LOCUS       CP103226                4462 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-604 plasmid unnamed1, complete
            sequence.
ACCESSION   CP103226
VERSION     CP103226.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323482
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 132x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 21:47:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,267
            CDSs (total)                      :: 4,176
            Genes (coding)                    :: 4,121
            CDSs (with protein)               :: 4,121
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 32 of 55
            Pseudo Genes (incomplete)         :: 25 of 55
            Pseudo Genes (internal stop)      :: 9 of 55
            Pseudo Genes (multiple problems)  :: 9 of 55
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-604"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="2014-11-14"
     gene            1..450
                     /locus_tag="NXV67_21290"
     CDS             1..450
                     /locus_tag="NXV67_21290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR84803.1"
                     /translation="METNSRPEKEVSTSKQGQYRPRRKDTTPKVNPQLAVELVYQELKR
                     VEAYTKRIEDATAKKVQIDGENFESAEKRLKNVLSDFERQGNRMKNGGYVDKRVSFYSI
                     LCAVISLLFACLMCYLWVDAAKDRDNYKRYYEYYQEQAIEQKGNK"
                     /name="hypothetical protein"
     gene            complement(486..1181)
                     /locus_tag="NXV67_21295"
     CDS             complement(486..1181)
                     /locus_tag="NXV67_21295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004384494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR84804.1"
                     /translation="MKKKNSLLFILLMYSLTMLAQKDITKFMGIPVDGFKKDMIQKLKA
                     KGFEYDNEIDLLTGEFNGEKVNIFIATQSNKVWRIVVADAIERNEHDIKIRFNNLYDQF
                     NDNPKYVPKLEDNDYISEDINLAYEMKVRNKRFEAGFMQMTNPKSPQNSPEKIQQELTQ
                     KISEICPTEEFIRKSEKEKEDITKEAAMNIVQEAAMRSVWFMISEKYGKFSLILFYDNE
                     YNNAHGEDL"
                     /name="hypothetical protein"
     gene            complement(1262..2287)
                     /locus_tag="NXV67_21300"
     CDS             complement(1262..2287)
                     /locus_tag="NXV67_21300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UVR84805.1"
                     /translation="MKKKLPITKNKDVVVSWVYTWSKQQDMSIHEQRIVLRILEACQAE
                     LKGVKLKDYAGTKRKFEHGLWDVDAQMHVSDVIFSGRDYNEIIAALDSLAGRFFTYEDD
                     EEWWKCGFISNPKYKKHTGIITFRVSNDLWDVFTKFAKGYREFELNKALALPTGYSLRF
                     YMLMSGQVYPLDISLDNLKERLGIPADKYKDKNGKDRIDNFEERVLKPAKAALDESCPY
                     TFNYVKVRENPNNKRSKVTGFRFYPVYQPQFRDEELEVKELQAKVTARHQIDSHVYEYL
                     RYSCGFTSEEINRNKETLITAQEKIADLIGELAILNGKSREKNNPKGWIINSLKGKIKD
                     S"
                     /name="replication initiation protein"
     gene            2488..2745
                     /locus_tag="NXV67_21305"
     CDS             2488..2745
                     /locus_tag="NXV67_21305"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR84806.1"
                     /translation="MLITDKGNLKGKRLKINSKRGEKAKQHQPTGGRNEIYALVKVGRE
                     DLMITPELQARIDKAREEIKSGKCVTLKSSEDIDAYFDSL"
                     /name="hypothetical protein"
     gene            2742..3017
                     /locus_tag="NXV67_21310"
     CDS             2742..3017
                     /locus_tag="NXV67_21310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004519 - endonuclease activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family toxin"
