LOCUS       CP103202                5599 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-642 plasmid unnamed3, complete
            sequence.
ACCESSION   CP103202
VERSION     CP103202.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323513
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 189x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 21:28:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,759
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 289
            CDSs (without protein)            :: 289
            Pseudo Genes (ambiguous residues) :: 0 of 289
            Pseudo Genes (frameshifted)       :: 255 of 289
            Pseudo Genes (incomplete)         :: 41 of 289
            Pseudo Genes (internal stop)      :: 12 of 289
            Pseudo Genes (multiple problems)  :: 18 of 289
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-642"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="2018-12-14"
     gene            1..771
                     /locus_tag="NXV98_23765"
     CDS             1..771
                     /locus_tag="NXV98_23765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR50256.1"
                     /translation="MKKVMFMFISLLTVLGVCSCNDNEVIEPVVSDSVSDMEVLSRFVD
                     VNEITNEYYFNENKKTRALSYVTGSDWQDLEKVSPLSIEKYKNNLQVLNAQVASAISNP
                     NTAYVVFSVNGKTLVKKVKEDANFDFSVFRDVVTETRAVLPSLSINGGSQSTTGVFYDS
                     SRTLKMQVDLNASIQNNYYFFEVLNPNAKPSPDDNITTPESVAFSGTGPLWSNTFTWTS
                     YWDANVPGQGFKWEFKGKGTTPSFGFIANCTFSR"
                     /name="hypothetical protein"
     gene            complement(841..2091)
                     /locus_tag="NXV98_23770"
     CDS             complement(841..2091)
                     /locus_tag="NXV98_23770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR50257.1"
                     /translation="MAKTCIRVEACNIGSSERHNLRSKELDYIRPELTHRNEQWVECSI
                     AEVHRDITEKYKEATGQGLQKKATPIREGVIVISEETTIQQLQDLAEKLEERFGVHAFQ
                     IYTHKDEGANVWDGKEEAWKPNYHAHMIFDWTDGHTGKTVKLNRHDMAEMQTITAECLN
                     MERGVSSDKKHLSAMQYKNKMETEKAEQLQKDIEQLNRAYTAGTEKITTVQKELSTAQK
                     ELNSMKTDIHINEAKEAAAKAGKTVFNAVTSVFNVGEVKRQAKEIETLKKENYNLSFKN
                     QNLEGQLRTNNIEMRRVQETTDRQIKAEASKLKPITNLFPEMENAQENIEELQTMGIQN
                     KDIRQLLIGKEIYYTGNLYDKDKRKSYQVKNVKIDISKSQNGITTIWLNNIHFKNFLKE
                     LWNTLQKTLDNGNWLHR"
                     /name="hypothetical protein"
     gene            2497..2745
                     /locus_tag="NXV98_23775"
     CDS             2497..2745
                     /locus_tag="NXV98_23775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="UVR50258.1"
                     /translation="MVTNIIQIGNSKGIILPSEVLKQLRLSLKSAVSISLDGNNIVIKA
                     QPRQGWAEAAKRAHENGDDELLIPDVFEDEKFEDWTW"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            2739..3064
                     /locus_tag="NXV98_23780"
                     /pseudo=""
     CDS             2739..3064
