LOCUS       CP103258                8252 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-526 plasmid unnamed1, complete
            sequence.
ACCESSION   CP103258
VERSION     CP103258.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323426
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8252)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 29x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 22:04:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,812
            CDSs (total)                      :: 4,720
            Genes (coding)                    :: 4,479
            CDSs (with protein)               :: 4,479
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 241
            CDSs (without protein)            :: 241
            Pseudo Genes (ambiguous residues) :: 0 of 241
            Pseudo Genes (frameshifted)       :: 186 of 241
            Pseudo Genes (incomplete)         :: 64 of 241
            Pseudo Genes (internal stop)      :: 11 of 241
            Pseudo Genes (multiple problems)  :: 17 of 241
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-526"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="2009-08-31"
     gene            1..462
                     /locus_tag="NXV60_00005"
     CDS             1..462
                     /locus_tag="NXV60_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS37329.1"
                     /translation="MNRIIHISLLISTVSLFLISNTGCVEKQGYYNHGEESIISLICDI
                     TWAGKKTTDENGSVWQGTYKFNKNGTYTRTNIEIDKQGNKKEANIYGQWSFGDPSFSTI
                     YFGGEHYWDIDELTKNKFSFYDRSGKFGDPFMNREYIELTPYQENNTTN"
                     /name="hypothetical protein"
     gene            complement(466..804)
                     /locus_tag="NXV60_00010"
     CDS             complement(466..804)
                     /locus_tag="NXV60_00010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS37330.1"
                     /translation="MTVKKKIQINYKALDMIYTAMHGMQRMSSFGQFRTMIGDEALNDF
                     RDEDLVLNIEGLNVEDGCYTWYMSVKDKVADWYNMETGEALCCYKVTRKDELLQDIYLD
                     EDIFPNSG"
                     /name="hypothetical protein"
     gene            complement(770..1369)
                     /locus_tag="NXV60_00015"
     CDS             complement(770..1369)
                     /locus_tag="NXV60_00015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="UVS37331.1"
                     /translation="MKAYMDAHVCSNVYTVQGGKAHSHYCHRAFCPICQRIQTAQNVKK
                     FLPVMKYLASEGREFYFVTLTLQNCVTDDARVLRDFMRRCNRMWADGIRTKHKFRSLGI
                     SGVIKKECTYHVVKKDLFSFHYHFHIIVDSLEAAKYVVAQWKKLHGSTVADARFQKYKK
                     IEDFENAAIEVFKYASKASVSKSKGRDGKKKDSDKL"
                     /name="protein rep"
     gene            complement(2011..2409)
                     /locus_tag="NXV60_00020"
     CDS             complement(2011..2409)
                     /locus_tag="NXV60_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-poly-alpha-D-galacturonosidase"
                     /protein_id="UVS37332.1"
