LOCUS       CP103221                4253 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-502 plasmid unnamed3, complete
            sequence.
ACCESSION   CP103221
VERSION     CP103221.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323405
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 464x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 22:05:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,210
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 32 of 61
            Pseudo Genes (incomplete)         :: 33 of 61
            Pseudo Genes (internal stop)      :: 11 of 61
            Pseudo Genes (multiple problems)  :: 14 of 61
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-502"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="2006-03-01"
     gene            join(4118..4253,1..50)
                     /locus_tag="NXX63_21495"
     CDS             join(4118..4253,1..50)
                     /locus_tag="NXX63_21495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR72214.1"
                     /translation="MKKIIVHFKKEEFERVKNAISILAKFSEIYAPHIDKANSILSFRV
                     DDENETTEEQQCNSRQ"
                     /name="hypothetical protein"
     gene            1..1005
                     /locus_tag="NXX63_21500"
     CDS             1..1005
                     /locus_tag="NXX63_21500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UVR72215.1"
                     /translation="MMKTKQQKNNSVTVVNKDQIQSYIMTTAKYDFSVYEKRILYRIVE
                     MAQGELQGVRFATDCKKIEHTLFDTVHITMPIASLLNGESDENYTRVKQALLSLQKKIF
                     TYEDEKEWISISIIALPKIKKRSSVVSFQIDSHIWDCCLDFSKGFRKYELATAMKFKSV
                     YSMRFYELLSGQKTKLIYPLEQLKEMFKVQDKYAKTNDFVRKVIEPAKNELDELSPYTF
                     EWSANKEGKKIVSFNFYPIFKPEHRDAELYKKELQKQTGLSWDLGRQVISYLKTSLEFS
                     DKEIKNNRDLFVTAQMELPDIMTELAILRGKSRTKTNPKGWIINALKWKIKDK"
                     /name="replication initiation protein"
     gene            complement(1044..1274)
                     /locus_tag="NXX63_21505"
     CDS             complement(1044..1274)
                     /locus_tag="NXX63_21505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="charged multivesicular body protein 2b-B"
                     /protein_id="UVR72216.1"
                     /translation="MGTAEHEEPRFFFILNKGAKSGGEITHAVLNGSIVSKPAGWDAFH
                     GLVLAKEKLSSVEIQRQMKELGVEMEIVPLI"
                     /name="charged multivesicular body protein 2b-B"
     gene            complement(1294..1746)
                     /locus_tag="NXX63_21510"
     CDS             complement(1294..1746)
                     /locus_tag="NXX63_21510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR72217.1"
                     /translation="METNSHPEAAASNVKQGQYSPRKRKDTTPRVNPQLAVELVYQELK
                     RVEVYTKRIEDATARKVQIDGKSLESAENRLKNVLADFERQGYRMKNGGYVDKRISFYS
                     ILCAVISLLFACFMCYLWTDAAKDRDNYKQYYEYYQEQAREQKGNK"
                     /name="hypothetical protein"
     gene            complement(1703..2497)
                     /locus_tag="NXX63_21515"
     CDS             complement(1703..2497)
                     /locus_tag="NXX63_21515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UVR72218.1"
                     /translation="MIGKGKSISHGVAALEYDLAKEINGQAVATEIARHELYGCTGAEM
                     VQEMKPYHIDFPNVKNNCLRFEVSPSIEESATFTDADWAELGNDFMQRMGLANHQYIII
                     RHSGTESKKEQAHLHILANRVSLSGELYRDNWIGKKATEAANAIAKERNFVQSQDIGKV
                     NKAEIKEAMDGVLKKMQGFDFTKFKEELGKRGFKVREARASTGKLNGYYVTARSGTEYK
