LOCUS       CP103261                8328 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-526 plasmid unnamed3, complete
            sequence.
ACCESSION   CP103261
VERSION     CP103261.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323426
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8328)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 29x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 22:04:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,812
            CDSs (total)                      :: 4,720
            Genes (coding)                    :: 4,479
            CDSs (with protein)               :: 4,479
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 241
            CDSs (without protein)            :: 241
            Pseudo Genes (ambiguous residues) :: 0 of 241
            Pseudo Genes (frameshifted)       :: 186 of 241
            Pseudo Genes (incomplete)         :: 64 of 241
            Pseudo Genes (internal stop)      :: 11 of 241
            Pseudo Genes (multiple problems)  :: 17 of 241
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-526"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="2009-08-31"
     gene            join(8281..8328,1..222)
                     /locus_tag="NXV60_23740"
     CDS             join(8281..8328,1..222)
                     /locus_tag="NXV60_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018967844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="UVS41746.1"
                     /translation="MYEIEFSKEAVKHVLLMRKSSPQLFKKLEKLLDELREHPYTGTGH
                     PEQLRYLEGVWSRQLDKKNRIRYMVNETTVIVFIISAWGHYDDK"
                     /name="Txe/YoeB family addiction module toxin"
     gene            676..1278
                     /locus_tag="NXV60_23745"
     CDS             676..1278
                     /locus_tag="NXV60_23745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clindamycin resistance transfer factor btgA"
                     /protein_id="UVS41747.1"
                     /translation="MEKDSKTTVAVERTTFTKLDRLAKANNVSKMEFLTHAINYFEKYG
                     INPVEHESPAQEMQKLIKRMDQVFAFLKKQETDLVRPACEALAGASTQITISLSSLLSE
                     EKFRRFLKDNKELLAEIINSSDNNGKAIGRTEKAVRDADSTIRKNQDIIFKRIEASETA
                     QIKAYKHIVSFLDAKGKTGLLDDINKVYEREKERRGL"
                     /name="clindamycin resistance transfer factor btgA"
     gene            1275..2174
                     /locus_tag="NXV60_23750"
     CDS             1275..2174
                     /locus_tag="NXV60_23750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5712 family protein"
                     /protein_id="UVS41748.1"
                     /translation="MNIKIQGGGSGTYANTGSCTGVTTYLQHEDLERMKNGREVQPFFN
                     NSRDYISAQEVTFKIDNNKAKLSRNDAKFYVITVSPSSRELEKMGKTEKEQAEAMRRYV
                     RDDVMQHYAEGFGKGLNKEDIEYYGKIHFERKGADRYDMHAHIIVSRKDRSNTRKLSPK
                     TNHTGKKNCGNVKGGFDRTDFFRKCESSFDKRTGYDRAPEQTFDYLNTMKNGSPKEIFQ
                     KKEWAERVNHERLEKMKAEWSRDLQEPHQEQGREESQQQGNSISQVPDINQAPQRKKQQ
                     EEELDQPRKRSRGVGMGM"
