LOCUS       CP103204                5666 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-539 plasmid unnamed1, complete
            sequence.
ACCESSION   CP103204
VERSION     CP103204.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323434
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 193x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 21:42:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,410
            CDSs (total)                      :: 4,317
            Genes (coding)                    :: 4,036
            CDSs (with protein)               :: 4,036
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 281
            CDSs (without protein)            :: 281
            Pseudo Genes (ambiguous residues) :: 0 of 281
            Pseudo Genes (frameshifted)       :: 247 of 281
            Pseudo Genes (incomplete)         :: 43 of 281
            Pseudo Genes (internal stop)      :: 18 of 281
            Pseudo Genes (multiple problems)  :: 22 of 281
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-539"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="2009-07-02"
     gene            1..1251
                     /locus_tag="NXV70_22005"
     CDS             1..1251
                     /locus_tag="NXV70_22005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR62758.1"
                     /translation="MAKTCIRVEACNIGSSERHNLRSKELDYIRPELTHRNEQWVECSI
                     AEVHRDITEKYKEATGQGLQKKATPIREGVIVISEETTIQQLQDLAEKLEERFGVHAFQ
                     IYTHKDEGANVWDGKEEAWKPNYHAHMIFDWTDGHTGKTVKLNRHDMAEMQTITAECLN
                     MERGVSSDKKHLSAMQYKNKMETEKAEQLQKDIEQLNRAYTAGTEKITTVQKELSTAQK
                     ELNSMKTDIHINEAKEAAAKAGKTVFNAVTSVFNVGEVKRQAKEIETLKKENYNLSFKN
                     QNLEGQLRTNNIEMRRVQETTDRQIKAEASKLKPITNLFPEMENAQENIEELQTMGIQN
                     KDIRQLLIGKEIYYTGNLYDKDKRKSYQVKNVKIDISKSQNGITTIWLNNIHFKNFLKE
                     LWNTLQKTLDNGNWLHR"
                     /name="hypothetical protein"
     gene            complement(1321..2091)
                     /locus_tag="NXV70_22010"
     CDS             complement(1321..2091)
                     /locus_tag="NXV70_22010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR62759.1"
                     /translation="MKKVMFMFISLLTVLGVCSCNDNEVIEPVVSDSVSDMEVLSRFVD
                     VNEITNEYYFNENKKTRALSYVTGSDWQDLEKVSPLSIEKYKNNLQVLNAQVASAISNP
                     NTAYVVFSVNGKTLVKKVKEDANFDFSVFRDVVTETRAVLPSLSINGGSQSTTGVFYDS
                     SRTLKMQVDLNASIQNNYYFFEVLNPNAKPSPDDNITTPESVAFSGTGPLWSNTFTWTS
                     YWDANVPGQGFKWEFKGKGTTPSFGFIANCTFSR"
                     /name="hypothetical protein"
     gene            2183..2644
                     /locus_tag="NXV70_22015"
     CDS             2183..2644
                     /locus_tag="NXV70_22015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR62760.1"
                     /translation="MNRIIHISLLISTVSLFLISNTGCVEKQGYYNHGEESIISLICDI
                     TWAGKKTTDENGSVWQGTYKFNKNGTYTRTNIEIDKQGNKKEANIYGQWSFGDPSFSTI
                     YFGGEHYWDIDELTKNKFSFYDRSGKFGDPFMNREYIELTPYQENNTTN"
                     /name="hypothetical protein"
     gene            complement(2648..3232)
                     /locus_tag="NXV70_22020"
     CDS             complement(2648..3232)
