LOCUS       CP103360                6784 bp    DNA     circular BCT 31-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-612 plasmid unnamed4, complete
            sequence.
ACCESSION   CP103360
VERSION     CP103360.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323488
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6784)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 146x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 22:15:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,528
            CDSs (total)                      :: 4,435
            Genes (coding)                    :: 2,373
            CDSs (with protein)               :: 2,373
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 2,062
            CDSs (without protein)            :: 2,062
            Pseudo Genes (ambiguous residues) :: 0 of 2,062
            Pseudo Genes (frameshifted)       :: 1,976 of 2,062
            Pseudo Genes (incomplete)         :: 82 of 2,062
            Pseudo Genes (internal stop)      :: 114 of 2,062
            Pseudo Genes (multiple problems)  :: 105 of 2,062
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            NCBI analysis of this genome has determined that >30% of the
            predicted CDS features contain frameshifts, which is well above the
            usually observed value of <10%.
FEATURES             Location/Qualifiers
     source          1..6784
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-612"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="2016-11-01"
     gene            1..330
                     /locus_tag="NXW60_22585"
     CDS             1..330
                     /locus_tag="NXW60_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVV57597.1"
                     /translation="MRRLPNNQMEFNIGLGVKAHSLDVVADIIGTMRMQKIIEITENVQ
                     PNEKERIEQLDKELHILTIEEKIVLGLNPYNLNEEDANTIRQSVIDKVIRLYAPILKKF
                     NTENE"
                     /name="hypothetical protein"
     gene            323..583
                     /locus_tag="NXW60_22590"
     CDS             323..583
                     /locus_tag="NXW60_22590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVV57598.1"
                     /translation="MNKEMSLDVALDIIGTLRMMKIDEISEEKDENKKELLYKELDILT
                     TEEEVANGLLQFEVSEKVRLSIMDKIENYYAPKLKAYYTAL"
                     /name="hypothetical protein"
     gene            complement(736..1242)
                     /locus_tag="NXW60_22595"
     CDS             complement(736..1242)
                     /locus_tag="NXW60_22595"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraG/TraD/VirD4 family protein"
                     /protein_id="UVV57601.1"
                     /translation="MAQLEDGYGREKADVLFASCNSHFYGRVASSKTAEILSKQFGKAD
                     KVYITSNEGRNTRKFGKTFGRSETIQERDVIKPSEFLNLQVGEFAGLAVESNKPTFRTR
                     FKQAQRPQPAELKKPDFSGSVSDYYKRVRYDINKMLEIGGTAGEYDRDTMKLNETKRNF
                     FGVEY"
                     /name="TraG/TraD/VirD4 family protein"
     gene            complement(1247..2401)
                     /locus_tag="NXW60_22600"
     CDS             complement(1247..2401)
                     /locus_tag="NXW60_22600"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="UVV57599.1"
                     /translation="MKEGQLKSSNRSGCLMYLALIYAFPMLAALAVLPLGVVYLCFRAG
