LOCUS       CP103234                5585 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-300 plasmid unnamed1, complete
            sequence.
ACCESSION   CP103234
VERSION     CP103234.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323270
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 177x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 21:52:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,418
            CDSs (total)                      :: 4,323
            Genes (coding)                    :: 4,029
            CDSs (with protein)               :: 4,029
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 294
            CDSs (without protein)            :: 294
            Pseudo Genes (ambiguous residues) :: 0 of 294
            Pseudo Genes (frameshifted)       :: 243 of 294
            Pseudo Genes (incomplete)         :: 50 of 294
            Pseudo Genes (internal stop)      :: 28 of 294
            Pseudo Genes (multiple problems)  :: 20 of 294
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-300"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="1992-10-20"
     gene            148..738
                     /locus_tag="NXW55_22050"
     CDS             148..738
                     /locus_tag="NXW55_22050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="UVS02128.1"
                     /translation="MKAYMDAHVCSNVYTVQGGKAHSHYCHRAFCPICQRIQTAQNVKK
                     FLPVMKYLASEGREFYFVTLTLQNCVTDDARVLRDFMRRCNRMWADGIRTKHKFRSLGI
                     SGVIKKECTYHVVKKDLFSFHYHFHIIVDSLEAAKYVVAQWKKLHGSTVADARFQKYKK
                     IEDFENAAIEVFKYASKASVSKSKGRDGKKRFR"
                     /name="protein rep"
     gene            756..1049
                     /locus_tag="NXW55_22055"
     CDS             756..1049
                     /locus_tag="NXW55_22055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS02129.1"
                     /translation="MIYTAMHGMQRMSSFGQFRTMIGDEALNDFRDEDLVLNIEGLNVE
                     DGCYTWYMSVKDKVADWYNMETGEALCCYKVTRKDELLQDIYLDEDIFPNSG"
                     /name="hypothetical protein"
     gene            complement(1053..1514)
                     /locus_tag="NXW55_22060"
     CDS             complement(1053..1514)
                     /locus_tag="NXW55_22060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS02133.1"
                     /translation="MNRIIHISLLISTVSLFLISNTGCVEKQGYYNHGEESIISLICDI
                     TWAGKKTTDENGSVWQGTYKFNKNGTYTRTNIEIDKQGNKKEANIYGQWSFGDPSFSTI
                     YFGGEHYWDIDELTKNKFSFYDRSGKFGDPFMNREYIELTPYQENNTTN"
                     /name="hypothetical protein"
     gene            1606..2376
                     /locus_tag="NXW55_22065"
     CDS             1606..2376
                     /locus_tag="NXW55_22065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS02130.1"
                     /translation="MKKVMFMFISLLTVLGVCSCNDNEVIEPVVSDSVSDMEVLSRFVD
                     VNEITNEYYFNENKKTRALSYVTGSDWQDLEKVSPLSIEKYKNNLQVLNAQVASAISNP
                     NTAYVVFSVNGKTLVKKVKEDANFDFSVFRDVVTETRAVLPSLSINGGSQSTTGVFYDS
                     SRTLKMQVDLNASIQNNYYFFEVLNPNAKPSPDDNITTPESVAFSGTGPLWSNTFTWTS
                     YWDANVPGQGFKWEFKGKGTTPSFGFIANCTFSR"
                     /name="hypothetical protein"
     gene            complement(2446..2730)
