LOCUS       CP103254                4633 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-506 plasmid unnamed1, complete
            sequence.
ACCESSION   CP103254
VERSION     CP103254.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323409
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 118x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 21:53:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,531
            CDSs (total)                      :: 4,438
            Genes (coding)                    :: 4,127
            CDSs (with protein)               :: 4,127
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 311
            CDSs (without protein)            :: 311
            Pseudo Genes (ambiguous residues) :: 0 of 311
            Pseudo Genes (frameshifted)       :: 262 of 311
            Pseudo Genes (incomplete)         :: 55 of 311
            Pseudo Genes (internal stop)      :: 14 of 311
            Pseudo Genes (multiple problems)  :: 16 of 311
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-506"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="2004-04-14"
     gene            complement(252..1502)
                     /locus_tag="NXV41_22600"
     CDS             complement(252..1502)
                     /locus_tag="NXV41_22600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS23350.1"
                     /translation="MAKTCIRVEACNIGSSERHNLRSKELDYIRPELTHRNEQWVECSI
                     AEVHRDITEKYKEATGQGLQKKATPIREGVIVISEETTIQQLQDLAEKLEERFGVHAFQ
                     IYTHKDEGANVWDGKEEAWKPNYHAHMIFDWTDGHTGKTVKLNRHDMAEMQTITAECLN
                     MERGVSSDKKHLSAMQYKNKMETEKAEQLQKDIEQLNRAYTAGTEKITTVQKELSTAQK
                     ELNSMKTDIHINEAKEAAAKAGKTVFNAVTSVFNVGEVKRQAKEIETLKKENYNLSFKN
                     QNLEGQLRTNNIEMRRVQETTDRQIKAEASKLKPITNLFPEMENAQENIEELQTMGIQN
                     KDIRQLLIGKEIYYTGNLYDKDKRKSYQVKNVKIDISKSQNGITTIWLNNIHFKNFLKE
                     LWNTLQKTLDNGNWLHR"
                     /name="hypothetical protein"
     gene            1908..2156
                     /locus_tag="NXV41_22605"
     CDS             1908..2156
                     /locus_tag="NXV41_22605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="UVS23351.1"
                     /translation="MVTNIIQIGNSKGIILPSEVLKQLRLSLKSAVSISLDGNNIVIKA
                     QPRQGWAEAAKRAHENGDDELLIPDVFEDEKFEDWTW"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            2150..2470
                     /locus_tag="NXV41_22610"
     CDS             2150..2470
                     /locus_tag="NXV41_22610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="UVS23352.1"
                     /translation="MVEQYEVYWVELDPTRGGEMAKTRPCVVVTPSDLNMYLTTVVIVP
                     ITSTIRNYPYRVLCSVAGREGEIATDQIRTVDKSRLKRKIGDLNFSEIKRLREVFQQMF
                     CE"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            2507..2905
                     /locus_tag="NXV41_22615"
     CDS             2507..2905
                     /locus_tag="NXV41_22615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-poly-alpha-D-galacturonosidase"
                     /protein_id="UVS23348.1"
                     /translation="MKPTDYIEWDNLKDIPFFLCQVVEDREKQDLDIYYLGKRVLHDYD
                     HVGHYLRTAVILFRRVKSRTADWVNLRNLWTLRNCVRENYNHGIGMNDLIFGENFDGDN
                     LDTLTPLTKKRFDFLCKRIKELDPYATI"
                     /name="exo-poly-alpha-D-galacturonosidase"
