LOCUS       CP103246                4079 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-7 plasmid unnamed2, complete
            sequence.
ACCESSION   CP103246
VERSION     CP103246.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323522
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 254x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 22:01:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,304
            CDSs (total)                      :: 4,211
            Genes (coding)                    :: 4,143
            CDSs (with protein)               :: 4,143
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 39 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 9 of 68
            Pseudo Genes (multiple problems)  :: 11 of 68
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-7"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="1985-09-25"
     gene            1..252
                     /locus_tag="NXW83_21490"
     CDS             1..252
                     /locus_tag="NXW83_21490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR88947.1"
                     /translation="MEALSVREYRNNLAASFTKADNGEQVLIRRKNEIYALVKVGREDL
                     MITPELQARIDKAREEIKSGKCVTLKSSEDIDAYFDSL"
                     /name="hypothetical protein"
     gene            249..524
                     /locus_tag="NXW83_21495"
     CDS             249..524
                     /locus_tag="NXW83_21495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004519 - endonuclease activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family toxin"
                     /protein_id="UVR88948.1"
                     /translation="MMYTIRVSDGVDKVIAKWKKSNPNLFKKYKKIYKELLEHPKTGLG
                     HPEALRGGGDITWSRHITAHDRIIYDIYEEVVEVYILEVEGHYNDK"
                     /name="type II toxin-antitoxin system YoeB family toxin"
     gene            535..732
                     /locus_tag="NXW83_21500"
     CDS             535..732
                     /locus_tag="NXW83_21500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR88949.1"
                     /translation="MVEYCVYWLENGEPMHEVFSSLAAAEMYSCAIRGKENIEWVEVSE
                     EETIDLDELEDMFPDDFCGV"
                     /name="hypothetical protein"
     gene            1221..1946
                     /locus_tag="NXW83_21505"
     CDS             1221..1946
                     /locus_tag="NXW83_21505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UVR88950.1"
                     /translation="MIGKGKSISHGVAALEYDLAKEINGQAVATEIARHELYGCTGAEM
                     VQEMKPYHIDFPNVKNNCLRFEVSPSIEESATFTDADWAELGNDFMQRMGLANHQYIII
                     RHSGTESKKEQAHLHILANRVSLSGELYRDNWIGKKATEAANAIAKERNFVQSQDIGKV
                     NKAEIKEAMDGVLKKMQGFDFTKFKEELGKRGFKVREARASTGKLNGYYVTARSGTEYN
                     SMNISHGNKLTPGSGSFQR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1903..2355
                     /locus_tag="NXW83_21510"
     CDS             1903..2355
                     /locus_tag="NXW83_21510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR88951.1"
                     /translation="METNSHPEAAASNVKQGQYSPRKRKDTTPRVNPQLAVELVYQELK
                     RVEVYTKRIEDATARKVQIDGKSLESAENRLKNVLADFERQGYRMKNGGYVDKRISFYS
                     ILCAVISLLFACFMCYLWTDAAKDRDNYKQYYEYYQEQAREQKGNK"
                     /name="hypothetical protein"