                     /locus_tag="NXW55_22070"
     CDS             complement(2446..2730)
                     /locus_tag="NXW55_22070"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVS02131.1"
                     /translation="MENAQENIEELQTMGIQNKDIRQLLIGKEIYYTGNLYDKDKRKSY
                     QVKNVKIDISKSQNGITTIWLNNIHFKNFLKELWNTLQKTLDNGNWLHR"
                     /name="hypothetical protein"
     gene            complement(2748..3689)
                     /locus_tag="NXW55_22075"
     CDS             complement(2748..3689)
                     /locus_tag="NXW55_22075"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="UVS02132.1"
                     /translation="MAKTCIRVEACNIGSSERHNLRSKELDYIRPELTHRNEQWVECSI
                     AEVHRDITKKYKEATGQGLQKKATPIREGVIVISEETTIQQLQDLAEKLEERFGVHAFQ
                     IYTHKDEGANVWDGKEEAWKPNYHAHMIFDWTDGHTGKTVKLNRHDMAEMQTITAECLN
                     MERGVSSDKKHLSAMQYKNKMETEKAEQLQKDIEQLNRAYTAGTEKITTVQKELSTAQK
                     ELNSMKTDIHINEAKEAAAKAGKTVFNAVTSVFNVGEVKRQAKEIETLKKENYNLSFKN
                     QNLEGQLRTNNIEMRRVQETTDRQIKAEASNP"
                     /name="plasmid recombination protein"
     gene            4095..4343
                     /locus_tag="NXW55_22080"
     CDS             4095..4343
                     /locus_tag="NXW55_22080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="UVS02126.1"
                     /translation="MVTNIIQIGNSKGIILPSEVLKQLRLSLKSAVSISLDGNNIVIKA
                     QPRQGWAEAAKRAHENGDDELLIPDVFEDEKFEDWTW"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            4337..4657
                     /locus_tag="NXW55_22085"
     CDS             4337..4657
                     /locus_tag="NXW55_22085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="UVS02134.1"
                     /translation="MVEQYEVYWVELDPTRGGEMAKTRPCVVVTPSDLNMYLTTVVIVP
                     ITSTIRNYPYRVLCSVAGREGEIATDQIRTVDKSRLKRKIGDLNFSEIKRLREVFQQMF
                     CE"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            4694..5092
                     /locus_tag="NXW55_22090"
     CDS             4694..5092
                     /locus_tag="NXW55_22090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-poly-alpha-D-galacturonosidase"
                     /protein_id="UVS02127.1"
                     /translation="MKPTDYIEWDNLKDIPFFLCQVVEDREKQDLDIYYLGKRVLHDYD
                     HVGHYLRTAVILFRRVKSRTADWVNLRNLWTLRNCVRENYNHGIGMNDLIFGENFDGDN
                     LDTLTPLTKKRFDFLCKRIKELDPYATI"
                     /name="exo-poly-alpha-D-galacturonosidase"
     Typing          2..1049
                     /name="rep_cluster_870"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.714"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2746..3689)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.73"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtgcaaag atagaacaaa tataaccaac tgcaaagaaa gtccgttaaa aaacggttct
       61 gatagtctta aaaaaaaggc taaagtaaaa atcatatccc ataattttac tcgaaaactt
      121 tcggaaaaga atccggatag taagctgatg aaagcatata tggatgccca tgtgtgttca
      181 aatgtttata ctgtgcaggg aggtaaggcg cattcccatt attgtcatcg ggctttttgt
      241 cctatctgtc agcgtattca aacggcacaa aatgtaaaga agttccttcc ggttatgaag
      301 tatttggctt cggaaggcag agagttttat tttgtgacgc ttaccttgca aaattgtgta
