LOCUS       CP103282                4123 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-569 plasmid unnamed4, complete
            sequence.
ACCESSION   CP103282
VERSION     CP103282.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323451
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 183x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 22:29:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,282
            CDSs (total)                      :: 4,183
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 99
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 158 of 194
            Pseudo Genes (incomplete)         :: 33 of 194
            Pseudo Genes (internal stop)      :: 20 of 194
            Pseudo Genes (multiple problems)  :: 14 of 194
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-569"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="2012-10-17"
     gene            complement(join(4031..4123,1..702))
                     /locus_tag="NXX16_21380"
     CDS             complement(join(4031..4123,1..702))
                     /locus_tag="NXX16_21380"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UVS59824.1"
                     /translation="MIGKGKSISHGVAALEYDLAKEINGQAVATEIARHELYGCTGAEM
                     VQEMKPYHIDFPNVKNNCLRFEVSPSIEESATFTDADWAELGNDFMQRMGLANHQYIII
                     RHSGTESKKEQAHLHILANRVSLSGELYRDNWIGKKATEAANAIAKERNFVQSQDIGKV
                     NKAEIKEAMDGVLKKMQGFDFTKFKEELGKRGFKVREARASTGKLNGYYVTARSGTEYK
                     ASEIGKGYTLAHIERTQSKLKCNSMNISHGNKLTPGSGSFQR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1191..1388)
                     /locus_tag="NXX16_21385"
     CDS             complement(1191..1388)
                     /locus_tag="NXX16_21385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS59825.1"
                     /translation="MVEYCVYWLENGEPMHEVFSSLAAAEMYSCAIRGKENIEWVEVSE
                     EETIDLDELEDMFPDDFCGV"
                     /name="hypothetical protein"
     gene            complement(1399..1674)
                     /locus_tag="NXX16_21390"
     CDS             complement(1399..1674)
                     /locus_tag="NXX16_21390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004519 - endonuclease activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family toxin"
                     /protein_id="UVS59826.1"
                     /translation="MMYTIRVSDGVDKVIAKWKKSNPNLFKKYKKIYKELLEHPKTGLG
                     HPEALRGGGDITWSRHITAHDRIIYDIYEEVVEVYILEVEGHYNDK"
                     /name="type II toxin-antitoxin system YoeB family toxin"
     gene            complement(1671..1922)
                     /locus_tag="NXX16_21395"
     CDS             complement(1671..1922)
                     /locus_tag="NXX16_21395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS59827.1"
                     /translation="MEALSVREYRNNLAASFTKADNGEQVLIRRKNEIYALVKVGREDL
                     MITPELQARIDKAREEIKSGKCVTLKSSEDIDAYFDSL"
                     /name="hypothetical protein"
     gene            2388..3335
                     /locus_tag="NXX16_21400"
     CDS             2388..3335
                     /locus_tag="NXX16_21400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UVS59821.1"
                     /translation="MSIHEQRIVLRILEACQAELKGVKLKDYAGTKRKFEHGLWDVDAQ
                     MHVSDVIFSGRDYNEIIAALDSLAGRFFTYEDDEEWWKCGFISNPKYKKRTGIITFRVS
