat gtcgttccag gctggtcttt
    15721 ggtgtgcatc ctgtaagagc tattgttgct gttatcagag gtatcagaag cagcctggca
    15781 gcggcagatc tcataaacat ctccttgcgt ttttaatctt gatactcagg gagttatatc
    15841 tccgaagagt atctcttatc atacgtcggt ttactcccaa tgtgatttga tggtcaatga
    15901 gtcaggaata atcccgtgaa aaaatgtgtg ccagtcctgc tttttagtgt tctttctgct
    15961 tcggttgcca gtcatgcgga cagcaatgtt tttcaacaaa aggccgagaa gacaaaaaac
    16021 ggcattgttg cggatgttaa tgccagacag aaaatattca gtaacagact gaagatacaa
    16081 cttcagatcg atgagttgag agcagagctg gctggcacca gagatgccca aaaaagagag
    16141 gatttgcgta aaaactggag tctctggagc ggcaaaagaa atccttacgc tgatgggggc
    16201 gtattttccc cggtttcttc cataaccttt ttaccgttca gatatttacg ggcgaggcga
    16261 atgcgtcttg cctggtatac cgccgagtaa gcctgggtgg gattcacacc attactgatt
    16321 tcactgacca catgacgctg atcagccagt gtcagcgtgt aaacctgttt ctggatgagg
    16381 tcacgggctt tgtcatgctc atatccggca gcaacaaggg tgtcgagaag tcgtttgtct
    16441 gttttgttca gtttatggcg gggtgatttg ctcacatcgt tctccgtttt ttcatgatcc
    16501 aacagtttta tatctcatcc tgatttgctc cggtgttctg tttcgacgtt ctcaatgcct
    16561 gcctccccat agtgtgatcc ttattgcaaa aatgaaaatc catgctcttg acatggacaa
    16621 tactgagtag taggttttac tgtacataaa accagtggtt atatgtacag tatattgttt
    16681 ttaatttatt gttttaatta tcaaagagga atttatggct aaaagaacta agtggatatg
    16741 gaccaactgc tggtgagtct atggggggaa caggggcgaa tctgaatcag caaggtggaa
    16801 ataataatag taatagtggg gttcactggg gtggtggctc tgggcatggt aataatggtg
    16861 gtcagggaaa ttcaaattca tctggttcta catcaactgt gatgaaaacc ggtgagtcat
    16921 atctgactcc gtggggtgat gttgtcatta ataatgatgg ccttccggtg atgaatggta
    16981 ttgttatgac agaagagaat tcaactctgg ttgataatcc atttggaggg gtctccagag
    17041 ttcttaattc tttaattagt gatatgccat cacttttgca gaaagctcag gaaataacaa
    17101 caataacact gcttcagtta atactgcacc aacgaatgct caggtcagtg atatggataa
    17161 gagttcgaag gtggtgggta atgtaattaa tgaaaaacaa aagcagaaga ataaaattgc
    17221 tacacagatt agtgaaaaac agaagaaaat cgaggagatg aaaaaagtat tcaagcatca
    17281 ttcttatcat ggtatcactg atcttgagag ggatgttgat gagttgcaaa aaaaatcaaa
    17341 tcagcttgat gcagatatta gcaaacttaa ttcttataaa aatacattgc aaagtaaaat
    17401 tggtgatgtg aataaacaga aggaagctga agaaaaggct cgtgaaaatg cagaggtcgc
    17461 tgaacatgaa actcttaatg aagagaaaca ggcggtggcg gaagcagaga agcgtttagc
    17521 tgaggtgaaa gctgaactgg cgaaggctga aagtgatgta cagagtaagc aagctactgt
    17581 ttccagagtt gcaggagagc ttgaaaacgc tcagaagagt gttgacgtga aggtcaccgg
    17641 atttccggga tggcgtgacg ttcagaaaaa actacagcgt cagcttgaag ctaagcaggc
    17701 tgagtactcc gcagttgaaa atgaacttaa gaatgctgtt agtttcaggg atggtaaggc
    17761 cgctgaggtt aaagaggcag agcagaaatt aaaagaagct caggatgctc ttgaaaagag
    17821 ccagataaaa gatgctgttg atactatggt tggattttat caatatataa ccgaacaata
    17881 tggagaaaaa tacgcaaaaa ttgctcagga tttagcggaa aagtctaaag gaaagaaaat
    17941 tcaaggtgtt gatgaagctc tggcagcatt tgaaaagtat aagaatgtac tggataagaa