      361 accgatgatg caagggtatt gcgagatttc atgcgtagat gtaaccggat gtgggctgat
      421 ggtataagga cgaaacacaa gtttcgttca cttggtattt cgggggtgat taagaaggag
      481 tgtacctatc atgtagtaaa aaaggatttg ttttcgttcc attatcattt tcatattatt
      541 gttgattcat tggaagctgc aaaatatgta gtagcccaat ggaagaaatt gcatggtagt
      601 acggttgctg atgcccgttt tcagaaatat aagaagatag aggattttga aaatgcagcg
      661 atagaggttt ttaagtatgc ttcaaaggct tctgtctcaa aatcgaaagg tcgtgacggt
      721 aaaaaaagat tcagataaat tataaggctt tggatatgat ttatactgcc atgcatggga
      781 tgcagcgcat gagtagtttc ggacagttcc gcaccatgat cggagatgaa gcgttgaatg
      841 attttagaga tgaggatttg gttcttaata ttgagggatt gaatgttgaa gatggttgtt
      901 atacttggta tatgtctgtg aaagataagg tggctgattg gtataatatg gaaactgggg
      961 aggcattgtg ttgttataag gtcacccgga aggatgaact tttgcaggat atttatttag
     1021 atgaggatat ttttcctaat agtgggtagt tgttagtttg ttgtattgtt ttcttggtat
     1081 ggtgtgagtt ctatgtattc tctattcatg aaaggatcgc caaattttcc acttctgtca
     1141 tagaaggaaa atttgttttt agttagctcg tctatgtccc agtaatgttc tccaccaaaa
     1201 taaatggtac tgaaacttgg atcaccgaat gaccattgtc cgtagatatt ggcttctttt
     1261 ttatttcctt gtttgtcgat ttctatattt gtacgggtat atgttccgtt tttattgaat
     1321 ttgtatgtac cttgccagac actgccgttt tcgtctgttg tttttttacc agcccatgta
     1381 atatcacaaa ttaatgagat tatgctttct tctccatggt tgtagtatcc ttgtttttca
     1441 acacatccgg tgtttgaaat aagaaaaagt gatactgttg aaataagtaa agagatatgt
     1501 attattctat tcatagttaa atatttaata ttcaatgcaa aaataggaga aattttatat
     1561 ttttgggcga aacctttatt tattaacttt aaaaaaggag gatttatgaa aaaagtaatg
     1621 tttatgttca tcagtctact gactgttttg ggtgtttgtt cgtgtaatga taatgaagta
     1681 atagaacctg tggttagtga ttctgtatct gatatggagg tcttatctcg ttttgttgat
     1741 gtaaatgaga ttacaaatga atactatttc aatgagaata agaaaacgag ggcgttgtct
     1801 tatgtaacag gttcggattg gcaggattta gaaaaagtta gtcctttgag tattgagaaa
     1861 tataagaata acctgcaggt tttaaatgct caagtggcaa gtgctatttc aaatcctaat
     1921 accgcttatg ttgttttttc tgtaaatgga aagactttgg ttaaaaaggt gaaggaggat
     1981 gctaattttg atttttcagt atttagggat gttgtaacgg aaacgagggc tgttctccct
     2041 tctttgagta ttaatggagg ttctcaaagc actaccggag ttttttacga ttccagtcgt
     2101 actttaaaga tgcaagttga tttgaatgct tcaatacaga ataattatta tttctttgaa
     2161 gtattaaatc ctaatgctaa accaagtccg gatgataata taacgacacc tgaaagtgtt
     2221 gcttttagtg gaactggacc gttatggagt aacactttca cttggacttc gtattgggat
     2281 gcaaatgtac ctggtcaagg ttttaaatgg gagtttaaag gaaaaggtac tactccttct
     2341 tttggtttta tagctaattg cacctttagt cgttaaaaaa tgaacgggat ttaatttgaa
     2401 ttgcacttct caaaaaagag aagtgcaatt ctttttagtg tctttctatc ggtgtagcca
     2461 gttaccgtta tctaaagtct tttgtagtgt attccaaagt tctttaagaa agtttttaaa
     2521 gtggatgtta tttagccaaa ttgtagtaat accattctga cttttggaga tatctatttt
     2581 cacgttttta acttggtatg attttctctt gtctttatcg taaaggtttc cagtgtagta
     2641 tatttccttt ccaataagaa gttgtcggat atctttattt tgtattccca tagtttggag
     2701 ttcctctata ttttcctgtg cattttccat ttccggaaaa aggttggtta tgggtttgat
     2761 gcttctgcct ttatctgtct gtcggttgtt tcctgtaccc ttcgcatctc gatgttgttg
     2821 gttcttaatt gtccttctaa attttgattc ttgaatgaga ggttataatt ttcttttttg
     2881 agggtttcaa tttctttggc ttgtcgtttt acttccccta cattaaagac gcttgtaacg
     2941 gcattaaata cggtttttcc ggcttttgcg gcagcttctt tggcttcgtt gatgtgtatg