     gene            2375..2605
                     /locus_tag="NXW83_21515"
     CDS             2375..2605
                     /locus_tag="NXW83_21515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="charged multivesicular body protein 2b-B"
                     /protein_id="UVR88945.1"
                     /translation="MGTTEHEEPRFFFILNKGAKSGGEITHAVLNGSIVSKPAGWDAFH
                     GLALAREKLSSEEIQQQMKELGVEMEIVPLI"
                     /name="charged multivesicular body protein 2b-B"
     gene            complement(2642..3667)
                     /locus_tag="NXW83_21520"
     CDS             complement(2642..3667)
                     /locus_tag="NXW83_21520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UVR88946.1"
                     /translation="MKKKLPITKNKDVVVSWVYTWSKQQDMSIHEQRIVLRILEACQAE
                     LKGVKLKDYAGTKRKFEHGLWDVDAQMHVSDVIFSGRDYNEIIAALDSLAGRFFTYEDD
                     EEWWKCGFISNPKYKKRTGIITFRVSNDLWDVFTKFAKGYREFELNKALALPTGYSLRF
                     YMLMSGQVYPLDISLENLKDRLGIPADKYKDKNGKDRIDHFEERVLKPAKAALDESCPY
                     TFNYVKVRENPNNKRSKVTGFRFYPVYQPQFRDEELEGKELQAKVTARYQIDSHVYEYL
                     RYSCGFTSEEINRNKETFITAQEKITDLIGELALLNGKSREKNNPKGWIINALKGKIKD
                     K"
                     /name="replication initiation protein"
     Typing          1222..1898
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="80.531"
                     /coverage="86.0"
                     /evalue="2.9400000000000003e-124"
ORIGIN
        1 atggaagcat tatcagtaag agaataccgt aacaacctcg cagcgtcttt caccaaagct
       61 gacaatggcg agcaggtact aattcgcagg aaaaacgaga tttacgcttt ggtaaaagtt
      121 ggtcgtgaag atttgatgat aaccccggag ctgcaagcaa ggattgacaa ggcaagggaa
      181 gaaatcaaat ccggaaagtg tgttaccctc aaaagcagtg aggatattga cgcttatttc
      241 gatagtctat gatgtacaca attagggttt ccgacggggt ggataaggtg attgccaaat
      301 ggaagaaatc aaaccccaat ttgttcaaga agtataagaa aatctacaaa gaattgttag
      361 agcaccctaa gactgggctt gggcatcccg aagcgttaag gggcggtggg gatattacat
      421 ggtctcgaca cattaccgcc catgaccgga taatctatga catttacgag gaagtggtag
      481 aggtctacat cttggaggta gaggggcatt acaacgataa atgataaaac tgttatggtc
      541 gaatattgtg tttactggtt agagaacggc gagcctatgc acgaggtgtt ctcttctctt
      601 gccgccgccg agatgtactc atgtgcgata agagggaaag aaaacattga atgggtggag
      661 gtgtccgaag aagaaaccat tgatttggac gaactggaag acatgttccc ggatgacttc
      721 tgcggcgtgt aatccccttg ggtagaaatg gggtagaatt tgttaggctg tgggggattt
      781 tctaacacgc cggggctttt tgctgggcgg ttggcgtgga tgtattccca tgcggcatgt
      841 gtatatatag caagaagtgt ccttgtcgga caattcttgc ttttctcgct ttgctcaaaa
      901 agattttaag attacctttg tggcatggaa ctaagacgga acgaaaagat tacattccgg
      961 tgtaccgaac ttgaaaagga cgcacttgcg gagcaggcag cccggtgcag tctaagcgta
     1021 tcggaatact gccggagttt gtcccttggg gggcgcccaa gggagaggta caccgaggag
     1081 gaacggcagc ttctccggga catagcccag ttgaaaggaa cgctccaacg gctgaacaac
     1141 tacttcggtg gtcgccagta ccgggaagtg ttcgaggaga accgggcact aattacagag
     1201 ctcaaaaaaa tactctcacg atgataggga aagggaaaag catatcacat ggtgtggcgg
     1261 cattggagta cgacctcgcc aaagagataa acgggcaggc agtagccacc gagatagccc
     1321 ggcatgaact ttatgggtgt actggtgcgg aaatggtgca ggaaatgaaa ccgtatcaca
     1381 ttgattttcc gaacgtaaaa aacaactgcc tacgtttcga ggtcagcccc tcgatagagg
