LOCUS       CP103237                4069 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-79 plasmid unnamed2, complete
            sequence.
ACCESSION   CP103237
VERSION     CP103237.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323521
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 190x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 21:53:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,576
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,333
            CDSs (with protein)               :: 4,333
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 113 of 148
            Pseudo Genes (incomplete)         :: 34 of 148
            Pseudo Genes (internal stop)      :: 21 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-79"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="1985-10-04"
     gene            1..1026
                     /locus_tag="NXX67_22850"
     CDS             1..1026
                     /locus_tag="NXX67_22850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UVS06464.1"
                     /translation="MKKKLPITKNKDVVVSWVYTWSKQQDMSIHEQRIVLRILEACQAE
                     LKGVKLKDYAGTKRKFEHGLWDVDAQMHVSDVIFSGRDYNEIIAALDSLAGRFFTYEDD
                     EEWWKCGFISNPKYKKHTGIITFRVSNDLWDVFTKFAKGYREFELNKALALPTGYSLRF
                     YMLMSGQVYPLDISLDNLKDRLGIPADKYKDKNGKDRIDNFEERVLKPAKAALDESCPY
                     TFNYVKVRENPNNKRSKVTGFRFYPVYQPQFRDEELEGKELQAKVTARYQIDSHVYEYL
                     RYSCGFTSEEINRNKETFITAQEKITDLIGELALLNGKSREKNNPKGWIINALKGKIKD
                     K"
                     /name="replication initiation protein"
     gene            complement(1063..1293)
                     /locus_tag="NXX67_22855"
     CDS             complement(1063..1293)
                     /locus_tag="NXX67_22855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="charged multivesicular body protein 2b-B"
                     /protein_id="UVS06465.1"
                     /translation="MGTTEHEEPRFFFILNKGAKSGGEITHAVLNGSIVSKPAGWDAFH
                     GLALAKEKLSSEEIQQQMKELGVEMEIVPLI"
                     /name="charged multivesicular body protein 2b-B"
     gene            complement(1312..1764)
                     /locus_tag="NXX67_22860"
     CDS             complement(1312..1764)
                     /locus_tag="NXX67_22860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS06466.1"
                     /translation="METNSHPEAAASTVKQGQYSPRKRKDTAPKVNPQLAIELFFQEMG
                     RIEAYTKRIEDATSRKVQIDGKSLESAENRLKNVLSDFERQGYRMKNGGYVDKRISFYS
                     ILCAVISLLFACFMCYLWTDAAKDRDNYKQYYEYFQEQAREQKGNK"
                     /name="hypothetical protein"
     gene            complement(1721..2515)
                     /locus_tag="NXX67_22865"
     CDS             complement(1721..2515)
                     /locus_tag="NXX67_22865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UVS06467.1"
                     /translation="MIGKGKSISHGTAALEYDLAKEIDGQTAAIEIARHELYGCTGAEM
                     VQEMKPYHADFPNVKNNCLRFEVSPSIEESATFTDADWAELGNDFMQRMGLANHQYIII
                     RHSGTESKKEQAHLHILANRVSLSGELYRDNWIGKKATEAANAIAKERNFVQSKDIGKA
                     NKAEIKEAMDDVLKKMQGFDLTKFKEELGRRGFKVREARASTGKLNGYYVTARSGTEYK
                     ASEIGKGYTLAHIERTQSKLKYNSMNISHGNKLTPGSGSFHR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2926..3123)
