LOCUS       CP103217                4115 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-70 plasmid unnamed1, complete
            sequence.
ACCESSION   CP103217
VERSION     CP103217.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323516
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 140x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 22:28:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,480
            Genes (coding)                    :: 4,394
            CDSs (with protein)               :: 4,394
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 41 of 86
            Pseudo Genes (incomplete)         :: 50 of 86
            Pseudo Genes (internal stop)      :: 11 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-70"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="1985-10-04"
     gene            join(3365..4115,1..44)
                     /locus_tag="NXX45_22840"
     CDS             join(3365..4115,1..44)
                     /locus_tag="NXX45_22840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UVR58725.1"
                     /translation="MIGKGKSISHGVAALEYDLAKEINGQAVATEIARHELYGCTGAEM
                     VQEMKPYHIDFPNVKNNCLRFEVSPSIEESATFTDADWAELGNDFMQRMGLANHQYIII
                     RHSGTESKKEQAHLHILANRVSLSGELYRDNWIGKKATEAANAIAKERNFVQSQDIGKV
                     NKAEIKEAMDGVLKKMQGFDFTKFKEELGKRGFKVREARASTGKLNGYYVTARSGTEYK
                     ASEIGKGYTLAHIERTQSKLKCNSMNISHGNKLTPGSGSFQR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1..453
                     /locus_tag="NXX45_22845"
     CDS             1..453
                     /locus_tag="NXX45_22845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR58726.1"
                     /translation="METNSHPEAAASNVKQGQYSPRKRKDTTPRVNPQLAVELVYQELK
                     RVEVYTKRIEDATARKVQIDGKSLESAENRLKNVLADFERQGYRMKNGGYVDKRISFYS
                     ILCAVISLLFACFMCYLWTDAAKDRDNYKQYYEYYQEQAREQKGNK"
                     /name="hypothetical protein"
     gene            473..703
                     /locus_tag="NXX45_22850"
     CDS             473..703
                     /locus_tag="NXX45_22850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="charged multivesicular body protein 2b-B"
                     /protein_id="UVR58727.1"
                     /translation="MGTTEHEEPRFFFILNKGAKSGGEITHAVLNGSIVSKPAGWDAFH
                     GLALAREKLSSEEIQQQMKELGVEMEIVPLI"
                     /name="charged multivesicular body protein 2b-B"
     gene            complement(740..1765)
                     /locus_tag="NXX45_22855"
     CDS             complement(740..1765)
                     /locus_tag="NXX45_22855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UVR58728.1"
                     /translation="MKKKLPITKNKDVVVSWVYTWSKQQDMSIHEQRIVLRILEACQAE
                     LKGVKLKDYAGTKRKFEHGLWDVDAQMHVSDVIFSGRDYNEIIAALDSLAGRFFTYEDD
                     EEWWKCGFISNPKYKKRTGIITFRVSNDLWDVFTKFAKGYREFELNKALALPTGYSLRF
                     YMLMSGQVYPLDISLENLKDRLGIPADKYKDKNGKDRIDHFEERVLKPAKAALDESCPY
                     TFNYVKVRENPNNKRSKVTGFRFYPVYQPQFRDEELEGKELQAKVTARYQIDSHVYEYL
                     RYSCGFTSEEINRNKETFITAQEKITDLIGELALLNGKSREKNNPKGWIINALKGKIKD
                     K"
                     /name="replication initiation protein"
     gene            2178..2396
                     /locus_tag="NXX45_22860"
