LOCUS       CP103201                4161 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-642 plasmid unnamed2, complete
            sequence.
ACCESSION   CP103201
VERSION     CP103201.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323513
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 189x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 21:28:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,759
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,373
            CDSs (with protein)               :: 4,373
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 289
            CDSs (without protein)            :: 289
            Pseudo Genes (ambiguous residues) :: 0 of 289
            Pseudo Genes (frameshifted)       :: 255 of 289
            Pseudo Genes (incomplete)         :: 41 of 289
            Pseudo Genes (internal stop)      :: 12 of 289
            Pseudo Genes (multiple problems)  :: 18 of 289
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-642"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="2018-12-14"
     gene            1..1026
                     /locus_tag="NXV98_23730"
     CDS             1..1026
                     /locus_tag="NXV98_23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UVR50250.1"
                     /translation="MKKKLPITKNKDVVVSWVYTWSKQQDMSIHEQRIVLRILEACQAE
                     LKGVKLKDYAGTKRKFEHGLWDVDAQMHVSDVIFSGRDYNEIIAALDSLAGRFFTYEDD
                     EEWWKCGFISNPKYKKRTGIITFRVSNDLWDVFTKFAKGYREFELNKALALPTGYSLRF
                     YMLMSGQVYPLDISLENLKDRLGIPADKYKDKNGKDRIDHFEERVLKPAKAALDESCPY
                     TFNYVKVRENPNNKRSKVTGFRFYPVYQPQFRDEELEGKELQAKVTARYQIDSHVYEYL
                     RYSCGFTSEEINRNKETFITAQEKITDLIGELALLNGKSREKNNPKGWIINALKGKIKD
                     K"
                     /name="replication initiation protein"
     gene            complement(1063..1293)
                     /locus_tag="NXV98_23735"
     CDS             complement(1063..1293)
                     /locus_tag="NXV98_23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="charged multivesicular body protein 2b-B"
                     /protein_id="UVR50251.1"
                     /translation="MGTTEHEEPRFFFILNKGAKSGGEITHAVLNGSIVSKPAGWDAFH
                     GLALAREKLSSEEIQQQMKELGVEMEIVPLI"
                     /name="charged multivesicular body protein 2b-B"
     gene            complement(1313..1764)
                     /locus_tag="NXV98_23740"
                     /pseudo=""
     CDS             complement(1313..1764)
                     /locus_tag="NXV98_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(1721..2515)
                     /locus_tag="NXV98_23745"
     CDS             complement(1721..2515)
                     /locus_tag="NXV98_23745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UVR50252.1"
                     /translation="MIGKGKSISHGVAALEYDLAKEINGQAVATEIARHELYGCTGAEM
                     VQEMKPYHIDFPNVKNNCLRFEVSPSIEESATFTDADWAELGNDFMQRMGLANHQYIII
                     RHSGTESKKEQAHLHILANRVSLSGELYRDNWIGKKATEAANAIAKERNFVQSQDIGKV
                     NKAEIKEAMDGVLKKMQGFDFTKFKEELGKRGFKVREARASTGKLNGYYVTARSGTEYK
                     ASEIGKGYTLAHIERTQSKLKCNSMNISHGNKLTPGSGSFQR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2991..3215)
                     /locus_tag="NXV98_23750"