                     /locus_tag="NXV98_23780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799262.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            3101..3499
                     /locus_tag="NXV98_23785"
     CDS             3101..3499
                     /locus_tag="NXV98_23785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-poly-alpha-D-galacturonosidase"
                     /protein_id="UVR50253.1"
                     /translation="MKPTDYIEWDNLKDIPFFLCQVVEDREKQDLDIYYLGKRVLHDYD
                     HVGHYLRTAVILFRRVKSRTADWVNLRNLWTLRNCVRENYNHGIGMNDLIFGENFDGDN
                     LDTLTPLTKKRFDFLCKRIKELDPYATI"
                     /name="exo-poly-alpha-D-galacturonosidase"
     gene            4141..5043
                     /locus_tag="NXV98_23790"
     CDS             4141..5043
                     /locus_tag="NXV98_23790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="UVR50254.1"
                     /translation="MKAYMDAHVCSNVYTVQGGKAHSHYCHRAFCPICQRIQTAQNVKK
                     FLPVMKYLASEGREFYFVTLTLQNCVTDDARVLRDFMRRCNRMWADGIRTKHKFRSLGI
                     SGVIKKECTYHVVKKDLFSFHYHFHIIVDSLEAAKYVVAQWKKLHGSTVADARFQKYKK
                     IEDFENAAIEVFKYASKASVSKSKGRDGKKKIQINYKALDMIYTAMHGMQRMSSFGQFR
                     TMIGDEALNDFRDEDLVLNIEGLNVEDGCYTWYMSVKDKVADWYNMETGEALCCYKVTR
                     KDELLQDIYLDEDIFPNSG"
                     /name="protein rep"
     gene            complement(5047..5508)
                     /locus_tag="NXV98_23795"
     CDS             complement(5047..5508)
                     /locus_tag="NXV98_23795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR50255.1"
                     /translation="MNRIIHISLLISTVSLFLISNTGCVEKQGYYNHGEESIISLICDI
                     TWAGKKTTDENGSVWQGTYKFNKNGTYTRTNIEIDKQGNKKEANIYGQWSFGDPSFSTI
                     YFGGEHYWDIDELTKNKFSFYDRSGKFGDPFMNREYIELTPYQENNTTN"
                     /name="hypothetical protein"
     Typing          3995..5043
                     /name="rep_cluster_870"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.81"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(845..2091)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaaaaaag taatgtttat gttcatcagt ctactgactg ttttgggtgt ttgttcgtgt
       61 aatgataatg aagtaataga acctgtggtt agtgattctg tatctgatat ggaggtctta
      121 tctcgttttg ttgatgtaaa tgagattaca aatgaatact atttcaatga gaataagaaa
      181 acgagggcgt tgtcttatgt aacaggttcg gattggcagg atttagaaaa agttagtcct
      241 ttgagtattg agaaatataa gaataacctg caggttttaa atgctcaagt ggcaagtgct
      301 atttcaaatc ctaataccgc ttatgttgtt ttttctgtaa atggaaagac tttggttaaa
      361 aaggtgaagg aggatgctaa ttttgatttt tcagtattta gggatgttgt aacggaaacg
      421 agggctgttc tcccttcttt gagtattaat ggaggttctc aaagcactac cggagttttt
      481 tacgattcca gtcgtacttt aaagatgcaa gttgatttga atgcttcaat acagaataat
      541 tattatttct ttgaagtatt aaatcctaat gctaaaccaa gtccggatga taatataacg
      601 acacctgaaa gtgttgcttt tagtggaact ggaccgttat ggagtaacac tttcacttgg
      661 acttcgtatt gggatgcaaa tgtacctggt caaggtttta aatgggagtt taaaggaaaa
