LOCUS       CP103220                5574 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-502 plasmid unnamed2, complete
            sequence.
ACCESSION   CP103220
VERSION     CP103220.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323405
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 464x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 22:05:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,306
            CDSs (total)                      :: 4,210
            Genes (coding)                    :: 4,149
            CDSs (with protein)               :: 4,149
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 32 of 61
            Pseudo Genes (incomplete)         :: 33 of 61
            Pseudo Genes (internal stop)      :: 11 of 61
            Pseudo Genes (multiple problems)  :: 14 of 61
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-502"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="2006-03-01"
     gene            148..1050
                     /locus_tag="NXX63_21455"
     CDS             148..1050
                     /locus_tag="NXX63_21455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="UVR72207.1"
                     /translation="MKAYMDAHVCSNVYTVQGGKAHSHYCHRAFCPICQRIQTAQNVKK
                     FLPVMKYLASEGREFYFVTLTLQNCVTDDARVLRDFMRRCNRMWADGIRTKHKFRSLGI
                     SGVIKKECTYHVVKKDLFSFHYHFHIIVDSLEAAKYVVAQWKKLHGSTVADARFQKYKK
                     IEDFENAAIEVFKYASKASVSKSKGRDGKKKIQINYKALDMIYTAMHGMQRMSSFGQFR
                     TMIGDEALNDFRDEDLVLNIEGLNVEDGCYTWYMSVKDKVADWYNMETGEALCCYKVTR
                     KDELLQDIYLDEDIFPNSG"
                     /name="protein rep"
     gene            complement(1054..1515)
                     /locus_tag="NXX63_21460"
     CDS             complement(1054..1515)
                     /locus_tag="NXX63_21460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR72208.1"
                     /translation="MNRIIHISLLISTVSLFLISNTGCVEKQGYYNHGEESIISLICDI
                     TWAGKKTTDENGSVWQGTYKFNKNGTYTRTNIEIDKQGNKKEANIYGQWSFGDPSFSTI
                     YFGGEHYWDIDELTKNKFSFYDRSGKFGDPFMNREYIELTPYQENNTTN"
                     /name="hypothetical protein"
     gene            1607..2377
                     /locus_tag="NXX63_21465"
     CDS             1607..2377
                     /locus_tag="NXX63_21465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR72209.1"
                     /translation="MKKVMFMFISLLTVLGVCSCNDNEVIEPVVSDSVSDMEVLSRFVD
                     VNEITNEYYFNENKKTRALSYVTGSDWQDLEKVSPLSIEKYKNNLQVLNAQVASAISNP
                     NTAYVVFSVNGKTLVKKVKEDANFDFSVFRDVVTETRAVLPSLSINGGSQSTTGVFYDS
                     SRTLKMQVDLNASIQNNYYFFEVLNPNAKPSPDDNITTPESVAFSGTGPLWSNTFTWTS
                     YWDANVPGQGFKWEFKGKGTTPSFGFIANCTFSR"
                     /name="hypothetical protein"
     gene            complement(2447..3148)
                     /locus_tag="NXX63_21470"
     CDS             complement(2447..3148)
                     /locus_tag="NXX63_21470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein 22"
                     /protein_id="UVR72210.1"
                     /translation="METEKAEQLQKDIEQLNRAYTAGTEKITTVQKELSTAQKELNSMK
