LOCUS       CP103236                5582 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-79 plasmid unnamed1, complete
            sequence.
ACCESSION   CP103236
VERSION     CP103236.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323521
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 190x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 21:53:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,576
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,333
            CDSs (with protein)               :: 4,333
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 113 of 148
            Pseudo Genes (incomplete)         :: 34 of 148
            Pseudo Genes (internal stop)      :: 21 of 148
            Pseudo Genes (multiple problems)  :: 16 of 148
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-79"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="1985-10-04"
     gene            1..1251
                     /locus_tag="NXX67_22810"
     CDS             1..1251
                     /locus_tag="NXX67_22810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS06457.1"
                     /translation="MAKTCIRVEACNIGSSERHNLRSKELDYIRPELTHRNEQWVECSI
                     AEVHRDITEKYKEATGQGLQKKATPIREGVIVISEETTIQQLQDLAEKLEERFGVHAFQ
                     IYTHKDEGANVWDGKEEAWKPNYHAHMIFDWTDGHTGKTVKLNRHDMAEMQTITAECLN
                     MERGVSSDKKHLSAMQYKNKMETEKAEQLQKDIEQLNRAYTAGTEKITTVQKELSTAQK
                     ELNSMKTDIHINEAKEAAAKAGKTVFNAVTSVFNVGEVKRQAKEIETLKKENYNLSFKN
                     QNLEGQLRTNNIEMRRVQETTDRQIKAEASKLKPITNLFPEMENAQENIEELQTMGIQN
                     KDIRQLLIGKEIYYTGNLYDKDKRKSYQVKNVKIDISKSQNGITTIWLNNIHFKNFLKE
                     LWNTLQKTLDNGNWLHR"
                     /name="hypothetical protein"
     gene            complement(1321..2091)
                     /locus_tag="NXX67_22815"
     CDS             complement(1321..2091)
                     /locus_tag="NXX67_22815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS06458.1"
                     /translation="MKKVMFMFISLLTVLGVCSCNDNEVIEPVVSDSVSDMEVLSRFVD
                     VNEITNEYYFNENKKTRALSYVTGSDWQDLEKVSPLSIEKYKNNLQVLNAQVASAISNP
                     NTAYVVFSVNGKTLVKKVKEDANFDFSVFRDVVTKTRAVLPSLSINGGSQSTTGVFYDS
                     SRTLKMQVDLNASIQNNYYFFEVLNPNAKPSPDDNITTPESVAFSGTGPLWSNTFTWTS
                     YWDANVPGQGFKWEFKGKGTTPSFGFIANCTFSR"
                     /name="hypothetical protein"
     gene            2183..2644
                     /locus_tag="NXX67_22820"
     CDS             2183..2644
                     /locus_tag="NXX67_22820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS06459.1"
                     /translation="MNRIIHISLLISTVSLFLISNTGCVEKQGYYNHGEESIISLICDI
                     TWAGKKTTDENGSVWQGTYKFNKNGTYTRTNIEIDKQGNKKEANIYGQWSFGDPSFSTI
                     YFGGEHYWDIDELTKNKFSFYDRSGKFGDPFMNREYIELTPYQENNTTN"
                     /name="hypothetical protein"
     gene            complement(2648..3106)
                     /locus_tag="NXX67_22825"
     CDS             complement(2648..3106)
                     /locus_tag="NXX67_22825"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS06460.1"
