LOCUS       CP103263                4173 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-526 plasmid unnamed5, complete
            sequence.
ACCESSION   CP103263
VERSION     CP103263.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323426
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 29x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 22:04:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,812
            CDSs (total)                      :: 4,720
            Genes (coding)                    :: 4,479
            CDSs (with protein)               :: 4,479
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 241
            CDSs (without protein)            :: 241
            Pseudo Genes (ambiguous residues) :: 0 of 241
            Pseudo Genes (frameshifted)       :: 186 of 241
            Pseudo Genes (incomplete)         :: 64 of 241
            Pseudo Genes (internal stop)      :: 11 of 241
            Pseudo Genes (multiple problems)  :: 17 of 241
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-526"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="2009-08-31"
     gene            join(3891..4173,1..170)
                     /locus_tag="NXV60_24030"
     CDS             join(3891..4173,1..170)
                     /locus_tag="NXV60_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS41800.1"
                     /translation="METNSHPEAAASNVKQGQYSPRKRKDTTPRVNPQLAVELVYQELK
                     RVEVYTKRIEDATARKVQIDGKSLESAENRLKNVLADFERQGYRMKNGGYVDKRISFYS
                     ILCAVISLLFACFMCYLWTDAAKDRDNYKQYYEYYQEQAREQKGNK"
                     /name="hypothetical protein"
     gene            190..420
                     /locus_tag="NXV60_24035"
     CDS             190..420
                     /locus_tag="NXV60_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="charged multivesicular body protein 2b-B"
                     /protein_id="UVS41801.1"
                     /translation="MGTTEHEEPRFFFILNKGAKSGGEITHAVLNGSIVSKPAGWDAFH
                     GLALAREKLSSEEIQQQMKELGVEMEIVPLI"
                     /name="charged multivesicular body protein 2b-B"
     gene            complement(457..1482)
                     /locus_tag="NXV60_24040"
     CDS             complement(457..1482)
                     /locus_tag="NXV60_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UVS41802.1"
                     /translation="MKKKLPITKNKDVVVSWVYTWSKQQDMSIHEQRIVLRILEACQAE
                     LKGVKLKDYAGTKRKFEHGLWDVDAQMHVSDVIFSGRDYNEIIAALDSLAGRFFTYEDD
                     EEWWKCGFISNPKYKKRTGIITFRVSNDLWDVFTKFAKGYREFELNKALALPTGYSLRF
                     YMLMSGQVYPLDISLENLKDRLGIPADKYKDKNGKDRIDHFEERVLKPAKAALDESCPY
                     TFNYVKVRENPNNKRSKVTGFRFYPVYQPQFRDEELEGKELQAKVTARYQIDSHVYEYL
                     RYSCGFTSEEINRNKETFITAQEKITDLIGELALLNGKSREKNNPKGWIINALKGKIKD
                     K"
                     /name="replication initiation protein"
     gene            1895..2146
                     /locus_tag="NXV60_24045"
     CDS             1895..2146
                     /locus_tag="NXV60_24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS41803.1"
                     /translation="MEALSVREYRNNLAASFTKADNGEQVLIRRKNEIYALVKVGREDL
                     MITPELQARIDKAREEIKSGKCVTLKSSEDIDAYFDSL"
                     /name="hypothetical protein"
     gene            2143..2418
                     /locus_tag="NXV60_24050"