                     /name="DUF5712 family protein"
     gene            complement(2259..3464)
                     /locus_tag="NXV60_23755"
     CDS             complement(2259..3464)
                     /locus_tag="NXV60_23755"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS41749.1"
                     /translation="MNLKSNVCLIIQEDFDIQNGRKTSSNTTIFKFNHHKNLLKEIKGK
                     EITSYSYDNSGRLIVEKHSKDIITLSEETDFINVSDYKKYYYKDENVFPFKIEEYYGNE
                     NSLNAKTIIERDKNNYKTKTIKVEYKKDPFSGKAINDTTIMLFKNNGNNKYTEIEIRQG
                     KRILLEKYEINENGQITSKHIKDLNVITDTIPTYILEDIKTWKQKKSYNYVKYDNNLLT
                     TYERNDNGDLVHILDVDSTENNQHETYAQYTYDERGNWTKREIFNSLHELIAIATRKIE
                     YYPQKENGEIDLSFEHETTPLEKEFEREQRIKQKEELYLNDNFIIQQFHEKMKEYPDYR
                     VIGEPKITYHDGYTYNINFNATHYFGGYPNGYPSKENITVQIILDLETETYTFTTIKGD
                     LY"
                     /name="hypothetical protein"
     gene            complement(3476..4375)
                     /locus_tag="NXV60_23760"
     CDS             complement(3476..4375)
                     /locus_tag="NXV60_23760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="UVS41750.1"
                     /translation="MLKHSVQIELFLILWVTKLIYSESPMYTVLDKDTIKNEILPHLSK
                     AKRGYVTQSCLIEVVNAILYKLKTGCQWAFLPVEALFSGKVLSYGAVYHHYNKWSKSGE
                     WKSMWVRLLDKHRHELDMSSVDLDGSHTAALRGGEEVGYQGRKKRKTTNALYLTDRQGL
                     PIAMSSPKSGEHHDVHDIEKVIGDMFNDLEKSHIRVDGLFLNADAGFDCDVLRGFLCEK
                     EVIANICINKRRENADDIFVDDELYAERYSIERTYAWMDSFRTLLNRFDTTVSSWMSWN
                     YLAFAVLLLKKISRKQKV"
                     /name="IS5 family transposase"
     gene            4623..5423
                     /gene="erm(F)"
                     /locus_tag="NXV60_23765"
     ARG             4624..5420
                     /name="erm(F)"
                     /gene="erm(F)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(F)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="99.25"
                     /coverage="100.0"
     gene            complement(5796..6305)
                     /locus_tag="NXV60_23770"
     CDS             complement(5796..6305)
                     /locus_tag="NXV60_23770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007844266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS41742.1"
                     /translation="MQEKTNIQTSTLRVPKNILEEIKIYCLKAGKPVGEWAETAWKFIE
                     KNDFDIYDKETTPFLPVSPDIEKERNQVEALCMLMSEFITAQKQIQFPAPELIAKAAEE
                     KVRAEMKAEEQAKDLQVLQEENNRLRNEIKVLQEYKEKAYRELCRVRDEQKTFGKIKVN
                     TELWKG"
                     /name="hypothetical protein"
     gene            complement(6373..6558)
                     /locus_tag="NXV60_23775"
     CDS             complement(6373..6558)
                     /locus_tag="NXV60_23775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008782590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS41743.1"
                     /translation="MKEGDLILVSAEATGLGKPMEAVIDKIETFMGQTLVTVTYTQPDA
                     LSGFGGCFVDVHITKQ"