                     /translation="MEEIAWYARFQKYKKIEDFENAAIEVFKYASKASVSKSKGRDGKK
                     KIQINYKALDMIYTAMHGMQRMSSFGQFRTMIGDEALNDFRDEDLVLNIEGLNVEDGCY
                     TWYMSVKDKVADWYNMETGEALCCYKVTRKDELLQDIYLDEDIFPNSG"
                     /name="hypothetical protein"
     gene            complement(3058..3537)
                     /locus_tag="NXX67_22830"
     CDS             complement(3058..3537)
                     /locus_tag="NXX67_22830"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="UVS06453.1"
                     /translation="MKAYMDAHVCSNVYTVQGGKAHSHYCHRAFCPICQRIQTAQNVKK
                     FLPVMKYLASEGREFYFVTLTLQNCVTDDARVLRDFMRRCNRMWADGIRTKHKFRSLGI
                     SGVIKKECTYHVVKKDLFSFHYHFHIIVDSLEAAKYVVAQWKKLHGMPVFRNIRR"
                     /name="protein rep"
     gene            complement(4180..4578)
                     /locus_tag="NXX67_22835"
     CDS             complement(4180..4578)
                     /locus_tag="NXX67_22835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-poly-alpha-D-galacturonosidase"
                     /protein_id="UVS06454.1"
                     /translation="MKPTDYIEWDNLKDIPFFLCQVVEDREKQDLDIYYLGKRVLHDYD
                     HVGHYLRTAVILFRRVKSRTADWVNLRNLWTLRNCVRENYNHGIGMNDLIFGENFDGDN
                     LDTLTPLTKKRFDFLCKRIKELDPYATI"
                     /name="exo-poly-alpha-D-galacturonosidase"
     gene            complement(4615..4935)
                     /locus_tag="NXX67_22840"
     CDS             complement(4615..4935)
                     /locus_tag="NXX67_22840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="UVS06455.1"
                     /translation="MVEQYEVYWVELDPTRGGEMAKTRPCVVVTPSDLNMYLTTVVIVP
                     ITSTIRNYPYRVLCSVAGREGEIATDQIRTVDKSRLKRKIGDLNFSEIKRLREVFQQMF
                     CE"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(4929..5177)
                     /locus_tag="NXX67_22845"
     CDS             complement(4929..5177)
                     /locus_tag="NXX67_22845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="UVS06456.1"
                     /translation="MVTNIIQIGNSKGIILPSEVLKQLRLSLKSAVSISLDGNNIVIKA
                     QPRQGWAEAAKRAHENGDDELLIPDVFEDEKFEDWTW"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     Typing          complement(2649..3684)
                     /name="rep_cluster_870"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.571"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..1248
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaaga catgtatcag agtggaagcc tgcaatatag gcagctcgga acgtcacaac
       61 ttaagaagca aggagctcga ctacatccgt ccggaactca cacaccgcaa cgaacagtgg
      121 gtagaatgtt ccattgccga agtacatcgg gacataacgg aaaaatacaa ggaagccacc
      181 ggacaaggat tacagaagaa agctacccca atccgggaag gggtaatagt aatcagcgag
      241 gaaaccacaa tccagcagct tcaagacttg gccgaaaaac tggaagaacg ctttggagtg
      301 cacgccttcc aaatctacac ccataaggat gaaggcgcaa acgtatggga cggaaaggaa
      361 gaagcatgga aaccgaacta tcatgcgcac atgatctttg actggacgga cggccataca
      421 ggaaaaactg taaagctgaa cagacacgac atggcggaga tgcagactat aacagcagag
      481 tgtctcaaca tggaacgtgg agtttcttca gacaagaaac acttgtcggc catgcaatac