                     TDIHINEAKEAAAKAGKTVFNAVTSVFNVGEVKRQAKEIETLKKENYNLSFKNQNLEGQ
                     LRTNNIEMRRVQETTDRQIKAEASKLKPITNLFPEMENAQENIEELQTMGIQNKDIRQL
                     LIGKEIYYTGNLYDKDKRKSYQVKNVKIDISKSQNGITTIWLNNIHFKNFLKELWNTLQ
                     KTLDNGNWLHR"
                     /name="coiled-coil domain-containing protein 22"
     gene            complement(3297..3677)
                     /locus_tag="NXX63_21475"
     CDS             complement(3297..3677)
                     /locus_tag="NXX63_21475"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="UVR72203.1"
                     /translation="MAKTCIRVEACNIGSSERHNLRSKELDYIRPELTHRNEQWVECSI
                     AEVHRDITEKYKEATGQGLQKKATPIREGVIVISEETTIQQLQDLAEKLEERFGVHAFQ
                     IYTHKDEKGRSMETELSCAHDL"
                     /name="plasmid recombination protein"
     gene            4083..4331
                     /locus_tag="NXX63_21480"
     CDS             4083..4331
                     /locus_tag="NXX63_21480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="UVR72204.1"
                     /translation="MVTNIIQIGNSKGIILPSEVLKQLRLSLKSAVSISLDGNNIVIKA
                     QPRQGWAEAAKRAHENGDDELLIPDVFEDEKFEDWTW"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            4325..4645
                     /locus_tag="NXX63_21485"
     CDS             4325..4645
                     /locus_tag="NXX63_21485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="UVR72205.1"
                     /translation="MVEQYEVYWVELDPTRGGEMAKTRPCVVVTPSDLNMYLTTVVIVP
                     ITSTIRNYPYRVLCSVAGREGEIATDQIRTVDKSRLKRKIGDLNFSEIKRLREVFQQMF
                     CE"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            4682..5080
                     /locus_tag="NXX63_21490"
     CDS             4682..5080
                     /locus_tag="NXX63_21490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-poly-alpha-D-galacturonosidase"
                     /protein_id="UVR72206.1"
                     /translation="MKPTDYIEWDNLKDIPFFLCQVVEDREKQDLDIYYLGKRVLHDYD
                     HVGHYLRTAVILFRRVKSRTADWVNLRNLWTLRNCVRENYNHGIGMNDLIFGENFDGDN
                     LDTLTPLTKKRFDFLCKRIKELDPYATI"
                     /name="exo-poly-alpha-D-galacturonosidase"
     Typing          2..1050
                     /name="rep_cluster_870"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.81"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2451..3343)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(3346..3677)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 atgtgcaaag atagaacaaa tataaccaac tgcaaagaaa gtccgttaaa aaacggttct
       61 gatagtctta aaaaaaaggc taaagtaaaa atcatatccc ataattttac tcgaaaactt
      121 tcggaaaaga atccggatag taagctgatg aaagcatata tggatgccca tgtgtgttca
      181 aatgtttata ctgtgcaggg aggtaaggcg cattcccatt attgtcatcg ggctttttgt
      241 cctatctgtc agcgtattca aacggcacaa aatgtaaaga agttccttcc ggttatgaag
      301 tatttggctt cggaaggcag agagttttat tttgtgacgc ttaccttgca aaattgtgta
      361 accgatgatg caagggtatt gcgagatttc atgcgtagat gtaaccggat gtgggctgat
      421 ggtataagga cgaaacacaa gtttcgttca cttggtattt cgggggtgat taagaaggag