                     /locus_tag="NXV70_22020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR62761.1"
                     /translation="MIKKECTYHVVKKDLFSFHYHFHIIVDSLEAAKYVVAQWKKLHGS
                     TVADARFQKYKKIEDFENAAIEVFKYASKASVSKSKGRDGKKKIQINYKALDMIYTAMH
                     GMQRMSSFGQFRTMIGDEALNDFRDEDLVLNIEGLNVEDGCYTWYMSVKDKVADWYNME
                     TGEALCCYKVTRKDELLQDIYLDEDIFPNSG"
                     /name="hypothetical protein"
     gene            complement(3229..3621)
                     /locus_tag="NXV70_22025"
     CDS             complement(3229..3621)
                     /locus_tag="NXV70_22025"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="UVR62755.1"
                     /translation="MKAYMDAHVCSNVYTVQGGKAHSHYCHRAFCPICQRIQTAQNVKK
                     FLPVMKYLASEGREFYFVTLTLQNCVTDDARVLRDFMRRCNRMWADGIRTKHKFRSLGI
                     SGVIKKECTYHVVKKDLFSFHYHFHG"
                     /name="protein rep"
     gene            complement(4263..4661)
                     /locus_tag="NXV70_22030"
     CDS             complement(4263..4661)
                     /locus_tag="NXV70_22030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-poly-alpha-D-galacturonosidase"
                     /protein_id="UVR62756.1"
                     /translation="MKPTDYIEWDNLKDIPFFLCQVVEDREKQDLDIYYLGKRVLHDYD
                     HVGHYLRTAVILFRRVKSRTADWVNLRNLWTLRNCVRENYNHGIGMNDLIFGENFDGDN
                     LDTLTPLTKKRFDFLCKRIKELDPYATI"
                     /name="exo-poly-alpha-D-galacturonosidase"
     gene            complement(4698..5018)
                     /locus_tag="NXV70_22035"
     CDS             complement(4698..5018)
                     /locus_tag="NXV70_22035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="UVR62762.1"
                     /translation="MVEQYEVYWVELDPTRGGEMAKTRPCVVVTPSDLNMYLTTVVIVP
                     ITSTIRNYPYRVLCSVAGREGEIATDQIRTVDKSRLKRKIGDLNFSEIKRLREVFQQMF
                     CE"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(5012..5260)
                     /locus_tag="NXV70_22040"
     CDS             complement(5012..5260)
                     /locus_tag="NXV70_22040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="UVR62757.1"
                     /translation="MVTNIIQIGNSKGIILPSEVLKQLRLSLKSAVSISLDGNNIVIKA
                     QPRQGWAEAAKRAHENGDDELLIPDVFEDEKFEDWTW"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     Typing          complement(2649..3234)
                     /name="rep_cluster_870"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3236..3768)
                     /name="rep_cluster_870"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..1248
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaaga catgtatcag agtggaagcc tgcaatatag gcagctcgga acgtcacaac
       61 ttaagaagca aggagctcga ctacatccgt ccggaactca cacaccgcaa cgaacagtgg
      121 gtagaatgtt ccattgccga agtacatcgg gacataacgg aaaaatacaa ggaagccacc
      181 ggacaaggat tacagaagaa agctacccca atccgggaag gggtaatagt aatcagcgag
      241 gaaaccacaa tccagcagct tcaagacttg gccgaaaaac tggaagaacg ctttggagtg
      301 cacgccttcc aaatctacac ccataaggat gaaggcgcaa acgtatggga cggaaaggaa
      361 gaagcatgga aaccgaacta tcatgcgcac atgatctttg actggacgga cggccataca
      421 ggaaaaactg taaagctgaa cagacacgac atggcggaga tgcagactat aacagcagag
