LOCUS       CP103281                2727 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-569 plasmid unnamed3, complete
            sequence.
ACCESSION   CP103281
VERSION     CP103281.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323451
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 183x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 22:29:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,282
            CDSs (total)                      :: 4,183
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 99
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 158 of 194
            Pseudo Genes (incomplete)         :: 33 of 194
            Pseudo Genes (internal stop)      :: 20 of 194
            Pseudo Genes (multiple problems)  :: 14 of 194
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-569"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="2012-10-17"
     gene            7..597
                     /locus_tag="NXX16_21370"
     CDS             7..597
                     /locus_tag="NXX16_21370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA protein"
                     /protein_id="UVS59819.1"
                     /translation="MENKKVLKLTDFQKNEENPFMKQAIEGIENHVVKKYKSSTGSDKK
                     AVVAVADTDTGEVFKTSFIRQIEVDEEQFAKLYLANFAAFFNLSQSAIRVFGYILTCMK
                     PKNDMIIFDRRKCLEYTQYKSDKAIYKGLAELVQSEIIARGPNEYNWFINPLIVFNGDR
                     VSFTKTYVRKKTLAAQKKEEAEKRQLSLGFDEL"
                     /name="RepA protein"
     gene            1215..2261
                     /locus_tag="NXX16_21375"
     CDS             1215..2261
                     /locus_tag="NXX16_21375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="UVS59820.1"
                     /translation="MGATSIHVQAVKPGSEIHSWVGDSISHRLESAKQRYFDTVGQKMQ
                     TKAAPIREGVIVIKQETTMQELQQFAAVCKERFGIEAFQIHIHKDEGYMNAKQWTPNLH
                     AHVVFDWTQPNGKSVRLSRDDMAELQTIASEALGMERGVSSDRKHLSAMQYKTECAKEQ
                     LQELSNDISSALDKHKDVQNQLLQLQKELRSIETKKNVQKLISKASEKFYGLIGKTVND
                     REKDALKAKIKALEGENEQLSDRLGKAILEKERNGTKVFKAENDKEYYRQQMDNARTTS
                     NRLRTENQELKTETKELKKELGKMKDLFNSEQLEALRHHFPNISKAMEEGKDLLKQITR
                     SRGFGMGM"
                     /name="mobilization protein"
     Typing          8..587
                     /name="rep_cluster_1868"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.479"
                     /coverage="95.0"
                     /evalue="2.71e-121"
     Typing          1216..2258
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.736"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtttatgg aaaataagaa agtactcaaa ttaaccgatt ttcaaaaaaa cgaagaaaac
       61 ccctttatga aacaagcaat tgaagggatt gaaaatcacg ttgttaagaa atacaaaagt
      121 agtacgggaa gtgataaaaa agctgtagtt gctgttgctg atacagatac aggagaagta
      181 ttcaaaacct cgtttatccg acaaatagaa gtagatgaag agcaatttgc aaaactatac
      241 cttgcgaatt ttgccgcttt tttcaatcta tcacaatcag ctattcgggt tttcgggtat
      301 attctaactt gcatgaagcc taagaatgat atgataattt tcgataggcg aaaatgctta
      361 gaatacaccc aatacaaatc agacaaagcc atatacaaag gattggcaga gctcgtacaa
      421 agtgaaatca tagcaagagg tccaaacgaa tataattggt tcattaatcc gttgattgtc
      481 tttaatgggg atagggtttc atttacaaaa acatacgttc ggaaaaagac tttagctgcc
      541 caaaagaaag aagaagcaga gaaacgacag ttatcacttg gttttgatga actgtaacac
      601 tccattgagt gaagctgccg tttggtcgct ccccctttgg gggcgggggg ggatagataa
      661 agttcctcta tgtaaagtta taatggggga tgaaaggcaa ggttcgctaa ccttaccccg
      721 attgtttatg cttcccagca ttcaatctat ccccgtttat gggtttctct ttcaccctac
      781 aaagataacc tcatggggga aaaatgtcca agaatatggg gtaaaaacta tcaagtcggt
      841 agaaaataag tatctttgaa gtacattttt agtagaggta ctggtatgcc tagacgaagc
      901 agataggcaa aaataaagcc gctggggatg tcggatgtcc gtcaattaac tccagcggct
      961 tgtattttgg taactctccc gtatcggtca gctaccgttt ggtcagcaac aaaggtagta
     1021 ctttattgtc gcaaatccaa atgttttccc ctttatttgt gcatttacaa aacattcact
     1081 aacttcgcac ctatgcagta tggtgatagc acactagcac cctaaagggt acgtgtgtaa
     1141 ggggacaagc ccccttaacc ccctgtcagc cggctgtcac cggctgttat cgattgtcac
     1201 aaaaatgtta acttatggga gcaacaagta ttcatgtaca agcagtgaag ccggggagcg
     1261 agattcacag ctgggttgga gatagcatct cccatcggct ggagagtgcg aaacagagat
     1321 atttcgatac ggttgggcag aagatgcaga ctaaagcagc acccatacgg gagggggtaa
     1381 tagtaatcaa gcaagaaacc actatgcagg aactccagca attcgctgcg gtctgcaagg
     1441 aacgtttcgg tattgaagcg tttcaaatcc atatacacaa ggacgaagga tacatgaacg
     1501 caaagcaatg gacacctaac cttcacgccc atgtagtttt cgattggacg cagccgaacg
     1561 ggaagagtgt gcgtttatcg cgtgatgaca tggcagaact ccaaaccata gcatctgaag
     1621 cactgggcat ggaacgtggt gtttcttctg atcgcaaaca cttatcggct atgcagtaca
     1681 agaccgaatg tgcgaaagaa cagcttcagg aactatccaa cgatatatcc agtgcgttag
     1741 acaagcacaa ggacgtgcaa aaccagcttc ttcagcttca gaaagaacta cgttccattg
     1801 aaacaaagaa aaacgtccaa aaactcattt ctaaggcttc agagaagttt tacggcttga
     1861 tagggaaaac ggttaacgat agggaaaaag atgccttaaa agccaaaata aaggctttag
     1921 agggggaaaa tgaacagcta tccgatagac tgggaaaggc tatacttgaa aaagagcgaa
     1981 acggcacaaa ggtattcaaa gccgagaacg acaaggagta ttatagacag caaatggaca
     2041 atgcaaggac aaccagtaac cgactaagaa cggagaacca agaactaaag accgaaacca
     2101 aggaattgaa aaaagaactt ggtaagatga aagacttgtt caattccgaa caactggagg
     2161 ctttaaggca tcatttccca aacatatcaa aagctatgga agaggggaaa gacctactca
     2221 agcaaatcac cagaagcaga ggtttcggca tgggtatgta accccccccc gcccccaaag
     2281 ggggagcgac caaacggcag cttcactcaa tggagtgtta cgccgttcgg gttcggaaga
     2341 aattttgcag ggttcgaaaa acggttgtgt gcttattcga aaaaaagttt atggtgtggg
     2401 cgctcgtcgg gacgggtggt cccgcccctt gccgctgggc gctccccgac cgatgatttt
     2461 ttaagtagtt gattatatgc cgtttttagg gtataataag aacttaagaa atagaataag
     2521 ttaagtagtt gatactcaat atagggtatt ttccatattg gaaaatctga ttttccatat
     2581 tagaaagtca ctctttccta ttagatgtta aaaaggaaaa tatattttgc ttttttattg
     2641 tattaatgga aatttaaata ttcctttgtg gaaaatcaaa ttatactttt tcaccgatat
     2701 atagaaagtc aaatttgcct aaataga
//
