LOCUS       CP103227                2702 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-604 plasmid unnamed2, complete
            sequence.
ACCESSION   CP103227
VERSION     CP103227.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323482
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 132x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 21:47:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,267
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 4,121
            CDSs (with protein)               :: 4,121
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 32 of 55
            Pseudo Genes (incomplete)         :: 25 of 55
            Pseudo Genes (internal stop)      :: 9 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-604"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="2014-11-14"
     gene            1..1104
                     /locus_tag="NXV67_21330"
     CDS             1..1104
                     /locus_tag="NXV67_21330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="UVR84808.1"
                     /translation="MGATSIHVQAVKPGSEIHNFREKELDYVRPELSHLNESWVGDSIS
                     HRLESAKQRYLDTVGQKMQAKAAPIREGVIVIKQETTMQELQQFATVCKERFGIEAFQI
                     HIHKDEGYMNAKQWTPNLHAHVVFDWTQPNGKSVRLSRDDMAELQTIASETLGMERGVS
                     SDRKHLSAMQYKTECAKEQLQELSNDISSALDKHKDVQNQLLQLQKELRSIETKKNVQK
                     LISKASEKFYGLIGKTVNDREKDALKAKVKALEGENEQLSDRLGKAILEKEQNGTKAFK
                     AENDKEYYRQQMDNARTTSNRLRTENQELKTETKELKKELGKMKDLFNSEQLEALRHHF
                     PNISKAMEEGKDLLKQITRSRGFGMGM"
                     /name="mobilization protein"
     gene            1543..2133
                     /locus_tag="NXV67_21335"
     CDS             1543..2133
                     /locus_tag="NXV67_21335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="UVR84807.1"
                     /translation="MENKKAVKLTDFQKNEENPFMKQAIEGIENHVVKKYKSNSGGDKR
                     AVVALADTETGEVFKTSFIRQIEVDEEQFTKLYLSNFAAFFDLSQAAIRVFGYFMTCMK
                     PKNDLIIFNRKKCLEYTKYKTDKAVYKGLAELVKAEIIARGPADNLWFINPLIVFNGDR
                     VTFAKTYVRKKTLAAQKKEEAEKRQLSLGFDEQ"
                     /name="replication/maintenance protein RepL"
     Typing          1511..2123
                     /name="rep_cluster_1868"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.701"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..1101
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.275"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgggagcaa caagtattca tgtacaagca gtgaagccgg ggagtgagat tcacaacttt
       61 agggaaaaag agttggacta tgttcgtccc gaacttagtc atttgaatga aagctgggtt
      121 ggagatagca tctcccatcg gctggaaagt gcgaaacaaa gatatctcga tacggttggg
      181 cagaagatgc aggctaaagc cgcacccata cgagagggag taatagtaat caaacaagaa
      241 accaccatgc aggaactcca gcagtttgcc acggtctgca aagaacgttt cggtatcgaa
      301 gcatttcaaa tccatataca caaggacgaa ggatacatga acgcaaagca atggacacct
      361 aaccttcatg cccatgtagt tttcgattgg acgcagccga atgggaaaag tgtgcgttta
      421 tcgcgtgatg acatggcaga actccaaacc atagcatctg aaacactggg catggaacgt
      481 ggcgtttctt ctgaccgcaa acacttatcg gctatgcagt acaagaccga atgtgcgaaa
      541 gaacagcttc aggaactatc aaacgatata tccagtgcat tagacaaaca caaggacgta
      601 caaaaccagc ttcttcagct tcagaaagaa ctacgttcca ttgaaacaaa gaagaacgtc
      661 caaaaactca tttctaaggc ttcagagaag ttttacggct tgattggaaa aacagtcaac
      721 gatagggaaa aggatgcctt aaaagccaaa gtaaaggctt tagaggggga aaatgaacag
      781 ctatccgata gactgggaaa ggctatactt gaaaaagagc aaaacggcac aaaggcattc
      841 aaagccgaga atgacaagga gtattataga caacaaatgg acaacgcaag gacaaccagt
      901 aaccgattaa ggacggagaa ccaagaacta aagaccgaaa ccaaggaatt gaaaaaagaa
      961 cttggtaaga tgaaagactt gttcaattcc gaacaactgg aggctttaag gcatcatttc
     1021 ccaaacatat caaaagctat ggaagagggg aaagacctac tcaagcaaat caccagaagc
     1081 agaggtttcg gtatgggtat gtaacccccc cccgccccca aagggggagc gaccaaacgg
     1141 cagcctctct caatggagtg ttacgtcgtt cggattcgga agaaattttg cagggttcgg
     1201 aaaacggttg tgtgcttatt ctaaaaaaag tttatggtgt ggcgctcgtc gggacgggtg
     1261 gtcccgcccc ttgccgctgg gcgctccccg accgatgatt tttaagtgac tgattttgtg
     1321 ctgttttggg ggtatattaa gaatagaaga aatagaataa gttaagtact tgatacacaa
     1381 tatagggcat tttccatatt ggaaattctc attttccaat ctagaaaata ctgatttcct
     1441 aatataatac taaacaggaa aataatattt cccttaatat tgtttttatg gaaaataata
     1501 gtttactttg tggagaataa tatttcccaa aaacacatca aaatggaaaa taaaaaagca
     1561 gttaagttaa ccgattttca aaagaacgaa gaaaatcctt ttatgaaaca agctatagaa
     1621 ggtattgaaa atcatgttgt taaaaagtat aagagtaata gtggtggcga taagagagcc
     1681 gtagtagctt tagccgacac tgaaactgga gaagtgttta agacttcgtt tatccgtcaa
     1741 atagaagtag atgaagaaca attcactaaa ttgtatcttt ctaactttgc tgcattcttt
     1801 gacctatcac aagcagctat tcgggttttt ggttacttta tgacctgcat gaaacccaaa
     1861 aatgatttaa tcatcttcaa tagaaaaaaa tgcctagaat ataccaaata caaaacagac
     1921 aaagccgttt ataaaggact tgcagaactt gtaaaagctg aaatcatagc ccgaggacca
     1981 gccgataatc tttggtttat taatcctctg atagtattca atggtgaccg agtgacattt
     2041 gctaaaacat acgttcggaa aaagacttta gctgcccaaa agaaagaaga agcagagaaa
     2101 cgacaattat cacttggctt tgatgaacag taacactcca ttgagtgaag ctgctctatg
     2161 taaagttata atgggggatg aaaggcaagg ttcgctaacc ttaccccgat tgtttatgct
     2221 tcccagcatt caatctatcc ccgtttatgg gtttctcttt caccctacaa agataacctc
     2281 atgggggaaa aatgtccaag aatatggggt aaaaactatc aagtcggtag aaaataagta
     2341 tctttgaagt acatttttag tagaggtact ggtatgccta gacgaagcag ataggcaaaa
     2401 ataaagccgc tggagatgtc ggatgtccgt caattaactc cagcggcttg tattttggta
     2461 actctcccgt atcggtcggc taccgtttgg tcagcaacaa aggtagtact ttattgtcgc
     2521 aaatccaaat gttttcccct ttatttgtgc atttacagaa catttgctaa cttcgcacct
     2581 atgcagtatg gtgatagcac actagcaccc taaagggtgc gtgtgtaagg ggacaagccc
     2641 ccttaacccc ctgtcagccg gctgtcaccg gctgttatcg attgtcacaa aaatgttaac
     2701 tt
//