                     /locus_tag="NXX67_22870"
     CDS             complement(2926..3123)
                     /locus_tag="NXX67_22870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS06461.1"
                     /translation="MVEYCVYWLENGEPMHEVFSNLAAAEMYSCAIRGKENVEWVEVSE
                     EEAIDLDELEDMFPDDFCGV"
                     /name="hypothetical protein"
     gene            complement(3134..3409)
                     /locus_tag="NXX67_22875"
     CDS             complement(3134..3409)
                     /locus_tag="NXX67_22875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004519 - endonuclease activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family toxin"
                     /protein_id="UVS06462.1"
                     /translation="MMYTIRVSDGVDKVIAKWKKSNPNLFKKYKKIYKELLEHPKTGLG
                     HPEALRGGGDITWSRHITAHDRIIYDIYEEVVEVYILEVEGHYNDK"
                     /name="type II toxin-antitoxin system YoeB family toxin"
     gene            complement(3406..3657)
                     /locus_tag="NXX67_22880"
     CDS             complement(3406..3657)
                     /locus_tag="NXX67_22880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS06463.1"
                     /translation="MEALSVREYRNNLAASFTKADNGEQVLIRRKNEIYALVKVGREDL
                     MITPELQARIDKAREEIKSGKCVTLKSSEDIDAYFDSL"
                     /name="hypothetical protein"
ORIGIN
        1 atgaaaaaga aactacctat taccaaaaac aaagatgttg ttgtttcatg ggtatataca
       61 tggtctaaac agcaagatat gtccatacac gaacaaagaa tagtacttcg tatcttggaa
      121 gcatgccagg cagaattgaa aggggtaaag ctgaaagatt atgcaggtac gaaacgcaaa
      181 tttgagcatg gactttggga tgttgatgca caaatgcatg tctcggacgt tattttttcc
      241 ggacgtgatt acaatgaaat catagccgca ctcgattctt tggcaggacg ttttttcact
      301 tacgaagatg acgaggagtg gtggaagtgc ggatttatat ctaatcccaa atataaaaaa
      361 cacactggca ttataacctt tcgtgtgtct aatgaccttt gggatgtgtt taccaagttc
      421 gccaaggggt acagagagtt tgaattaaac aaggctcttg cgctgcctac cgggtattcc
      481 cttaggtttt acatgctaat gagtgggcaa gtgtaccctt tggatatatc ccttgacaac
      541 ttgaaagacc gccttggcat accagcggac aagtacaagg acaagaacgg aaaagataga
      601 attgacaact tcgaggaaag ggttttaaaa cctgcaaagg ctgcgcttga tgagagttgc
      661 ccatacactt tcaactacgt gaaagtgcgt gaaaacccaa acaacaaacg tagcaaggtg
      721 accggattta ggttttaccc ggtctatcag ccacagttca gggatgaaga actggaaggg
      781 aaagaattgc aggcaaaggt tacagcacga tatcagatag acagccatgt atacgagtat
      841 ctccgttatt cctgcggttt cacatcggaa gagataaacc ggaacaaaga gacattcata
      901 acggcacaag aaaagataac cgaccttatc ggagaactgg ctctcctaaa cggaaaatca
      961 cgagaaaaga acaatccgaa agggtggatt ataaacgccc ttaaagggaa aatcaaggat
     1021 aagtaacagg attacccggc tgaaacactc cgggtaatct tgtcaaatca gaggaacgat
     1081 ttccatttct acaccaagtt ctttcatctg ctgttgtatt tcctcgctgc ttagtttctc
     1141 tttcgccagt gcaagcccat gaaaggcgtc ccagcctgcc ggcttcgaaa cgatactacc
     1201 gtttaggacg gcatgggtga tttcgccgcc ggacttcgca cccttgttca gtatgaagaa
     1261 gaaccggggt tcttcgtgtt ccgttgttcc cataattcgt aaagttagag tttatttgtt
     1321 tcccttttgt tcccttgcct gctcttggaa gtattcataa tactgcttgt agttgtcccg
     1381 gtctttggct gcatccgtcc aaagatagca catgaaacaa gcgaacaata gggagataac