                     EIGNGLFVLAVEAGAVYLFIKYKRKGKKEEAPKNPNTAILRTEAGGVQLGNPFRGVFVL
                     GAAGSGKSESIAVPLLSEFVRMGFAGVVYDFKFPTLAKDVQSFVNAKGSVLRHFYLDFN
                     NPAASYRVNPLNPRYLPNTSYAREYAQAIISNLMKESIKKPDFWTRSATDLLTACIWYL
                     KEERPDICDLPHVLAMITSNDTALLKTLQKNVQTAQMTVSIYNAMERGADSQVSGVIGT
                     LQGAIAQINTPELMFLFSGDDFSLDVNDPQNPIILTVGSYPTLTQTFAPLCSLVITVAT
                     KLMNQPDKHPSFVLLDEAPTVFIPNIEVLPNTGRSNKIATVIMS"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     gene            complement(2406..3017)
                     /locus_tag="NXW60_22605"
     CDS             complement(2406..3017)
                     /locus_tag="NXW60_22605"
                     /inference="COORDINATES: protein motif:HMM:NF040155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5712 family protein"
                     /protein_id="UVV57602.1"
                     /translation="MDNNRKGLKAKEAKFYVITVAPSREELRKMGATKEEQAAAMKEYI
                     RTEVMPKYAEGFNKGLKADDLEFYGKIHFERHEKDGEDLHAHIIVSHKTKNNGKSISPM
                     TNHTGKKNTGAAQGGFNRKEWYSSCEKAFDKRFKNERDIKESFEYKNAMKNGTPKEMQE
                     QINRAIQQERQREQIRQQQEQRVTQAVKTEKKDNKVKPKL"
                     /name="DUF5712 family protein"
     gene            complement(3326..3736)
                     /locus_tag="NXW60_22610"
     CDS             complement(3326..3736)
                     /locus_tag="NXW60_22610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="clindamycin resistance transfer factor btgA"
                     /protein_id="UVV57600.1"
                     /translation="MEEKRPNTPRNSTVAVSPEIGKKLERFCASCGITKKDFISLSLDY
                     FQRYGINPAKHESPAKEMEKLIKKNDQVIGFIRKQEQDILRPMLEAITTTEARIKADFD
                     SLAKYDLAKYEKICEFVDASIKACDDIADEKK"
                     /name="clindamycin resistance transfer factor btgA"
     gene            4119..4313
                     /locus_tag="NXW60_22615"
     CDS             4119..4313
                     /locus_tag="NXW60_22615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="UVV57591.1"
                     /translation="MKKGDKVLISPDLTKLPNWISGIVIEVENNPFVGIVISAETEDKN
                     VFFGQEDLFKPQTEEVCLP"
                     /name="transcriptional regulator"
     gene            4301..4582
                     /locus_tag="NXW60_22620"
     CDS             4301..4582
                     /locus_tag="NXW60_22620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence protein"
                     /protein_id="UVV57592.1"
                     /translation="MFAISFDMSISDLEKHYGKPYNRAYFEIKEVLKRNSFDWIQGSTY
                     LTQSEDLGNLFRAIEALKKIEWFRKSVRDIRGYKVENWSNFTDIVKDI"
                     /name="virulence protein"
     gene            complement(4637..4762)
                     /locus_tag="NXW60_22625"
     CDS             complement(4637..4762)
                     /locus_tag="NXW60_22625"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVV57593.1"
                     /translation="MEKNDFLHNGKAKQEAKRSGEKDEIPRCVKKGFFMYITVNC"
                     /name="hypothetical protein"
     gene            5077..6051
                     /locus_tag="NXW60_22630"
     CDS             5077..6051
                     /locus_tag="NXW60_22630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UVV57594.1"
                     /translation="MNINNKDVIQSYIMTTAKYDFSADEKRILLRLVETWQYLLEGKEL