     CDS             2143..2418
                     /locus_tag="NXV60_24050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004519 - endonuclease activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family toxin"
                     /protein_id="UVS41798.1"
                     /translation="MMYTIRVSDGVDKVIAKWKKSNPNLFKKYKKIYKELLEHPKTGLG
                     HPEALRGGGDITWSRHITAHDRIIYDIYEEVVEVYILEVEGHYNDK"
                     /name="type II toxin-antitoxin system YoeB family toxin"
     gene            2454..2651
                     /locus_tag="NXV60_24055"
     CDS             2454..2651
                     /locus_tag="NXV60_24055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS41804.1"
                     /translation="MVEYCVYWLENGEPMHEVFSSLAAAEMYSCAIRGKENIEWVEVSE
                     EETIDLDELEDMFPDDFCGV"
                     /name="hypothetical protein"
     gene            3140..3934
                     /locus_tag="NXV60_24060"
     CDS             3140..3934
                     /locus_tag="NXV60_24060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UVS41799.1"
                     /translation="MIGKGKSISHGVAALEYDLAKEINGQAVATEIARHELYGCTGAEM
                     VQEMKPYHIDFPNVKNNCLRFEVSPSIEESATFTDADWAELGNDFMQRMGLANHQYIII
                     RHSGTESKKEQAHLHILANRVSLSGELYRDNWIGKKATEAANAIAKERNFVQSQDIGKV
                     NKAEIKEAMDGVLKKMQGFDFTKFKEELGKRGFKVREARASTGKLNGYYVTARSGTEYK
                     ASEIGKGYTLAHIERTQSKLKCNSMNISHGNKLTPGSGSFQR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
ORIGIN
        1 atgtggacaa acgaatttcg ttctactcaa tcctttgtgc tgttatctcc ctattgttcg
       61 cttgtttcat gtgctatctt tggacggatg cagccaaaga ccgggacaac tacaagcagt
      121 attatgaata ctaccaagag caggcaaggg aacaaaaggg gaataaataa acatctaact
      181 ttacgaatta tgggaacaac ggaacacgaa gaaccccggt tcttcttcat actgaacaag
      241 ggtgcgaagt ccggcggcga aatcacccat gccgtcctaa acggtagtat cgtttcgaag
      301 ccggcaggct gggacgcctt tcatgggctt gcactggcga gagagaaact aagcagcgag
      361 gaaatacaac agcagatgaa agaacttggt gtagaaatgg aaatcgttcc tctgatttga
      421 caagattacc cggagtgttt cagccgggta atcttgttac ttatccttga ttttcccttt
      481 aagggcgttt ataatccacc ctttcggatt gttcttttct cgtgattttc cgtttaggag
      541 agccagttct ccgataaggt cggttatctt ttcttgtgcc gttatgaatg tctctttgtt
      601 ccggtttatc tcttccgatg tgaagccgca ggaataacgg aggtactcgt acacatggct
      661 gtctatctga tatcgtgctg taacctttgc ttgcaattct ttcccttcca gttcttcatc
      721 tctgaactgt ggttgataga ccgggtagaa cctaaacccg gtcaccttgc tacgtttgtt
      781 gtttgggttt tcacgcactt tcacgtagtt gaaagtgtat gggcagctct catcaagcgc
      841 agcctttgct ggtttcaaaa ctctttcctc aaaatgatct attctgtctt ttccgttctt
      901 gtccttgtat ttgtccgcag gtatgccaag acggtctttc aagttttcaa gggatatatc
      961 caaagggtac acctgcccac tcattaacat gtaaaaccta agggaatacc cagtaggtag
     1021 cgcaagagcc ttgtttaact caaattctct gtaccccttg gcgaacttgg taaacacatc
     1081 ccaaaggtcg ttagacacac gaaaggttat aatgccagtg cgttttttat attttgggtt
     1141 agatataaat ccgcacttcc accattcctc gtcatcttcg taagtaaaaa aacgtcctgc
     1201 caaagaatcg agtgcggcta tgatttcatt gtaatcacgt ccggaaaaaa taacgtcaga
     1261 tacatgcatt tgtgcatcaa catcccaaag tccatgctca aacttgcgtt tcgtgcctgc
     1321 ataatctttc agttttactc cctttagttc agcttggcac gcttccagta tgcgaagaac
     1381 tattctttgt tcgtgtatgg acatgtcttg ctgttttgac catgtatata cccatgaaac
     1441 aacaacatct ttattcttgg taataggtag tttttttttc ataaacgatt gatttgctgc