     1441 aaagcgccac ctttacggat gcggactggg cggaacttgg taacgacttc atgcagcgca
     1501 tggggttggc gaaccaccag tacatcatca tccggcacag cggtacggag agcaagaaag
     1561 agcaagccca cctgcacata ctggcaaacc gggtttccct ttccggggaa ctgtaccggg
     1621 acaactggat agggaaaaag gcaacggaag ccgccaatgc catagccaag gaacggaact
     1681 ttgtacagtc gcaggacatt ggcaaagtca acaaagcgga aatcaaggaa gccatggacg
     1741 gcgtattgaa gaagatgcag ggctttgact ttaccaaatt caaggaagaa cttggtaaga
     1801 gaggttttaa agtccgggaa gccagggcaa gcaccgggaa acttaacggc tactatgtca
     1861 cagcccgaag cggtacggag tataactcaa tgaacatatc tcatggaaac aaactcacac
     1921 ccggaagcgg cagcttccaa cgttaagcag ggacagtaca gcccccggaa aagaaaagat
     1981 acaacgccaa gggtaaaccc gcagcttgcg gtagaactgg tgtatcaaga actaaagcgt
     2041 gttgaagtct atacgaagcg catagaggac gcaacagccc gaaaggtaca aatagacggg
     2101 aaaagccttg aaagtgccga aaatcgccta aaaaacgtgt tggcggactt tgaacggcag
     2161 gggtacagaa tgaaaaacgg cggttatgtg gacaaacgaa tttcgttcta ctcaatcctt
     2221 tgtgctgtta tctccctatt gttcgcttgt ttcatgtgct atctttggac ggatgcagcc
     2281 aaagaccggg acaactacaa gcagtattat gaatactacc aagagcaggc aagggaacaa
     2341 aaggggaata aataaacatc taactttacg aattatggga acaacggaac acgaagaacc
     2401 ccggttcttc ttcatactga acaagggtgc gaagtccggc ggcgaaatca cccatgccgt
     2461 cctaaacggt agtatcgttt cgaagccggc aggctgggac gcctttcatg ggcttgcact
     2521 ggcgagagag aaactaagca gcgaggaaat acaacagcag atgaaagaac ttggtgtaga
     2581 aatggaaatc gttcctctga tttgacaaga ttacccggag tgtttcagcc gggtaatctt
     2641 gttacttatc cttgattttc cctttaaggg cgtttataat ccaccctttc ggattgttct
     2701 tttctcgtga ttttccgttt aggagagcca gttctccgat aaggtcggtt atcttttctt
     2761 gtgccgttat gaatgtctct ttgttccggt ttatctcttc cgatgtgaag ccgcaggaat
     2821 aacggaggta ctcgtacaca tggctgtcta tctgatatcg tgctgtaacc tttgcttgca
     2881 attctttccc ttccagttct tcatctctga actgtggttg atagaccggg tagaacctaa
     2941 acccggtcac cttgctacgt ttgttgtttg ggttttcacg cactttcacg tagttgaaag
     3001 tgtatgggca gctctcatca agcgcagcct ttgctggttt caaaactctt tcctcaaaat
     3061 gatctattct gtcttttccg ttcttgtcct tgtatttgtc cgcaggtatg ccaagacggt
     3121 ctttcaagtt ttcaagggat atatccaaag ggtacacctg cccactcatt aacatgtaaa
     3181 acctaaggga atacccagta ggtagcgcaa gagccttgtt taactcaaat tctctgtacc
     3241 ccttggcgaa cttggtaaac acatcccaaa ggtcgttaga cacacgaaag gttataatgc
     3301 cagtgcgttt tttatatttt gggttagata taaatccgca cttccaccat tcctcgtcat
     3361 cttcgtaagt aaaaaaacgt cctgccaaag aatcgagtgc ggctatgatt tcattgtaat
     3421 cacgtccgga aaaaataacg tcagatacat gcatttgtgc atcaacatcc caaagtccat
     3481 gctcaaactt gcgtttcgtg cctgcataat ctttcagttt tactcccttt agttcagctt
     3541 ggcacgcttc cagtatgcga agaactattc tttgttcgtg tatggacatg tcttgctgtt
     3601 ttgaccatgt atatacccat gaaacaacaa catctttatt cttggtaata ggtagtttct
     3661 ttttcataaa cgattgattt gctgcaaata tacacaatcc ccacattaaa acaaaaaaag
     3721 aggggtaaaa ctcccaaagt gtggggacta atttactttt aaactcccaa agtgtgggga
     3781 ctaaactccc aaagtgtggg gaaacacatg ttctaatccc tttatttatg ggcgtttgcg
     3841 aagaagtgat agagcatatt acagacgatg cttataacag acaaggggaa tttatagagc
     3901 taagtggttg atttactgtc ggaaaaagaa aaacgatagg gaaaaagagc aaagcaaaac
     3961 caaccaacgg gcggtaggat tggaaacggg tttttatggc gtgtacacgt ttgtgtacaa
     4021 aatgtttgta ttttattgaa atatagctat ctttgtgctg aatttaaaag caaacagat
//