                     ASEIGKGYTLAHIERTQSKLKCNSMNISHGNKLTPGSGSFQR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2922..3119)
                     /locus_tag="NXX63_21520"
     CDS             complement(2922..3119)
                     /locus_tag="NXX63_21520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR72211.1"
                     /translation="MVEYCVYWLENGEPMHEVFSNLAAAEMYSCAIRGKENVEWVEVSE
                     EEAIDLDELKDMFPDDFCGV"
                     /name="hypothetical protein"
     gene            complement(3130..3405)
                     /locus_tag="NXX63_21525"
     CDS             complement(3130..3405)
                     /locus_tag="NXX63_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004519 - endonuclease activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family toxin"
                     /protein_id="UVR72212.1"
                     /translation="MMYTIRVSDGVDKVIAKWKKSNPNLFKKYKKIYKELLEHPKTGLG
                     HPEALRGGGDITWSRHITAHDRIIYDIYEEVVEVYILEVEGHYNDK"
                     /name="type II toxin-antitoxin system YoeB family toxin"
     gene            complement(3402..3653)
                     /locus_tag="NXX63_21530"
     CDS             complement(3402..3653)
                     /locus_tag="NXX63_21530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR72213.1"
                     /translation="MEALSVREYRNNLAASFTKADNGEQVLIRRKNEIYALVKVGREDL
                     MITPELQARIDKAREEIKSGKCVTLKSSEDIDAYFDSL"
                     /name="hypothetical protein"
     Typing          2..1005
                     /name="rep_cluster_1019"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgatgaaaa cgaaacaaca gaagaacaac agtgtaacag tcgtcaataa agaccaaata
       61 caaagctata ttatgacgac agccaagtat gacttttccg tttatgagaa gcgcatactt
      121 tatagaattg tagaaatggc tcaaggtgaa ctccaaggag ttagatttgc aactgattgt
      181 aaaaaaatag aacacacact ttttgatacc gttcacatca caatgcctat tgcttcattg
      241 ctaaatggag aaagtgatga aaactacaca cgtgtaaaac aagcactgtt atctctacaa
      301 aagaaaatct ttacctatga ggatgaaaaa gaatggataa gcatttctat tattgcattg
      361 ccaaagataa agaagcgttc atctgttgta tcattccaaa tagactcaca catatgggac
      421 tgctgcttag atttttcaaa agggtttcgc aaatacgaat tagccactgc gatgaagttt
      481 aagagtgtat attctatgcg gttctacgaa cttttaagcg gtcagaaaac aaaactaatc
      541 tatccacttg aacagctcaa agaaatgttt aaagtacaag ataaatacgc aaagacaaac
      601 gattttgtcc ggaaagtaat tgaacctgca aaaaatgagc tggacgaact aagtccttat
      661 acattcgaat ggtccgcaaa caaggaaggg aaaaaaattg taagtttcaa cttctatcca
      721 atattcaagc cagagcatcg tgatgcagag ctatacaaaa aagagctaca aaagcaaacc
      781 gggctttcat gggatttagg taggcaagtg ataagttact taaaaacatc tttggagttc
      841 tccgataaag aaatcaaaaa caaccgggat ttatttgtaa cagcacaaat ggaacttcca
      901 gacatcatga cagaactcgc catacttaga ggaaaatcaa ggacaaaaac aaatccaaaa
      961 gggtggatta taaatgccct taaatggaaa atcaaggata agtgacaaga ttaccccggc
     1021 tgaaatatac cggggtaatc ttgtcaaatc agaggaacga tttccatttc tacaccaagc
     1081 tctttcatct gccgctgtat ttccacgctg cttagtttct ctttcgccag tacaagccca
     1141 tgaaaggcgt cccagcctgc cggcttcgaa acgatactac cgtttaggac ggcatgggtg
     1201 atttcgccgc cggacttcgc acccttgttc agtatgaaga agaaccgggg ttcttcgtgt
     1261 tccgctgttc ccataattcg taaagttaga tgtttattta ttcccctttt gttcccttgc
     1321 ctgctcttgg tagtattcat aatactgctt gtagttgtcc cggtctttgg ctgcatccgt
     1381 ccaaagatag cacatgaaac aagcgaacaa tagggagata acagcacaaa ggattgagta