      481 tgtctcaaca tggaacgtgg agtttcttca gacaagaaac acttgtcggc catgcaatac
      541 aaaaacaaaa tggaaacaga gaaagctgaa caacttcaaa aagacatcga gcaactaaac
      601 agggcataca ctgccggaac cgagaaaatc accacagtcc aaaaggaact tagtacggca
      661 caaaaagagc taaacagcat gaaaaccgac atacacatca acgaagccaa agaagctgcc
      721 gcaaaagccg gaaaaaccgt atttaatgcc gttacaagcg tctttaatgt aggggaagta
      781 aaacgacaag ccaaagaaat tgaaaccctc aaaaaagaaa attataacct ctcattcaag
      841 aatcaaaatt tagaaggaca attaagaacc aacaacatcg agatgcgaag ggtacaggaa
      901 acaaccgaca gacagataaa ggcagaagca tccaaactaa aacccataac caaccttttt
      961 ccggaaatgg aaaatgcaca ggaaaatata gaggaactcc aaactatggg aatacaaaat
     1021 aaagatatcc gacaacttct tattggaaag gaaatatact acactggaaa cctttacgat
     1081 aaagacaaga gaaaatcata ccaagttaaa aacgtgaaaa tagatatctc caaaagtcag
     1141 aatggtatta ctacaatttg gctaaataac atccacttta aaaactttct taaagaactt
     1201 tggaatacac tacaaaagac tttagataac ggtaactggc tacaccgata gaaagacact
     1261 aatttgaatt gcacttctct tttttgagaa gtgcaattca aattaaatcc cgttcatttt
     1321 ttaacgacta aaggtgcaat tagctataaa accaaaagaa ggagtagtac cttttccttt
     1381 aaactcccat ttaaaacctt gaccaggtac atttgcatcc caatacgaag tccaagtgaa
     1441 agtgttactc cataacggtc cagttccact aaaagcaaca ctttcaggtg tcgttatatt
     1501 atcatccgga cttggtttag cattaggatt taatacttca aagaaataat aattattctg
     1561 tattgaagca ttcaaatcaa cttgcatctt taaagtacga ctggaatcgt aaaaaactcc
     1621 ggtagtgctt tgagaacctc cattaatact caaagaaggg agaacagccc tcgtttccgt
     1681 tacaacatcc ctaaatactg aaaaatcaaa attagcatcc tccttcacct ttttaaccaa
     1741 agtctttcca tttacagaaa aaacaacata agcggtatta ggatttgaaa tagcacttgc
     1801 cacttgagca tttaaaacct gcaggttatt cttatatttc tcaatactca aaggactaac
     1861 tttttctaaa tcctgccaat ccgaacctgt tacataagac aacgccctcg ttttcttatt
     1921 ctcattgaaa tagtattcat ttgtaatctc atttacatca acaaaacgag ataagacctc
     1981 catatcagat acagaatcac taaccacagg ttctattact tcattatcat tacacgaaca
     2041 aacacccaaa acagtcagta gactgatgaa cataaacatt acttttttca taaatcctcc
     2101 ttttttaaag ttaataaata aaggtttcgc ccaaaaatat aaaatttctc ctatttttgc
     2161 attgaatatt aaatatttaa ctatgaatag aataatacat atctctttac ttatttcaac
     2221 agtatcactt tttcttattt caaacaccgg atgtgttgaa aaacaaggat actacaacca
     2281 tggagaagaa agcataatct cattaatttg tgatattaca tgggctggta aaaaaacaac
     2341 agacgaaaac ggcagtgtct ggcaaggtac atacaaattc aataaaaacg gaacatatac
     2401 ccgtacaaat atagaaatcg acaaacaagg aaataaaaaa gaagccaata tctacggaca
     2461 atggtcattc ggtgatccaa gtttcagtac catttatttt ggtggagaac attactggga
     2521 catagacgag ctaactaaaa acaaattttc cttctatgac agaagtggaa aatttggcga
     2581 tcctttcatg aatagagaat acatagaact cacaccatac caagaaaaca atacaacaaa
     2641 ctaacaacta cccactatta ggaaaaatat cctcatctaa ataaatatcc tgcaaaagtt
     2701 catccttccg ggtgacctta taacaacaca atgcctcccc agtttccata ttataccaat
     2761 cagccacctt atctttcaca gacatatacc aagtataaca accatcttca acattcaatc
     2821 cctcaatatt aagaaccaaa tcctcatctc taaaatcatt caacgcttca tctccgatca
     2881 tggtgcggaa ctgtccgaaa ctactcatgc gctgcatccc atgcatggca gtataaatca
     2941 tatccaaagc cttataattt atctgaatct tttttttacc gtcacgacct ttcgattttg
     3001 agacagaagc ctttgaagca tacttaaaaa cctctatcgc tgcattttca aaatcctcta