      481 tgtacctatc atgtagtaaa aaaggatttg ttttcgttcc attatcattt tcatattatt
      541 gttgattcat tggaagctgc aaaatatgta gtagcccaat ggaagaaatt gcatggtagt
      601 acggttgctg atgcccgttt tcagaaatat aagaagatag aggattttga aaatgcagcg
      661 atagaggttt ttaagtatgc ttcaaaggct tctgtctcaa aatcgaaagg tcgtgacggt
      721 aaaaaaaaga ttcagataaa ttataaggct ttggatatga tttatactgc catgcatggg
      781 atgcagcgca tgagtagttt cggacagttc cgcaccatga tcggagatga agcgttgaat
      841 gattttagag atgaggattt ggttcttaat attgagggat tgaatgttga agatggttgt
      901 tatacttggt atatgtctgt gaaagataag gtggctgatt ggtataatat ggaaactggg
      961 gaggcattgt gttgttataa ggtcacccgg aaggatgaac ttttgcagga tatttattta
     1021 gatgaggata tttttcctaa tagtgggtag ttgttagttt gttgtattgt tttcttggta
     1081 tggtgtgagt tctatgtatt ctctattcat gaaaggatcg ccaaattttc cacttctgtc
     1141 atagaaggaa aatttgtttt tagttagctc gtctatgtcc cagtaatgtt ctccaccaaa
     1201 ataaatggta ctgaaacttg gatcaccgaa tgaccattgt ccgtagatat tggcttcttt
     1261 tttatttcct tgtttgtcga tttctatatt tgtacgggta tatgttccgt ttttattgaa
     1321 tttgtatgta ccttgccaga cactgccgtt ttcgtctgtt gtttttttac cagcccatgt
     1381 aatatcacaa attaatgaga ttatgctttc ttctccatgg ttgtagtatc cttgtttttc
     1441 aacacatccg gtgtttgaaa taagaaaaag tgatactgtt gaaataagta aagagatatg
     1501 tattattcta ttcatagtta aatatttaat attcaatgca aaaataggag aaattttata
     1561 tttttgggcg aaacctttat ttattaactt taaaaaagga ggatttatga aaaaagtaat
     1621 gtttatgttc atcagtctac tgactgtttt gggtgtttgt tcgtgtaatg ataatgaagt
     1681 aatagaacct gtggttagtg attctgtatc tgatatggag gtcttatctc gttttgttga
     1741 tgtaaatgag attacaaatg aatactattt caatgagaat aagaaaacga gggcgttgtc
     1801 ttatgtaaca ggttcggatt ggcaggattt agaaaaagtt agtcctttga gtattgagaa
     1861 atataagaat aacctgcagg ttttaaatgc tcaagtggca agtgctattt caaatcctaa
     1921 taccgcttat gttgtttttt ctgtaaatgg aaagactttg gttaaaaagg tgaaggagga
     1981 tgctaatttt gatttttcag tatttaggga tgttgtaacg gaaacgaggg ctgttctccc
     2041 ttctttgagt attaatggag gttctcaaag cactaccgga gttttttacg attccagtcg
     2101 tactttaaag atgcaagttg atttgaatgc ttcaatacag aataattatt atttctttga
     2161 agtattaaat cctaatgcta aaccaagtcc ggatgataat ataacgacac ctgaaagtgt
     2221 tgcttttagt ggaactggac cgttatggag taacactttc acttggactt cgtattggga
     2281 tgcaaatgta cctggtcaag gttttaaatg ggagtttaaa ggaaaaggta ctactccttc
     2341 ttttggtttt atagctaatt gcacctttag tcgttaaaaa atgaacggga tttaatttga
     2401 attgcacttc tcaaaaaaga gaagtgcaat tcaaattagt gtctttctat cggtgtagcc
     2461 agttaccgtt atctaaagtc ttttgtagtg tattccaaag ttctttaaga aagtttttaa
     2521 agtggatgtt atttagccaa attgtagtaa taccattctg acttttggag atatctattt
     2581 tcacgttttt aacttggtat gattttctct tgtctttatc gtaaaggttt ccagtgtagt
     2641 atatttcctt tccaataaga agttgtcgga tatctttatt ttgtattccc atagtttgga
     2701 gttcctctat attttcctgt gcattttcca tttccggaaa aaggttggtt atgggtttta
     2761 gtttggatgc ttctgccttt atctgtctgt cggttgtttc ctgtaccctt cgcatctcga
     2821 tgttgttggt tcttaattgt ccttctaaat tttgattctt gaatgagagg ttataatttt
     2881 cttttttgag ggtttcaatt tctttggctt gtcgttttac ttcccctaca ttaaagacgc
     2941 ttgtaacggc attaaatacg gtttttccgg cttttgcggc agcttctttg gcttcgttga
     3001 tgtgtatgtc ggttttcatg ctgtttagct ctttttgtgc cgtactaagt tccttttgga