                     /name="hypothetical protein"
     gene            complement(6589..7569)
                     /locus_tag="NXV60_23780"
     CDS             complement(6589..7569)
                     /locus_tag="NXV60_23780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UVS41744.1"
                     /translation="MAIVNKDLIQSYIMTTAKYDFNVYEKRIIYRLVEMAQGELQGIKF
                     ADNCQKIEHDLFSCVNITMPIACLLNGEDDKNYARVKEALQSLQRKIFEYEDDEIWQSI
                     SIIALPYIRKRSSVLSFQVHPRVWDCILDFSKGFRKYELKAAMSFKSQFSMRFYELLSN
                     QKTPLIYSIEQLKEMFCVADKYERINDFMRKVVEAAKKELDEVSPYTFEYTPLKTGRKI
                     TAIKFYPVYQPEHRDSDLEKHDLQKQTALSWSLTPEIRSYLKNSIEFSDKEIKNNLDLF
                     ISAQTLLPDFLNELAILKGKSREKKNPKGYIINAIKGKIKDRKKI"
                     /name="replication initiation protein"
     gene            8022..8288
                     /locus_tag="NXV60_23785"
     CDS             8022..8288
                     /locus_tag="NXV60_23785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="UVS41745.1"
                     /translation="MQIVTTREFRANQKKYFEMAETETILISRRNAAPIMVCAVREGDF
                     PSREELAAIQRGIDDVRNGNTFRMAKNESLDDFLNRIEGEGNV"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     ARG             4624..5423
                     /name="ErmF"
                     /gene="ErmF"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="98.87"
                     /coverage="100.0"
     Typing          complement(6590..7569)
                     /name="rep_cluster_1079"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.19"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1276..2171
                     /name="MOBB"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.993"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagaaaat cctctccgca gcttttcaag aagctggaga agctgcttga tgaactcaga
       61 gaacacccat atacgggtac aggacatccg gaacagttaa gatacctgga gggtgtctgg
      121 tcaaggcagc tggataagaa aaaccgtatc aggtatatgg tcaacgagac tacagtaatt
      181 gtgttcatca tttcagcctg ggggcattat gacgacaaat gaaggattgc agtatgtgcc
      241 gttttggaac ttactgcaat ccttttgaat ttgtgacaga atcaaaataa cttatatcat
      301 agatccaagc aaacggctgc actcgtcggg agtgttggta cacccccttg ccgtgcggcg
      361 ctccccgacc gatgagtttt ttgtggtgtt ccggacatgt tctgttgtgc ggttttcgtg
      421 tgtgttcacg tgtgtgggat aggttctatc tctccccgtc ccctttgctg atttcgcgct
      481 ggggaaggtg ttcgatagaa cagcaagagg aaaccgggca agccctgttt tttcctcttg
      541 ccctacgggg tttttagctg taaatcagct atttagaaaa ttatactaat ataatttggt
      601 tataatgaat aaatatacta tcttgtaaac caataagtta gtatattaat tcattataat
      661 aagccttata atatcatgga aaaagacagc aaaacaacgg tagcagtaga acgtactact
      721 tttactaaac ttgaccgtct ggccaaggca aacaatgtgt caaaaatgga gttccttaca
      781 catgctataa actattttga gaagtacggt ataaacccgg tcgagcatga aagcccagca
      841 caggaaatgc agaaacttat caagcgcatg gatcaggtat tcgcattcct gaagaaacag
      901 gaaacggatc ttgtgcgtcc ggcttgtgaa gctctggccg gagccagcac acagataacc
      961 ataagccttt catccttact ttcggaagaa aagttcaggc ggttcctgaa ggacaacaag