     3001 tcggttttca tgctgtttag ctctttttgt gccgtactaa gttccttttg gactgtggtg
     3061 attttctcgg ttccggcagt gtatgccctg tttagttgct cgatgtcttt ttgaagttgt
     3121 tcagctttct ctgtttccat tttgtttttg tattgcatgg ccgacaagtg tttcttgtct
     3181 gaagaaactc cacgttccat gttgagacac tctgctgtta tagtctgcat ctccgccatg
     3241 tcgtgtctgt tcagctttac agtttttcct gtatggccgt ccgtccagtc aaagatcatg
     3301 tgcgcatgat agttcggttt ccatgcttct tcctttccgt cccatacgtt tgcgccttca
     3361 tccttatggg tgtagatttg gaaggcgtgc actccaaagc gttcttccag tttttcggcc
     3421 aagtcttgaa gctgctggat tgtggtttcc tcgctgatta ctattacccc ttcccggatt
     3481 ggggtagctt tcttctgtaa tccttgtccg gtggcttcct tgtatttttt cgttatgtcc
     3541 cgatgtactt cggcaatgga acattctacc cactgttcgt tgcggtgtgt gagttccgga
     3601 cggatgtagt cgagctcctt gcttcttaag ttgtgacgtt ccgagctgcc tatattgcag
     3661 gcttccactc tgatacatgt ctttgccata tctttaggtt ttaggtgtgt atgtctgttt
     3721 agaagatgtg gcgtgaaacg tcacatcgca cggggtcgag gggacacccc tcgcacacaa
     3781 cccctttagg ggttatagtg tgctataacc ttttgcaaag gtaagccttc cggacgggat
     3841 ttgcaagcgc tctgccatgt gttccggatg tgcttgggaa cagaagccgg acaaaaggga
     3901 aaaggacaca aggcacaata aaaaatcatc ggtcggggag cgctgcacgg caagggggtg
     3961 caccaacact cccgacgagt gcagccattg acagggttct cggtgaaaat ggaaaaaagt
     4021 tgtttttcct tttgttattg ttataacttt atatatattt gtatcgtgat aatcaattat
     4081 aaagttataa cattatggta acgaatatta ttcagattgg aaacagcaag gggataatac
     4141 ttccttccga agtattgaaa cagttgcgtt tgtccttgaa atcggctgtg agcatttctt
     4201 tggacgggaa caatattgtg ataaaggcac aaccacggca gggatgggcg gaagctgcca
     4261 aacgtgccca tgagaatggg gatgatgagt tgcttatccc tgatgtgttt gaggatgaaa
     4321 agtttgagga ttggacatgg tagaacagta tgaagtgtat tgggtagagt tagaccctac
     4381 ccgtgggggt gaaatggcaa agacacggcc atgtgtagtt gtcactcctt cagatttgaa
     4441 tatgtacctt acaacagtag tcattgttcc gataacctca acgataagaa attatcctta
     4501 ccgggttctg tgttctgtag ccgggcgtga aggagagata gccactgatc aaattcgcac
     4561 ggtggataaa agccggctga aaagaaagat tggagatttg aatttcagtg agataaaaag
     4621 gttacgtgaa gtatttcagc aaatgttctg tgaataatgg aaagtacgga aagaaaaatg
     4681 caaataagac attatgaaac caactgacta catcgaatgg gataacctga aggatattcc
     4741 attcttcctc tgtcaagtag tcgaggacag agaaaaacaa gatttggata tttactattt
     4801 gggcaaacgt gtcttgcacg attacgacca tgtggggcac tatttgcgga ctgccgttat
     4861 cttgttccgc agggtaaaga gccgcacagc agactgggta aacctgcgta acctttggac
     4921 gctgcgcaac tgtgtgcgtg agaactacaa tcacggcata ggaatgaacg accttatttt
     4981 tggggagaat ttcgatggag ataatctcga tacgcttaca ccacttacaa agaaacgctt
     5041 tgattttttg tgtaagcgga ttaaggaact tgacccatat gcaactattt aacactccat
     5101 tgagtgaagg cagggcttgt ctttgtgtgc tccccctttg ggggacggga gggggataag
     5161 aaaaaaatac caccggagat gtcggatatc cgtcaattca ctccggtgtt ttttttgcaa
     5221 ctctcccgta tcgctcggca agggttgtct ttgtgtacgg caaagatagt tttttttccg
     5281 gaagtatctt tgtctgttgt ggatttagta gtgtatttgt gtcagaattg cgcagccggg
     5341 ggaatggggg cgagccccca tactagataa actagaaagt ttcgtggtaa atgctttttg
     5401 acttgttata tgtaggtttt ttgaatgatg tttggcggtt tggaatgaat tacctacttt
     5461 tgggctgaga aaaaacgaaa gcccgaaggc agtaaccaac gggctttgta ttttaagaat
     5521 tagcaatcgt ttgtgttcgt tcttggcggg gcggatacca aacgaaagcc acatcaaaat
     5581 ttcat
//