                     KGKIDRDLFGGYILEFPISYFVPDNQTNYKRIKDALRSLNDKKFEYEDSNLWEIIRIIE
                     RPQIRKREKVIFELNEKIVECFLNFTKGYRKFELETALSFSSVYAVRFYELMSGQTTPL
                     TFTFTKLKEMFQLTDKYPRTPDFIKRVIEPAKRELDEKSPFSFEYRYKEPDMKDGKKFY
                     KIMFYPIAIPRNRDEHVERKDLLSQKVNLSAFLDKVDREYLAKLGFSDRQIKNNYDLIL
                     SAKKELDLVYELSLLIGKVRGKKNPQGYVISTLRGKLKDKKIK"
                     /name="replication initiation protein"
     gene            6054..6221
                     /locus_tag="NXW60_22635"
     CDS             6054..6221
                     /locus_tag="NXW60_22635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVV57595.1"
                     /translation="MEELKVKVVEVSQEKEQPQGNDYIRVTVEIDGRKIAVWTTESNIN
                     ALRFDIDKKR"
                     /name="hypothetical protein"
     gene            6300..6770
                     /locus_tag="NXW60_22640"
     CDS             6300..6770
                     /locus_tag="NXW60_22640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVV57596.1"
                     /translation="MSDIQTSTIRVPKNVLEDIKIYCRKAGQPVGEWVEKTWSFLQKND
                     FDIYDTEATPFLPVPAEVEKERSQVDALCKLMSEFILSQKQVQLPAPEIIAKAAEEKAK
                     AESKVQEQAQELQRLRDENKALRERYEKAHKELCRVRDEQKTIGKIKVNTNF"
                     /name="hypothetical protein"
     Typing          complement(2536..3074)
                     /name="MOBB"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.222"
                     /coverage="84.0"
                     /evalue="1.21e-114"
ORIGIN
        1 atgagaagac ttccaaacaa tcaaatggaa tttaacatag gtttaggtgt taaagcacat
       61 tctctcgatg tggttgccga cattatcggc acaatgagaa tgcaaaaaat tattgaaatc
      121 actgaaaacg tacagccaaa cgagaaagag agaatagaac aacttgataa agaactccat
      181 atacttacta tagaggaaaa aatcgtactt ggactcaacc catacaattt gaacgaagaa
      241 gatgcaaaca cgatacgtca atcagtaata gacaaagtaa tacgtctata tgctccaata
      301 ttaaagaaat tcaacacaga aaatgaataa agaaatgagt ttggatgttg cgctcgacat
      361 tatcgggaca ctccgcatga tgaaaataga tgaaatatca gaagagaagg atgaaaacaa
      421 gaaagaattg ttatacaaag aactggatat acttaccaca gaggaagaag tagcaaatgg
      481 attactacag tttgaagttt ctgaaaaagt acgcttatct atcatggata agatagaaaa
      541 ctattatgca cccaaattga aagcatacta tacagcacta tgagataggg ataaaaaaca
      601 aaaagatagc tatatcgaac cacaaaccac agaaatagta ttgccgggtg ttacaaaatg
      661 atattcagta cttggctgat gggggcggtt cttcccccat tcagaccaaa gcaactatca
      721 aaatgataaa ggttttcaat actcaacccc aaagaaattt ctctttgttt cgttcagctt
      781 catagtgtcc ctgtcgtact ctccggcagt tcctccaatt tcaagcattt tatttatatc
      841 atatctcacc cgcttgtaat aatcgctcac agagccggaa aagtcgggct tcttcaattc
      901 tgcgggctgc ggtctttgtg cctgtttaaa tcgggttctg aacgtgggct tattgctttc
      961 aactgccaac cctgcgaact ctccaacttg caagtttaaa aactctgacg gcttgataac
     1021 atcacgttcc tgtattgttt cagacctacc aaaagttttt ccaaacttcc tcgtattgcg
     1081 tccctcgttg gatgttatat ataccttatc ggcttttccg aattgtttag aaaggatttc
     1141 agccgttttg ctgcttgcta ccctgccata gaaatgggaa ttgcaggaag caaacaaaac
     1201 atcggctttt tccctaccgt aaccgtcctc taactgtgca atatccttaa gacattataa
     1261 ccgttgctat cttgttgctc ctgcctgtat tcggcaaaac ttcgatattc ggtataaata
     1321 cggtcggtgc ttcatccaaa agaacaaaag acggatgttt gtccggctgg ttcatcagct
     1381 tggtagcaac cgttatcact aaagagcaca atggggcaaa agtctgtgtt aatgtcgggt