     1021 gagctgcttg ccgagattat caacagttcc gacaacaacg gcaaggccat aggccggaca
     1081 gaaaaagcgg tgcgggatgc agattcaact atcaggaaga accaggatat tatcttcaag
     1141 cggatagaag ccagcgaaac ggcacagatt aaagcctata agcatatcgt ttctttcctc
     1201 gatgcaaaag gaaagaccgg gctgctcgat gacataaaca aggtctatga gagggaaaag
     1261 gaaaggaggg ggctatgaac ataaaaattc agggtggcgg ttccggaact tatgccaata
     1321 cagggagctg cacaggagta acgacctatc tccagcatga ggatctggag cgcatgaaga
     1381 acggccggga agtgcagccg tttttcaata actctcgtga ctacataagc gcacaggaag
     1441 taaccttcaa gatagacaac aacaaggcaa aattaagccg gaacgatgca aagttctatg
     1501 tgattacggt aagcccttcc agccgggagc tggagaaaat gggcaagaca gagaaggaac
     1561 aggctgaagc catgagaagg tatgtgcgtg atgatgtgat gcagcactat gcggaagggt
     1621 tcggaaaagg cctgaacaaa gaggatatcg agtattacgg aaagatccat tttgagagga
     1681 agggagctga ccggtacgac atgcacgcac atatcatcgt cagccggaag gacaggagca
     1741 acaccaggaa gctaagtcca aagaccaacc acaccgggaa gaagaactgc ggaaacgtga
     1801 agggcggttt cgacagaaca gattttttcc gaaagtgtga atcttcattt gacaagcgca
     1861 cagggtatga ccgggccccg gaacagactt tcgactatct caataccatg aagaacggaa
     1921 gcccgaaaga aatattccag aaaaaggagt gggcggaacg tgtgaaccat gaacggcttg
     1981 agaagatgaa agccgaatgg agccgggatc ttcaggaacc gcatcaggaa caaggccgtg
     2041 aagagagcca acaacaggga aacagtattt cccaagttcc ggatatcaac caggcaccgc
     2101 agcggaagaa gcaacaggag gaagaacttg accagcccag gaaaagaagc cggggggttg
     2161 gaatgggtat gtaattttag ataacttacc gcttggtaag tgcttacctt ctgcatctaa
     2221 gaaaacagaa ggtaagcatt ctgattgatg tttttccctt aatataaatc tccttttatg
     2281 gttgtaaatg tatatgtttc tgtttctaag tccaaaatta tttggactgt tatattttct
     2341 tttgaaggat atccattggg gtatcctcca aaataatgag tggcattaaa gtttatatta
     2401 tatgtatatc catcatggta agttattttg ggttctccaa taactctata gtcaggatat
     2461 tctttcatct tttcatgaaa ttgttgtatt ataaagttgt catttaaata aagttcctct
     2521 ttttgcttga ttctttgttc tctttcaaat tctttctcta atggtgttgt ttcgtgctca
     2581 aaacttaaat ctatttctcc gttttctttc tgaggataat attctatttt tcttgttgct
     2641 atagcaatga gttcatgtag actattgaat atttctctct ttgtccaatt tcctctttca
     2701 tcataagtat attgggcgta agtttcatgt tggttatttt cagtactatc tacatctaat
     2761 atatgtacta ggtctccgtt atcattcctt tcatatgttg tcaaaaggtt gttgtcgtat
     2821 tttacatagt tataagattt tttttgtttc catgttttta tatcttccaa gatgtatgta
     2881 ggtatagtat cggttattac atttagatct ttgatgtgtt tagatgtaat ctgaccattc
     2941 tcattaattt catatttttc taataaaatc cttttccctt gtcttatttc aatctctgtg
     3001 tatttattat taccattatt tttgaataac attattgtcg tatcatttat tgctttgcca
     3061 gaaaaaggat cttttttata ttctacttta attgttttag tcttataatt atttttatct
     3121 ctttctataa tagtctttgc attaagactg ttttcattac cataatactc ttctatctta
     3181 aaaggaaaaa cattctcatc cttataatag tattttttat agtctgagac atttataaaa
     3241 tctgtttctt ctgataatgt tattatatct ttagaatgtt tttctacaat caatctacct
     3301 gagttatcat atgaataact agtaatttct tttcccttga tttctttgag caaatttttg
     3361 tgatgattaa acttaaaaat agttgtatta gagctagttt ttctgccatt ctgaatatca
     3421 aaatcttctt gtataattag acaaacattg ctctttaaat tcatattgaa gtgatttaaa