     CDS             2178..2396
                     /locus_tag="NXX45_22860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR58722.1"
                     /translation="MEALSVREYRNNLAASFTKADNGEQVLIRRKNEIYALVKVGREDL
                     MITPELQARIDKTLKSSEDIDAYFDSL"
                     /name="hypothetical protein"
     gene            2393..2668
                     /locus_tag="NXX45_22865"
     CDS             2393..2668
                     /locus_tag="NXX45_22865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004519 - endonuclease activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family toxin"
                     /protein_id="UVR58723.1"
                     /translation="MMYTIRVSDGVDKVIAKWKKSNPNLFKKYKKIYKELLEHPKTGLG
                     HPEALRGGGDITWSRHITAHDRIIYDIYEEVVEVYILEVEGHYNDK"
                     /name="type II toxin-antitoxin system YoeB family toxin"
     gene            2679..2876
                     /locus_tag="NXX45_22870"
     CDS             2679..2876
                     /locus_tag="NXX45_22870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR58724.1"
                     /translation="MVEYCVYWLENGEPMHEVFSSLAAAEMYSCAIRGKENIEWVEVSE
                     EETIDLDELEDMFPDDFCGV"
                     /name="hypothetical protein"
     Typing          3366..4108
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="81.452"
                     /coverage="94.0"
                     /evalue="4.18e-139"
ORIGIN
        1 atggaaacaa actcacaccc ggaagcggca gcttccaacg ttaagcaggg acagtacagc
       61 ccccggaaaa gaaaagatac aacgccaagg gtaaacccgc agcttgcggt agaactggtg
      121 tatcaagaac taaagcgtgt tgaagtctat acgaagcgca tagaggacgc aacagcccga
      181 aaggtacaaa tagacgggaa aagccttgaa agtgccgaaa atcgcctaaa aaacgtgttg
      241 gcggactttg aacggcaggg gtacagaatg aaaaacggcg gttatgtgga caaacgaatt
      301 tcgttctact caatcctttg tgctgttatc tccctattgt tcgcttgttt catgtgctat
      361 ctttggacgg atgcagccaa agaccgggac aactacaagc agtattatga atactaccaa
      421 gagcaggcaa gggaacaaaa ggggaataaa taaacatcta actttacgaa ttatgggaac
      481 aacggaacac gaagaacccc ggttcttctt catactgaac aagggtgcga agtccggcgg
      541 cgaaatcacc catgccgtcc taaacggtag tatcgtttcg aagccggcag gctgggacgc
      601 ctttcatggg cttgcactgg cgagagagaa actaagcagc gaggaaatac aacagcagat
      661 gaaagaactt ggtgtagaaa tggaaatcgt tcctctgatt tgacaagatt acccggagtg
      721 tttcagccgg gtaatcttgt tacttatcct tgattttccc tttaagggcg tttataatcc
      781 accctttcgg attgttcttt tctcgtgatt ttccgtttag gagagccagt tctccgataa
      841 ggtcggttat cttttcttgt gccgttatga atgtctcttt gttccggttt atctcttccg
      901 atgtgaagcc gcaggaataa cggaggtact cgtacacatg gctgtctatc tgatatcgtg
      961 ctgtaacctt tgcttgcaat tctttccctt ccagttcttc atctctgaac tgtggttgat
     1021 agaccgggta gaacctaaac ccggtcacct tgctacgttt gttgtttggg ttttcacgca
     1081 ctttcacgta gttgaaagtg tatgggcagc tctcatcaag cgcagccttt gctggtttca
     1141 aaactctttc ctcaaaatga tctattctgt cttttccgtt cttgtccttg tatttgtccg
     1201 caggtatgcc aagacggtct ttcaagtttt caagggatat atccaaaggg tacacctgcc
     1261 cactcattaa catgtaaaac ctaagggaat acccagtagg tagcgcaaga gccttgttta
     1321 actcaaattc tctgtacccc ttggcgaact tggtaaacac atcccaaagg tcgttagaca
     1381 cacgaaaggt tataatgcca gtgcgttttt tatattttgg gttagatata aatccgcact
     1441 tccaccattc ctcgtcatct tcgtaagtaa aaaaacgtcc tgccaaagaa tcgagtgcgg