      541 aaaaacaaaa tggaaacaga gaaagctgaa caacttcaaa aagacatcga gcaactaaac
      601 agggcataca ctgccggaac cgagaaaatc accacagtcc aaaaggaact tagtacggca
      661 caaaaagagc taaacagcat gaaaaccgac atacacatca acgaagccaa agaagctgcc
      721 gcaaaagccg gaaaaaccgt atttaatgcc gttacaagcg tctttaatgt aggggaagta
      781 aaacgacaag ccaaagaaat tgaaaccctc aaaaaagaaa attataacct ctcattcaag
      841 aatcaaaatt tagaaggaca attaagaacc aacaacatcg agatgcgaag ggtacaggaa
      901 acaaccgaca gacagataaa ggcagaagca tccaaactaa aacccataac caaccttttt
      961 ccggaaatgg aaaatgcaca ggaaaatata gaggaactcc aaactatggg aatacaaaat
     1021 aaagatatcc gacaacttct tattggaaag gaaatatact acactggaaa cctttacgat
     1081 aaagacaaga gaaaatcata ccaagttaaa aacgtgaaaa tagatatctc caaaagtcag
     1141 aatggtatta ctacaatttg gctaaataac atccacttta aaaactttct taaagaactt
     1201 tggaatacac tacaaaagac tttagataac ggtaactggc tacaccgata gaaagacact
     1261 aaaaagaatt gcacttctct tttttgagaa gtgcaattca aattaaatcc cgttcatttt
     1321 ttaacgacta aaggtgcaat tagctataaa accaaaagaa ggagtagtac cttttccttt
     1381 aaactcccat ttaaaacctt gaccaggtac atttgcatcc caatacgaag tccaagtgaa
     1441 agtgttactc cataacggtc cagttccact aaaagcaaca ctttcaggtg tcgttatatt
     1501 atcatccgga cttggtttag cattaggatt taatacttca aagaaataat aattattctg
     1561 tattgaagca ttcaaatcaa cttgcatctt taaagtacga ctggaatcgt aaaaaactcc
     1621 ggtagtgctt tgagaacctc cattaatact caaagaaggg agaacagccc tcgttttcgt
     1681 tacaacatcc ctaaatactg aaaaatcaaa attagcatcc tccttcacct ttttaaccaa
     1741 agtctttcca tttacagaaa aaacaacata agcggtatta ggatttgaaa tagcacttgc
     1801 cacttgagca tttaaaacct gcaggttatt cttatatttc tcaatactca aaggactaac
     1861 tttttctaaa tcctgccaat ccgaacctgt tacataagac aacgccctcg ttttcttatt
     1921 ctcattgaaa tagtattcat ttgtaatctc atttacatca acaaaacgag ataagacctc
     1981 catatcagat acagaatcac taaccacagg ttctattact tcattatcat tacacgaaca
     2041 aacacccaaa acagtcagta gactgatgaa cataaacatt acttttttca taaatcctcc
     2101 ttttttaaag ttaataaata aaggtttcgc ccaaaaatat aaaatttctc ctatttttgc
     2161 attgaatatt aaatatttaa ctatgaatag aataatacat atctctttac ttatttcaac
     2221 agtatcactt tttcttattt caaacaccgg atgtgttgaa aaacaaggat actacaacca
     2281 tggagaagaa agcataatct cattaatttg tgatattaca tgggctggta aaaaaacaac
     2341 agacgaaaac ggcagtgtct ggcaaggtac atacaaattc aataaaaacg gaacatatac
     2401 ccgtacaaat atagaaatcg acaaacaagg aaataaaaaa gaagccaata tctacggaca
     2461 atggtcattc ggtgatccaa gtttcagtac catttatttt ggtggagaac attactggga
     2521 catagacgag ctaactaaaa acaaattttc cttctatgac agaagtggaa aatttggcga
     2581 tcctttcatg aatagagaat acatagaact cacaccatac caagaaaaca atacaacaaa
     2641 ctaacaacta cccactatta ggaaaaatat cctcatctaa ataaatatcc tgcaaaagtt
     2701 catccttccg ggtgacctta taacaacaca atgcctcccc agtttccata ttataccaat
     2761 cagccacctt atctttcaca gacatatacc aagtataaca accatcttca acattcaatc
     2821 cctcaatatt aagaaccaaa tcctcatctc taaaatcatt caacgcttca tctccgatca
     2881 tggtgcggaa ctgtccgaaa ctactcatgc gctgcatccc atgcatggca gtataaatca
     2941 tatccaaagc cttataattt atctgaatct tttttttacc gtcacgacct ttcgattttg
     3001 agacagaagc ctttgaagca tacttaaaaa cctctatcgc tgcattttca aaatcctcta