     gene            3547..4449
                     /locus_tag="NXV41_22620"
     CDS             3547..4449
                     /locus_tag="NXV41_22620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="UVS23349.1"
                     /translation="MKAYMDAHVCSNVYTVQGGKAHSHYCHRAFCPICQRIQTAQNVKK
                     FLPVMKYLASEGREFYFVTLTLQNCVTDDARVLRDFMRRCNRMWADGIRTKHKFRSLGI
                     SGVIKKECTYHVVKRDLFSFHYHFHIIVDSLEAAKYVVAQWKKLHGSTVADARFQKYKK
                     IEDFENAAIEVFKYASKASVSKSKGRDGKKKIQINYKALDMIYTAMHGMQRMSSFGQFR
                     TMIGDEALNDFRDEDLVLNIEGLNVEDGCYTWYMSVKDKVADWYNMETGEALCCYKVTR
                     KDELLQDIYLDEDIFPNSG"
                     /name="protein rep"
     Typing          3401..4449
                     /name="rep_cluster_870"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.81"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(256..1502)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ataatataac gacacctgaa agtgttgctt ttagtggaac tggaccgtta tggagtaaca
       61 ctttcacttg gacttcgtat tgggatgcaa atgtacctgg tcaaggtttt aaatgggagt
      121 ttaaaggaaa aggtactact ccttcttttg gttttatagc taattgcacc tttagtcgtt
      181 aaaaaatgaa cgggatttaa tttgaattgc acttctcaaa aaagagaagt gcaattcttt
      241 ttagtgtctt tctatcggtg tagccagtta ccgttatcta aagtcttttg tagtgtattc
      301 caaagttctt taagaaagtt tttaaagtgg atgttattta gccaaattgt agtaatacca
      361 ttctgacttt tggagatatc tattttcacg tttttaactt ggtatgattt tctcttgtct
      421 ttatcgtaaa ggtttccagt gtagtatatt tcctttccaa taagaagttg tcggatatct
      481 ttattttgta ttcccatagt ttggagttcc tctatatttt cctgtgcatt ttccatttcc
      541 ggaaaaaggt tggttatggg ttttagtttg gatgcttctg cctttatctg tctgtcggtt
      601 gtttcctgta cccttcgcat ctcgatgttg ttggttctta attgtccttc taaattttga
      661 ttcttgaatg agaggttata attttctttt ttgagggttt caatttcttt ggcttgtcgt
      721 tttacttccc ctacattaaa gacgcttgta acggcattaa atacggtttt tccggctttt
      781 gcggcagctt ctttggcttc gttgatgtgt atgtcggttt tcatgctgtt tagctctttt
      841 tgtgccgtac taagttcctt ttggactgtg gtgattttct cggttccggc agtgtatgcc
      901 ctgtttagtt gctcgatgtc tttttgaagt tgttcagctt tctctgtttc cattttgttt
      961 ttgtattgca tggccgacaa gtgtttcttg tctgaagaaa ctccacgttc catgttgaga
     1021 cactctgctg ttatagtctg catctccgcc atgtcgtgtc tgttcagctt tacagttttt
     1081 cctgtatggc cgtccgtcca gtcaaagatc atgtgcgcat gatagttcgg tttccatgct
     1141 tcttcctttc cgtcccatac gtttgcgcct tcatccttat gggtgtagat ttggaaggcg
     1201 tgcactccaa agcgttcttc cagtttttcg gccaagtctt gaagctgctg gattgtggtt
     1261 tcctcgctga ttactattac cccttcccgg attggggtag ctttcttctg taatccttgt
     1321 ccggtggctt ccttgtattt ttccgttatg tcccggtgta cttcggcaat ggaacattct
     1381 acccactgtt cgttgcggtg tgtgagttcc ggacggatgt agtcgagctc cttgcttctt
     1441 aagttgtgac gttccgagct gcctatattg caggcttcca ctctgataca tgtctttgcc
     1501 atatctttag gttttaggtg tgtatgtctg tttagaagat gtggcgtgaa acgtcacatc
     1561 gcacggggtc gaggggacac ccctcgcaca caaccccttt aggggttata gtgtgctata
     1621 accttttgca aaggtaagcc ttccggacgg gatttgcaag cgctctgcca tgtgttccgg
     1681 atgtgcttgg gaacagaagc cggacaaaag ggaaaaggac acaaggcaca ataaaaaatc
     1741 atcggtcggg gagcgctgca cggcaagggg gtgcaccaac actcccgacg agtgcagcca
     1801 ttgacagggt tctcggtgaa aatggaaaaa agttgttttt ccttttgtta ttgttataac
     1861 tttacatata tttgtatcgt gataatcaat tataaagtta taacattatg gtaacgaata