     3061 ctgtggtgat tttctcggtt ccggcagtgt atgccctgtt tagttgctcg atgtcttttt
     3121 gaagttgttc agctttctct gtttccattt tgtttttgta ttgcatggcc gacaagtgtt
     3181 tcttgtctga agaaactcca cgttccatgt tgagacactc tgctgttata gtctgcatct
     3241 ccgccatgtc gtgtctgttc agctttacag tttttcctgt atggccgtcc gtccagtcaa
     3301 agatcatgtg cgcatgatag ttcggtttcc atgcttcttc ctttttcatc cttatgggtg
     3361 tagatttgga aggcgtgcac tccaaagcgt tcttccagtt tttcggccaa gtcttgaagc
     3421 tgctggattg tggtttcctc gctgattact attacccctt cccggattgg ggtagctttc
     3481 ttctgtaatc cttgtccggt ggcttccttg tatttttccg ttatgtcccg atgtacttcg
     3541 gcaatggaac attctaccca ctgttcgttg cggtgtgtga gttccggacg gatgtagtcg
     3601 agctccttgc ttcttaagtt gtgacgttcc gagctgccta tattgcaagc ttccactctg
     3661 atacatgtct ttgccatatc tttaggtttt aggtgtgtat gtctgtttag aagatgtggc
     3721 gtgaaacgtc acatcgcacg gggtcgaggg gacacccctc gcacacaacc cctttagggg
     3781 ttatagtgtg ctataacctt ttgcaaaggt aagccttccg gacgggattt gcaagcgctc
     3841 tgccatgtgt tccggatgtg cttgggaaca gaagccggac aaaagggaaa aggacacaag
     3901 gcacaataaa aaatcatcgg tcggggagcg ctgcacggca agggggtgca ccaacactcc
     3961 cgacgagtgc agccattgac agggttctcg gtgaaaatgg aaaaaagttg tttttccttt
     4021 tgttattgtt ataactttac atatatttgt atcgtgataa tcaattataa agttataaca
     4081 ttatggtaac gaatattatt cagattggaa acagcaaggg gataatactt ccttccgaag
     4141 tattgaaaca gttgcgtttg tccttgaaat cggctgtgag catttctttg gacgggaaca
     4201 atattgtgat aaaggcacaa ccacgtcagg gatgggcgga agctgccaaa cgtgcccatg
     4261 agaatgggga tgatgagttg cttatccctg atgtgtttga ggatgaaaag tttgaggatt
     4321 ggacatggta gaacagtatg aagtgtattg ggtagagtta gaccctaccc gtgggggtga
     4381 aatggcaaag acacggccat gtgtagttgt cactccttca gatttgaata tgtaccttac
     4441 aacagtagtc attgttccga taacctcaac gataagaaat tatccttacc gggttctgtg
     4501 ttctgtagcc gggcgtgaag gagagatagc cactgatcaa attcgcacgg tggataaaag
     4561 ccggctgaaa agaaagattg gagatttgaa tttcagtgag ataaaaaggt tacgtgaagt
     4621 atttcagcaa atgttctgtg aataatggaa agtacggaaa gaaaaatgca aataagacat
     4681 tatgaaacca actgactaca tcgaatggga taacctgaag gatattccat tcttcctctg
     4741 tcaagtagtc gaggacagag aaaaacaaga tttggatatt tactatttgg gcaaacgtgt
     4801 cttgcacgat tacgaccatg tggggcacta tttgcggact gccgttatct tgttccgcag
     4861 ggtaaagagc cgcacagcag actgggtaaa cctgcgtaac ctttggacgc tgcgcaactg
     4921 tgtgcgtgag aactacaatc acggcatagg aatgaacgac cttatttttg gggagaattt
     4981 cgatggagat aatctcgata cgcttacacc acttacaaag aaacgctttg attttttgtg
     5041 taagcggatt aaggaacttg acccatatgc aactatttaa cactccattg agtgaaggca
     5101 gggcttgtct ttgtgtgctc cccctttggg ggacgggagg gggataagaa aaaaaatacc
     5161 accggagatg tcggatatcc gtcaattcac tccggtgttt tttttgcaac tctcccgtat
     5221 cgctcggcaa gggttgtctt tgtgtacggc aaagatagtt ttttttccgg aagtatcttt
     5281 gtctgttgtg gatttagtag tgtatttgtg tcagaattgc gcagccgggg gaatgggggc
     5341 gagcccccat actagataaa ctagaaagtt tcgtggtaaa tgctttttga cttgttatat
     5401 gtaggttttt tgaatgatgt ttggcggttt ggaatgaatt acctactttt gggctgagaa
     5461 aaaacgaaag cccgaaggca gtaaccaacg ggctttgtat tttaagaatt agcaatcgtt
     5521 tgtgttcgtt cttggcgggg cggataccaa acgaaagcca catcaaaatt tcat
//