      721 ggtactactc cttcttttgg ttttatagct aattgcacct ttagtcgtta aaaaatgaac
      781 gggatttaat ttgaattgca cttctcaaaa aagagaagtg caattcaaat tagtgtcttt
      841 ctatcggtgt agccagttac cgttatctaa agtcttttgt agtgtattcc aaagttcttt
      901 aagaaagttt ttaaagtgga tgttatttag ccaaattgta gtaataccat tctgactttt
      961 ggagatatct attttcacgt ttttaacttg gtatgatttt ctcttgtctt tatcgtaaag
     1021 gtttccagtg tagtatattt cctttccaat aagaagttgt cggatatctt tattttgtat
     1081 tcccatagtt tggagttcct ctatattttc ctgtgcattt tccatttccg gaaaaaggtt
     1141 ggttatgggt tttagtttgg atgcttctgc ctttatctgt ctgtcggttg tttcctgtac
     1201 ccttcgcatc tcgatgttgt tggttcttaa ttgtccttct aaattttgat tcttgaatga
     1261 gaggttataa ttttcttttt tgagggtttc aatttctttg gcttgtcgtt ttacttcccc
     1321 tacattaaag acacttgtaa cggcattaaa tacggttttt ccggcttttg cggcagcttc
     1381 tttggcttcg ttgatgtgta tgtcggtttt catgctgttt agctcttttt gtgccgtact
     1441 aagttccttt tggactgtgg tgattttctc ggttccggca gtgtatgccc tgtttagttg
     1501 ctcgatgtct ttttgaagtt gttcagcttt ctctgtttcc attttgtttt tgtattgcat
     1561 ggccgacaag tgtttcttgt ctgaagaaac tccacgttcc atgttgagac actctgctgt
     1621 tatagtctgc atctccgcca tgtcgtgtct gttcagcttt acagtttttc ctgtatggcc
     1681 gtccgtccag tcaaagatca tgtgcgcatg atagttcggt ttccatgctt cttcctttcc
     1741 gtcccatacg tttgcgcctt catccttatg ggtgtagatt tggaaggcgt gcactccaaa
     1801 gcgttcttcc agtttttcgg ccaagtcttg aagctgctgg attgtggttt cctcgctgat
     1861 tactattacc ccttcccgga ttggggtagc tttcttctgt aatccttgtc cggtggcttc
     1921 cttgtatttt tccgttatgt cccgatgtac ttcggcaatg gaacattcta cccactgttc
     1981 gttgcggtgt gtgagttccg gacggatgta gtcgagctcc ttgcttctta agttgtgacg
     2041 ttccgagctg cctatattgc aggcttccac tctgatacat gtctttgcca tatctttagg
     2101 ttttaggtgt gtatgtctgt ttagaagatg tggcgtgaaa cgtcacatcg cacggggtcg
     2161 aggggacacc cctcgcacac aaccccttta ggggttatag tgtgctataa ccttttgcaa
     2221 aggtaagcct tccggacggg atttgcaagc gctctgccat gtgttccgga tgtgcttggg
     2281 aacagaagcc ggacaaaagg gaaaaggaca caaggcacaa taaaaaatca tcggtcgggg
     2341 agcgctgcac ggcaaggggg tgcaccaaca ctcccgacga gtgaagccat tgacagggtt
     2401 ctcggtgaaa atggaaaaaa gttgtttttc cttttgttat tgttataact ttacatatat
     2461 ttgtatcgtg ataatcaatt ataaagttat aacattatgg taacgaatat tattcagatt
     2521 ggaaacagca aggggataat acttccttcc gaagtattga aacagttgcg tttgtccttg
     2581 aaatcggctg tgagcatttc tttggacggg aacaatattg tgataaaggc acaaccacgt
     2641 cagggatggg cggaagctgc caaacgtgcc catgagaatg gggatgatga gttgcttatc
     2701 cctgatgtgt ttgaggatga aaagtttgag gattggacat ggtagaacag tatgaagtgt
     2761 attgggtaga gttagaccct acccgtgggg gtgaaatggc aaagacacgg ccatgtgtag
     2821 ttgtcactcc ttcagatttg aatatgtacc ttacaacagt agtcattgtt ccgataacct
     2881 caacgataag aaattatcct taccgggttc tgtgttctgt agccgggcgt gaaggagaga
     2941 tagccactga tcaaattcgc acggtggggt ggataaaagc cggctgaaaa gaaagattgg
     3001 agatttgaat ttcagtgaga taaaaaggtt acgtgaagta tttcagcaaa tgttctgtga
     3061 ataatggaaa gtacggaaag aaaaatgcaa ataagacatt atgaaaccaa ctgactacat
     3121 cgaatgggat aacctgaagg atattccatt cttcctctgt caagtagtcg aggacagaga