                     /protein_id="UVR84800.1"
                     /translation="MMYTIRVSDGVDKVIAKWKKSNPNLFKKYKKIYKELLEHPKTGLG
                     HPEALRGGGDITWSRHITAHDRIIYDIYEEVVEVYILEVEGHYNDK"
                     /name="type II toxin-antitoxin system YoeB family toxin"
     gene            3028..3224
                     /locus_tag="NXV67_21315"
                     /pseudo=""
     CDS             3028..3224
                     /locus_tag="NXV67_21315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            3417..3716
                     /locus_tag="NXV67_21320"
     CDS             3417..3716
                     /locus_tag="NXV67_21320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR84801.1"
                     /translation="MEVRRNEKITFRCSALEKAALSEQAARCGLSTSEYCRSLSLGGRP
                     RERYTEEERELLRDIARLKGTLQRLNNYFGGRQYREVFEENRALITELKKILSR"
                     /name="hypothetical protein"
     gene            3713..4438
                     /locus_tag="NXV67_21325"
     CDS             3713..4438
                     /locus_tag="NXV67_21325"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UVR84802.1"
                     /translation="MIGKGKSISHGVAALEYDLAKEIDGQAAATEIARHELYGCTGAEM
                     VQEMKPYFTDFPNVKNNCLRFEVSPSIEESSSFTDTDWAELGNDFMQRMGLMNHQYIII
                     KHSGTESKKKQAHLHILANRISLSGELYRDNWIGKRATEAANAIAKERDFVQSQDIGKA
                     NKAEIKEAMNEVLKKLQGFDLAKFQRNLANWDSKFGKPGQAPENLTATMSQPGAERNTR
                     QARLGKVTHSPISSRHRKN"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          3714..4285
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="80.628"
                     /coverage="94.0"
                     /evalue="3.12e-118"
ORIGIN
        1 atggaaacaa actcacgtcc ggaaaaggag gtttccacct ctaagcaagg acaataccga
       61 ccacgcagga aagacaccac cccgaaagtg aacccgcagc ttgcagtaga actggtatat
      121 caagaattga agcgcgtgga agcctatacg aaacgcatag aggacgcaac agccaaaaag
      181 gtacagatag acggggagaa ctttgaaagt gccgaaaaac gcctaaaaaa cgtgttgtcg
      241 gactttgaac ggcaggggaa tagaatgaaa aatggtggct atgtggataa acgggtttcg
      301 ttctactcca ttctttgtgc tgttatttcc cttttgttcg cttgcctcat gtgttatctt
      361 tgggtggatg cagccaaaga tcgggacaac tacaaacggt attacgaata ttaccaagag
      421 caggcaatag aacaaaaggg caacaaatag acgaagtaca cttttgtact tcgtctattt
      481 gttttttaga ggtcctcccc atgagcatta ttatattcgt tatcgtaaaa aagaattagt
      541 gagaatttgc catatttttc actaatcata aaccatacgg agcgcattgc agcttcttgt
      601 acgatgttca ttgctgcttc tttggttatg tcctcttttt ctttttctga ttttcttata
      661 aattcttcgg ttggacatat ctcgcttatt ttttgagtta attcttgttg tatcttttct
      721 ggtgagtttt gaggactttt gggatttgtc atttgcatga atccagcttc aaatcttttg
      781 tttctaacct tcatttcata tgcaagatta atgtcttcag aaatataatc gttatcttcc
      841 aatttaggaa cgtatttagg gttatcgttg aattggtcgt aaaggttatt gaatctaatt
      901 ttaatatcat gctcgttcct ttctatcgca tcagctacga caatcctcca tactttattg
      961 ctttgagtgg cgataaaaat atttactttt tcgccattaa attcaccagt taaaagatcg
     1021 atttcattgt cgtattcaaa ccctttagct ttaagtttct gaatcatatc ttttttaaat
     1081 ccatctacgg ggattcccat aaattttgtt atatctttct gtgctaacat tgtcaaagag
     1141 tacattagca gtataaataa cagtgaattt ttcttcttca tgaataaata tttaaagatt
     1201 acaattgcaa atttagaaaa tgatttgaaa atatgacatt tagggaagca cttttagttg
     1261 cttagctgtc ttttattttc cctttgagag agtttataat ccacccttta gggttgttct
     1321 tctctcgtga ttttccgttt agaatagcca gttctcctat aaggtcggct attttttcct
     1381 gtgcagttat aagcgtttcc ttgttccggt ttatttcttc cgaagtaaaa ccacaggaat
     1441 aacggagata ttcgtacaca tgactatcta tttggtggcg tgccgttact ttcgcttgaa
     1501 gttcttttac ttccagttct tcatccctga attgaggttg atatacaggg taaaatctaa