                     /translation="MKPTDYIEWDNLKDIPFFLCQVVEDREKQDLDIYYLGKRVLHDYD
                     HVGHYLRTAVILFRRVKSRTADWVNLRNLWTLRNCVRENYNHGIGMNDLIFGENFDGDN
                     LDTLTPLTKKRFDFLCKRIKELDPYATI"
                     /name="exo-poly-alpha-D-galacturonosidase"
     gene            complement(2446..2766)
                     /locus_tag="NXV60_00025"
     CDS             complement(2446..2766)
                     /locus_tag="NXV60_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="UVS37335.1"
                     /translation="MVEQYEVYWVELDPTRGGEMAKTRPCVVVTPSDLNMYLTTVVIVP
                     ITSTIRNYPYRVLCSVAGREGEIATDQIRTVDKSRLKRKIGDLNFSEIKRLREVFQQMF
                     CE"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(2760..3008)
                     /locus_tag="NXV60_00030"
     CDS             complement(2760..3008)
                     /locus_tag="NXV60_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="UVS37333.1"
                     /translation="MVTNIIQIGNSKGIILPSEVLKQLRLSLKSAVSISLDGNNIVIKA
                     QPRQGWAEAAKRAHENGDDELLIPDVFEDEKFEDWTW"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            3406..4593
                     /locus_tag="NXV60_00035"
     CDS             3406..4593
                     /locus_tag="NXV60_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="UVS37334.1"
                     /translation="MGATSIHVQAVKPGSEIHNFREKELDYVRPELSHLNESWVGDSIS
                     HRLESAKQRYFDTVGQKMQTKAAPIREGVIVIKQETTMQELQQFAAVCKERFGIEAFQI
                     HIHKDEGYMNAKQWTPNLHAHVVFDWTQPNGKSVRLSRDDMAELQTIASEALGMERGVS
                     SDRKHLSAMQYKTECAKEQLQELSNDISSALDKHKDVQNQLLQLQKELRSIETKKNVQK
                     LISKASEKFYGLIGKTVNDREKDALKAKIKALEGENEQLSDRLGKAILEKERNGTKVFK
                     AENDKEYYRQQMDNARTTSNRLRTENQELKTETKELKKELGKMKDLFNSEQLEALRHHF
                     PNISVTPFGFGRNFAGFEKRLCAYLKKSLWCGRSSGRVVPPLAAGRSPTDDFLSS"
                     /name="mobilization protein"
     gene            4855..5445
                     /locus_tag="NXV60_00040"
     CDS             4855..5445
                     /locus_tag="NXV60_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA protein"
                     /protein_id="UVS37326.1"
                     /translation="MENKKVLKLTDFQKNEENPFMKQAIEGIENHVVKKYKSSTGSDKK
                     AVVAVADTDTGEVFKTSFIRQIEVDEEQFAKLYLANFAAFFNLSQSAIRVFGYILTCMK
                     PKNDMIIFDRRKCLEYTQYKSDKAIYKGLAELVQSEIIARGPNEYNWFINPLIVFNGDR
                     VSFTKTYVRKKTLAAQKKEEAEKRQLSLGFDEL"
                     /name="RepA protein"
     gene            6071..7321
                     /locus_tag="NXV60_00045"
     CDS             6071..7321
                     /locus_tag="NXV60_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS37327.1"
                     /translation="MAKTCIRVEACNIGSSERHNLRSKELDYIRPELTHRNEQWVECSI
                     AEVHRDITEKYKEATGQGLQKKATPIREGVIVISEETTIQQLQDLAEKLEERFGVHAFQ
                     IYTHKDEGANVWDGKEEAWKPNYHAHMIFDWTDGHTGKTVKLNRHDMAEMQTITAECLN