     1921 ttattcagat tggaaacagc aaggggataa tacttccttc cgaagtattg aaacagttgc
     1981 gtttgtcctt gaaatcggct gtgagcattt ctttggacgg gaacaatatt gtgataaagg
     2041 cacaaccacg gcagggatgg gcggaagctg ccaaacgtgc ccatgagaat ggggatgatg
     2101 agttgcttat ccctgatgtg tttgaggatg aaaagtttga ggattggaca tggtagaaca
     2161 gtatgaagtg tattgggtag agttagaccc tacccgtggg ggtgaaatgg caaagacacg
     2221 gccatgtgta gttgtcactc cttcagattt gaatatgtac cttacaacag tagtcattgt
     2281 tccgataacc tcaacgataa gaaattatcc ttaccgggtt ctgtgttctg tagccgggcg
     2341 tgaaggagag atagccactg atcaaattcg cacggtggat aaaagccggc tgaaaagaaa
     2401 gattggagat ttgaatttca gtgagataaa aaggttacgt gaagtgtttc agcaaatgtt
     2461 ctgtgaataa tggaaagtac ggaaagaaaa atgcaaataa gacattatga aaccaactga
     2521 ctacatcgaa tgggataacc tgaaggatat tccattcttc ctctgtcaag tagtcgagga
     2581 cagagaaaaa caagatttgg atatttacta tttgggcaaa cgtgtcttgc acgattacga
     2641 ccatgtgggg cactatttgc ggactgccgt tatcttgttc cgcagggtaa agagccgcac
     2701 agcagactgg gtaaacctgc gtaacctttg gacgctgcgc aactgtgtgc gtgagaacta
     2761 caatcacggc ataggaatga acgaccttat ttttggggag aatttcgatg gagataatct
     2821 cgatacgctt acaccactta caaagaaacg ctttgatttt ttgtgtaagc ggattaagga
     2881 acttgaccca tatgcaacta tttaacactc cattgagtga aggcagggtt tgtctttgtg
     2941 tgctcccctt ttgggggacg ggagggggat aagaaaaaaa ataccaccgg agatgtcgga
     3001 tatccgtcaa ttcactccgg tgtttttttt gcaactctcc cgtatcgctc ggcaagggtt
     3061 gtctttgtgt acggcaaaga tagttttttt tccggaagta tctttgtctg ttgtggattt
     3121 agtagtgtat ttgtgtcaga attgcgcagc cgggggaatg ggggcgagcc cccatactag
     3181 ataaactaga aagtttcgtg gtaaatgctt tttgacttgt tatatgtagg ttttttgaat
     3241 gatgtttggc ggtttggaat gaattaccta cttttgggct gagaaaaaac gaaagcccga
     3301 aggcagtaac caacgggctt tgtattttaa gaattagcaa tcgtttgtgt tcgttcttgg
     3361 cggggcggat accaaacgaa agccacatca aaatttcata tgtgcaaaga tagaacaaat
     3421 ataaccaact gcaaagaaag tccgttaaaa aacggttctg atagtcttaa aaaaaaggct
     3481 aaagtaaaaa tcatatccca taattttact cgaaaacttt cggaaaagaa tccggatagt
     3541 aagctgatga aagcatatat ggatgcccat gtgtgttcaa atgtttatac tgtgcaggga
     3601 ggtaaggcgc attcccatta ttgtcatcgg gctttttgtc ctatttgtca gcgtattcaa
     3661 acggcacaaa atgtaaagaa gttccttccg gttatgaagt atttggcttc ggaaggcaga
     3721 gagttttatt ttgtgacgct taccttgcaa aattgtgtaa ccgatgatgc aagggtattg
     3781 cgagatttca tgcgtagatg taaccggatg tgggctgatg gtataaggac gaaacacaag
     3841 tttcgttcac ttggtatttc gggggtgatt aagaaggagt gtacctatca tgtagtaaaa
     3901 agggatttgt tttcgttcca ttatcatttt catattattg ttgattcatt ggaagctgca
     3961 aaatatgtag tagcccaatg gaagaaattg catggtagta cggttgctga tgcccgtttt
     4021 cagaaatata agaagataga ggattttgaa aatgcagcga tagaggtttt taagtatgct
     4081 tcaaaggctt ctgtctcaaa atcgaaaggt cgtgacggta aaaaaaagat tcagataaat
     4141 tataaggctt tggatatgat ttatactgcc atgcatggga tgcagcgcat gagtagtttc
     4201 ggacagttcc gcaccatgat cggagatgaa gcgttgaatg attttagaga tgaggatttg
     4261 gttcttaata ttgagggatt gaatgttgaa gatggttgtt atacttggta tatgtctgtg
     4321 aaagataagg tggctgattg gtataatatg gaaactgggg aggcattgtg ttgttataag
     4381 gtcacccgga aggatgaact tttgcaggat atttatttag atgaggatat ttttcctaat
     4441 agtgggtagt tgttagtttg ttgtattgtt ttcttggtat ggtgtgagtt ctatgtattc
     4501 tctattcatg aaaggatcgc caaattttcc acttctgtca tagaaggaaa atttgttttt
     4561 agttagcttg tctatgtccc agtaatgttc tccaccaaaa taaatggtac tgaaacttgg
     4621 atcaccgaat gac
//