                     NDLWDVFTKFAKGYREFELNKALALPTGYSLRFYMLMSGQVYPLDISLENLKDRLGIPA
                     DKYKDKNGKDRIDHFEERVLKPAKAALDESCPYTFNYVKVRENPNNKRSKVTGFRFYPV
                     YQPQFRDEELEGKELQAKVTARYQIDSHVYEYLRYSCGFTSEEINRNKETFITAQEKIT
                     DLIGELALLNGKSREKNNPKGWIINALKGKIKDK"
                     /name="replication initiation protein"
     gene            complement(3372..3602)
                     /locus_tag="NXX16_21405"
     CDS             complement(3372..3602)
                     /locus_tag="NXX16_21405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="charged multivesicular body protein 2b-B"
                     /protein_id="UVS59822.1"
                     /translation="MGTTEHEEPRFFFILNKGAKSGGEITHAVLNGSIVSKPAGWDAFH
                     GLALAREKLSSEEIQQQMKELGVEMEIVPLI"
                     /name="charged multivesicular body protein 2b-B"
     gene            complement(3622..4074)
                     /locus_tag="NXX16_21410"
     CDS             complement(3622..4074)
                     /locus_tag="NXX16_21410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS59823.1"
                     /translation="METNSHPEAAASNVKQGQYSPRKRKDTTPRVNPQLAVELVYQELK
                     RVEVYTKRIEDATARKVQIDGKSLESAENRLKNVLADFERQGYRMKNGGYVDKRISFYS
                     ILCAVISLLFACFMCYLWTDAAKDRDNYKQYYEYYQEQAREQKGNK"
                     /name="hypothetical protein"
     Typing          complement(2..702)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="82.479"
                     /coverage="89.0"
                     /evalue="1.6200000000000003e-132"
ORIGIN
        1 atgggcgaga gtgtagcctt tccctatctc gcttgcctta tactccgtac cgcttcgggc
       61 tgtgacatag tagccgttaa gtttcccggt gcttgccctg gcttcccgga ctttaaaacc
      121 tctcttacca agttcttcct tgaatttggt aaagtcaaag ccctgcatct tcttcaatac
      181 gccgtccatg gcttccttga tttccgcttt gttgactttg ccaatgtcct gcgactgtac
      241 aaagttccgt tccttggcta tggcattggc ggcttccgtt gcctttttcc ctatccagtt
      301 gtcccggtac agttccccgg aaagggaaac ccggtttgcc agtatgtgca ggtgggcttg
      361 ctctttcttg ctctccgtac cgctgtgccg gatgatgatg tactggtggt tcgccaaccc
      421 catgcgctgc atgaagtcgt taccaagttc cgcccagtcc gcatccgtaa aggtggcgct
      481 ttcctctatc gaggggctga cctcgaaacg taggcagttg ttttttacgt tcggaaaatc
      541 aatgtgatac ggtttcattt cctgcaccat ttccgcacca gtacacccat aaagttcatg
      601 ccgggctatc tcggtggcta ctgcctgccc gtttatctct ttggcgaggt cgtactccaa
      661 tgccgccaca ccatgtgata tgcttttccc tttccctatc atcgtgagag tatttttttg
      721 agctctgtaa ttagtgcccg gttctcctcg aacacttccc ggtactggcg accaccgaag
      781 tagttgttca gccgttggag cgttcctttc aactgggcta tgtcccggag aagctgccgt
      841 tcctcctcgg tgtacctctc ccttgggcgc cccccaaggg acaaactccg gcagtattcc
      901 gatacgctta gactgcaccg ggctgcctgc tccgcaagtg cgtccttttc aagttcggta
      961 caccggaatg taatcttttc gttccgtctt agttccatgc cacaaaggta atcttaaaat
     1021 ctttttgagc aaagcgagaa aagcaagaat tgtccgacaa ggacacttct tgctatatat
     1081 acacatgccg catgggaata catccacgcc aaccgcccag caaaaagccc cggcgtgtta
     1141 gaaaatcccc cacagcctaa caaattctac cccatttcta cccaagggga ttacacgccg
     1201 cagaagtcat ccgggaacat gtcttccagt tcgtccaaat caatggtttc ttcttcggac
     1261 acctccaccc attcaatgtt ttctttccct cttatcgcac atgagtacat ctcggcggcg
     1321 gcaagagaag agaacacctc gtgcataggc tcgccgttct ctaaccagta aacacaatat
     1381 tcgaccataa cagttttatc atttatcgtt gtaatgcccc tctacctcca agatgtagac