                     MERGVSSDKKHLSAMQYKNKMETEKAEQLQKDIEQLNRAYTAGTEKITTVQKELSTAQK
                     ELNSMKTDIHINEAKEAAAKAGKTVFNAVTSVFNVGEVKRQAKEIETLKKENYNLSFKN
                     QNLEGQLRTNNIEMRRVQETTDRQIKAEASKLKPITNLFPEMENAQENIEELQTMGIQN
                     KDIRQLLIGKEIYYTGNLYDKDKRKSYQVKNVKIDISKSQNGITTIWLNNIHFKNFLKE
                     LWNTLQKTLDNGNWLHR"
                     /name="hypothetical protein"
     gene            complement(7391..8161)
                     /locus_tag="NXV60_00050"
     CDS             complement(7391..8161)
                     /locus_tag="NXV60_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS37328.1"
                     /translation="MKKVMFMFISLLTVLGVCSCNDNEVIEPVVSDSVSDMEVLSRFVD
                     VNEITNEYYFNENKKTRALSYVTGSDWQDLEKVSPLSIEKYKNNLQVLNAQVASAISNP
                     NTAYVVFSVNGKTLVKKVKEDANFDFSVFRDVVTETRAVLPSLSINGGSQSTTGVFYDS
                     SRTLKMQVDLNASIQNNYYFFEVLNPNAKPSPDDNITTPESVAFSGTGPLWSNTFTWTS
                     YWDANVPGQGFKWEFKGKGTTPSFGFIANCTFSR"
                     /name="hypothetical protein"
     Typing          complement(467..1516)
                     /name="rep_cluster_870"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.715"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4856..5435
                     /name="rep_cluster_1868"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.479"
                     /coverage="95.0"
                     /evalue="2.71e-121"
     Typing          3407..4437
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.93"
                     /coverage="94.0"
                     /evalue="0.0"
     Typing          6072..7318
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaatagaa taatacatat ctctttactt atttcaacag tatcactttt tcttatttca
       61 aacaccggat gtgttgaaaa acaaggatac tacaaccatg gagaagaaag cataatctca
      121 ttaatttgtg atattacatg ggctggtaaa aaaacaacag acgaaaacgg cagtgtctgg
      181 caaggtacat acaaattcaa taaaaacgga acatataccc gtacaaatat agaaatcgac
      241 aaacaaggaa ataaaaaaga agccaatatc tacggacaat ggtcattcgg tgatccaagt
      301 ttcagtacca tttattttgg tggagaacat tactgggaca tagacgagct aactaaaaac
      361 aaattttcct tctatgacag aagtggaaaa tttggcgatc ctttcatgaa tagagaatac
      421 atagaactca caccatacca agaaaacaat acaacaaact aacaactacc cactattagg
      481 aaaaatatcc tcatctaaat aaatatcctg caaaagttca tccttccggg tgaccttata
      541 acaacacaat gcctccccag tttccatatt ataccaatca gccaccttat ctttcacaga
      601 catataccaa gtataacaac catcttcaac attcaatccc tcaatattaa gaaccaaatc
      661 ctcatctcta aaatcattca acgcttcatc tccgatcatg gtgcggaact gtccgaaact
      721 actcatgcgc tgcatcccat gcatggcagt ataaatcata tccaaagcct tataatttat
      781 ctgaatcttt ttttttaccg tcacgacctt tcgattttga gacagaagcc tttgaagcat
      841 acttaaaaac ctctatcgct gcattttcaa aatcctctat cttcttatat ttctgaaaac
      901 gggcatcagc aaccgtacta ccatgcaatt tcttccattg ggctactaca tattttgcag
      961 cttccaatga atcaacaata atatgaaaat gataatggaa cgaaaacaaa tcctttttta
     1021 ctacatgata ggtacactcc ttcttaatca cccccgaaat accaagtgaa cgaaacttgt