     3181 aaaacaagat ttggatattt actatttggg caaacgtgtc ttgcacgatt acgaccatgt
     3241 ggggcactat ttgcggactg ccgttatctt gttccgcagg gtaaagagcc gcacagcaga
     3301 ctgggtaaac ctgcgtaacc tttggacgct gcgcaactgt gtgcgtgaga actacaatca
     3361 cggcatagga atgaacgacc ttatttttgg ggagaatttc gatggagata atctcgatac
     3421 gcttacacca cttacaaaga aacgctttga ttttttgtgt aagcggatta aggaacttga
     3481 cccatatgca actatttaac actccattga gtgaaggcag ggcttgtctt tgtgtgctcc
     3541 ccctttgggg gacgggaggg ggataagaaa aaaaatacca ccggagatgt cggatatccg
     3601 tcaattcact ccggtgtttt ttttgcaact ctcccgtatc gctcggcaag ggttgtcttt
     3661 gtgtacggca aagatagttt tttttccgga agtatctttg tctgttgtgg atttagtagt
     3721 gtatttgtgt cagaattgcg cagccggggg aatgggggcg agcccccata ctagataaac
     3781 tagaaagttt cgtggtaaat gctttttgac ttgttatatg taggtttttt gaatgatgtt
     3841 tggcggtttg gaatgaatta cctacttttg ggctgagaaa aaacgaaagc ccgaaggcag
     3901 taaccaacgg gctttgtatt ttaagaatta gcaatcgttt gtgttcgttc ttggcggggc
     3961 ggataccaaa cgaaagccac atcaaaattt catatgtgca aagatagaac aaatataacc
     4021 aactgcaaag aaagtccgtt aaaaaacggt tctgatagtc ttaaaaaaaa ggctaaagta
     4081 aaaatcatat cccataattt tactcgaaaa ctttcggaaa agaatccgga tagtaagctg
     4141 atgaaagcat atatggatgc ccatgtgtgt tcaaatgttt atactgtgca gggaggtaag
     4201 gcgcattccc attattgtca tcgggctttt tgtcctatct gtcagcgtat tcaaacggca
     4261 caaaatgtaa agaagttcct tccggttatg aagtatttgg cttcggaagg cagagagttt
     4321 tattttgtga cgcttacctt gcaaaattgt gtaaccgatg atgcaagggt attgcgagat
     4381 ttcatgcgta gatgtaaccg gatgtgggct gatggtataa ggacgaaaca caagtttcgt
     4441 tcacttggta tttcgggggt gattaagaag gagtgtacct atcatgtagt aaaaaaggat
     4501 ttgttttcgt tccattatca ttttcatatt attgttgatt cattggaagc tgcaaaatat
     4561 gtagtagccc aatggaagaa attgcatggt agtacggttg ctgatgcccg ttttcagaaa
     4621 tataagaaga tagaggattt tgaaaatgca gcgatagagg tttttaagta tgcttcaaag
     4681 gcttctgtct caaaatcgaa aggtcgtgac ggtaaaaaaa agattcagat aaattataag
     4741 gctttggata tgatttatac tgccatgcat gggatgcagc gcatgagtag tttcggacag
     4801 ttccgcacca tgatcggaga tgaagcgttg aatgatttta gagatgagga tttggttctt
     4861 aatattgagg gattgaatgt tgaagatggt tgttatactt ggtatatgtc tgtgaaagat
     4921 aaggtggctg attggtataa tatggaaact ggggaggcat tgtgttgtta taaggtcacc
     4981 cggaaggatg aacttttgca ggatatttat ttagatgagg atatttttcc taatagtggg
     5041 tagttgttag tttgttgtat tgttttcttg gtatggtgtg agttctatgt attctctatt
     5101 catgaaagga tcgccaaatt ttccacttct gtcatagaag gaaaatttgt ttttagttag
     5161 ctcgtctatg tcccagtaat gttctccacc aaaataaatg gtactgaaac ttggatcacc
     5221 gaatgaccat tgtccgtaga tattggcttc ttttttattt ccttgtttgt cgatttctat
     5281 atttgtacgg gtatatgttc cgtttttatt gaatttgtat gtaccttgcc agacactgcc
     5341 gttttcgtct gttgtttttt taccagccca tgtaatatca caaattaatg agattatgct
     5401 ttcttctcca tggttgtagt atccttgttt ttcaacacat ccggtgtttg aaataagaaa
     5461 aagtgatact gttgaaataa gtaaagagat atgtattatt ctattcatag ttaaatattt
     5521 aatattcaat gcaaaaatag gagaaatttt atatttttgg gcgaaacctt tatttattaa
     5581 ctttaaaaaa ggaggattt
//