     3061 tcttcttata tttctgaaaa cgggcatcag caaccgtact accatgcaat ttcttccatt
     3121 gggctactac atattttgca gcttccaatg aatcaacaat aatatgaaaa tgataatgga
     3181 acgaaaacaa atcctttttt actacatgat aggtacactc cttcttaatc acccatgaaa
     3241 atgataatgg aacgaaaaca aatccttttt tactacatga taggtacact ccttcttaat
     3301 cacccccgaa ataccaagtg aacgaaactt gtgtttcgtc cttataccat cagcccacat
     3361 ccggttacat ctacgcatga aatctcgcaa tacccttgca tcatcggtta cacaattttg
     3421 caaggtaagc gtcacaaaat aaaactctct gccttccgaa gccaaatact tcataaccgg
     3481 aaggaacttc tttacatttt gtgccgtttg aatacgctga cagataggac aaaaagcccg
     3541 atgacaataa tgggaatgcg ccttacctcc ctgcacagta taaacatttg aacacacatg
     3601 ggcatccata tatgctttca tcagcttact atccggattc ttttccgaaa gttttcgagt
     3661 aaaattatgg gatatgattt ttactttagc ctttttttta agactatcag aaccgttttt
     3721 taacggactt tctttgcagt tggttatatt tgttctatct ttgcacatat gaaattttga
     3781 tgtggctttc gtttggtatc cgccccgcca agaacgaaca caaacgattg ctaattctta
     3841 aaatacaaag cccgttggtt actgccttcg ggctttcgtt tttttcaggc ccaaaagtag
     3901 gtaattcatt ccaaaccgcc aaacatcatt caaaaaacct acatataaca agtcaaaaag
     3961 catttaccac gaaactttct agtttatcta gtatgggggc tcgcccccat tcccccggct
     4021 gcgcaattct gacacaaata cactactaaa tccacaacag acaaagatac ttccggaaaa
     4081 aaaactatct ttgccgtaca caaagacaac ccttgccgag cgatacggga gagttgcaaa
     4141 aaaaacaccg gagtgaattg acggatatcc gacatctccg gtggtatttt ttcctttatc
     4201 cccctcccgt cccccaaagg gggagcacac aaagacaagc cctgccttca ctcaatggag
     4261 tgttaaatag ttgcatatgg gtcaagttcc ttaatccgct tacacaaaaa atcaaagcgt
     4321 ttctttgtaa gtggtgtaag cgtatcgaga ttatctccat cgaaattctc cccaaaaata
     4381 aggtcgttca ttcctatgcc gtgattgtag ttctcacgca cacagttgcg cagcgtccaa
     4441 aggttacgca ggtttaccca gtctgctgtg cggctcttta ccctgcggaa caagataacg
     4501 gcagtccgca aatagtgccc cacatggtcg taatcgtgca agacacgttt gcccaaatag
     4561 taaatatcca aatcttgttt ttctctgtcc tcgactactt gacagaggaa gaatggaata
     4621 tccttcaggt tatcccattc gatgtagtca gttggtttca taatgtctta tttgcatttt
     4681 tctttccgta ctttccatta ttcacagaac atttgctgaa atacttcacg taaccttttt
     4741 atctcactga aattcaaatc tccaatcttt cttttcagcc ggcttttatc caccgtgcga
     4801 atttgatcag tggctatctc tccttcacgc ccggctacag aacacagaac ccggtaagga
     4861 taatttctta tcgttgaggt tatcggaaca atgactactg ttgtaaggta catattcaaa
     4921 tctgaaggag tgacaactac acatggccgt gtctttgcca tttcaccccc acgggtaggg
     4981 tctaactcta cccaatacac ttcatactgt tctaccatgt ccaatcctca aacttttcat
     5041 cctcaaacac atcagggata agcaactcat catccccatt ctcatgggca cgtttggcag
     5101 cttccgccca tccctgacgt ggttgtgcct ttatcacaat attgttcccg tccaaagaaa
     5161 tgctcacagc cgatttcaag gacaaacgca actgtttcaa tacttcggaa ggaagtatta
     5221 tccccttgct gtttccaatc tgaataatat tcgttaccat aatgttataa ctttataatt
     5281 gattatcacg atacaaatat atgtaaagtt ataacaataa caaaaggaaa aacaactttt
     5341 ttccattttc accgagaacc ctgtcaatgg ctgcactcgt cgggagtgtt ggtgcacccc
     5401 cttgccgtgc agcgctcccc gaccgatgat tttttattgt gccttgtgtc cttttccctt
     5461 ttgtccggct tctgttccca agcacatccg gaacacatgg cagagcgctt gcaaatcccg
     5521 tccggaaggc ttacctttgc aaaaggttat agcacactat aacccctaaa ggggttgtgt
     5581 gcgaggggtg tcccctcgac ccccgtgcga tgtgacgttt cacgccacat cttctaaaca
     5641 gacatacaca cctaaaacct aaagat
//