     1081 gtttcgtcct tataccatca gcccacatcc ggttacatct acgcatgaaa tctcgcaata
     1141 cccttgcatc atcggttaca caattttgca aggtaagcgt cacaaaataa aactctctgc
     1201 cttccgaagc caaatacttc ataaccggaa ggaacttctt tacattttgt gccgtttgaa
     1261 tacgctgaca gataggacaa aaagcccgat gacaataatg ggaatgcgcc ttacctccct
     1321 gcacagtata aacatttgaa cacacatggg catccatata tgctttcatc agcttactat
     1381 ccggattctt ttccgaaagt tttcgagtaa aattatggga tatgattttt actttagcct
     1441 tttttttaag actatcagaa ccgtttttta acggactttc tttgcagttg gttatatttg
     1501 ttctatcttt gcacatatga aattttgatg tggctttcgt ttggtatccg ccccgccaag
     1561 aacgaacaca aacgattgct aattcttaaa atacaaagcc cgttggttac tgccttcggg
     1621 ctttcgtttt ttctcagccc aaaagtaggt aattcattcc aaaccgccaa acatcattca
     1681 aaaaacctac atataacaag tcaaaaagca tttaccacga aactttctag tttatctagt
     1741 atgggggctc gcccccattc ccccggctgc gcaattctga cacaaataca ctactaaatc
     1801 cacaacagac aaagatactt ccggaaaaaa aactatcttt gccgtacaca aagacaaccc
     1861 ttgccgagcg atacgggaga gttgcaaaaa aaacaccgga gtgaattgac ggatatccga
     1921 catctccggt ggtatttttt ttcttatccc cctcccgtcc cccaaagggg gagcacacaa
     1981 agacaagccc tgccttcact caatggagtg ttaaatagtt gcatatgggt caagttcctt
     2041 aatccgctta cacaaaaaat caaagcgttt ctttgtaagt ggtgtaagcg tatcgagatt
     2101 atctccatcg aaattctccc caaaaataag gtcgttcatt cctatgccgt gattgtagtt
     2161 ctcacgcaca cagttgcgca gcgtccaaag gttacgcagg tttacccagt ctgctgtgcg
     2221 gctctttacc ctgcggaaca agataacggc agtccgcaaa tagtgcccca catggtcgta
     2281 atcgtgcaag acacgtttgc ccaaatagta aatatccaaa tcttgttttt ctctgtcctc
     2341 gactacttga cagaggaaga atggaatatc cttcaggtta tcccattcga tgtagtcagt
     2401 tggtttcata atgtcttatt tgcatttttc tttccgtact ttccattatt cacagaacat
     2461 ttgctgaaat acttcacgta acctttttat ctcactgaaa ttcaaatctc caatctttct
     2521 tttcagccgg cttttatcca ccgtgcgaat ttgatcagtg gctatctctc cttcacgccc
     2581 ggctacagaa cacagaaccc ggtaaggata atttcttatc gttgaggtta tcggaacaat
     2641 gactactgtt gtaaggtaca tattcaaatc tgaaggagtg acaactacac atggccgtgt
     2701 ctttgccatt tcacccccac gggtagggtc taactctacc caatacactt catactgttc
     2761 taccatgtcc aatcctcaaa cttttcatcc tcaaacacat cagggataag caactcatca
     2821 tccccattct catgggcacg tttggcagct tccgcccatc cctgccgtgg ttgtgccttt
     2881 atcacaatat tgttcccgtc caaagaaatg ctcacagccg atttcaagga caaacgcaac
     2941 tgtttcaata cttcggaagg aagtattatc cccttgctgt ttccaatctg aataatattc
     3001 gttaccataa tgttataact ttataattga ttatcacgat acaaatatat gtaaagttat
     3061 aacaataaca aaaggaaaaa caactttttt ccattttcac cgagaaccct gtcaatggct
     3121 gcactcgtcg ggagtgttgg tgcaccccct tgccgtgcag cgctccccga ccgatgattt
     3181 tttattgtgc cttgtgtcct tttccctttt gtccggcttc tgttcccaag cacatccgga
     3241 acacatggca gagcgcttgc aaatcccgtc cggaaggctt acctttgcaa aaggttatag
     3301 cacactagca ccctaaaggg tacgtgtgta aggggacaag cccccttaac cccctgtcag
     3361 ccggctgtca ccggctgtta tcgattgtca caaaaatgtt aacttatggg agcaacaagt
     3421 attcatgtac aagcagtgaa gccggggagc gagattcaca actttaggga aaaagagttg