     1501 aaatatacac aatccccaca ttaaaacaaa aaaagagggg taaaactccc aaagtgtggg
     1561 gactaattta cttttaaact cccaaagtgt ggggactaaa ctcccaaagt gtggggaaac
     1621 acatgttcta atccctttat ttatgggcgt ttgcgaagaa gtgatagagc atattacaga
     1681 cgatgcttat aacagacaag gggaatttat agagctaagt ggttgattta ctgtcggaaa
     1741 aagaaaaacg atagggaaaa agagcaaagc aaaaccaacc aacgggcggt aggattggaa
     1801 acgggttttt atggcgtgta cacgtttgtg tacaaaatgt ttgtatttta ttgaaatata
     1861 gctatctttg tgctgaattt aaaagcaaac agatatggaa gcattatcag taagagaata
     1921 ccgtaacaac ctcgcagcgt ctttcaccaa agctgacaat ggcgagcagg tactaattcg
     1981 caggaaaaac gagatttacg ctttggtaaa agttggtcgt gaagatttga tgataacccc
     2041 ggagctgcaa gcaaggattg acaaggcaag ggaagaaatc aaatccggaa agtgtgttac
     2101 cctcaaaagc agtgaggata ttgacgctta tttcgatagt ctatgatgta cacaattagg
     2161 gtttccgacg gggtggataa ggtgattgcc aaatggaaga aatcaaaccc caatttgttc
     2221 aagaagtata agaaaatcta caaagaattg ttagagcacc ctaagactgg gcttgggcat
     2281 cccgaagcgt taaggggcgg tggggatatt acatggtctc gacacattac cgcccatgac
     2341 cggataatct atgacattta cgaggaagtg gtagaggtct acatcttgga ggtagagggg
     2401 cattacaacg ataaatgata aaactgttat ggtcgaatat tgtgtttact gttatggtcg
     2461 aatattgtgt ttactggtta gagaacggcg agcctatgca cgaggtgttc tcttctcttg
     2521 ccgccgccga gatgtactca tgtgcgataa gagggaaaga aaacattgaa tgggtggagg
     2581 tgtccgaaga agaaaccatt gatttggacg aactggaaga catgttcccg gatgacttct
     2641 gcggcgtgta atccccttgg gtagaaatgg ggtagaattt gttaggctgt gggggatttt
     2701 ctaacacgcc ggggcttttt gctgggcggt tggcgtggat gtattcccat gcggcatgtg
     2761 tatatatagc aagaagtgtc cttgtcggac aattcttgct tttctcgctt tgctcaaaaa
     2821 gattttaaga ttacctttgt ggcatggaac taagacggaa cgaaaagatt acattccggt
     2881 gtaccgaact tgaaaaggac gcacttgcgg agcaggcagc ccggtgcagt ctaagcgtat
     2941 cggaatactg ccggagtttg tcccttgggg ggcgcccaag ggagaggtac accgaggagg
     3001 aacggcagct tctccgggac atagcccagt tgaaaggaac gctccaacgg ctgaacaact
     3061 acttcggtgg tcgccagtac cgggaagtgt tcgaggagaa ccgggcacta attacagagc
     3121 tcaaaaaaat actctcacga tgatagggaa agggaaaagc atatcacatg gtgtggcggc
     3181 attggagtac gacctcgcca aagagataaa cgggcaggca gtagccaccg agatagcccg
     3241 gcatgaactt tatgggtgta ctggtgcgga aatggtgcag gaaatgaaac cgtatcacat
     3301 tgattttccg aacgtaaaaa acaactgcct acgtttcgag gtcagcccct cgatagagga
     3361 aagcgccacc tttacggatg cggactgggc ggaacttggt aacgacttca tgcagcgcat
     3421 ggggttggcg aaccaccagt acatcatcat ccggcacagc ggtacggaga gcaagaaaga
     3481 gcaagcccac ctgcacatac tggcaaaccg ggtttccctt tccggggaac tgtaccggga
     3541 caactggata gggaaaaagg caacggaagc cgccaatgcc atagccaagg aacggaactt
     3601 tgtacagtcg caggacattg gcaaagtcaa caaagcggaa atcaaggaag ccatggacgg
     3661 cgtattgaag aagatgcagg gctttgactt taccaaattc aaggaagaac ttggtaagag
     3721 aggttttaaa gtccgggaag ccagggcaag caccgggaaa cttaacggct actatgtcac
     3781 agcccgaagc ggtacggagt ataaggcaag cgagataggg aaaggctaca ctctcgccca
     3841 tatcgagcgg acacaaagca aactgaagtg caactcaatg aacatatctc atggaaacaa
     3901 actcacaccc ggaagcggca gcttccaacg ttaagcaggg acagtacagc ccccggaaaa
     3961 gaaaagatac aacgccaagg gtaaacccgc agcttgcggt agaactggtg tatcaagaac
     4021 taaagcgtgt tgaagtctat acgaagcgca tagaggacgc aacagcccga aaggtacaaa
     4081 tagacgggaa aagccttgaa agtgccgaaa atcgcctaaa aaacgtgttg gcggactttg
     4141 aacggcaggg gtacagaatg aaaaacggcg gtt
//