     CDS             complement(2991..3215)
                     /locus_tag="NXV98_23750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR50247.1"
                     /translation="MVEYCVYWLENGEPMHEVFSSLAAAEMYSCAIRGKENIEWVEVSE
                     EETIDLDELEDMFPDDFCGMTSAACNPLG"
                     /name="hypothetical protein"
     gene            complement(3226..3501)
                     /locus_tag="NXV98_23755"
     CDS             complement(3226..3501)
                     /locus_tag="NXV98_23755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004519 - endonuclease activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family toxin"
                     /protein_id="UVR50248.1"
                     /translation="MMYTIRVSDGVDKVIAKWKKSNPNLFKKYKKIYKELLEHPKTGLG
                     HPEALRGGGDITWSRHITAHDRIIYDIYEEVVEVYILEVEGHYNDK"
                     /name="type II toxin-antitoxin system YoeB family toxin"
     gene            complement(3498..3749)
                     /locus_tag="NXV98_23760"
     CDS             complement(3498..3749)
                     /locus_tag="NXV98_23760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR50249.1"
                     /translation="MEALSVREYRNNLAASFTKADNGEQVLIRRKNEIYALVKVGREDL
                     MITPELQARIDKAREEIKSGKCVTLKSSEDIDAYFDSL"
                     /name="hypothetical protein"
ORIGIN
        1 atgaaaaaga aactacctat taccaagaat aaagatgttg ttgtttcatg ggtatataca
       61 tggtcaaaac agcaagacat gtccatacac gaacaaagaa tagttcttcg catactggaa
      121 gcgtgccaag ctgaactaaa gggagtaaaa ctgaaagatt atgcaggcac gaaacgcaag
      181 tttgagcatg gactttggga tgttgatgca caaatgcatg tatctgacgt tattttttcc
      241 ggacgtgatt acaatgaaat catagccgca ctcgattctt tggcaggacg tttttttact
      301 tacgaagatg acgaggaatg gtggaagtgc ggatttatat ctaacccaaa atataaaaaa
      361 cgcactggca ttataacctt tcgtgtgtct aacgaccttt gggatgtgtt taccaagttc
      421 gccaaggggt acagagaatt tgagttaaac aaggctcttg cgctacctac tgggtattcc
      481 cttaggtttt acatgttaat gagtgggcag gtgtaccctt tggatatatc ccttgaaaac
      541 ttgaaagacc gtcttggcat acctgcggac aaatacaagg acaagaacgg aaaagacaga
      601 atagatcatt ttgaggaaag agttttgaaa ccagcaaagg ctgcgcttga tgagagctgc
      661 ccatacactt tcaactacgt gaaagtgcgt gaaaacccaa acaacaaacg tagcaaggtg
      721 accgggttta ggttctaccc ggtctatcaa ccacagttca gagatgaaga actggaaggg
      781 aaagaattgc aagcaaaggt tacagcacga tatcagatag acagccatgt gtacgagtac
      841 ctccgttatt cctgcggctt cacatcggaa gagataaacc ggaacaaaga gacattcata
      901 acggcacaag aaaagataac cgaccttatc ggagaactgg ctctcctaaa cggaaaatca
      961 cgagaaaaga acaatccgaa agggtggatt ataaacgccc ttaaagggaa aatcaaggat
     1021 aagtaacaag attacccggc tgaaacactc cgggtaatct tgtcaaatca gaggaacgat
     1081 ttccatttct acaccaagtt ctttcatctg ctgttgtatt tcctcgctgc ttagtttctc
     1141 tctcgccagt gcaagcccat gaaaggcgtc ccagcctgcc ggcttcgaaa cgatactacc
     1201 gtttaggacg gcatgggtga tttcgccgcc ggacttcgca cccttgttca gtatgaagaa
     1261 gaaccggggt tcttcgtgtt ccgttgttcc cataattcgt aaagttagat gtttatttat
     1321 tccccttttg ttcccttgcc tgctcttggt agtattcata atactgcttg tagttgtccc
     1381 ggtctttggc tgcatccgtc caaagatagc acatgaaaca agcgaacaat agggagataa
     1441 cagcacaaag gattgagtag aacgaaattc gtttgtccac ataaccgccg tttttcattc
     1501 tgtacccctg ccgttcaaag tccgccaaca cgttttttag gcgattttcg gcactttcaa