     3481 gactatgttc gtccggaact tagtcatttg aatgaaagct gggttggaga tagcatctcc
     3541 catcggctgg agagtgcgaa acagagatat ttcgatacgg ttgggcagaa gatgcagact
     3601 aaagcagcac ccatacggga gggggtaata gtaatcaagc aagaaaccac catgcaggaa
     3661 ctccagcaat tcgctgcggt ctgcaaggaa cgtttcggta ttgaagcgtt tcaaatccat
     3721 atacacaagg acgaaggata catgaacgca aagcaatgga cacctaacct tcacgcccat
     3781 gtagttttcg attggacgca gccgaacggg aagagtgtgc gtttatcgcg tgatgacatg
     3841 gcagaactcc aaaccatagc atctgaagca ctgggcatgg aacgtggtgt ttcttctgat
     3901 cgcaaacact tatcggctat gcagtacaag accgaatgtg cgaaagaaca gcttcaggaa
     3961 ctatccaacg atatatccag tgcgttagac aagcacaagg acgtgcaaaa ccagcttctt
     4021 cagcttcaga aagaactacg ttccattgaa acaaagaaaa acgtccaaaa actcatttct
     4081 aaggcttcag agaagtttta cggcttgata gggaaaacgg ttaacgatag ggaaaaagat
     4141 gccttaaaag ccaaaataaa ggctttagag ggggaaaatg aacagctatc cgatagactg
     4201 ggaaaggcta tacttgaaaa agagcgaaac ggcacaaagg tattcaaagc cgagaacgac
     4261 aaggagtatt atagacagca aatggacaat gcaaggacaa ccagtaaccg actaagaacg
     4321 gagaaccaag aactaaagac cgaaaccaag gaattgaaaa aagaacttgg taagatgaaa
     4381 gacttgttca attccgaaca actggaggct ttaaggcatc atttcccaaa catatcagtt
     4441 acgccgttcg ggttcggaag aaattttgca gggttcgaaa aacggttgtg tgcttatttg
     4501 aaaaaaagtt tatggtgtgg gcgctcgtcg ggacgggtgg tcccgcccct tgccgctggg
     4561 cgctccccga ccgatgattt tttaagtagt tgattatatg ccgtttttag ggtataataa
     4621 gaacttaaga aatagaataa gttaagtagt tgatactcaa tatagggtat tttccatatt
     4681 ggaaaatctg attttccata ttagaaagtc gctctttcct attagatgtt aaaaaggaaa
     4741 atatattttg cttttttatt gtattaatgg aaatttaaat attcctttgt ggaaaatcaa
     4801 attatacttt ttcaccgata tatagaaagt caaatttgcc taaatagaat gtttatggaa
     4861 aataagaaag tactcaaatt aaccgatttt caaaaaaacg aagaaaaccc ctttatgaaa
     4921 caagcaattg aagggattga aaatcacgtt gttaagaaat acaaaagtag tacgggaagt
     4981 gataaaaaag ctgtagttgc tgttgctgat acagatacag gagaagtatt caaaacctcg
     5041 tttatccgac aaatagaagt agatgaagag caatttgcaa aactatacct tgcgaatttt
     5101 gccgcttttt tcaatctatc acaatcagct attcgggttt tcgggtatat tctaacttgc
     5161 atgaagccta agaatgatat gataattttc gataggcgaa aatgcttaga atacacccaa
     5221 tacaaatcag acaaagccat atacaaagga ttggcagagc tcgtacaaag tgaaatcata
     5281 gcaagaggtc caaacgaata taattggttc attaatccgt tgattgtctt taatggggat
     5341 agggtttcat ttacaaaaac atacgttcgg aaaaagactt tagctgccca aaagaaagaa
     5401 gaagcagaga aacgacagtt atcacttggt tttgatgaac tgtaacactc cattgagtga
     5461 agctgccgtt tggtcgctcc ccctttgggg gcgggggggg atagataaag ttcctctatg
     5521 taaagttata atgggggatg aaaggcaagg ttcgctaacc ttaccccgat tgtttatgct
     5581 tcccagcatt caatctatcc ccgtttatgg gtttctcttt caccctacaa agataacctc
     5641 atgggggaaa aatgtccaag aatatggggt aaaaactatc aagtcggtag aaaataagta
     5701 tctttgaagt acatttttag tagaggtact ggtatgccta gacgaaacag ataggcaaaa
     5761 ataaagccgc tggggatgtc ggatgtccgt caattaactc cagcggcttg tattttggta
     5821 actctcccgt atcggtcagc taccgtttgg tcagcaacaa aggtagtact ttattgtcgc
