LOCUS       CP103205                2746 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-539 plasmid unnamed2, complete
            sequence.
ACCESSION   CP103205
VERSION     CP103205.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323434
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 193x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 21:42:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,410
            CDSs (total)                      :: 4,317
            Genes (coding)                    :: 4,036
            CDSs (with protein)               :: 4,036
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 281
            CDSs (without protein)            :: 281
            Pseudo Genes (ambiguous residues) :: 0 of 281
            Pseudo Genes (frameshifted)       :: 247 of 281
            Pseudo Genes (incomplete)         :: 43 of 281
            Pseudo Genes (internal stop)      :: 18 of 281
            Pseudo Genes (multiple problems)  :: 22 of 281
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-539"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="2009-07-02"
     gene            1..1104
                     /locus_tag="NXV70_22045"
     CDS             1..1104
                     /locus_tag="NXV70_22045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="UVR62764.1"
                     /translation="MGATSIHVQAVKPGSEIHNFREKELDYVRPELSHLNESWVGDSIS
                     HRLESAKQRYLDTVGQKMQAKAAPIREGVIVIKQETTMQELQQFATVCKERFGIEAFQI
                     HIHKDEGYMNAKQWTPNLHAHVVFDWTQPNGKSVRLSRDDMAELQTIASETLGMERGVS
                     SDRKHLSAMQYKTECAKEQLQELSNDISSALDKHKDVQNQLLQLQKELRSIETKKNVQK
                     LISKASEKFYGLIGKTVNDREKDALKAKVKALEGENEQLSDRLGKAILEKEQNGTKAFK
                     AENDKEYYRQQMDNARTTSNRLRTENQELKTETKELKKELGKMKDLFNSEQLEALRHHF
                     PNISKAMEEGKDLLKQITRSRGFGMGM"
                     /name="mobilization protein"
     gene            1546..2136
                     /locus_tag="NXV70_22050"
     CDS             1546..2136
                     /locus_tag="NXV70_22050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="UVR62763.1"
                     /translation="MENKKAVKLTDFQKNEENPFMKQAIEGIENHVVKKYKSNSGGDKR
                     AVVALADTETGEVFKTSFIRQIEVDEEQFTKLYLSNFAAFFDLSQAAIRVFGYFMTCMK
                     PKNDLIIFNRKKCLEYTKYKTDKAVYKGLAELVKAEIIARGPADNLWFINPLIVFNGDR
                     VTFAKTYVRKKTLAAQKKEEAEKRQLSLGFDEQ"
                     /name="replication/maintenance protein RepL"
     Typing          1514..2126
                     /name="rep_cluster_1868"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.701"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..1101
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.275"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgggagcaa caagtattca tgtacaagca gtgaagccgg ggagtgagat tcacaacttt
       61 agggaaaaag agttggacta tgttcgtccc gaacttagtc atttgaatga aagctgggtt
      121 ggagatagca tctcccatcg gctggaaagt gcgaaacaaa gatatctcga tacggttggg
      181 cagaagatgc aggctaaagc cgcacccata cgagagggag taatagtaat caaacaagaa