     1441 gaacgaaatt cgtttgtcca cataaccgcc gtttttcatt ctatacccct gccgttcaaa
     1501 gtccgccaat acgtttttta ggcgattttc ggcactttca aggcttttcc cgtctatttg
     1561 tacctttcgg gctgttgcgt cctctatgcg cttcgtatag acttcaacac gctttagttc
     1621 ttgatacacc agttctaccg caagctgcgg gtttaccctt ggcgttgtgt cttttctttt
     1681 ccgggggctg tactgtccct gcttaacgtt ggaagctgcc gcttccgggt gtgagtttgt
     1741 ttccatgaga tatgttcatt gagttgcact tcagtttgct ttgtgtccgc tcgatatggg
     1801 cgagagtgta gcctttccct atctcgcttg ccttatactc cgtaccgctt cgggctgtga
     1861 catagtagcc gttaagtttc ccggtgcttg ccctggcttc ccggacttta aaacctctct
     1921 taccaagttc ttccttgaat ttggtaaagt caaagccctg catcttcttc aatacgccgt
     1981 ccatggcttc cttgatttcc gctttgttga ctttgccaat gtcctgcgac tgtacaaagt
     2041 tccgttcctt ggctatggca ttggcggctt ccgttgcctt tttccctatc cagttgtccc
     2101 ggtacagttc cccggaaagg gaaacccggt ttgccagtat gtgcaggtgg gcttgctctt
     2161 tcttgctctc cgtaccgctg tgccggatga tgatgtactg gtggttcgcc aaccccatgc
     2221 gctgcatgaa gtcgttacca agttccgccc agtccgcatc cgtaaaggtg gcgctttcct
     2281 ctatcgaggg gctgacctcg aaacgtaggc agttgttttt tacgttcgga aaatcaatgt
     2341 gatacggttt catttcctgc accatttccg caccagtaca cccataaagt tcatgccggg
     2401 ctatctcggt ggctactgcc tgcccgttta tctctttggc gaggtcgtac tccaatgccg
     2461 ccacaccatg tgatatgctt ttccctttcc ctatcatcgt gagagtattt ttttgagctc
     2521 tgtaattagt gcccggttct cctcgaacac ttcccggtac tggcgaccac cgaagtagtt
     2581 gttcagccgt tggagcgttc ctttcaactg ggctatgtcc cggagaagct gccgttcctc
     2641 ctcggtgtac ctctcccttg ggcgcccccc aagggacaaa ctccggcagt attccgatac
     2701 gcttagactg caccgggctg cctgctccgc aagtgcgtcc ttttcaagtt cggtacatcg
     2761 gaatgtaatc ttttcgttcc gtcttagttc catgccacaa aggtaatctt aaaatctttt
     2821 atacacatgc cgcatgggaa tacatccacg ccaaccgccc ggcaaaaagc ccaggcatgt
     2881 tagaaaatcc cccacagcct aacaatttct acccaagggg attacacgcc gcagaagtca
     2941 tccgggaaca tgtctttcag ttcgtccaaa tcaatggctt cttcttcgga cacctccacc
     3001 cattcaacgt tttctttccc tcttatcgca catgagtaca tctcggcggc ggcaaggtta
     3061 gagaacacct cgtgcatagg ctcgccgttc tctaaccagt aaacacaata ttcgaccata
     3121 acagttttat catttatcgt tgtaatgccc ctctacctcc aaaatgtaga cctctaccac
     3181 ttcctcgtaa atgtcataga ttatccggtc atgggcggta atgtgtcgag accatgtaat
     3241 atccccaccg ccccttaacg cttcgggatg cccaagccca gtcttagggt gctctaacaa
     3301 ttctttgtag attttcttat acttcttgaa caaattgggg tttgatttct tccatttggc
     3361 aatcacctta tccaccccgt cggaaaccct aattgtgtac atcatagact gtcgaaataa
     3421 gcatcaatat cctcactgct tttgagggta acacactttc cggatttgat ttcttccctt
     3481 gccttgtcaa tccttgcttg cagttccggg gttatcatca aatcttcacg accaactttt
     3541 accaaagcgt aaatctcgtt tttcctgcga attagtacct gctcgccatt gtcagctttg
     3601 gtgaaagacg ctgcgaggtt gttacggtat tctcttactg ataatgcttc catatctgtt
     3661 tgcttttaaa ttcagtacaa agatagctat atttcaataa aatacaaact ttttgcacac
     3721 aaatgtgtac acgccataaa aagccgttca cagtcctacc gcccgttggt tggttttgct
     3781 ttgctttttt cccctattgt ttttcttttt ccgacagtaa atcaaccact tatccgtaaa
     3841 aaatcaatga cttgttataa agcataactt gtttatatgc ttatcagctt ttttttttaa
     3901 ctaaaaatta tgctgaaatc caataattta cacaatctat ttaacacatt actacgactt
     3961 tttgaatgct atttaacaca ttactacgac tttttgaatg ttattttccc ttccaaatac
     4021 gactttttga atgttatttt gcatcatcta cgattttcat ttgcacaagt cgtaattact
     4081 tattatattt gcgcaacata ctaataatca ataaaggatg aagaaaatca tagttcattt
     4141 taagaaagaa gaatttgaaa gggttaaaaa cgcaatttct atcctcgcaa aattttctga
     4201 aatatacgct cctcacatag ataaagccaa cagtatttta tcctttcgtg tag
//