     5881 aaatccaaat gttttcccct ttatttgtgc atttacaaaa cattcactaa cttcgcacct
     5941 atgcagtatg gtgatagcac actataaccc ctaaaggggt tgtgtgcgag gggtgtcccc
     6001 tcgaccccgt gcgatgtgac gtttcacgcc acatcttcta aacagacata cacacctaaa
     6061 acctaaagat atggcaaaga catgtatcag agtggaagcc tgcaatatag gcagctcgga
     6121 acgtcacaac ttaagaagca aggagctcga ctacatccgt ccggaactca cacaccgcaa
     6181 cgaacagtgg gtagaatgtt ccattgccga agtacatcgg gacataacgg aaaaatacaa
     6241 ggaagccacc ggacaaggat tacagaagaa agctacccca atccgggaag gggtaatagt
     6301 aatcagcgag gaaaccacaa tccagcagct tcaagacttg gccgaaaaac tggaagaacg
     6361 ctttggagtg cacgccttcc aaatctacac ccataaggat gaaggcgcaa acgtatggga
     6421 cggaaaggaa gaagcatgga aaccgaacta tcatgcgcac atgatctttg actggacgga
     6481 cggccataca ggaaaaactg taaagctgaa cagacacgac atggcggaga tgcagactat
     6541 aacagcagag tgtctcaaca tggaacgtgg agtttcttca gacaagaaac acttgtcggc
     6601 catgcaatac aaaaacaaaa tggaaacaga gaaagctgaa caacttcaaa aagacatcga
     6661 gcaactaaac agggcataca ctgccggaac cgagaaaatc accacagtcc aaaaggaact
     6721 tagtacggca caaaaagagc taaacagcat gaaaaccgac atacacatca acgaagccaa
     6781 agaagctgcc gcaaaagccg gaaaaaccgt atttaatgcc gttacaagcg tctttaatgt
     6841 aggggaagta aaacgacaag ccaaagaaat tgaaaccctc aaaaaagaaa attataacct
     6901 ctcattcaag aatcaaaatt tagaaggaca attaagaacc aacaacatcg agatgcgaag
     6961 ggtacaggaa acaaccgaca gacagataaa ggcagaagca tccaaactaa aacccataac
     7021 caaccttttt ccggaaatgg aaaatgcaca ggaaaatata gaggaactcc aaactatggg
     7081 aatacaaaat aaagatatcc gacaacttct tattggaaag gaaatatact acactggaaa
     7141 cctttacgat aaagacaaga gaaaatcata ccaagttaaa aacgtgaaaa tagatatctc
     7201 caaaagtcag aatggtatta ctacaatttg gctaaataac atccacttta aaaactttct
     7261 taaagaactt tggaatacac tacaaaagac tttagataac ggtaactggc tacaccgata
     7321 gaaagacact aatttgaatt gcacttctct tttttgagaa gtgcaattca aattaaatcc
     7381 cgttcatttt ttaacgacta aaggtgcaat tagctataaa accaaaagaa ggagtagtac
     7441 cttttccttt aaactcccat ttaaaacctt gaccaggtac atttgcatcc caatacgaag
     7501 tccaagtgaa agtgttactc cataacggtc cagttccact aaaagcaaca ctttcaggtg
     7561 tcgttatatt atcatccgga cttggtttag cattaggatt taatacttca aagaaataat
     7621 aattattctg tattgaagca ttcaaatcaa cttgcatctt taaagtacga ctggaatcgt
     7681 aaaaaactcc ggtagtgctt tgagaacctc cattaatact caaagaaggg agaacagccc
     7741 tcgtttccgt tacaacatcc ctaaatactg aaaaatcaaa attagcatcc tccttcacct
     7801 ttttaaccaa agtctttcca tttacagaaa aaacaacata agcggtatta ggatttgaaa
     7861 tagcacttgc cacttgagca tttaaaacct gcaggttatt cttatatttc tcaatactca
     7921 aaggactaac tttttctaaa tcctgccaat ccgaacctgt tacataagac aacgccctcg
     7981 ttttcttatt ctcattgaaa tagtattcat ttgtaatctc atttacatca acaaaacgag
     8041 ataagacctc catatcagat acagaatcac taaccacagg ttctattact tcattatcat
     8101 tacacgaaca aacacccaaa acagtcagta gactgatgaa cataaacatt acttttttca
     8161 taaaccctcc ttttttaaag ttaataaata aaggtttcgc ccaaaaatat aaaatttctc
     8221 ctatttttgc attgaatatt aaatatttaa ct
//