    18001 attcagtaag gttgatcggg atgctatttt taatgcgtta gagtctgtta attatgatga
    18061 gttatctaag aatttaacga agatatcaaa gtcgcttaaa atcaccagtc gagtatcttt
    18121 tctatatgat gttggttctg attttaaaaa tgcaatagaa actggtaact ggcgaccttt
    18181 gtttgtcacg ctggagaaaa gtgcggtcga tgtaggtgtg gcaaaaattg tggcattaat
    18241 gtttagtttt attgttggtg tgcctcttgg tttctggggg attgcaattg tcacaggtat
    18301 tgtttcttct tacatagggg atgatgagtt gagcaaactt aatgagttgt tagggattta
    18361 atttctcttt agagtgtaat atatgaaact tggcaatcct actgggattg ccattaataa
    18421 ataaaacaat gcagataact tgcataagta ccagggttct tttttaactt tgtattttct
    18481 ttgatatatc tagatatggt taatttagag tatggatata gcaatacatg tattgatgat
    18541 ataaatataa ataacaaagg agtgttatca ttgtcgttaa atattaaaat aagaatccat
    18601 ggtataagtg attcaggtaa attatgtagg tagtatttta aatgcataag tatttttact
    18661 tttgttggaa agaaatatga aaataataat ttttctaatt acaatattat tcttgtcagg
    18721 atgtcaagca aactatattc gtgatgtaca aggaggaacc gttgcaccat cttcgtcttc
    18781 tgaactgacg gggatcgcgg ttcagtagaa aagatcaaag gatcttcttg agatcctttt
    18841 tttctgcgcg taatctgctg cctgtaaacg aaaaaaccgc ctggggaggc ggtttagtcg
    18901 aaggttaagt cagttgggga ctgcttaacc gggtaactgg ctttagtgga gcacagatac
    18961 caaatgctgt ccttccagtg tagccgctat aaggcttcaa cttcaagagt tctgcgtatc
    19021 taaatccact atccagttac caatggctgc tgccagtggc gttaagtcgt gtcttaccgg
    19081 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    19141 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc aacagcgtga
    19201 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    19261 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    19321 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    19381 gggcggagcc tatggaaaaa cgccagctgc gtggcctttt ccgggttctt gttttttgct
    19441 cacatgtttc ttttggtgtt atcccctgat tctgtggata acagcatttc gtttttgagt
    19501 gagtagatac cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag
    19561 cggaaaagcg gctggacgtg cattttctcc ttacgcatct gtgcggcatt tcacacccgg
    19621 tatggcgcac ttttcatacc atccgcactg atgccgcatg gttaagccag tatacactcc
    19681 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgctaaaac ccgctgacgc
    19741 gccctgacgg gcttgtctgc tcccggcatc cgctcacagg ccagctgtga ccgtctccgg
    19801 gagctgcatg tgtcagaggt tttcaccgtc atccccgaag cgtgcgaggc agctgcggta
    19861 aagctcgtca gcgtggtcgt gaagcgcttc gcagatgtca gcctggtcgt cggcgtccag
    19921 ttcattcagt ttttccagca gggtcagcgt ctggcttctg atgaagcggg ccatgttaag
    19981 ggcggttttt tcctgtttag tcacagttct gtttctccat gtgtggggag tctgtcggag
    20041 gtttttaatg ataccggtga aacgcgaaag gatgctcacg atacgggtca cggatgagga
    20101 acatgcgcgg ctgctggcac gttgtgaggg aaaacaactt gcgtcatgga tgcgtaaggt
    20161 ctgcctgggg gcaccgccat cgaaaacgtc aggtcttccg acactggcac cgccgctgtt
    20221 gcgccagttc gcttcggtcg gcaataacct gaatcagata gcccggaaga taaacagcgg
    20281 acagtggtcg gggcatgacc gggttcatgt ggtggctgca ctgatggcca tcggacgtga
    20341 actgagtgag ctgagggatg aggtccgtaa acagggagaa cggg
//