     3481 ctttttgttt tcgtgaaatt ttcttcagta acaagactgc aaatgccaag taattccaag
     3541 acatccaact tgaaacagtt gtatcgaatc ggttcagtag cgtcctgaag ctgtccatcc
     3601 aagcgtaggt tcgttcaatg gaataccttt cagcatacag ttcatcatca acaaagatgt
     3661 catcggcatt ttctctccgt ttattgatac agatgtttgc gatgacctct ttttcacaca
     3721 ggaaaccacg taagacatca caatcgaatc cggcatcggc attgaggaaa aggccatcaa
     3781 cccgaatatg ggatttctca aggtcgttga acatgtcacc gatgaccttt tctatgtcat
     3841 gtacgtcatg gtgttcgcca gatttcggag aagacatggc aatcggaagt ccttgcctgt
     3901 ctgtcaggta gagtgcgttg gtcgtcttcc gtttcttccg accctggtat ccgacttctt
     3961 caccgccacg gagtgcagcc gtgtggctcc cgtccaaatc cacgctggac atgtccaact
     4021 catggcggtg tttgtcaagc aacctgaccc acatggattt ccactcgccc gacttgctcc
     4081 atttgttgta atggtgatac acggcaccat agctcagtac cttgccgcta aacagggctt
     4141 ccacaggaag gaacgcccat tgacaaccgg tcttcaactt gtacaagatg gcgtttacca
     4201 cctctatgag acaactttga gtgacataac cacgttttgc cttggaaaga tgaggcaata
     4261 tttcattttt tatcgtatct ttgtcgagca cagtgtacat tggactttca ctatatatga
     4321 gtttggtcac ccataatata aggaacaatt caatttgtac gctgtgcttt aacattttga
     4381 tacgatttta agagaatgaa ataagtttaa atcacttcaa tttcaatttt tgaattatgt
     4441 atttcagcag accttggact tctatctaag tgataggcag tcctgaaaat aattagtcta
     4501 tgggtttcgt gtgccgactt ttttgtaaag gcgttacgtt gtcatagttg ctctgtcggg
     4561 taatactgtc tattcacttc tccacgattt gaattcaagt attcacctgt aagaagttac
     4621 taatgacaaa aaagaaattg cccgttcgtt ttacgggtca gcactttact attgataaag
     4681 tgctaataaa agatgcaata agacaagcaa atataagtaa tcaggatacg gttttagata
     4741 ttggggcagg caaggggttt cttactgttc atttattaaa aatcgccaac aatgttgttg
     4801 ctattgaaaa cgacacagct ttggttgaac atttacgaaa attattttct gatgcccgaa
     4861 atgttcaagt tgtcggttgt gattttagga attttgcagt tccgaaattt cctttcaaag
     4921 tggtgtcaaa tattccttat ggcattactt ccgatatttt caaaatcctg atgtttgaga
     4981 gtcttggaaa ttttctggga ggttccattg tccttcagtt agaacctaca aaaaagttat
     5041 tttcgaggaa gctttacaat ccatataccg ttttctatca tacttttttt gatttgaaac
     5101 ttgtctatga ggtaggtcct gaaagtttct tgccaccgcc aactgtcaaa tcagccctgt
     5161 taaacattaa aagaaaacac ttattttttg attttaagtt taaagccaaa tacttagcat
     5221 ttatttcctg tctgttagag aaacctgatt tatctgtaaa aacagcttta aagtcgattt
     5281 tcaggaaaag tcaggtcagg tcaatttcgg aaaaattcgg tttaaatctt aatgctcaaa
     5341 ttgtttgttt gtctccaagt caatgggtaa actgtttttt ggaaatgctg gaagttgtcc
     5401 ctgaaaaatt tcatccttcg tagttcaaag tcgggtggtt gtcaagatga tttttttggt
     5461 ttggtgtcgt cttttaagct gccgcataat ggttcggaaa taagcaccgt ttaggtgctt
     5521 catttctttg ttcaaggcag ttttacccac catgcactat tacatattta tatagaactt
     5581 caatttttga ctgctgtttc cggcagaaag gggtgtgaag ttcatgcctg cacccggttc
     5641 gttttattac agctcttatc cgtatttcat tcattttttt gttctccttt cttcattatc
     5701 acagtctgtc gtgtccttta aaggcttaat atttttacat gagcttataa aaaataatat
     5761 agcaaaaata ttacatgcca ctaaaattct ctgattcatc ctttccataa ttctgtattt
     5821 acttttattt ttccgaatgt tttttgttca tcacgtaccc ggcacagttc ccggtatgct