     1441 aacttccgac tgtcaaaata atagggtttt gcgggtcgtt cacatcgagt gagaaatcat
     1501 cgcccgaaaa caagaacata agttcgggag tgtttatctg tgctattgct ccttgtaatg
     1561 tgccaataac cccgcttact tggctgtctg cccccctctc cattgcattg taaatactaa
     1621 cggtcatttg tgccgtttgt acgttcttct gcaaagtttt aagtagtgcg gtatcattac
     1681 tcgttatcat ggctaaaacg tgcggtaaat cgcaaatatc ggggcgttcc tctttcagat
     1741 accaaataca ggcggtcaag aggtcggttg cgcttctcgt ccaaaagtcc ggcttcttga
     1801 tactctcttt cattagattg cttattattg cctgtgcgta ctcacgtgca taagacgtat
     1861 taggcaaata tcgtggattt aaagggttta ccctgtaaga tgcagccgga ttattgaaat
     1921 ccaaataaaa gtgtcttaaa acgcttcctt tggcgtttac aaaggattgt acatcttttg
     1981 ccaatgtcgg aaatttgaaa tcatatacca cacccgcaaa acccatgcgg acaaactcgg
     2041 aaagcaaagg cactgcaata ctttcacttt tcccgcttcc ggctgctcct aatacaaaaa
     2101 cccctctaaa tgggtttccc aactgcaccc ctccggcttc tgtccgcaaa attgccgtat
     2161 ttgggttctt gggggcttcc tctttttttc ccttacgttt gtatttgata aacaaataga
     2221 ctgctccggc ttccaccgca agcacaaaca aaccgtttcc gatttctccg gcacgaaaac
     2281 acaaatacac cacacccaaa ggcaaaaccg ccaaagctgc aagcatgggg aaagcgtaaa
     2341 tcaatgccaa atacatcaaa caaccgctac ggttgctact ttttaattgt ccctctttca
     2401 tgattttata attttggttt tactttatta tccttttttt ctgtcttgac agcttgtgtt
     2461 accctctgtt cctgttgctg tcgtatctgc tccctctgcc gttcctgctg aatggctctg
     2521 tttatctgtt cctgcatttc ttttggtgtg ccgttcttca tggcgttctt gtattcaaaa
     2581 ctttccttta tatcacgttc atttttaaaa cgtttatcaa aagccttttc acatgaagaa
     2641 taccactctt tgcggttaaa tcccccttgt gctgcacctg tattcttttt ccctgtgtga
     2701 tttgtcatgg ggctgattga tttaccgtta ttcttggttt tatggcttac gataatatgg
     2761 gcgtgcaaat cttctccgtc tttttcgtgc cgttcaaaat ggattttacc gtaaaactcc
     2821 aaatcatcag ctttcaaccc tttattaaac ccctctgcat atttcggcat aacttcggta
     2881 cggatatact ctttcatggc tgctgcctgt tcctctttcg tggctcccat tttacggagt
     2941 tcctcacgtg acggcgcaac agtgataaca taaaactttg cttctttggc tttcaatccc
     3001 tttcggttgt tgtctatcct gttggttact tctttggggc ttatcctgtt ctgttcccga
     3061 ttaaagaacg gttcagtgcg ttaaccctgc ttgcagccgt tctgcatctt catgctgcaa
     3121 ataggaaaca acatcagagc agctgccggt gttggcataa actttaccgc ctccctgtac
     3181 ttttactatc attatccaaa aagtctatcc attaaacccg cttttccttt ctcgtccaga
     3241 tgttgtacaa gcattttaat aatcctaatc acattattgt tatgcttcac gcttgtatcg
     3301 tacatcctat tcatttcagt tataatcatt ttttttcatc tgcaatatcg tcacatgcct
     3361 ttatggaagc atctacaaac tcacagattt tttcatattt ggctaaatca tatttggcta
     3421 aactgtcaaa atctgccttt atcctcgctt cggttgtggt tatggcttca agcatggggc
     3481 gcaaaatgtc ctgctcctgc ttacggataa acccgattac ttggtcgttc ttcttgatta
     3541 gtttttccat ttctttggcg gggctttcat gcttagccgg atttatcccg taccgttgaa
     3601 aatagtctaa cgaaagggaa ataaaatctt tcttcgtgat accacatgaa gcacaaaaac
     3661 gctctaattt cttgccgatt tcgggagaaa cggcaacagt agaattgcga ggagtattag
     3721 gtcttttttc ttccatatta taacggttta tatatcatta agtactgata caaaagtagt
     3781 tatttattac aatatatcaa tactatatat aaaaaatcat tcgatttttt ataaacggct
     3841 gattttcagc cgtaaccccg tagggcaaga ggaaaaaagg caactctgtg gcgtttcctc
     3901 ttgctattcc tattcgactg cctaagcagc cgacggaata cccctcccct gcggtgcggg
     3961 cgggcgtgag ctgcgccaat gcacaagggg gcggggggtt atgcagtcgg atgtgtggcg
     4021 ggctaatagg aatagttccg actgcgcata atttaacagg tacaaacagc taagtaaatg
     4081 gggaaatctg tatctttgaa aacaaaaata atagctatat gaaaaaagga gataaagttt