      241 accaccatgc aggaactcca gcagtttgcc acggtctgca aagaacgttt cggtatcgaa
      301 gcatttcaaa tccatataca caaggacgaa ggatacatga acgcaaagca atggacacct
      361 aaccttcatg cccatgtagt tttcgattgg acgcagccga atgggaaaag tgtgcgttta
      421 tcgcgtgatg acatggcaga actccaaacc atagcatctg aaacactggg catggaacgt
      481 ggcgtttctt ctgaccgcaa acacttatcg gctatgcagt acaagaccga atgtgcgaaa
      541 gaacagcttc aggaactatc aaacgatata tccagtgcat tagacaaaca caaggacgta
      601 caaaaccagc ttcttcagct tcagaaagaa ctacgttcca ttgaaacaaa gaagaacgtc
      661 caaaaactca tttctaaggc ttcagagaag ttttacggct tgattggaaa aacagtcaac
      721 gatagggaaa aagatgcctt aaaagccaaa gtaaaggctt tagaggggga aaatgaacag
      781 ctatccgata gactgggaaa ggctatactt gaaaaagagc aaaacggcac aaaggcattc
      841 aaagccgaga atgacaagga gtattataga caacaaatgg acaacgcaag gacaaccagt
      901 aaccgattaa ggacggagaa ccaagaacta aagaccgaaa ccaaggaatt gaaaaaagaa
      961 cttggtaaga tgaaagactt gttcaattcc gaacaactgg aggctttaag gcatcatttc
     1021 ccaaacatat caaaagctat ggaagagggg aaagacctac tcaagcaaat caccagaagc
     1081 agaggtttcg gtatgggtat gtaacccccc ccccgccccc aaaaaggggg agcgaccaaa
     1141 cggcagcctc tctcaatgga gtgttacgtc gttcggattc ggaagaaatt ttgcagggtt
     1201 cggaaaacgg ttgtgtgctt attctaaaaa aagtttatgg tgtggcgctc gtcgggacgg
     1261 gtggtcccgc cccttgccgc tgggcgctcc ccgaccgatg atttttaagt gactgatttt
     1321 gtgctgtttt gggggtatat taagaataga agaaatagaa taagttaagt acttgataca
     1381 caatataggg cattttccat attggaaatt ctcattttcc aatctagaaa atactgattt
     1441 cctaatataa tactaaacag gaaaataata tttcccttaa tattgttttt atggaaaata
     1501 atagtttact ttgtggagaa taatatttcc caaaaacaca tcaaaatgga aaataaaaaa
     1561 gcagttaagt taaccgattt tcaaaagaac gaagaaaatc cttttatgaa acaagctata
     1621 gaaggtattg aaaatcatgt tgttaaaaag tataagagta atagtggtgg cgataagaga
     1681 gccgtagtag ctttagccga cactgaaact ggagaagtgt ttaagacttc gtttatccgt
     1741 caaatagaag tagatgaaga acaattcact aaattgtatc tttctaactt tgctgcattc
     1801 tttgacctat cacaagcagc tattcgggtt tttggttact ttatgacctg catgaaaccc
     1861 aaaaatgatt taatcatctt caatagaaaa aaatgcctag aatataccaa atacaaaaca
     1921 gacaaagccg tttataaagg acttgcagaa cttgtaaaag ctgaaatcat agcccgagga
     1981 ccagccgata atctttggtt tattaatcct ctgatagtat tcaatggtga ccgagtgaca
     2041 tttgctaaaa catacgttcg gaaaaagact ttagctgccc aaaagaaaga agaagcagag
     2101 aaacgacaat tatcacttgg ctttgatgaa cagtaacact ccattgagtg aagctgccgt
     2161 ttggtcgctc cccctttggg ggcggggggg gggatagata aagttcctct atgtaaagtt
     2221 ataatggggg caaggttcgc taaccttacc ccgattgttt atgcttccca gcattcaatc
     2281 tatccccgtt tatgggtttc tctttcaccc tacaaagata acctcatggg ggaaaaatgt
     2341 ccaagaatat ggggtaaaaa ctatcaagtc ggtagaaaat aagtatcttt gaagtacatt
     2401 tttagtagag gtactggtat gcctggacga agcagatagg caaaaataaa gccgctggag
     2461 atgtcggatg tccgtcaatt aactccagcg gcttgtattt tggtaactct cccgtatcgg
     2521 tcggctaccg tttggtcagc aacaaaggta gtactttatt gtcgcaaatc caaatgtttt
     2581 cccctttatt tgtgcattta cagaacattt gctaacttcg cacctatgca gtatggtgat
     2641 agcacactag caccctaaag ggtgcgtgtg taagggacaa gcccccttaa ccccctgtca
     2701 gccggctgtc accggctgtt atcgattgtc acaaaaatgt taactt
//