     1561 atccagttac tttactacgt ttgttgttag ggttttcacg cacttttacg tagttgaatg
     1621 tgtatgggca gctttcatca agcgcagcct ttgctggttt taatactctt tcctcgaagt
     1681 tgtcgattct atcttttccg tttttgtctt tgtatttgtc cgcaggtatg ccaagtcgtt
     1741 cttttaagtt gtccagtgat atatccaaag ggtacacctg cccactcatg agcatataaa
     1801 acctaagtga atagcctgta ggcagcgcaa gagccttgtt tagttcaaat tccctatatc
     1861 cttttgcgaa tttggtaaac acgtcccaaa ggtcattaga tacacgaaat gttataatac
     1921 cggtatgctt tttgtatttc gggttagata taaatccaca tttccaccat tcctcatcat
     1981 cttcgtaagt gaaaaaacgt ccagccagtg aatcgagtgc agctatgatt tcgttgtagt
     2041 cacgtccgga aaaaataacg tcagacacat gcatttgcgc atcaacatcc caaagtccat
     2101 gctcaaattt gcgtttcgtg cctgcataat ctttcagctt tactcccttt agttcagcct
     2161 ggcaggcttc cagtatgcga agaactattc tttgttcgtg tatggacatg tcttgctgtt
     2221 tggaccatgt atatacccat gatacaacta cgtctttatt tttggtaata ggtagcttct
     2281 ttttcataac tatttgattt gctgcaaaga tataaaatac ctacatataa acaaaaaaag
     2341 aagggtaaaa ctcccaaagt gtagggaaag ttcgtcttaa aaactcccaa agtgtaggat
     2401 taaaactccc aaagtgtagg gaaacacatg ttttaatccc tttatttatc ggtgtttccg
     2461 caaaagtgat agagcatatt acagacgatg cttataacag acaaggggaa tttaaaggga
     2521 aaaaggctga aaatcaactc gaaaagaggg gaaaaagcaa agcaacacca accaacgggc
     2581 ggtaggaacg agatttacgc tttggtaaaa gttggtcgtg aagatttgat gataaccccg
     2641 gagctgcaag caaggattga caaggcaagg gaagaaatca aatccggaaa gtgtgttacc
     2701 ctcaaaagca gtgaggatat tgacgcttat ttcgatagtc tatgatgtac acaattaggg
     2761 tttccgacgg ggtggataag gtgattgcca aatggaagaa atcaaacccc aatttgttca
     2821 agaagtataa gaaaatctac aaagaattgt tagagcaccc taagactggg cttgggcatc
     2881 ccgaagcgtt aaggggcggt ggggatatta catggtctcg acacattacc gcccatgacc
     2941 ggataatcta tgacatttac gaggaagtgg tagaggtcta catcttggag gtagaggggc
     3001 attacaacga taaatgataa aactgttatg gtcgaatatt gtgtttactg gttagagaac
     3061 ggcgagccta tgcacgaggt gttctcttct cttgccgccg ccgagatgta ctcatgtgcg
     3121 atagagggaa agaaaacatt gaatgggtgg aggtgtccga agaagaaacc attgatttgg
     3181 atgaactgga agacatgttc ccggatgact tctgcggcgt gtaatcccct tgggtagaaa
     3241 tggggtagaa tttgttaggc tgtgggggat tttctaacac gccggggctt tttgctgggc
     3301 ggttggcgtg gatgtattcc catgcggcat gtgtatatat agcaagaagt gtccttgtcg
     3361 gacaattctt gcttttctcg ctttgctcaa aaagattttt tgcctatctt cgtgacatgg
     3421 aagtaagacg gaacgaaaag ataaccttcc gatgctccgc acttgaaaag gcggcacttt
     3481 cggagcaggc agcccggtgc ggtctgagca catcggagta ctgcaggagt ctctcccttg
     3541 gtggtcgccc cagggagagg tacacagaag aagaacggga acttctccgg gacatcgccc
     3601 ggctgaaggg gacacttcag agactgaaca actacttcgg tggtcgccag taccgggaag
     3661 tgttcgagga gaaccgggca ctaattacag agctcaaaaa aatactctca cgatgatagg
     3721 gaaagggaaa agcatatcac atggcgtggc ggcactggag tacgacctcg ccaaagagat
     3781 agacgggcag gcagcagcca ccgaaatagc tcggcatgag ctttacgggt gtacgggtgc
     3841 ggaaatggtg caggagatga aaccttactt cactgatttt ccgaacgtaa agaacaactg
     3901 cctgcgtttt gaggtcagcc cctcgataga ggaaagctcc agcttcaccg atacggactg
     3961 ggcagaactt ggcaacgact tcatgcagcg catgggactg atgaaccacc agtacatcat
     4021 catcaaacac agtgggacgg agagcaagaa gaaacaggcg cacctgcata tactggcaaa
     4081 ccggatatcc ctttccgggg aactctatcg ggacaactgg atagggaaac gggcaacgga
     4141 agccgccaac gccatagcca aggaacggga ctttgtgcag tcgcaggaca tcggaaaagc
     4201 caataaagcg gaaatcaagg aagccatgaa cgaggtactg aagaagctgc agggctttga
     4261 ccttgccaaa ttccaaagga acttggcaaa ctgggattca aagttcggga agccagggca
     4321 agcaccggaa aacttaacgg ctactatgtc acagcccgga gcggaacgga atacaaggca
     4381 agcgagattg ggaaaggtta cacactcgcc catatcgagc agacacagaa aaaattgaag
     4441 tataaccaaa tgagcatatc ac
//