     4141 taatttctcc cgaccttacc aaattaccga attggataag cgggatagtg atagaggttg
     4201 agaacaaccc atttgttggt atcgtcatct ctgccgaaac agaagataag aatgtgtttt
     4261 tcggacagga agatttgttt aaacctcaaa cagaagaagt atgtttgcca taagttttga
     4321 tatgtctatt tcagaccttg aaaagcacta cggaaaaccg tataatcgtg cttactttga
     4381 aattaaggaa gtcttaaaga gaaatagttt cgattggata caaggaagta cataccttac
     4441 acaaagcgaa gatttaggca atctgttcag agccattgaa gcactaaaga aaatagaatg
     4501 gttcagaaag tcagtacgtg acattagagg ttacaaagta gaaaactggt ccaactttac
     4561 agatattgta aaagatatat aagcaaaaat ctgtataaca aatagttata tccaaaagac
     4621 gaccaaatca cgaacgtcaa caatttacag taatatacat aaaaaaacct tttttaacgc
     4681 agcggggaat ttcgtccttt tccccgctgc gcttggcttc ttgctttgct tttccattat
     4741 gcaaaaagtc gttcttttcc atattgtgtt gatattaagc cacttatatt ctaaaatcaa
     4801 tactttgtgt taaatagcat aacttgttta tgcttatcac tttttaaaag cacgttatat
     4861 cttgttataa atcaaccgat tatattcatt ttatttttca aaagacgact ttttgcatag
     4921 ttaaagacga ctttttgcat agtttttaag ggggtcaaag acgacttttt gcatagtttt
     4981 ctaaaataaa atagactttg ttttctgtat gtcgttttta tatgtatatt tgctgcgaaa
     5041 caactatgca agttgtcgtt tttaaaacgt aattatatga atataaacaa taaagacgtt
     5101 attcagtcgt atattatgac taccgcaaaa tatgactttt ctgcggatga aaagcgtatt
     5161 ctcttacgct tggttgaaac atggcaatac ttgttagagg gcaaagaact aaagggaaaa
     5221 atagatagag atttatttgg cggatatatc ttagagtttc ctataagcta ctttgttcca
     5281 gataatcaaa cgaactacaa acgtataaaa gacgctttgc gttctctcaa tgataagaaa
     5341 tttgaatatg aagattcaaa cctatgggaa attatacgca ttatagaaag accgcaaatt
     5401 cggaaacgag aaaaagttat ttttgagtta aacgagaaga ttgtagagtg cttccttaac
     5461 tttaccaaag gttacagaaa gtttgagtta gaaacggctc tatccttttc atctgtctat
     5521 gctgtacgct tctatgagct aatgagtggg caaacaacac ctttaacctt tacttttact
     5581 aagctgaaag aaatgtttca acttacagac aaatacccac gtacacccga tttcataaaa
     5641 cgtgtcattg aacctgcaaa aagggaatta gacgaaaaaa gcccattttc atttgaatac
     5701 agatacaaag aaccggacat gaaagacgga aagaaattct ataaaatcat gttctatcct
     5761 attgcaattc cacgcaacag ggatgaacat gtagaacgaa aagacctatt atcccaaaaa
     5821 gttaatttat ctgcgttctt ggataaggtg gatagggaat accttgcaaa gttagggttc
     5881 tctgataggc aaataaaaaa caactatgac ttaatattgt cagctaaaaa agagttagac
     5941 cttgtgtatg aactgtcctt acttatcggg aaagttagag gaaagaagaa cccacaagga
     6001 tatgtaataa gtaccctaag aggaaaacta aaggacaaaa aaataaaata gacatggaag
     6061 aattgaaagt taaagtagtg gaagtttcac aggaaaagga acaaccacaa ggtaacgatt
     6121 atattcgtgt taccgttgaa attgacggac gaaaaatagc cgtatggact accgaaagca
     6181 acatcaatgc tctacgcttt gatattgaca aaaagagata aatatatttt ttgtagattt
     6241 tgtagaaagc taaatatatt ctttatattt ttgcatataa ctaattaaca tatagatata
     6301 tgagcgatat acaaacaagt accattagag tgcctaaaaa cgtgcttgaa gatataaaaa
     6361 tatattgccg taaagcgggg caacctgttg gggaatgggt agaaaagaca tggagtttct
     6421 tgcaaaagaa cgatttcgat atttacgata ctgaagctac accttttctt cctgttccgg
     6481 ctgaagtaga aaaagaacgt agtcaagtgg atgcgctttg caagctgatg agtgaattta
     6541 tattatcaca aaagcaagtg cagctacctg cccccgaaat cattgccaaa gctgcggaag
     6601 aaaaggctaa agccgaaagc aaggtacagg aacaggcaca agaactgcaa aggcttcggg
     6661 atgaaaacaa ggctctgcgt gaacggtacg aaaaagcaca caaagaactt tgtcgagtac
     6721 gtgacgaaca aaaaacaata gggaaaataa aagtaaatac aaatttttaa aacgttaata
     6781 taca
//