     1441 ctctaccact tcctcgtaaa tgtcatagat tatccggtca tgggcggtaa tgtgtcgaga
     1501 ccatgtaata tccccaccgc cccttaacgc ttcgggatgc ccaagcccag tcttagggtg
     1561 ctctaacaat tctttgtaga ttttcttata cttcttgaac aaattggggt ttgatttctt
     1621 ccatttggca atcaccttat ccaccccgtc ggaaacccta attgtgtaca tcatagacta
     1681 tcgaaataag cgtcaatatc ctcactgctt ttgagggtaa cacactttcc ggatttgatt
     1741 tcttcccttg ccttgtcaat ccttgcttgc agctccgggg ttatcatcaa atcttcacga
     1801 ccaactttta ccaaagcgta aatctcgttt ttcctgcgaa ttagtacctg ctcgccattg
     1861 tcagctttgg tgaaagacgc tgcgaggttg ttacggtatt ctcttactga taatgcttcc
     1921 atatctgttt gcttttaaat tcagcacaaa gatagctata tttcaataaa atacaaacat
     1981 tttgtacaca aacgtgtaca cgccataaaa acccgtttcc aatcctaccg cccgttggtt
     2041 ggttttgctt tgctcttttt ccctatcgtt tttctttttc cgacagtaaa tcaaccactt
     2101 agctctataa attccccttg tctgttataa gcatcgtctg taatatgctc tatcacttct
     2161 tcgcaaacgc ccataaataa agggattaga acatgtgttt ccccacactt tgggagttta
     2221 gtccccacac tttgggagtt taaaagtaaa ttagtcccca cactttggga gttttacccc
     2281 tctttttttg ttttaatgtg gggattgtgt atatttgcag caaatcaatc gtttagaata
     2341 aagatgttgt tgtttcatgg gtatatacat ggtcaaaaca gcaagacatg tccatacacg
     2401 aacaaagaat agttcttcgc atactggaag cgtgccaagc tgaactaaag ggagtaaaac
     2461 tgaaagatta tgcaggcacg aaacgcaagt ttgagcatgg actttgggat gttgatgcac
     2521 aaatgcatgt atctgacgtt attttttccg gacgtgatta caatgaaatc atagccgcac
     2581 tcgattcttt ggcaggacgt ttttttactt acgaagatga cgaggaatgg tggaagtgcg
     2641 gatttatatc taacccaaaa tataaaaaac gcactggcat tataaccttt cgtgtgtcta
     2701 acgacctttg ggatgtgttt accaagttcg ccaaggggta cagagaattt gagttaaaca
     2761 aggctcttgc gctacctact gggtattccc ttaggtttta catgttaatg agtgggcagg
     2821 tgtacccttt ggatatatcc cttgaaaact tgaaagaccg tcttggcata cctgcggaca
     2881 aatacaagga caagaacgga aaagacagaa tagatcattt tgaggaaaga gttttgaaac
     2941 cagcaaaggc tgcgcttgat gagagctgcc catacacttt caactacgtg aaagtgcgtg
     3001 aaaacccaaa caacaaacgt agcaaggtga ccgggtttag gttctacccg gtctatcaac
     3061 cacagttcag agatgaagaa ctggaaggga aagaattgca agcaaaggtt acagcacgat
     3121 atcagataga cagccatgtg tacgagtacc tccgttattc ctgcggcttc acatcggaag
     3181 agataaaccg gaacaaagag acattcataa cggcacaaga aaagataacc gaccttatcg
     3241 gagaactggc tctcctaaac ggaaaatcac gagaaaagaa caatccgaaa gggtggatta
     3301 taaacgccct taaagggaaa atcaaggata agtaacaaga ttacccggct gaaacactcc
     3361 gggtaatctt gtcaaatcag aggaacgatt tccatttcta caccaagttc tttcatctgc
     3421 tgttgtattt cctcgctgct tagtttctct ctcgccagtg caagcccatg aaaggcgtcc
     3481 cagcctgccg gcttcgaaac gatactaccg tttaggacgg catgggtgat ttcgccgccg
     3541 gacttcgcac ccttgttcag tatgaagaag aaccggggtt cttcgtgttc cgttgttccc
     3601 ataattcgta aagttagatg tttatttatt ccccttttgt tcccttgcct gctcttggta
     3661 gtattcataa tactgcttgt agttgtcccg gtctttggct gcatccgtcc aaagatagca
     3721 catgaaacaa gcgaacaata gggagataac agcacaaagg attgagtaga acgaaattcg
     3781 tttgtccaca taaccgccgt ttttcattct gtacccctgc cgttcaaagt ccgccaacac
     3841 gttttttagg cgattttcgg cactttcaag gcttttcccg tctatttgta cctttcgggc
     3901 tgttgcgtcc tctatgcgct tcgtatagac ttcaacacgc tttagttctt gatacaccag
     3961 ttctaccgca agctgcgggt ttacccttgg cgttgtatct tttcttttcc gggggctgta
     4021 ctgtccctgc ttaacgttgg aagctgccgc ttccgggtgt gagtttgttt ccatgagata
     4081 tgttcattga gttgcacttc agtttgcttt gtgtccgctc gat
//