     1441 agcacaaagg attgagtaga acgaaatccg tttgtccaca taaccgccgt ttttcattct
     1501 gtacccctgc cgttcaaagt ccgacaatac gttttttagg cgattttcgg cactttcgag
     1561 gcttttcccg tctatttgta cctttcggga tgttgcatcc tctatgcgct ttgtataggc
     1621 ttcaatacgt cccatttctt gaaaaaacag ttctatcgca agctgcgggt taacctttgg
     1681 tgctgtgtct tttcttttcc gggggctgta ctgtccctgc ttaacggtgg aagctgccgc
     1741 ttccgggtgt gagtttgttt ccatgagata tgttcattga gttatatttc agtttgcttt
     1801 gtgtccgctc gatatgggcg agagtgtagc ctttccctat ctcgctcgcc ttatactccg
     1861 taccgcttcg ggctgtgacg tagtagccgt taagttttcc ggtgcttgcc ctggcttccc
     1921 gaactttaaa acctctccta ccaagttctt ccttgaattt agtcaagtca aagccctgca
     1981 tcttcttcaa gacgtcgtcc atggcttcct tgatttccgc tttgttggct ttgccgatgt
     2041 ccttcgactg tacaaagttc cgttccttgg ctatggcgtt ggcggcttcc gttgcctttt
     2101 tccctatcca gttatcccgg tacagttccc cggaaaggga aacccggttt gccagtatat
     2161 gcaggtgggc ttgttctttt ttgctctccg taccgctgtg ccggatgatg atgtactggt
     2221 ggttcgccag ccccatgcgc tgcatgaagt cgttaccaag ttccgcccag tccgcatccg
     2281 taaaggtggc gctttcctct atcgaaggac tgacctcgaa acgtaggcaa ttgtttttta
     2341 cgttcggaaa atcagcgtgg tatggtttca tttcctgtac catttccgca cccgtacacc
     2401 cgtaaagttc atgccgggct atctcgatgg ctgctgtctg tccgtctatc tctttggcaa
     2461 ggtcatactc cagtgcagcc gtaccatgcg atatgctctt tcctttccct atcatcgtga
     2521 aagtattttt ttaagttcgg tgattagtgc ctgattctct tcaaatacct cccggtactg
     2581 gcgaccaccg aagtagttgt tcagccgttg gagcgttcct ttcaactggg ctatgtcccg
     2641 gagaagctgc cgttcctcgc ctgctccgca agtgccagct tttcataccg ggtacagcgg
     2701 aatgtaatct tttcgttccg tcttacttcc atgtcacaaa ggtaatctta aaatcttttt
     2761 gagcaaagcg agaaaagcaa gaattgtccg acaaggacac ttcttgctat atatacacat
     2821 gccgcatggg aatacatcca tgctaaccgc ccggcaaaaa gccccggcgt gttagaaaat
     2881 ccccccacag cctaacaatt tctaccccat ttctacccaa ggggattaca cgccgcagaa
     2941 gtcatccggg aacatgtctt ccagttcgtc caaatcaatg gcttcttctt cggacacctc
     3001 cacccattca acgttttctt tccctcttat cgcacatgag tacatctcgg cggcggcaag
     3061 gttagagaac acctcgtgca taggctcgcc gttctctaac cagtaaacac aatattcgac
     3121 cataacagtt ttatcattta tcgttgtaat gcccctctac ctccaaaata tagacctcta
     3181 ccacttcctc gtaaatgtca tagattatcc ggtcatgggc ggtgatgtgt cgagaccatg
     3241 taatatcccc accgcccctt aacgcttcgg gatgcccaag cccagtctta gggtgctcta
     3301 acaattcttt gtagattttc ttatacttct tgaacaaatt ggggtttgat ttcttccatt
     3361 tggcaatcac cttatccacc ccgtcggaaa ccctaattgt gtacatcata gactgtcgaa
     3421 ataagcgtca atatcctcac tgcttttgag ggtaacacac tttccggatt tgatttcttc
     3481 ccttgccttg tcaatccttg cttgcagctc cggggttatc atcaaatctt cacgaccaac
     3541 ttttaccaaa gcgtaaatct cgtttttcct gcgaattagt acctgctcgc cattgtcagc
     3601 tttggtgaaa gacgctgcga ggttgtttcg gtattctctt actgataatg cttccatatc
     3661 tgtttgcttt taaattcagc acaaagatag ctatatttca ataaaataca aactttttgt
     3721 acacaaatgt gtacacgaca taaaaagccg ctcacagtcc taccgcccgt tggttggttt
     3781 tgctttgctc tttttcccta ttgtttttct ttttccgaca gtaaatcagc catttaactc
     3841 tataaatttc ccttgtctgt tataagcatc gtctgtaata tgctctatca cttcttcgca
     3901 aatacccatg aataaaggga ttaaagcatg agtttcccta cattttggga gttttaatcc
     3961 tacactttgg gagtttttca cctcactatt ccctacactt tgggagtttt acccttcttt
     4021 ttttgtttca atgtaggttt tatgtatatt tgcagcaaat caatcgctt
//