     5881 ttttccttat actcctgaag aactttgatt tcgttacgca atcggttgtt ttcttcctga
     5941 agaacctgaa gatcttttgc ctgttcttca gctttcattt cagcacgtac tttctcttca
     6001 gctgcctttg cgataagttc cggagccgga aactgtatct gcttttgtgc cgttataaat
     6061 tcgctcatga gcatacacaa ggcttctact tggtttcttt ccttctcgat gtctggagaa
     6121 acaggaagaa atggtgttgt ttccttgtca tagatgtcga agtcgttctt ttctatgaat
     6181 ttccaggctg tttcagccca ttcaccgaca ggcttccctg cttttaggca atatattttt
     6241 atttcttcga gtatgttttt aggtactctt agagttgatg tctgtatatt tgttttttct
     6301 tgcatgtcaa tgatttatgt gcaaaaatat aaagaatata tttagtaata tacatattct
     6361 acaaaaaata tattattgct ttgttatgtg tacatctaca aaacaaccac cgaatccaga
     6421 taaggcgtct ggttgcgtat aagttactgt tacaagggtt tgtcccatga atgtctcaat
     6481 cttgtctatg acggcttcca ttggcttgcc taagcctgta gcttcagcag ataccaaaat
     6541 caaatctcct tctttcatgt ctttgtttat tttgattgtg tgacaaaatc aaatcttctt
     6601 tctatcttta atctttccct tgatagcatt gattatataa ccttttgggt ttttcttttc
     6661 ccttgatttc ccttttagaa tggcgagttc attaaggaag tccggaagta aggtttgtgc
     6721 tgatataaac agatccagat tgttcttaat ctctttatct gaaaactcaa tcgagttttt
     6781 aagataactt cttatttccg gagttaacga ccagcttaaa gctgtctgtt tttgaaggtc
     6841 gtgtttttcc agatcgctat cacgatgttc tggttgatat accggataaa actttatggc
     6901 cgttatcttt cgtcctgttt tgagcggtgt gtactcaaag gtataagggc tgacttcatc
     6961 aagctctttt tttgcagctt ctactacttt gcgcatgaaa tcatttattc gctcatactt
     7021 atctgccaca caaaacattt cctttagctg ttctatagag tatatcagag gagttttctg
     7081 gttgctcagc aactcataaa agcgcattga aaactggctc ttaaagctca tggcagcttt
     7141 caattcgtat tttctgaagc ctttggagaa atcaagtatg cagtcccata cacgtgggtg
     7201 cacctgaaag gacaagactg aagaacgttt tcgtatataa ggcaaggcta tgatagatat
     7261 actttgccag atttcatcat cttcatactc aaagatcttt cgctgaagcg actgtagggc
     7321 ttcttttact cttgcataat ttttgtcatc ctctccatta agcaggcagg caataggcat
     7381 ggttatattt acgcaactga aaagatcatg ctctatcttc tgacagttat cagcgaactt
     7441 tataccttgc agctctcctt gagccatttc tacaagacga taaatgatac gcttttcgta
     7501 cacgttaaag tcatattttg cagtagtcat aatataactt tggataaggt ctttgtttac
     7561 tattgccata attatcagtg ttttgcgcaa atgtaagtat taaaaacgac tttaacaaat
     7621 aaaagtcgca gattatgtgg ttagtattca aaaagtcgta gttaaacttt gaaatagtat
     7681 tcgaaaagtc gtagttagta ttcaaaaagt cgtagttaga ttcttgttgt tgtttataat
     7741 tcattgttta ttagattgtt aaaagatgtt tttcaaaagt tgataagcat ataacaagta
     7801 atgcttaaaa acaagtcata aaattttagt cagaatgtgg cttccaaacc tccgaatccg
     7861 cgaaaaacga ctttttgaat gtttgtgaaa gcaaagcaaa agccagcgca gcgttttttg
     7921 aggaacgaat tttccgcgcg tgtacacaaa taagtacata aaacttttgt tttattgatt
     7981 tttatcccta tttttgcggt gtaaaccaat atattagaac tatgcagatt gttacaacac
     8041 gcgaatttcg tgccaaccag aagaaatact tcgaaatggc agagacggaa acgatcctga
     8101 tctccagacg caatgcggca cctatcatgg tgtgtgcggt cagggaaggc gattttcctt
     8161 caagagaaga gctggcggcc attcagaggg gtattgatga tgtcaggaac ggaaatacct
     8221 tcagaatggc taaaaacgag tctctggacg attttcttaa ccgtatagag ggtgaaggca
     8281 atgtatgaga tagaattttc caaagaagcg gtaaaacatg tcctgtta
//