     1561 ggcttttccc gtctatttgt acctttcggg ctgttgcgtc ctctatgcgc ttcgtataga
     1621 cttcaacacg ctttagttct tgatacacca gttctaccgc aagctgcggg tttacccttg
     1681 gcgttgtatc ttttcttttc gggggctgta ctgtccctgc ttaacgttgg aagctgccgc
     1741 ttccgggtgt gagtttgttt ccatgagata tgttcattga gttgcacttc agtttgcttt
     1801 gtgtccgctc gatatgggcg agagtgtagc ctttccctat ctcgcttgcc ttatactccg
     1861 taccgcttcg ggctgtgaca tagtagccgt taagtttccc ggtgcttgcc ctggcttccc
     1921 ggactttaaa acctctctta ccaagttctt ccttgaattt ggtaaagtca aagccctgca
     1981 tcttcttcaa tacgccgtcc atggcttcct tgatttccgc tttgttgact ttgccaatgt
     2041 cctgcgactg tacaaagttc cgttccttgg ctatggcatt ggcggcttcc gttgcctttt
     2101 tccctatcca gttgtcccgg tacagttccc cggaaaggga aacccggttt gccagtatgt
     2161 gcaggtgggc ttgctctttc ttgctctccg taccgctgtg ccggatgatg atgtactggt
     2221 ggttcgccaa ccccatgcgc tgcatgaagt cgttaccaag ttccgcccag tccgcatccg
     2281 taaaggtggc gctttcctct atcgaggggc tgacctcgaa acgtaggcag ttgtttttta
     2341 cgttcggaaa atcaatgtga tacggtttca tttcctgcac catttccgca ccagtacacc
     2401 cataaagttc atgccgggct atctcggtgg ctactgcctg cccgtttatc tctttggcga
     2461 ggtcgtactc caatgccgcc acaccatgtg atatgctttt ccctttccct atcatcgtga
     2521 gagtattttt ttgagctctg taattagtgc ccggttctcc tcgaacactt cccggtactg
     2581 gcgaccaccg aagtagttgt tcagccgttg gagcgttcct ttcaactggg ctatgtcccg
     2641 gagaagctgc cgttcctcct cggtgtacct ctcccttggg cgccccccaa gggacaaact
     2701 ccggcagtat tccgatacgc ttagactgca ccgggctgcc tgctccgcaa gtgcgtcctt
     2761 ttcaagttcg gtacaccgga atgtaatctt ttcgttccgt cttagttcca tgccacaaag
     2821 gtaatcttaa aatctttttg agcaaagcga gaaaagcaag aattgtccga caaggacact
     2881 tcttgctata tatacacatg ccgcatggga atacatccac gccaaccgcc cagcaaaaag
     2941 ccccggcgtg ttagaaaatc ccccacagcc taacaaattc taccccattt ctacccaagg
     3001 ggattacacg ccgcagaagt catcccgcag aagtcatccg ggaacatgtc ttccagttcg
     3061 tccaaatcaa tggtttcttc ttcggacacc tccacccatt caatgttttc tttccctctt
     3121 atcgcacatg agtacatctc ggcggcggca agagaagaga acacctcgtg cataggctcg
     3181 ccgttctcta accagtaaac acaatattcg accataacag ttttatcatt tatcgttgta
     3241 atgcccctct acctccaaga tgtagacctc taccacttcc tcgtaaatgt catagattat
     3301 ccggtcatgg gcggtaatgt gtcgagacca tgtaatatcc ccaccgcccc ttaacgcttc
     3361 gggatgccca agcccagtct tagggtgctc taacaattct ttgtagattt tcttatactt
     3421 cttgaacaaa ttggggtttg atttcttcca tttggcaatc accttatcca ccccgtcgga
     3481 aaccctaatt gtgtacatca tagactatcg aaataagcgt caatatcctc actgcttttg
     3541 agggtaacac actttccgga tttgatttct tcccttgcct tgtcaatcct tgcttgcagc
     3601 tccggggtta tcatcaaatc ttcacgacca acttttacca aagcgtaaat ctcgtttttc
     3661 ctgcgaatta gtacctgctc gccattgtca gctttggtga aagacgctgc gaggttgtta
     3721 cggtattctc ttactgataa tgcttccata tctgtttgct tttaaattca gcacaaagat
     3781 agctatattt caataaaata caaacatttt gtacacaaac gtgtacacgc cataaaaacc
     3841 cgtttccaat cctaccgccc gttggttggt tttgctttgc tctttttccc tatcgttttt
     3901 ctttttccga cagtaaatca accacttagc tctataaatt ccccttgtct gttataagca
     3961 tcgtctgtaa tatgctctat cacttcttcg caaacgccca taaataaagg gattagaaca
     4021 tgtgtttccc cacactttgg gagtttagtc cccacacttt gggagtttaa aagtaaatta
     4081 gtccccacac tttgggagtt ttacccctct ttttttgttt taatgtgggg attgtgtata
     4141 tttgcagcaa atcaatcgtt t
//