     3061 tcttcttata tttctgaaaa cgggcatacc atgcaatttc ttccattggg ctactacata
     3121 ttttgcagct tccaatgaat caacaataat atgaaaatga taatggaacg aaaacaaatc
     3181 cttttttact acatgatagg tacactcctt cttaatcacc cccgaaatac caagtgaacg
     3241 aaacttgtgt ttcgtcctta taccatcagc ccacatccgg ttacatctac gcatgaaatc
     3301 tcgcaatacc cttgcatcat cggttacaca attttgcaag gtaagcgtca caaaataaaa
     3361 ctctctgcct tccgaagcca aatacttcat aaccggaagg aacttcttta cattttgtgc
     3421 cgtttgaata cgctgacaga taggacaaaa agcccgatga caataatggg aatgcgcctt
     3481 acctccctgc acagtataaa catttgaaca cacatgggca tccatatatg ctttcatcag
     3541 cttactatcc ggattctttt ccgaaagttt tcgagtaaaa ttatgggata tgatttttac
     3601 tttagccttt tttttaagac tatcagaacc gttttttaac ggactttctt tgcagttggt
     3661 tatatttgtt ctatctttgc acatatgaaa ttttgatgtg gctttcgttt ggtatccgcc
     3721 ccgccaagaa cgaacacaaa cgattgctaa ttcttaaaat acaaagcccg ttggttactg
     3781 ccttcgggct ttcgtttttt ctcagcccaa aagtaggtaa ttcattccaa accgccaaac
     3841 atcattcaaa aaacctacat ataacaagtc aaaaagcatt taccacgaaa ctttctagtt
     3901 tatctagtat gggggctcgc ccccattccc ccggctgcgc aattctgaca caaatacact
     3961 actaaatcca caacagacaa agatacttcc ggaaaaaaaa ctatctttgc cgtacacaaa
     4021 gacaaccctt gccgagcgat acgggagagt tgcaaaaaaa acaccggagt gaattgacgg
     4081 atatccgaca tctccggtgg tatttttttt tcttatcccc ctcccgtccc ccaaaggggg
     4141 agcacacaaa gacaagccct gccttcactc aatggagtgt taaatagttg catatgggtc
     4201 aagttcctta atccgcttac acaaaaaatc aaagcgtttc tttgtaagtg gtgtaagcgt
     4261 atcgagatta tctccatcga aattctcccc aaaaataagg tcgttcattc ctatgccgtg
     4321 attgtagttc tcacgcacac agttgcgcag cgtccaaagg ttacgcaggt ttacccagtc
     4381 tgctgtgcgg ctctttaccc tgcggaacaa gataacggca gtccgcaaat agtgccccac
     4441 atggtcgtaa tcgtgcaaga cacgtttgcc caaatagtaa atatccaaat cttgtttttc
     4501 tctgtcctcg actacttgac agaggaagaa tggaatatcc ttcaggttat cccattcgat
     4561 gtagtcagtt ggtttcataa tgtcttattt gcatttttct ttccgtactt tccattattc
     4621 acagaacatt tgctgaaata cttcacgtaa cctttttatc tcactgaaat tcaaatctcc
     4681 aatctttctt ttcagccggc ttttatccac cgtgcgaatt tgatcagtgg ctatctctcc
     4741 ttcacgcccg gctacagaac acagaacccg gtaaggataa tttcttatcg ttgaggttat
     4801 cggaacaatg actactgttg taaggtacat attcaaatct gaaggagtga caactacaca
     4861 tggccgtgtc tttgccattt cacccccacg ggtagggtct aactctaccc aatacacttc
     4921 atactgttct accatgtcca atcctcaaac ttttcatcct caaacacatc agggataagc
     4981 aactcatcat ccccattctc atgggcacgt ttggcagctt ccgcccatcc ctgccgtggt
     5041 tgtgccttta tcacaatatt gttcccgtcc aaagaaatgc tcacagccga tttcaaggac
     5101 aaacgcaact gtttcaatac ttcggaagga agtattatcc ccttgctgtt tccaatctga
     5161 ataatattcg ttaccataat gttataactt tataattgat tatcacgata caaatatata
     5221 taaagttata acaataacaa aaggaaaaac aacttttttc cattttcacc gagaaccctg
     5281 tcaatggctg cactcgtcgg gagtgttggt gcaccccctt gccgtgcagc gctccccgac
     5341 cgatgatttt ttattgtgcc ttgtgtcctt ttcccttttg tccggcttct gttcccaagc
     5401 acatccggaa cacatggcag agcgcttgca aatcccgtcc ggaaggctta cctttgcaaa
     5461 aggttatagc acactataac ccctaaaggg gttgtgtgcg aggggtgtcc cctcgacccc
     5521 gtgcgatgtg acgtttcacg ccacatcttc taaacagaca tacacaccta aaacctaaag
     5581 at
//