     1501 ctatgatttc attgtaatca cgtccggaaa aaataacgtc agatacatgc atttgtgcat
     1561 caacatccca aagtccatgc tcaaacttgc gtttcgtgcc tgcataatct ttcagtttta
     1621 ctccctttag ttcagcttgg cacgcttcca gtatgcgaag aactattctt tgttcgtgta
     1681 tggacatgtc ttgctgtttt gaccatgtat atacccatga aacaacaaca tctttattct
     1741 tggtaatagg tagtttcttt ttcataaacg attgatttgc tgcaaatata cacaatcccc
     1801 acattaaaac aaaaaaagag gggtaaaact cccaaagtgt ggggactaat ttacttttaa
     1861 actcccaaag tgtggggact aaactcccaa agtgtgggga aacacatgtt ctaatccctt
     1921 tatttatggg cgtttgcgaa gaagtgatag agcatattac agacgatgct tataacagac
     1981 aaggggaatt tatagagcta agtggttgat ttactgtcgg aaaaagaaaa acgataggga
     2041 aaaagagcaa agcaaaacca accaacgggc ggtaggattg gaaacgggtt tttatggcgt
     2101 gtacacgttt gtgtacaaaa tgtttgtatt ttattgaaat atagctatct ttgtgctgaa
     2161 tttaaaagca aacagatatg gaagcattat cagtaagaga ataccgtaac aacctcgcag
     2221 cgtctttcac caaagctgac aatggcgagc aggtactaat tcgcaggaaa aacgagattt
     2281 acgctttggt aaaagttggt cgtgaagatt tgatgataac cccggagctg caagcaagga
     2341 ttgacaagac cctcaaaagc agtgaggata ttgacgctta tttcgatagt ctatgatgta
     2401 cacaattagg gtttccgacg gggtggataa ggtgattgcc aaatggaaga aatcaaaccc
     2461 caatttgttc aagaagtata agaaaatcta caaagaattg ttagagcacc ctaagactgg
     2521 gcttgggcat cccgaagcgt taaggggcgg tggggatatt acatggtctc gacacattac
     2581 cgcccatgac cggataatct atgacattta cgaggaagtg gtagaggtct acatcttgga
     2641 ggtagagggg cattacaacg ataaatgata aaactgttat ggtcgaatat tgtgtttact
     2701 ggttagagaa cggcgagcct atgcacgagg tgttctcttc tcttgccgcc gccgagatgt
     2761 actcatgtgc gataagaggg aaagaaaaca ttgaatgggt ggaggtgtcc gaagaagaaa
     2821 ccattgattt ggacgaactg gaagacatgt tcccggatga cttctgcggc gtgtaatccc
     2881 cttgggtaga aatggggtag aatttgttag gctgtggggg attttctaac acgccggggc
     2941 tttttgctgg gcggttggcg tggatgtatt cccatgcggc atgtgtatat atagcaagaa
     3001 gtgtccttgt cggacaattc ttgcttttct cgctttgctc aaaaagattt taagattacc
     3061 tttgtggcat ggaactaaga cggaacgaaa agattacatt ccggtgtacc gaacttgaaa
     3121 aggacgcact tgcggagcag gcagcccggt gcagtctaag cgtatcggaa tactgccgga
     3181 gtttgtccct tggggggcgc ccaagggaga ggtacaccga ggaggaacgg cagcttctcc
     3241 gggacatagc ccagttgaaa ggaacgctcc aacggctgaa caactacttc ggtggtcgcc
     3301 agtaccggga agtgttcgag gagaaccggg cactaattac agagctcaaa aaaatactct
     3361 cacgatgata gggaaaggga aaagcatatc acatggtgtg gcggcattgg agtacgacct
     3421 cgccaaagag ataaacgggc aggcagtagc caccgagata gcccggcatg aactttatgg
     3481 gtgtactggt gcggaaatgg tgcaggaaat gaaaccgtat cacattgatt ttccgaacgt
     3541 aaaaaacaac tgcctacgtt tcgaggtcag cccctcgata gaggaaagcg ccacctttac
     3601 ggatgcggac tgggcggaac ttggtaacga cttcatgcag cgcatggggt tggcgaacca
     3661 ccagtacatc atcatccggc acagcggtac ggagagcaag aaagagcaag cccacctgca
     3721 catactggca aaccgggttt ccctttccgg ggaactgtac cgggacaact ggatagggaa
     3781 aaaggcaacg gaagccgcca atgccatagc caaggaacgg aactttgtac agtcgcagga
     3841 cattggcaaa gtcaacaaag cggaaatcaa ggaagccatg gacggcgtat tgaagaagat
     3901 gcagggcttt gactttacca aattcaagga agaacttggt aagagaggtt ttaaagtccg
     3961 ggaagccagg gcaagcaccg ggaaacttaa cggctactat gtcacagccc gaagcggtac
     4021 ggagtataag gcaagcgaga tagggaaagg ctacactctc gcccatatcg agcggacaca
     4081 aagcaaactg aagtgcaact caatgaacat atctc
//
